Current cellular biochemistry最新文献_第4页

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds 马钱子乙醇提取物抗氧化活性、抑制α-葡萄糖苷酶活性及活性成分鉴定

Current cellular biochemistry Pub Date : 2019-03-12 DOI: 10.29244/CB.5.3.11-20

Stefani Dhale Rale, H. Hasim, S. Falah

{"title":"Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds","authors":"Stefani Dhale Rale, H. Hasim, S. Falah","doi":"10.29244/CB.5.3.11-20","DOIUrl":"https://doi.org/10.29244/CB.5.3.11-20","url":null,"abstract":"This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"214 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79410985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Toxicity Extract and Faction of Surian Toona sinensis Leaf and Bark against Shrimp Larvae Artemia salina L. 香椿叶、皮提取物及其对虾仔鱼的毒力研究。

Current cellular biochemistry Pub Date : 2019-03-04 DOI: 10.29244/CB.3.3.128-137

Rori Theresia, S. Falah, M. Safithri, Muhammad Assyar

引用次数: 2

The Usage of Momordica charantia as Face Cleansing Cosmetics: Momordica charantia Leaves Extract as Absorbent of Heavy Metal, Surface Tension Reducer and Skin Antibacterial 苦瓜作为洁面化妆品的用途:苦瓜叶提取物具有重金属吸收剂、表面张力减减剂和皮肤抗菌作用

Current cellular biochemistry Pub Date : 2019-03-04 DOI: 10.29244/CB.3.3.138-144

D. Andrianto, Popi Asri Kurniatin, Silvy Aulya, Dewi Eriyanti

{"title":"The Usage of Momordica charantia as Face Cleansing Cosmetics: Momordica charantia Leaves Extract as Absorbent of Heavy Metal, Surface Tension Reducer and Skin Antibacterial","authors":"D. Andrianto, Popi Asri Kurniatin, Silvy Aulya, Dewi Eriyanti","doi":"10.29244/CB.3.3.138-144","DOIUrl":"https://doi.org/10.29244/CB.3.3.138-144","url":null,"abstract":"Momordica charantia (daun pare in Indonesian) is one of Indonesian herbal plants which useful property has not been explored. M. charantia contains chemical substances such as oil and saponin. Nowadays, the society possesses a high awareness regarding the risk of using artificial chemicals for skin care and more people are switching to use herbal cosmetics. The extract of M. charantiahas been proven to be able to be used as one of the active components of herbal based facial cleansing cosmetics. Its active properties include its ability to absorb heavy metal from air pollution and motor vehicles emission, decrease surface tension, and to act as an antibacterial agent.In the research, M.charantia was extracted using 4 (four) solvents, namely water, ethanol, methanol, and hexane. The research then examined the property of each extract in metal absorption (using Atomic Absorption Spectrophotometry / AAS), decreasing surface tension, and antibacterial activities. The result of the test showed that the water based extract was the most effective extract in reducing surface tension and in inhibiting the development of Escherichia coli and Staphylococcus aureus bacteria, while the ethanol based extract was the most effective one in absorption of Pb and Hg metals and in inhibiting the development of Staphylococcus epidermidis bacteria. The result indicated that M. charantiaextract has the potentials to be used as facial cleanser and can also counter the bacterial infection which could cause acnes.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75615039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Activity and Phytochemical Analysis of Geranium homeanum Turez Leaves 天竺葵叶片抑菌活性及植物化学分析

Current cellular biochemistry Pub Date : 2019-03-04 DOI: 10.29244/CB.4.3.13-22

Fri Rahmawati, M. Bintang, I. Artika

{"title":"Antibacterial Activity and Phytochemical Analysis of Geranium homeanum Turez Leaves","authors":"Fri Rahmawati, M. Bintang, I. Artika","doi":"10.29244/CB.4.3.13-22","DOIUrl":"https://doi.org/10.29244/CB.4.3.13-22","url":null,"abstract":"Geranium homeanum Turez is a herbaceous plant used as an empirical medicine. This research was carried out to test the antibacterial activity and determine minimum inhibition concentration (MIC) to Staphylococcus aureus and Pseudomonas aeruginosa with agar diffution method and phytochemical analysis of geranium leaves by Harbone method. The young and old geranium leaves were blended then filtrated. The obtained filtrate was divided into two parts, one part heated by autoclave and the other was unheated. Each filtrate was tested against to Staphylococcus aureus and Pseudomonas aeruginosa. Geranium leaves filtrate having the highest antibacterial activity was heated at 500C until it become dry powder. The powder was used to measure MIC and phytochemical analysis.The results showed that the antibacterial activity of young leaves filtrate was higher than the old leaves filtrat, and the unheated leaves filtrate was higher than heated filtrate one. MIC of S. aureus and P. aeruginosa, were as follows 15 mg/ml and 20 mg/mL respectively. The antibacterial activity of powder geranium’s leaves filtrate was weaker than 100 µg/mL ampicilline. Phytochemical analysis of geranium leaves showed positive contents of alkaloid and triterpenoid.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90470505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Antibacterial Activities of Sirih Merah (Piper crocatum) Leaf Extracts 枇杷叶提取物的抑菌活性研究

Current cellular biochemistry Pub Date : 2019-02-28 DOI: 10.29244/CB.5.3.1-10

P. Puspita, M. Safithri, Nirmala Peni Sugiharti

{"title":"Antibacterial Activities of Sirih Merah (Piper crocatum) Leaf Extracts","authors":"P. Puspita, M. Safithri, Nirmala Peni Sugiharti","doi":"10.29244/CB.5.3.1-10","DOIUrl":"https://doi.org/10.29244/CB.5.3.1-10","url":null,"abstract":"Piper crocatum is one of medicinal herbal plants with a large number of benefits. Usually herbal plants have activity as antibacterial agent. Therefore, the objectives of this research were to obtain information on antibacterial activities of the leaf extracts of Piper crocatum againts four types of bacteria, in that Staphylococcus, Bacillus substilis, Escherichia coli, and Pseudomonas aeruginosa and then to analyze the phytochemistry of the leaf extracts of Piper crocatum. The leaves of Piper crocatum were extracted by maceration and reflux using ethanol 30%. The assays of the antibacterial activities and phytochemistry on the extracts were carried out using the method of Maria Bintang. Results showed that the yield of the extraction using ethanol by maceration method was 20.8%. Meanwhile, using the reflux method, the yield was obtained about 26.25%. The phytochemistry analysis showed that the leaf extracts of Piper crocatum contained alkaloid, steroid and tanin. According to this study, it was found that the leaf extract of Piper crocatum can be used to inhibit the growth of B. subtilis and P. aeuruginosa, but can not inhibit the growth of E.coli and S. aureus.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72931637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Antioxidant Status of Sprague-Dawley Female Rat with Curcuminoids Nanoparticles of Balittro Curcuma 姜黄素纳米颗粒对Sprague-Dawley雌性大鼠抗氧化作用的影响

Current cellular biochemistry Pub Date : 2017-04-01 DOI: 10.29244/CB.4.1.15-37

L. Ambarsari, Riska A. Febrianti, Edy Djauhari Purwakusumah

引用次数: 0

Inhibition of Rab5 Activation During Insulin Receptor-Mediated Endocytosis. 胰岛素受体介导的内吞作用中Rab5活化的抑制作用。

Current cellular biochemistry Pub Date : 2011-12-28

Ivan Jozic, Gustavo Blanco, M Alejandro Barbieri

{"title":"Inhibition of Rab5 Activation During Insulin Receptor-Mediated Endocytosis.","authors":"Ivan Jozic, Gustavo Blanco, M Alejandro Barbieri","doi":"","DOIUrl":"","url":null,"abstract":"Activation of receptor tyrosine kinases is a key feature in receptor signaling and membrane trafficking processes. In this study, we found that the insulin receptor tyrosine kinase activity is required for fusion between early endosomes. AG1024, a receptor tyrosine kinase inhibitor, blocked the in vitro endosome fusion in a concentration-dependent manner. We observed that Rab5: wild type partially rescued the fusion reaction, whereas Rab5: Q79L mutant fully rescued it. We also observed that treatment of cells with insulin receptor kinase inhibitor HNMPA-(AM)3 blocked the formation of Rab5-positive endosomes as well as the activation of Rab5 upon addition of insulin in intact cells. HNMPA-(AM)3 inhibitor also affected the endosomal co-localization of Rab5 and insulin receptor. However, the formation of Rab5: Q79L mutant-positive endosomes were not affected by the HNMPA-(AM)3 inhibitor. In addition, HNMPA-(AM)3 inhibitor affected the association of Rin1 to membrane upon insulin stimulation. Furthermore, Rin1 did not fully support endosome fusion in the presence of the AG1024 inhibitor. These results constitute the first evidence that, at least in part, the enzymatic activity of insulin receptor is required for the fusion events via the activation of Rab5.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"1 1","pages":"20-32"},"PeriodicalIF":0.0,"publicationDate":"2011-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995085/pdf/nihms395612.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32291659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential nuclear staining assay for high-throughput screening to identify cytotoxic compounds. 用于高通量筛选鉴定细胞毒性化合物的差异核染色测定法。

Current cellular biochemistry Pub Date : 2011-01-01 Epub Date: 2011-09-12

Carolina Lema, Armando Varela-Ramirez, Renato J Aguilera

{"title":"Differential nuclear staining assay for high-throughput screening to identify cytotoxic compounds.","authors":"Carolina Lema, Armando Varela-Ramirez, Renato J Aguilera","doi":"","DOIUrl":"","url":null,"abstract":"As large quantities of novel synthetic molecules continue to be generated there is a challenge to identify therapeutic agents with cytotoxic activity. Here we introduce a Differential Nuclear Staining (DNS) assay adapted to live-cell imaging for high throughput screening (HTS) that utilizes two fluorescent DNA intercalators, Hoechst 33342 and Propidium iodide (PI). Since Hoechst can readily cross cell membranes to stain DNA of living and dead cells, it was used to label the total number of cells. In contrast, PI only enters cells with compromised plasma membranes, thus selectively labeling dead cells. The DNS assay was successfully validated by utilizing well known cytotoxic agents with fast or slow cytotoxic activities. The assay was found to be suitable for HTS with Z' factors ranging from 0.86 to 0.60 for 96 and 384-well formats, respectively. Furthermore, besides plate-to-plate reproducibility, assay quality performance was evaluated by determining ratios of signal-to-noise and signal-to-background, as well as coefficient of variation, which resulted in adequate values and validated the assay for HTS initiatives. As proof of concept, eighty structurally diverse compounds from a small molecule library were screened in a 96-well plate format using the DNS assay. Using this DNS assay, six hits with cytotoxic properties were identified and all of them were also successfully identified by using the commercially available MTS assay (CellTiter 96® Cell Proliferation Assay). In addition, the DNS and a flow cytometry assay were used to validate the activity of the cytotoxic compounds. The DNS assay was also used to generate dose-response curves and to obtain CC50 values. The results indicate that the DNS assay is reliable and robust and suitable for primary and secondary screens of compounds with potential cytotoxic activity.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"1 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141097024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0