BMB Reports最新文献_第4页

JMJD4 promotes tumor progression via inhibition of the PDCD5-TP53 pathway. JMJD4 通过抑制 PDCD5-TP53 通路促进肿瘤进展。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Hyunsik Kim, Subhin Jang, Soo Yeon Lee, Jae-Hwan Kwon, Seunghee Byun, Jung-Yoon Yoo, Sungryul Yu, Soo-Yeon Park, Ho-Geun Yoon

{"title":"JMJD4 promotes tumor progression via inhibition of the PDCD5-TP53 pathway.","authors":"Hyunsik Kim, Subhin Jang, Soo Yeon Lee, Jae-Hwan Kwon, Seunghee Byun, Jung-Yoon Yoo, Sungryul Yu, Soo-Yeon Park, Ho-Geun Yoon","doi":"","DOIUrl":"","url":null,"abstract":"Programmed cell death 5 (PDCD5) regulates cell death and suppresses tumor progression. Since the stability and nuclear translocation of PDCD5 are regulated by TP53-dependent cell death stimuli, knowledge of the regulatory mechanism of PDCD5 function is required to better understand the TP53-signaling pathway. We identified Jumonji domain-containing protein 4 (JMJD4) to be a PDCD5-interacting protein using liquid chromatography- mass spectrometry (LC-MS). Interestingly, JMJD4 upregulates cell proliferation and chemo-resistance under genotoxic stress conditions by colony-formation assay and decreases TP53-related apoptotic genes (BAX, PUMA) by suppressing protein levels of PDCD5. Additionally, using the Cancer Genome Atlas and the Gene Expression Omnibus database to confirm the clinical correlation between JMJD4 and cancer patients, we verified that JMJD4 is associated with a poor prognosis in colon cancer and lung cancer patients. Therefore, this study demonstrates that JMJD4 directly interacts with PDCD5, regulates cancer cell death negatively, and could be a potential therapeutic target for cancer development. [BMB Reports 2025; 58(2): 64-69].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"64-69"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a prime editor with improved editing efficiency in Arabidopsis. 提高拟南芥编辑效率的主编辑器的研制。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Yeong Yeop Jeong, Cheljong Hong, Jun Hee Han, Sangsu Bae, Pil Joon Seo

引用次数: 0

Establishment and characterization of endometrial organoids from different placental types. 不同胎盘类型子宫内膜类器官的建立与表征。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Dong-Hyeok Kwon, Byeonghwi Lim, Sung-Yeon Lee, Sung-Ho Won, Goo Jang

{"title":"Establishment and characterization of endometrial organoids from different placental types.","authors":"Dong-Hyeok Kwon, Byeonghwi Lim, Sung-Yeon Lee, Sung-Ho Won, Goo Jang","doi":"","DOIUrl":"","url":null,"abstract":"Understanding molecular characteristics and metabolic processes of the mammalian endometrium is crucial for advancing biological research, particularly in veterinary obstetrics and pathology. This study established and analyzed organoids from endometrial epithelial stem cells of five mammals with different placental types: cows (cotyledonary), dogs and cats (zonary), pigs (diffuse), and rats (discoid). Organoids from these five species were maintained for over 13 passages, frozen, and thawed. Pathological analysis confirmed that they retained characteristics of their original tissues. Furthermore, integrative transcriptome analysis of organoids and tissues from the five species highlighted key pathways such as PI3K-Akt signaling and extracellular matrix-receptor interaction known to be crucial in cancer research. Although genes associated with vascular smooth muscle contraction were downregulated, these organoids exhibited significant activities of genes involved in hormone metabolism. In conclusion, our study achieved stable establishment of endometrial organoids from five mammals with different placental types, offering foundational data for organoid research. In the future, these organoids are suitable models for investigating uterine physiology and diseases and for developing potential therapies. [BMB Reports 2025; 58(2): 95-103].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"95-103"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-derived extracellular vesicles as nanocarriers for combination therapy enhancing paclitaxel-based regimens in breast cancer. 植物来源的细胞外囊泡作为纳米载体用于联合治疗增强紫杉醇为基础的乳腺癌方案。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Youngcheon Song, Hyunseok Kong, Soohwan Oh, Sang Bum Kim

{"title":"Plant-derived extracellular vesicles as nanocarriers for combination therapy enhancing paclitaxel-based regimens in breast cancer.","authors":"Youngcheon Song, Hyunseok Kong, Soohwan Oh, Sang Bum Kim","doi":"","DOIUrl":"","url":null,"abstract":"Breast cancer remains a leading cause of morbidity and mortality worldwide. Triple-negative breast cancer (TNBC) presents unique challenges owing to its aggressiveness and limited treatment options. Paclitaxel-based chemotherapy is widely used in breast cancer treatment. However, its efficacy is often limited by toxicity, multidrug resistance, and lack of targeted delivery. In response to these challenges, recent studies have focused on the use of extracellular vesicles (EVs), particularly plant-derived EVs, as innovative drug delivery systems capable of enhancing therapeutic outcomes and reducing adverse effects. Plant-derived EVs offer significant advantages owing to their biocompatibility, low immunogenicity, and scalability. They provide a natural platform for delivering chemotherapeutics such as paclitaxel and doxorubicin directly to tumor cells. This review explores the therapeutic potential of plant-derived EVs in breast cancer treatment, focusing on TNBC by examining their ability to improve drug stability, bioavailability, and selective targeting of cancer cells. Key studies on EVs derived from plants such as grapefruit, ginger, and tea leaves have demonstrated their capacity to deliver chemotherapeutic agents effectively while mitigating common side effects associated with conventional delivery methods. Although the use of plantderived EVs is still in early stages of research, findings suggest that that these nanocarriers can serve as transformative tools in oncology, providing a versatile and efficient platform for precise cancer treatment. This review highlights current landscape of research on plant-derived EVs, their application in breast cancer therapy, and future directions required to translate these findings into clinical practice. [BMB Reports 2025; 58(2): 53-63].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"53-63"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: Differential roles of N- and C-terminal LIR motifs in the catalytic activity and membrane targeting of RavZ and ATG4B proteins. 勘误：N端和C端LIR基团在RavZ和ATG4B蛋白的催化活性和膜靶向中的不同作用

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Sang-Won Park, Ju-Hui Park, Haneul Choi, Pureum Jeon, Seung-Hwan Lee, Won-Dong Shin, Hun-Joo Kim, Jin-A Lee, Deok-Jin Jang

{"title":"Erratum to: Differential roles of N- and C-terminal LIR motifs in the catalytic activity and membrane targeting of RavZ and ATG4B proteins.","authors":"Sang-Won Park, Ju-Hui Park, Haneul Choi, Pureum Jeon, Seung-Hwan Lee, Won-Dong Shin, Hun-Joo Kim, Jin-A Lee, Deok-Jin Jang","doi":"","DOIUrl":"","url":null,"abstract":"[Erratum to: BMB Reports 2024; 57(11): 497-502, PMID: 39384175, PMCID: PMC11608851] The BMB Reports would like to issue a correction to an article published in BMB Rep. 57(11): 497-502, titled \"Differential roles of N- and C-terminal LIR motifs in the catalytic activity and membrane targeting of RavZ and ATG4B proteins\". The original acknowledgment contained incorrect grant information. This has now been corrected at the authors' request as follows: The work was supported by the Science Research Center Program of the National Research Foundation NRF (2020R1A5A1019023); Neurological Disorder Research Program of the NRF (2020M3E5D9079911); Basic research program of the NRF (2023R1A2C2007082) to JAL. D.-J.J. was supported by the Basic Research Program of NRF (2022R1F1A1066552), and the NRF grant funded by the Korea government (MSIT) (RS-2023-00218515). Specifically, the grant number has been updated from [2023R1A2C2008092] to [2023R1A2C2007082]. The authors apologize for any inconvenience or confusion this error may have caused. The ACKNOWLEDGEMENTS section in the original PDF version has been updated accordingly.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":"58 2","pages":"104"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PGC1α is a key regulator of erastin-induced mitochondrial dysfunction during ferroptotic cell death. PGC1α是内皮素诱导的铁细胞死亡过程中线粒体功能障碍的关键调节因子。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Byeong Geun Seok, Eunhee Park, Young-Jun Park, Hyuk Nam Kwon, Su Wol Chung

{"title":"PGC1α is a key regulator of erastin-induced mitochondrial dysfunction during ferroptotic cell death.","authors":"Byeong Geun Seok, Eunhee Park, Young-Jun Park, Hyuk Nam Kwon, Su Wol Chung","doi":"","DOIUrl":"","url":null,"abstract":"A type of programmed cell death called ferroptosis is defined by increased iron-dependent lipid peroxidation. Mitochondria play a central role in iron metabolism. Mitochondrial defects include decreased cristae density, membrane rupture, and decreased mitochondrial membrane density, which occur as a result of ferroptosis. One of the important regulator of mitochondrial biogenesis is PGC1α. While recent studies have begun to explore the association between PGC1α and ferroptosis, the specific role of PGC1α in erastin-induced mitochondrial dysfunction during ferroptotic cell death has not been fully elucidated. In this study, we demonstrate for the first time that PGC1α is a key regulator of erastin-induced mitochondrial-dependent lipid peroxidation and dysfunction during ferroptosis in HT1080 fibrosarcoma cells. In this study, we examined PGC1α function in ferroptosis. Erastin, an inducer of ferroptosis, boosted the expression of PGC1α. Moreover, PGC1α down-regulation reduced erastin-induced ferroptosis. The most important biochemical feature of ferroptosis is the increase in iron ion (Fe2＋)-dependent lipid peroxide (LOOH) concentration. Mitochondrial-dependent lipid peroxidation was abolished by PGC1α downregulation. In addition, PGC1α was induced during mitochondrial dysfunction in erastin-induced ferroptosis. Mitochondrial membrane potential loss and mitochondrial ROS production associated with erastin-induced mitochondrial dysfunction were blocked by PGC1α inhibition. In addition, erastin-induced lipid peroxidation in HT1080 fibrosarcoma cells was regulated by PGC1α inhibitor. This phenomenon was also consistent in HT1080 cells transfected with PGC1α shRNA. Taken together, these results suggest that PGC1α is a key factor in erastin-induced mitochondrial-dependent lipid peroxidation and dysfunction during ferroptosis cell death. [BMB Reports 2025; 58(2): 89-92].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"89-94"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt5a exacerbates pathological bone features and trabecular bone loss in curdlan-injected SKG mice via osteoclast activation. Wnt5a通过破骨细胞激活，在注射胶原蛋白的SKG小鼠中加剧病理性骨特征和小梁骨丢失。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Min Whangbo, Eunae Ko, Dongju Kim, Chanhyeok Jeon, Hye-Ryeong Jo, Seung Hoon Lee, Jeehee Youn, Sungsin Jo, Tae-Hwan Kim

{"title":"Wnt5a exacerbates pathological bone features and trabecular bone loss in curdlan-injected SKG mice via osteoclast activation.","authors":"Min Whangbo, Eunae Ko, Dongju Kim, Chanhyeok Jeon, Hye-Ryeong Jo, Seung Hoon Lee, Jeehee Youn, Sungsin Jo, Tae-Hwan Kim","doi":"","DOIUrl":"","url":null,"abstract":"Many studies on osteoblasts have suggested that Wnt5a plays a crucial role in excessive osteoblast activity, which is responsible for ectopic new bone formation, but research on osteoclasts in ankylosing spondylitis (AS) remains relatively limited. This study aimed to explore whether Wnt5a influences osteoclastmediated bone resorption in curdlan-injected SKG mice, a model that mimics AS. Compared to the Vehicle group, the Wnt5a treatment group exhibited statistically higher clinical arthritis scores and increased hindpaw thickness values. Micro- computed tomography (microCT) analysis of hindpaws revealed a significant increase in inflamed and ectopic bone density in the Wnt5a-treated group compared to the Vehicle group. Histological examination also showed pronounced inflammation and structural bone damage in the bone marrow of ankles in the Wnt5a-treated group. Intriguingly, microCT analysis of the femur revealed that trabecular bone loss was markedly observed in the Wnt5a-treated group. Both the number of TRAP-positive osteoclasts and their activity were statistically greater in the Wnt5a-treated group compared to the Vehicle group. Serum markers of bone resorption, but not bone formation, were also significantly elevated in the Wnt5a-treated group. Notably, promotion of osteoclast differentiation by Wnt5a was inhibited following treatment with anti-Wnt5a. These findings suggest that targeting Wnt5a could be a promising strategy for mitigating pathological bone features in AS by modulating osteoclast activity. [BMB Reports 2025; 58(2): 75-81].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"75-81"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of histone modification in chromatin-mediated transcriptional repression in protozoan parasite Trichomonas vaginalis. 组蛋白修饰在原生寄生虫阴道毛滴虫染色质介导的转录抑制中的作用。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-02-01

Min-Ji Song, Mikyoung Kim, Jieun Seo, Heon-Woo Kwon, Chang Hoon Yang, Jung-Sik Joo, Yong-Joon Cho, Hyoung-Pyo Kim

{"title":"Role of histone modification in chromatin-mediated transcriptional repression in protozoan parasite Trichomonas vaginalis.","authors":"Min-Ji Song, Mikyoung Kim, Jieun Seo, Heon-Woo Kwon, Chang Hoon Yang, Jung-Sik Joo, Yong-Joon Cho, Hyoung-Pyo Kim","doi":"","DOIUrl":"","url":null,"abstract":"Trichomonas vaginalis is an extracellular flagellated protozoan responsible for trichomoniasis, one of the most prevalent nonviral sexually transmitted infections. To persist in its host, T. vaginalis employs sophisticated gene regulation mechanisms to adapt to hostile environmental conditions. Although transcriptional regulation is crucial for this adaptation, the underlying molecular mechanisms remain poorly understood. Epigenetic regulation, particularly histone modifications, has emerged as a key modulator of gene expression. A previous study demonstrated that histone modifications, H3K4me3 and H3K27ac, promote active transcription. However, the complete extent of epigenetic regulation in T. vaginalis remains unclear. The present study extended these findings by exploring the repressive role of two additional histone H3 modifications, H3K9me3 and H3K27me3. Genome-wide analysis revealed that these modifications negatively correlated with gene expression, affecting protein-coding and transposable element genes (TEGs). These findings offer new insights into the dual role of histone modifications in activating and repressing gene expression and provide a more comprehensive understanding of epigenetic regulation in T. vaginalis. This expanded knowledge may inform the development of novel therapeutic strategies targeting the epigenetic machinery of T. vaginalis. [BMB Reports 2025; 58(2): 82-88].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"82-88"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-molecule perspectives of CRISPR/Cas systems: target search, recognition, and cleavage. CRISPR/Cas 系统的单分子视角：目标搜索、识别和切割。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-01-01

Jeongmin Lee, Cherlhyun Jeong

引用次数: 0

Dynamics of nucleosomes and chromatin fibers revealed by single-molecule measurements. 单分子测量揭示核小体和染色质纤维的动力学。

IF 2.9 3区生物学

BMB Reports Pub Date : 2025-01-01

Sihyeong Nho, Hajin Kim

{"title":"Dynamics of nucleosomes and chromatin fibers revealed by single-molecule measurements.","authors":"Sihyeong Nho, Hajin Kim","doi":"","DOIUrl":"","url":null,"abstract":"The nucleosome is the fundamental structural unit of chromosome fibers. DNA wraps around a histone octamer to form a nucleosome while neighboring nucleosomes interact to form higher-order structures and fit gigabase-long DNAs into a small volume of the nucleus. Nucleosomes interrupt the access of transcription factors to a genomic region and provide regulatory controls of gene expression. Biochemical and physical cues stimulate wrapping-unwrapping and condensation-decondensation dynamics of nucleosomes and nucleosome arrays. Nucleosome dynamics and chromatin fiber organization are influenced by changes in the ionic background within the nucleus, post-translational modifications of histone proteins, and DNA sequence characteristics, such as histone-binding motifs and nucleosome spacing. Biochemical and biophysical measurements, along with in silico simulations, have been extensively used to study the regulatory effects on chromatin dynamics. In particular, single-molecule measurements have revealed novel mechanistic details of nucleosome and chromatin dynamics. This minireview elucidates recent findings on chromatin dynamics from these approaches. [BMB Reports 2025; 58(1): 24-32].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"24-32"},"PeriodicalIF":2.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0