Effect of glucose on the expression of CD24 marker in Umbilical-cord derived Mesenchymal Stem Cells

Abstract

A cluster of Differentiation 24 (CD24) is considered as a marker of pancreatic progenitor cells in the mouse. In human embryonic stem cells, CD24+ cells expressed Pancreatic Duodenal Homeobox 1 (PDX1) and insulin after 1-week culture. PDX1 is a transcription factor that is essential for pancreatic differentiation and beta cell maturation. These cells could provide a source for beta cell replacement. To define the proper culture conditions for Mesenchymal Stem Cells (MSCs) to differentiate into islet cells, the CD24 expression at the MSC level with various glucose concentrations may be helpful. This research aims to study the expression of CD24 in MSCs derived from umbilical cord Wharton’s jelly with varying concentrations of glucose in vitro. MSCs were isolated by enzymatic digestion using standard protocols from umbilical cord Wharton’s jelly with ethical approval. The MSCs were characterized by standard criteria. Pancreatic progenitor marker CD24 expression was analyzed at serial passages from passage 0 to passage 4. Passage 1 with maximum expression and passage 3 with lesser expression were chosen for culture with high glucose medium of 2X (11.2 mmol), 4X (25 mmol) and 8X glucose concentrations (50 mmol) for 4 weeks. Flow cytometry for CD24 was performed for all the cell populations. A graded dose-dependent increase in CD24 expression was observed in passage 1. The expression of the marker was present but less in graded glucose with cells from passage 3. The possibility of CD24 expression being stimulated by glucose is evident though it warrants further research. This study suggests that passage 1 MSCs with high glucose medium may be selected for further research on pancreatic islet development. Effect of Glucose on the Expression of CD24 Marker in Umbilical CordDerived Mesenchymal Stem Cells Rithika Rajendran, Febe Renjitha Suman*, Alan Mathew Punnoose, Sarah Kuruvilla and S Krishnakumar Department of Pathology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai, India *Corresponding author: Febe Renjitha Suman, Department of Pathology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai, India, Tel: +919994081470; E-mail: johnjennerresearch@gmail.com Received December 27, 2018; Accepted January 25, 2019; Published January 30, 2019 Citation: Rajendran R, Suman FR, Punnoose AM, Kuruvilla S, Krishnakumar S (2019) Effect of Glucose on the Expression of CD24 Marker in Umbilical CordDerived Mesenchymal Stem Cells. Stem Cell Res Ther 9: 444. doi: 10.4172/21577633.1000444 Copyright: © 2019 Rajendran R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.