Current cellular biochemistry最新文献

Analisis Tegangan Pada Bone Plate Stainless Steel 316L untuk Aplikasi Biomateria

Current cellular biochemistry Pub Date : 2023-09-01 DOI: 10.29244/cb.10.1.3

Ahmad Fikri, Agam Muarif, Rizka Mulyawan, Nursakinah

{"title":"Analisis Tegangan Pada Bone Plate Stainless Steel 316L untuk Aplikasi Biomateria","authors":"Ahmad Fikri, Agam Muarif, Rizka Mulyawan, Nursakinah","doi":"10.29244/cb.10.1.3","DOIUrl":"https://doi.org/10.29244/cb.10.1.3","url":null,"abstract":"Biomaterial adalah material yang digunakan untuk mengganti struktur biologis yang hilang atau sakit. Material ini harus dapat diterima oleh tubuh. Salah satu biomaterial yang paling banyak digunakan untuk aplikasi bone plate adalah stainless steel 316L. Saat diaplikasikan pada tubuh pasien, material ini akan mendapatkan beban sejajar permukaan. Beban ini merupakan beban yang cukup dominan diterima oleh bone plate stainless steel 316L. Beban yang diterima bone plate stainless steel 316L akan berubah dan bervariasi. Sehingga perlu dilakukan simulasi tegangan degan memberikan gaya tekan pada bone plate stainless steel 316L. Simulasi tegangan dilakukan dengan menggambar dan menganalisis tegangan dengan menggunakan Autodesk Fusion 360. Selanjutnya material tersebut diberikan beban berupa static load mulai dari 150 N sampai 2300 N. Hasil analisis tegangan menunjukkan distribusi tegangan terdapat ditengah dan ujung bone plate. Regangan dan perubahan bentuk menunjukkan peningkatan seiring dengan kenaikan nilai tegangan. Gaya tekan 150 N sampai 600 N merupakan beban yang aman karena masih dibawah titik luluh 170 MPa. Gaya tekan 700 N sampai 2300 N merupakan beban yang tidak aman karena sudah melebihi titik luluhnya. Hal ini akan menyebabkan material mengalami deformasi plastis dan patah.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83353336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skrining Fitokimia, Kadar Total Flavonoid dan Antioksidan Daun Cocor Bebek

Current cellular biochemistry Pub Date : 2023-07-30 DOI: 10.29244/cb.10.1.1

Eka Nurul Qomaliyah, N. Indriani, Atika Rohma, Ricka Islamiyati

引用次数: 0

Uji Antibakteri Bakteri Endofit Jawer Kotok (Coleus scutellarioides L. Benth) Terhadap Bakteri Bacillus cereus dan Salmonella enteritidis

Current cellular biochemistry Pub Date : 2023-07-29 DOI: 10.29244/cb.10.1.2

Dwi Endah Kusumawati, M. Bintang

{"title":"Uji Antibakteri Bakteri Endofit Jawer Kotok (Coleus scutellarioides L. Benth) Terhadap Bakteri Bacillus cereus dan Salmonella enteritidis","authors":"Dwi Endah Kusumawati, M. Bintang","doi":"10.29244/cb.10.1.2","DOIUrl":"https://doi.org/10.29244/cb.10.1.2","url":null,"abstract":"The use of synthetic antibiotics in small doses and long term against pathogenic bacteria can cause negative effects, such as resistance of pathogens and killing of good bacteria. We needs an innovation to substitute the use of synthetic antibiotics with natural antimicrobial compounds. Jawer kotok (Coleus scutellarioides L. Benth) has been used as medicinal plant and shown to be efficacious as antimicrobial because it contains flavonoids, tannins, alkaloids and saponins. Endophytic bacteria are symbiotic microorganisms that live in healthy plant tissue without harming to the host plant and may produce natural antimicrobial compounds which similar to the host plant. The aim of this study are to isolating endophytic bacteria from jawer kotok and screening their antibacterial activity against two pathogenic bacteria, namely Bacillus cereus and Salmonella enteritidis. A total of 22 bacterial isolates were obtained from C scutellarioides [L.] Benth. Based on antibacterial activity test, nine isolates shown antibacterial activity against B. cereus and S.enteritidis. BJ7 is the most potential isolate because it shown the biggest inhibition. It great possible that endophytic bacteria from jawer kotok may synthesize the same antimicrobial compounds like their host plant.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80555228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetylcholinesterase Enzyme Inhibitor and Antioxidant Activities from A Mixture Extracts of Black Tea, Red Betel, Cinnamon and Curcuma 红茶、红槟榔、肉桂和姜黄混合提取物的乙酰胆碱酯酶抑制剂及其抗氧化活性

Current cellular biochemistry Pub Date : 2023-01-24 DOI: 10.29244/cb.9.2.2

Aziz Syamsul Huda, A. Hasan, M. Safithri

{"title":"Acetylcholinesterase Enzyme Inhibitor and Antioxidant Activities from A Mixture Extracts of Black Tea, Red Betel, Cinnamon and Curcuma","authors":"Aziz Syamsul Huda, A. Hasan, M. Safithri","doi":"10.29244/cb.9.2.2","DOIUrl":"https://doi.org/10.29244/cb.9.2.2","url":null,"abstract":"Alzheimer's Disease (AD) is one of the consequences of impaired functioning of acetylcholine which can be hydrolyzed by the enzyme acetylcholinesterase. Alzheimer's treatment is carried out using two approaches, namely compounds (acetylcholinesterase inhibitors) and noncholinergics (antioxidants). There are commercial drugs that can slow the progression of Alzheimer's, but their use can cause excessive side effects. The use of herbal plants as a cure for Alzheimer's disease has been proven to be safer and does not cause excessive side effects. Herbal plants that can be used and developed are black tea, red betel, curcuma, and cinnamon. This study determined that extracts of black tea, red betel leaf, curcuma, and cinnamon's formula have the best antioxidant activity and level of inhibition of the acetylcholinesterase enzyme. F1 (cinnamon), F5 (cinnamon, black tea, red betel, and curcuma), F6 (cinnamon and black tea), and F11 (cinnamon, black tea, and curcuma) were the best formulations in each type of sample based on inhibitory AChE enzymes and antioxidants activity. F1 (cinnamon) is the most effective extract out of all formulations analyzed in this study. \u0000 \u0000Keywords: Acetylcholinesterase; Alzheimer; Black Tea; Cinnamon; Red Betel; Curcuma","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79010124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sprague Dawley Rat Spermatozoa Quality Due to Bee Pollen Consumption 蜂花粉消耗对大鼠精子质量的影响

Current cellular biochemistry Pub Date : 2022-12-31 DOI: 10.29244/cb.9.2.4

A. Hasan, Moerfiah, Septa Fitri Handayani

{"title":"Sprague Dawley Rat Spermatozoa Quality Due to Bee Pollen Consumption","authors":"A. Hasan, Moerfiah, Septa Fitri Handayani","doi":"10.29244/cb.9.2.4","DOIUrl":"https://doi.org/10.29244/cb.9.2.4","url":null,"abstract":"Bee Pollen is a food that has a complete nutritional content including as a source of steroid hormones, helping to improve concentration and brain performance. This study aims to determine the effect of bee pollen on the sperm quality of male rats. Administering 1 ml of water as control (-), 1 ml/kg of Eurycoma longifolia capsule as control (+), 1 ml/kg of nano bee pollen, and bee pollen with concentrations of 0.33 ml/kg, 0.66 ml/kg, 1 ml/kg, and 1.33 ml/kg 34 times in Sprague Dawley male white rats. The mice were then operated on and examined for testicular weight, epididymal weight, sperm pH, sperm motility, sperm concentration, and sperm abnormalities. The results showed that bee pollen and nano bee pollen did not significantly affect the quality of rat spermatozoa. In examining the testicular weight of rats, 0.33 ml/kg of bee pollen had an average testicular weight greater than the control group. For sperm weight and pH of the rat epididymis, the administration of bee pollen showed the average weight and pH were lower than the control. In the examination of sperm motility, 0.33 ml of bee pollen had a higher percentage than the control, while 1 ml of nano bee pollen had the lowest percentage of motility. The results of the concentration and abnormalities of rat spermatozoa from a dose of 1 ml/kg of bee pollen had an average sperm count higher than the control which was 2,129.7 million/ml and the lowest percentage of sperm abnormalities was 8.73%. \u0000 ","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"99 1-4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83363283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of Active Compounds in Leaves, Seeds, and Peel of Avocado Fruit (Persea americana Mill.) as In Silico Tyrosinase Enzyme Inhibitors 牛油果(Persea americana Mill.)叶片、种子和果皮中活性化合物作为硅酪氨酸酶抑制剂的潜力

Current cellular biochemistry Pub Date : 2022-12-31 DOI: 10.29244/cb.9.2.3

P. Puspita, Ni Putu Peggy Liliyani, L. Ambarsari

{"title":"Potential of Active Compounds in Leaves, Seeds, and Peel of Avocado Fruit (Persea americana Mill.) as In Silico Tyrosinase Enzyme Inhibitors","authors":"P. Puspita, Ni Putu Peggy Liliyani, L. Ambarsari","doi":"10.29244/cb.9.2.3","DOIUrl":"https://doi.org/10.29244/cb.9.2.3","url":null,"abstract":"Melanocytes are an important component of skin pigmentation through their ability to produce melanin assisted by the enzyme tyrosinase which is a core enzyme in melanogenesis. The high phenolic and flavonoid compounds in the leaves, seeds and skin of avocados have potential as ligands (inhibitors) of tyrosinase related protein-1. This study aims to determine the active compounds from the leaves, seeds, and skin of avocados that play a role in inhibiting the activity of the tyrosinase enzyme in humans so that it can inhibit the formation of melanin. The research was conducted by molecular anchoring method using YASARA Structure software. The results showed that of the 68 active compounds of avocado leaves, seeds, and skins, there were 11 active compounds that had binding affinity for tyrosinase related protein-1, namely quarcetin, epicatechin, apigenin, 3-Hydroxytyrosol, scopoletin, abscisic acid, citric acid, coumaric acid. , quinic acid, vanillin, and caffeic acid. The active compound abscisic acid is the ligand with the best inhibitory potential with a bond free energy (∆G) of -2,645 kcal/mol which interacts with 17 amino acid residues. The research results can be used as a reference in in vitro and in vivo studies of the human tyrosinase enzyme. \u0000 ","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80809157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analisis In Silico Senyawa Aktif Sirih Merah (Piper crocatum) sebagai Inhibitor Xantin Oksidase

Current cellular biochemistry Pub Date : 2022-12-31 DOI: 10.29244/cb.9.2.1

Mega Safithri, Shafillah Miantika, L. Ambarsari

引用次数: 0

A Brief Overview and History of Human Nutrition and Health 人类营养与健康简史概述

Current cellular biochemistry Pub Date : 2022-12-31 DOI: 10.29244/cb.9.2.5

H. Ahsan

引用次数: 0

The Inhibitory Activity and Kinetics of α-Glucosidase by Toona sinensis Stem Bark Extracts 香椿茎皮提取物对α-葡萄糖苷酶的抑制活性及动力学研究

Current cellular biochemistry Pub Date : 2022-06-10 DOI: 10.29244/cb.9.1.2

Melati Devina Gustini Wirastuti, Syamsul Falah, Syaefudin

{"title":"The Inhibitory Activity and Kinetics of α-Glucosidase by Toona sinensis Stem Bark Extracts","authors":"Melati Devina Gustini Wirastuti, Syamsul Falah, Syaefudin","doi":"10.29244/cb.9.1.2","DOIUrl":"https://doi.org/10.29244/cb.9.1.2","url":null,"abstract":"Research on the potential of the Surian tree (Toona sinensis) for medicine has been carried out, especially for the leaves. However, the bark section has not been done much. This study aimed to determine the inhibitory activity of -glucosidase and IC50 extract as well as the fraction of surian bark as antidiabetic in vitro. The reflux method with water at 90oC and maceration using 70% ethanol at room temperature was carried out to obtain aqueous and ethanol extracts. Furthermore, the ethanol extract was fractionated successively using n-hexane, diethyl ether, and ethyl acetate. Phytochemical screening test showed that the aqueous extract contained flavonoids, saponins, and tannins. Meanwhile, the 70% ethanol extract and its fraction showed terpenoid content, in addition to flavonoid compounds, saponins, and tannins. The best -glucosidase inhibition was shown by 70% ethanol extract, which was 70.45 ppm followed by ethyl acetate fraction, n-hexane fraction, diethyl ether fraction, and water extract of surian bark with values of 81.96 g/mL, 98.76 g/mL, respectively. , 140.25 g/mL, and 242.85 g/mL. Kinetics of -glucosidase inhibition showed that 70% ethanol extract of surian bark inhibited through a non-competitive inhibition mechanism, characterized by a decrease in Vmax from 8.29x10-3 mM/min to 1.65x10-3 mM/min and a decrease in Km from 0.17 mM to 0.03 mM.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80132543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatoprotective Activity of Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in Rats Induced by Paracetamol 蜂胶、芙蓉、蜜蕊对扑热息痛大鼠的保肝作用

Current cellular biochemistry Pub Date : 2022-06-10 DOI: 10.29244/cb.9.1.4

A. Hasan, A. Setiyono, Miko Afrian

{"title":"Hepatoprotective Activity of Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in Rats Induced by Paracetamol","authors":"A. Hasan, A. Setiyono, Miko Afrian","doi":"10.29244/cb.9.1.4","DOIUrl":"https://doi.org/10.29244/cb.9.1.4","url":null,"abstract":"Negative effects on health will occur in excessive use of paracetamol. The main objective of this study was to determine the hepatoprotective activity of Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in rats induced by paracetamol. The study was conducted on 36 Sprague Dawley rats with 6 rats in each group. Group 1 (normal control), group 2 (positive control, temulawak 42.86 mg/kg BW), group 3 (negative control), group 4 (infusion of Myrmeleon 10 mg/kg BW), group 5 (propolis extract 0.02 ml/kg BW), and group 6 (Hibiscus sabdariffa infusion 0.02 ml/kg BW). Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations were determined spectrophotometrically and microscopically for liver histopathology. The results showed that during three weeks of treatment with Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in rats, this treatment could decrease ALT and AST activity as the positive control. The ALT activity values of the three materials were 69.26, 51.00, 51.00, 43.67, U/I with AST values of 110.29, 97.33, 91.00, 91.33 U/I. Based on Duncan's test, there were significant differences between all treatments with negative and positive control groups. Likewise, with the histopathological scoring results, the negative control was found to be significantly different from all treatments. Therefore, treatment with propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. may have hepatoprotective benefit. \u0000 ","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80189701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0