Current cellular biochemistry最新文献_第3页

Antibacterial Activity of Lemongrass (Cymbopogon nardus) Ethanolic Extract 柠檬草乙醇提取物的抑菌活性研究

Current cellular biochemistry Pub Date : 2020-11-19 DOI: 10.29244/cb.6.2.4

W. Nurcholis, M. Takene, Ratna Puspita, Lisnawati Tumanggor, Eka Nurul Qomariyah, Muhammad Maftuchin Sholeh

引用次数: 1

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro 正己烷提取物和绿茶提取物对MCM-B2乳腺癌细胞的体外抗增殖作用

Current cellular biochemistry Pub Date : 2020-11-19 DOI: 10.29244/cb.6.2.3

Lisni Noraida Waruwu, M. Bintang, B. Priosoeryanto

{"title":"N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro","authors":"Lisni Noraida Waruwu, M. Bintang, B. Priosoeryanto","doi":"10.29244/cb.6.2.3","DOIUrl":"https://doi.org/10.29244/cb.6.2.3","url":null,"abstract":"Green tea (Camellia sinensis) is one of tradisional plants that have potential as anticancer. Thesample used in this research was commercial green tea extract. The purpose of this study was to test theantiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract wasfractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and water fraction of greentea have Lethality Concentration 50 (LC50) value of more than 1000 ppm. Fractions of ethanol 70% and nhexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of phytochemicalscreening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screeningresults of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancercells MCM-B2 and normal cells Vero by tripan blue staining method. The highest MCM-B2 cell inhibitoryactivity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction,59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Verocells characterized by not inhibiting normal cell proliferation.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91017228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Bioactivity Test of Pectin from Musa balbisiana Peel Extracted using Various Acid Solvents 不同酸溶剂提取麝香果胶的特性及生物活性试验

Current cellular biochemistry Pub Date : 2020-11-18 DOI: 10.29244/cb.6.1.1

Husnawati Husnawati, Ika Yuni Astutik, L. Ambarsari

{"title":"Characterization and Bioactivity Test of Pectin from Musa balbisiana Peel Extracted using Various Acid Solvents","authors":"Husnawati Husnawati, Ika Yuni Astutik, L. Ambarsari","doi":"10.29244/cb.6.1.1","DOIUrl":"https://doi.org/10.29244/cb.6.1.1","url":null,"abstract":"Banana (Musaceaea) is one of the highest production fruits in Indonesia, which amounted to 7,162,685 tons in 2017. Banana peel waste produced can reach 40% of the total production of fresh bananas. Kepok banana peel contains a lot of nutrients and pectin. Pectin levels in banana peel ranged from 1.92% to 3.25% of dry weight. This study aims to characterize pectin from kepok banana peel (Musa balbisiana) extracted using nitric acid, citric acid, and HCl and screening potential of pectin bioactivity based on LC50 values. The highest pectin yield was obtained from extraction using pH 4 citrate buffer which was 3.68 ± 1.23%. In general, the characteristics of the best pectin from kepok banana peel are pectin extracted with HNO3 pH 4 with methoxyl degree 3.74 ± 0.34%, galacturonate content 87.64 ± 8.36%, and esterification degree 24.24 ± 0.098%. Based on the LC50 value the pectin extraction results can be classified into two, namely commercial pectin and pectin HCl pH 1.5 which is low bioactivity (LC50 > 100 ppm), and pH 4 HNO3 pectin and pH 4 citrate buffer pectin which has high bioactivity (LC50 ≤ 30 ppm). \u0000 \u0000Keywords: Cytotoxicity, galakturonat, kepok banana pectin, methoxyl","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81507170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro 花楸籽提取物生物活性成分鉴定及α-葡萄糖苷酶抑制活性研究

Current cellular biochemistry Pub Date : 2020-07-17 DOI: 10.29244/1-12

Oczhinvia Dwitasari, D. S. H. Seno, M. Safithri

{"title":"Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro","authors":"Oczhinvia Dwitasari, D. S. H. Seno, M. Safithri","doi":"10.29244/1-12","DOIUrl":"https://doi.org/10.29244/1-12","url":null,"abstract":"This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. \u0000Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79679925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae cordifolia Anredera叶片提取物的抑菌活性(十)抗肺炎链球菌

Current cellular biochemistry Pub Date : 2020-06-01 DOI: 10.29244/cb.7.1.1-9

Nuke Annisa Nasution, I. M. Artika, D. Safari

{"title":"Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae","authors":"Nuke Annisa Nasution, I. M. Artika, D. Safari","doi":"10.29244/cb.7.1.1-9","DOIUrl":"https://doi.org/10.29244/cb.7.1.1-9","url":null,"abstract":"Antibacterial resistance in Streptococcus pneumoniae has been increasing and is one of ongoing global concern. The need to find new antibacterial agents against Streptococcus pneumoniae is of paramount importance. Medicinal plants are prospective sources of antibacterial agents. The aims of the present study were to determine the activity of leaf extraxt of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. against Streptococcus pneumoniae. Leaves of Anredera cordifolia (Ten.) Steenis were extracted using 96% ethanol, while the leaves of Muntingia calabura L were extracted using 100% methanol. The leaf extracts of the two plants obtained were bioassayed for antibacterial activity against Streptococcus pneumoniae ATCC 49619 and a clinical isolate Streptococcus pneumoniae PU 067. Results showed that leaf extracts of both Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. have antibacterial activity in vitro against Streptococcus pneumoniae ATCC 49619 at crude extract concentrations of 25%, 50%, 75% and 100% (w/v). Both plants extracts showed strongest activity against S. pneumoniae ATCC 49619 at extract concentration of 75%. In addition, the extracts of both plants have inhibitory activity against growth of the clinical isolate Streptococcus pneumoniae PU 067. Both plant extracts showed strongest activity against S. pneumoniae PU 067 at extract concentration of 100%. Therefore, leaf extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. can potentially be used as a source of antibacterial agent for Streptococcus pneumoniae. \u0000Keywords: Antibacterial agent, Anredera cordifolia (Ten.) Steenis, Muntingia calabura L., Streptococcus pneumoniae.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"315 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80080572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Differences in Parts of Purslane Plants (Portulaca grandiflora Hook.) To Total Phenolic and Flavonoid Content and Antioxidant Activity 马齿苋属植物的部分差异(Portulaca grandflora Hook.)总酚和类黄酮含量及抗氧化活性

Current cellular biochemistry Pub Date : 2020-02-03 DOI: 10.29244/CB.7.1.10

Husnawati Husnawati, U. Safira

引用次数: 0

Toxicity Test of Roots, Stems and Leaves of Lemongrass (Cymbopogon nardus) 香茅根、茎、叶的毒性试验

Current cellular biochemistry Pub Date : 2019-12-23 DOI: 10.29244/cb.6.2.5

W. Nurcholis, Mustika Weni, R. Fitria, Najmah, Kornelia Rosvita Manek, Baharuddin Yusuf Habibie

{"title":"Toxicity Test of Roots, Stems and Leaves of Lemongrass (Cymbopogon nardus)","authors":"W. Nurcholis, Mustika Weni, R. Fitria, Najmah, Kornelia Rosvita Manek, Baharuddin Yusuf Habibie","doi":"10.29244/cb.6.2.5","DOIUrl":"https://doi.org/10.29244/cb.6.2.5","url":null,"abstract":"Lemongrass traditionally can be used as a mixture of water bath for rheumatic patients, antiseptic medicine, headache reducer, unbend the leech bite and overcome insect bite, but, the bioactivity potential of citronella lemongrass still remains unknown. The content of citronella lemongrass is known to have secondary metabolites such as flavanoid, phenolic, and terpenoid. The aim of this study is to determine the LC50 value (Lethality Concentration 50) in the root, stem, and leaves part of citronella lemongrass. Citronella lemongrass is extracted with maceration method using 96% ethanol solvent. Rendement value of leaf extract is greater than the value of stem and root extract, which is equal to 8.69% for stem extract, 3.73% for root extract and 9.70% for leaves extract. A toxicity test was performed by using the BSLT method, shrimp larvae were inserted into the test solution with each different solution concentration. LC50 values were obtained based on the calculation of percent mortality of shrimp larvae by using probit analysis. LC50 ethanol extract 96% of citronella lemongrass leaf is 67.841 μg/ml, citronella lemongrass stem is 31.604 μg/ml and citronella lemongrass root is 10.489 μg/ml. LC50 value shows that ethanol extract 96% of root, stem, and leaf of citronella lemongrass have no bioactivity potential due to the value of LC50 is greater than 1000 μg/ml. \u0000Keywords: BSLT, Cymbopogon nardus, cytotoxicity, LC50","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76230958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation Potential of Nanoparticles Moringa Leaves Extract as a Bioactive Candidate of Eco-Friendly Antifouling Paint 纳米辣木叶提取物作为环保防污漆生物活性候选物的评价潜力

Current cellular biochemistry Pub Date : 2019-08-08 DOI: 10.29244/CB.6.2.%P

L. Ambarsari, Riksa Nur Wahyuni, Agung Isnanto, Rifany Fairuz Aqilah

{"title":"Evaluation Potential of Nanoparticles Moringa Leaves Extract as a Bioactive Candidate of Eco-Friendly Antifouling Paint","authors":"L. Ambarsari, Riksa Nur Wahyuni, Agung Isnanto, Rifany Fairuz Aqilah","doi":"10.29244/CB.6.2.%P","DOIUrl":"https://doi.org/10.29244/CB.6.2.%P","url":null,"abstract":"Biofouling has become a serious problem for the maritime industry. To minimize the impact of biofouling are protected by antifouling coating made from Tributytlin - polishing copolymer paints (TBT - SPC paint) which are heavy metal groups. New alternative to replace the antifouling material is with the flavonoids in Moringa leaf extract. The objective of this research to analyze the potential of Moringa leaf ethanol extract, effectiveness and stability of Moringa leaves ethanol extract as antifouling and its potential to be a bioactive candidate in antifouling coating. Moringa leaves were extracted by maceration method using 96% ethanol and the yield was obtained about 9.32%. Flavonoid compounds were isolated using a liquid-liquid extraction method and obtained a yield of 60.11%. Flavonoid compounds are encapsulated in nanoparticles with chitosan. PSA analysis shows particle size nanoparticle moringa leaf extract of 31.26 nm. Activity of antifouling is determined by inhibition and degradation test using P. aeruginosa and B. subtilis. Moringa leaf nanoparticles and Moringa leaf flavonoid extract caused biofilm inhibition of 43.55% and 10.29% for P. aeruginosa, while for B. subtillis 25.85% and 82.58%. The result of biofilm degradation by Moringa leaf nanoparticles and Moringa leaf flavonoid extract was 6.9% and 2.2% for P. aeruginosa, while B. subtillis was 87.85% and 65.91%. \u0000 \u0000Keyword: antifouling coat, moringa leaves, nanoparticles. \u0000 \u0000 ","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76773628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of α-glucosidase Activity Seed Extract from Mahogany (Swietenia macrophylla King.) 红木种子提取物对α-葡萄糖苷酶活性的抑制作用

Current cellular biochemistry Pub Date : 2019-06-01 DOI: 10.29244/CB.6.1.5

W. Nurcholis, Rini Muthoharoh, Antonius Padua Ratu

引用次数: 0

Bioethanol Production from Tapioca-Waste as Potential Additive Fuel for LCGC (Low-Cost Green Car) 利用木薯渣生产生物乙醇作为低成本绿色汽车的潜在燃料添加剂

Current cellular biochemistry Pub Date : 2019-06-01 DOI: 10.29244/cb.6.1.4

D. Prastiwi, Novia Anggita, Yudha Putra Arishandy

{"title":"Bioethanol Production from Tapioca-Waste as Potential Additive Fuel for LCGC (Low-Cost Green Car)","authors":"D. Prastiwi, Novia Anggita, Yudha Putra Arishandy","doi":"10.29244/cb.6.1.4","DOIUrl":"https://doi.org/10.29244/cb.6.1.4","url":null,"abstract":"The majority style of people in transportation is 24% contribute to increasing premium demand as energy. Unfortunately, fossil fuel is not renewable and limited quantity. Its means, potential biomass of tapioca waste should be an alternative energy source to solve that problem. Today, the biosystem of bioethanol production needs to be optimized to maximize filtration and minimize the production cost. First, composition and time incubation needs to be optimized. The research found that to fermented 50 g waste on 200 mL water, we need 2 g of yeast, and the time of harvesting optimum is fourth days with 17% ethanol. Without acid hydrolyze, tapioca waste in yeast fermentation serves 2600 ppm glucose on the first day and increases significantly on the second day with 2964.77 ppm glucose. This concentration found by DNS (Dinitrosalicylic acid) method. Yeast is the subject that converts glucose on medium to be ethanol. Secondly, that medium distillate gets pure ethanol. Thirdly, this ethanol mix with premium in some concentration, include 0%, 10%, 20%, 30%, 40%, 50%. This variant would do test emission to understand the advantages of tapioca bioethanol compare to fossil fuel. The result of this research should be support government to enhance LCGC (Low-cost green car) program to achieve lower air pollutants, green energy resolution, and cleaner production in tapioca industry. \u0000 \u0000Key Word: bioethanol, emission, formulation, LCGC, tapioca,","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91140054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0