Journal of stem cell research & therapy最新文献

{"title":"A novel point of care, automated, and closed system for processing stromal vascular fraction either with or without collagenase","authors":"pTodd K Malanp","doi":"10.4172/2157-7633-C2-035","DOIUrl":"https://doi.org/10.4172/2157-7633-C2-035","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Quest for Immortality: Introducing Metadicholandreg; a Novel Telomerase Activator","authors":"P. Raghavan","doi":"10.4172/2157-7633.1000446","DOIUrl":"https://doi.org/10.4172/2157-7633.1000446","url":null,"abstract":"Humans are keenly aware of their mortality. Given a limited time what we do with our life is a reflection of knowledge of our mortality. In 2009 the Nobel prize in medicine to Jack W Szostak, Elizabeth Blackburn, Carol W Greider for their work on Telomerase scientific research exploded in this area. Telomere and Telomerase protect chromosome ends the enzyme that maintains this. This activity of Telomerase is essential in cancer, aging and stem cells and achieving longer life spans. Telomerase is expressed in 85% of human cancer cell lines, but its enzymatic activity is not detectable in most human somatic cells which constitute the vast majority of the cells in the human body. There is a need for increased telomerase activity in stem cells for use in the treatment of therapies where there is an active role for telomerase. Umbilical Cord Blood (UCB) provides an attractive source of stem cells for research and therapeutic uses. Work present here characterizes the gene expression changes from Umbilical cord cells differentiate toward telomerase on treatment with Metadichol®. Metadichol® is a nanoemulsion of long-chain alcohols that is nontoxic. It is a mixture of long-chain alcohols derived from food. Since it expresses the Klotho gene which inhibits cancer cells it has fulfills the need for a safe telomerase inhibitor that reduces the risk of cancer. The work presented here is about the effect of Metadichol® on Telomerase expression profile in Umbilical cord cells. Our results using q-RT-PCR show increases of mRNA telomerase expression by Sixteen-fold at one picogram but down-regulates expression at higher concentrations of 100 pg, 1 ng, 100 ng and one microgram per ml concentration. Western blot studies showed expression of Telomerase protein which is slightly higher than control at one picogram, i.e., Telomerase protein expression continues at replacement level. This expression in vivo of mRNA is a new therapeutic pathway to mitigating diseases should be off of interest to researchers that is tantamount to making our bodies a drug factory. Since it is devoid of toxic effects, it can be directly tested on humans and is in use today as an immune boosting supplement. Metadichol® increases expression of Klotho an anti-aging gene expression in cancer cell lines by Four to Ten-fold, and Klotho gene has been documented to inhibits the growth of cancer cells. Metadichol® also inhibits TNF, ICAM1, CCL2, and BCAT1 which that is associated with proliferation in yeast and increased metastatic potential in human cancers. It paves the way for safe clinical testing and research and study of Telomerase biology and its use in humans.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"9 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70404343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robust, Efficient and Pure Induced Mesenchymal Stem Cells Generation frommRNA Induced Pluripotent Stem Cells in Suspension","authors":"R. Verma, Pornpun Saengmuang, Tanabodee Payuha, Julie D Mendoza, R. Narang, Naphapatsorn Bondee, Sergei Dmitrievs, P. Collier","doi":"10.35248/2157-7633/19.9.455","DOIUrl":"https://doi.org/10.35248/2157-7633/19.9.455","url":null,"abstract":"Mesenchymal Stem Cells (MSCs) are involved in many promising clinical trials tackling vastly complicated diseases. Many factors are determining the safety in these clinical trials such as the purity of tissue-derived MSCs cell population used in therapies. Also, the efficacy of the injected MSCs must be tested in-vitro, before application, through proliferation capacity and reproducibility over continuous passages. In addition to, the importance of choosing the right source of MSCs derivation for successful cellular therapy and transplantation. This study demonstrates robust generation of iMSCs from induced Pluripotent Stem Cells (iPSC) of healthy human donor (with full genetic test done prior) using non-integrative (mRNA) method. This conversion method comprises (i) differentiating a population of iPSCs in suspension without iMatrix, (ii) passaging the cells differentiated in step (i) in the presence of a conditioned MSC medium for a time and under conditions sufficient to produce iMSC in culture for long term with no sign of epigenetic memory. Analysis of Pluripotent markers expression (Oct-4, SSEA-4, Sox-2, Tra-1-60) was confirmed by flow cytometry and Immunocytochemistry through Fluorescence microscope visual assessment. No teratoma was developed by in-vivo injection of the iMSC population in male hamsters, confirming the transformed purity of iMSCs and the immunemodulating property in culture without iPSC respectively. For cell cycle and senescence studies, pure in-vitro iMSCs were tested using flow cytometry using CD73, 90 and 105 expression analysis and compared with UC-MSC. Later, iMSCs demonstrated tri-differentiation of chondrocytes, osteocytes and adipocytes relative to UC-MSCs, which could make it possible to address the drawbacks of using adult MSCs and thus provide a valuable tool for future use in various clinical settings.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"9 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69975742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of glucose on the expression of CD24 marker in Umbilical-cord derived Mesenchymal Stem Cells","authors":"Rithika Rajendran, F. Suman, A. Punnoose, S. Kuruvilla, S. Krishnakumar","doi":"10.4172/2157-7633.1000444","DOIUrl":"https://doi.org/10.4172/2157-7633.1000444","url":null,"abstract":"A cluster of Differentiation 24 (CD24) is considered as a marker of pancreatic progenitor cells in the mouse. In human embryonic stem cells, CD24+ cells expressed Pancreatic Duodenal Homeobox 1 (PDX1) and insulin after 1-week culture. PDX1 is a transcription factor that is essential for pancreatic differentiation and beta cell maturation. These cells could provide a source for beta cell replacement. To define the proper culture conditions for Mesenchymal Stem Cells (MSCs) to differentiate into islet cells, the CD24 expression at the MSC level with various glucose concentrations may be helpful. This research aims to study the expression of CD24 in MSCs derived from umbilical cord Wharton’s jelly with varying concentrations of glucose in vitro. MSCs were isolated by enzymatic digestion using standard protocols from umbilical cord Wharton’s jelly with ethical approval. The MSCs were characterized by standard criteria. Pancreatic progenitor marker CD24 expression was analyzed at serial passages from passage 0 to passage 4. Passage 1 with maximum expression and passage 3 with lesser expression were chosen for culture with high glucose medium of 2X (11.2 mmol), 4X (25 mmol) and 8X glucose concentrations (50 mmol) for 4 weeks. Flow cytometry for CD24 was performed for all the cell populations. A graded dose-dependent increase in CD24 expression was observed in passage 1. The expression of the marker was present but less in graded glucose with cells from passage 3. The possibility of CD24 expression being stimulated by glucose is evident though it warrants further research. This study suggests that passage 1 MSCs with high glucose medium may be selected for further research on pancreatic islet development. Effect of Glucose on the Expression of CD24 Marker in Umbilical CordDerived Mesenchymal Stem Cells Rithika Rajendran, Febe Renjitha Suman*, Alan Mathew Punnoose, Sarah Kuruvilla and S Krishnakumar Department of Pathology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai, India *Corresponding author: Febe Renjitha Suman, Department of Pathology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai, India, Tel: +919994081470; E-mail: johnjennerresearch@gmail.com Received December 27, 2018; Accepted January 25, 2019; Published January 30, 2019 Citation: Rajendran R, Suman FR, Punnoose AM, Kuruvilla S, Krishnakumar S (2019) Effect of Glucose on the Expression of CD24 Marker in Umbilical CordDerived Mesenchymal Stem Cells. Stem Cell Res Ther 9: 444. doi: 10.4172/21577633.1000444 Copyright: © 2019 Rajendran R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation Impacts Cell Functionality of Long Term Expanded Adipose-Derived Stem Cells","authors":"Alice Othmani, Sabrina Rouam, Anass Abbad, Chaimaa Erraoui, Sara Harriba, H. Boukind, J. Nourlil, G. Malka, L. Mazini","doi":"10.4172/2157-7633.1000445","DOIUrl":"https://doi.org/10.4172/2157-7633.1000445","url":null,"abstract":"Objective: Adipose-Derived Stem Cells (ADSCs) are less immunogenic cells and have a heterogenic cytokine secretion profile making them a better candidate for cell-based immunotherapy. Even innately or stimulated, Interleukin-6 (IL-6) and Toll-like Receptor 2 (TLR2) secreted by ADSC were modulated and led to different inflammatory mechanism pathways through different inflammatory factors secretion. These properties can be very useful in the treatment of inflammation-associated chronic diseases. To be used, ex-vivo ADSC expansion is a critical issue prior to transplantation or cryopreservation. Functional cell changes have been reported during culture expansion being susceptible to interact with freezing/thawing effects leading to doubt on cell therapeutic outcomes. The aim of this study is to identify the effect of freezing/thawing at the different time point of expansion culture on IL-6 and TLR2 secretion.Methods: ADSC were collected from young female donors, expanded in culture and cryopreserved in Foetal Bovine Serum (FBS) and Dimethylsulfoxide (DMSO) after each passage for 6 months to a year. ADSC was then tested for proliferation, clonogenicity, cytokine gene expression and assessment before cryopreservation (fresh) and after thawing and cultured until confluence (frozen/thawed). ADSC preserved at Passage 0 (P0) were thawed and tested after confluence at P1.Results: Cryopreserved ADSC as P1 resulted in increased clonogenicity, total RNA and protein secretion compared to the fresh ones. Relative Quantification (RQ) and cytokine assessment of IL-6, IL-10, Tumor Necrosis Factor (TNF)-α and TLR2 revealed a moderate up-regulation of TLR2 while significantly higher IL-6 secretion levels were observed in long term expanded and cryopreserved ADSC.Conclusion: Our results suggested that cryopreserved ADSC long term expanded in culture were functionally different and might have impaired immunosuppressive properties through modulation of the inflammatory responses by IL-6 and TLR2 activation.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Year Results of Subretinal Mesenchymal Stem Cell Implantation in Severe Retinitis Pigmentosa","authors":"A. Öner, Z. Gönen, D. Sevim, N. Kahraman, M. Çetin, Y. Ozkul","doi":"10.35248/2155-6156.19.9.454","DOIUrl":"https://doi.org/10.35248/2155-6156.19.9.454","url":null,"abstract":"This study includes one-year results of 14 patients with severe Retinitis Pigmentosa (RP) who had subretinal mesenchymal stem cell (ADMSC) implantation. The highest Visual Acuity (VA) in the study was 20/2000 and 7 of the patients had severe VA loss. The patients received subretinal ADMSCs after total vitrectomy. We observed no systemic complications. There were no ocular complications in 8 patients. Choroidal Neovascular Membrane (CNM) developed in one of the patients and intravitreal anti-VEGF injection was performed. The first six patients had Epiretinal Membrane (ERM) with peripheral tractional retinal detachment and received second vitrectomy. One of the patients experienced mild band keratopathy six months after the treatment and another patient had retrolental fibrous tissue at 1-year follow-up examination. Four patients showed VA gain during the first year. Subretinal implantation of ADMSCs may have some adverse effects and the patients should be followed up carefully. This study clarifies the side effects of the therapy which would enlighten future studies. Further studies with higher number of patients will be necessary to optimize the surgical procedure and to determine the benefits of this therapy.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"9 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metadichol® and CD33 expression in Umbilical Cord Cells","authors":"P. Raghavan","doi":"10.4172/2157-7633.1000443","DOIUrl":"https://doi.org/10.4172/2157-7633.1000443","url":null,"abstract":"Umbilical cord blood has found use in the clinic for more than 40 years in hematopoietic stem cell transplantation therapies to treat patients with bone marrow diseases or to reconstitute the bone of those cancer patients who had to have theirs wiped out to cure their leukemia or lymphoma. A feature is the presence of CD34 antigen in of hematopoietic stem and progenitor cells. These cells can differentiate and are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system and erythrocytes), and lymphoid (T cells, B cells, and NK cells) lineages. This study describes increased CD34 gene expression in Umbilical Cord (UC) cells upon treatment with Metadichol which is an inverse agonist of AHR (Aryl Hydrocarbon Receptor). UC cells were subjected to treatment at one picogram, 100 picograms, 1 nanogram, 100 nanograms and 1 microgram per ml of Metadichol for 72 hrs. Cells treated at 1ng have shown the highest increase in expression of CD34 compared to untreated Control. The cells treated with 1 pg, 100 picogram/ml demonstrated the multiplicity of CD34 expression as indicated by peak shift compared to treatment with 1ng, 100 ng, and 1 μg","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Methyl Mercury on the Cerebellar Cortex of Rats and the Possible Neuroprotective Role of Mesenchymal Stem Cells Conditioned Medium. Histological and Immunohistochemical Study","authors":"N. El-Azab, Abeer M El-Mahalaway, D. Sabry","doi":"10.4172/2572-4126.1000430","DOIUrl":"https://doi.org/10.4172/2572-4126.1000430","url":null,"abstract":"BACKGROUND: Methyl mercury (Me Hg) is an environmental toxin associated with many serious neurological disorders. Conditioned Medium (CM) derived from Mesenchymal Stem Cells (MSCs) is a novel promising approach for the treatment of nervous system damage and various diseases. OBJECTIVE: The aim of this research is to assess the consequence of Me Hg on the rats’ cerebellar cortex and the potential neuroprotective effect of MSCs – CM. MATERIALS AND METHODS: Forty adult male albino rats were divided into four groups: Group I: control rats; Group II: Me Hg chloride treated rats; Group III: Me Hg chloride and DMEM treated rats; Group IV: CM treated rats after injection with Me Hg chloride. Cerebellar specimens were taken and handled for histological and immunohistochemical techniques. Morphometrical studies and statistical analysis were performed. RESULTS: Groups II and III showed various changes such as neuronal degeneration and apoptosis. The mean number of Purkinje cells was significantly decreased (P<0.01), while Glial Fibrillary Acidic Protein (GFAP) immunostaining was significantly increased (P<0.01) in the neuroglial cells. Ultrastructural examination showed thinning and apparent decrease in the number of myelinated nerve axons. Shrunken Purkinje cells were observed with irregular nuclei, heterogenous cytoplasm, and disrupted mitochondria. Group IV showed improvement of the histological and electron microscopic changes defined in groups II and III. CONCLUSION: Me Hg exposure led to degenerative changes on cerebellar cortex. MSCs – CM is a very promising approach and has neuroprotective effects.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2018-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2572-4126.1000430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42317005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory Training Guidelines for Clinicians Undertaking Stem Cell Therapy","authors":"Rachel A. Shparberg, Natasha Braunsteiner, E. R. Vickers","doi":"10.4172/2157-7633.1000427","DOIUrl":"https://doi.org/10.4172/2157-7633.1000427","url":null,"abstract":"Stem cell-based regenerative therapies are an exciting and emerging field in medicine and dentistry. Since the 1960s, stem cell therapies have been successfully performed and approved in the form of bone marrow transplants and more recently, in skin and corneal grafting. However, the field of regenerative cell therapy has somewhat come to a halt due to safety, ethical and legal concerns associated with medical and scientific practices. In Australia, stem cell therapies, besides those mentioned above, are permitted provided that they are autologous in nature and that the procedure is performed by or under the supervision of a registered medical practitioner in a single treatment. However, the medical practitioner requires no formal stem cell training. We have identified this deficiency in optimal patient care and propose that education in the safe, legal and ethical delivery of stem cell treatments be part of a mandatory training course for medical/dental practitioners and the scientific team prior to the clinical use of stem cell therapies in Australia, and arguably, on a global scale. This involves the scientific and medical team having a sound understanding of the biology of stem cells, their appropriate applications and basic validation and laboratory techniques in order to provide effective and safe treatment for improved patient outcomes. As such, this article aims to propose a frame-work of scientific guidelines for dental and medical practitioners to undertake stem cell therapies.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45282051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells to Osteoblast Cell in Early, Middle and Late Passages","authors":"R. Rilianawati, Jennifer Bratakencana, Ago Harlim","doi":"10.4172/2157-7633.1000426","DOIUrl":"https://doi.org/10.4172/2157-7633.1000426","url":null,"abstract":"Stem cell-based regenerative therapies are an exciting and emerging field in medicine and dentistry. Since the 1960s, stem cell therapies have been successfully performed and approved in the form of bone marrow transplants and more recently, in skin and corneal grafting. However, the field of regenerative cell therapy has somewhat come to a halt due to safety, ethical and legal concerns associated with medical and scientific practices. In Australia, stem cell therapies, besides those mentioned above, are permitted provided that they are autologous in nature and that the procedure is performed by or under the supervision of a registered medical practitioner in a single treatment. However, the medical practitioner requires no formal stem cell training. We have identified this deficiency in optimal patient care and propose that education in the safe, legal and ethical delivery of stem cell treatments be part of a mandatory training course for medical/dental practitioners and the scientific team prior to the clinical use of stem cell therapies in Australia, and arguably, on a global scale. This involves the scientific and medical team having a sound understanding of the biology of stem cells, their appropriate applications and basic validation and laboratory techniques in order to provide effective and safe treatment for improved patient outcomes. As such, this article aims to propose a frame-work of scientific guidelines for dental and medical practitioners to undertake stem cell therapies.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41632499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}