Skrining Fitokimia, Kadar Total Flavonoid dan Antioksidan Daun Cocor Bebek

Current cellular biochemistry Pub Date : 2023-07-30 DOI:10.29244/cb.10.1.1

Eka Nurul Qomaliyah, N. Indriani, Atika Rohma, Ricka Islamiyati

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Abstract

Kalanchoe pinnata Linn. or in Indonesia known as cocor bebek, this plants grow well in temperate and tropical regions. Cocor bebek is used to treat several diseases including skin diseases, wound healing, kidney stones, gallstones, diabetes. The pharmacological activities of cocor bebek leaves have been reported as anti-inflammatory, anti-oxidant and anti-microbial. The objective of this study is to determine the metabolite content of cocor bebek leaves, total flavonoid levels and antioxidant activity. Observations of secondary metabolites of cocor bebek leaves were carried out through qualitative secondary metabolite screening, while total flavonoid levels were carried out quantitatively UV-VIS with quercetin as a comparison, atioksidan activity through DPPH molecular inhibition testing. The results of this study indicate that cocor bebek leaves contain alkaloids, flavonoids, saponins, triterpenoids, tannins and phenols. 200gr of cocor bebek leaves obtained % yield extract is 14.63%. The total favonoid content obtained was 8.77% of quercetin, while the antioxidant inhibition of DPPH molecules obtained IC50 of 97.42 ppm.

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凤尾鱼林。或在印度尼西亚被称为coor bebek，这种植物在温带和热带地区生长良好。可用于治疗多种疾病，包括皮肤病、伤口愈合、肾结石、胆结石、糖尿病。豆豆叶具有抗炎、抗氧化、抗微生物等药理作用。本研究的目的是测定黄豆叶的代谢物含量、总黄酮水平和抗氧化活性。通过定性的次生代谢物筛选对山茱萸叶的次生代谢物进行观察，同时以槲皮素为对照对总黄酮水平进行紫外-可见定量测定，通过DPPH分子抑制试验对硫丁苷活性进行测定。结果表明，黄酮类化合物、三萜、单宁、酚类化合物、生物碱、黄酮类化合物、皂苷类化合物等均含有黄酮类化合物。200g山茱萸叶的提取率为14.63%。总黄酮类化合物含量为槲皮素的8.77%，抗氧化抑制DPPH分子的IC50为97.42 ppm。

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Current cellular biochemistry

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