Journal of stem cell research & therapy最新文献

{"title":"Induction of immune tolerance towards allogeneic cells using fetal directed placental injection in a murine model","authors":"Yukiko Shimazu, M. Endo, K. Tamai, Kei Takahashi, T. Miyoshi, HiroshiHosoda, A. Flake, T. Kimura, J. Yoshimatsu","doi":"10.35248/2157-7633/19.9.456","DOIUrl":"https://doi.org/10.35248/2157-7633/19.9.456","url":null,"abstract":"Objective: In utero exposure to foreign antigens prior to the development of the immune system induces immune tolerance. We aimed to induce immune tolerance towards allogeneic cells by early gestational transplacental injection under ultrasound guidance in a murine model. Methods: Bone marrow cells from C57BL/6-Green Fluorescence Protein transgenic mice were transplanted into the placenta of Balb/c fetal mice at 11-day of gestation under ultrasound guidance. Each fetus was injected with 2 × 105 cells/2.5 μl. After birth, we evaluated the immune response against allogeneic donor cells. Results: The birth survival rate was 21.2% for allogeneic mice. Survival of the donor skin graft was 75% and successful in mice injected with fetal transplacental cells, whereas the transplanted allogeneic skin was all rejected within 4 weeks in control naive mice (p=0.007). Cytotoxic immune reactivity against the allogeneic cells was suppressed according to the ELISPOT assay (p=0.002). Conclusion: We showed that early transplacental allogeneic cell injection can induce donor-specific tolerance sufficient to allow tissue graft.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"10 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69975772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell Therapy Target EMT Process in Glioma","authors":"Yu-bao Lu, Tian-jiao Sun, Jia-yu Zhao, Jianghong Wang, Feng Miao, Shi-xin Wang","doi":"10.35248/2157-7633/20.10.457","DOIUrl":"https://doi.org/10.35248/2157-7633/20.10.457","url":null,"abstract":"Glioma is one of the most common tumors in the nervous system. It's initiation, migration and the multipotency are effected by cancer stem cell ’s transition. A previous study implies that through change how cancer stem cell performs can affect the malignant differentiation of the tumor. We found some treatments target the regulatory pathways related to Epithelial To Mesenchymal Transition (EMT) of the tumor. In this review, we discuss the transition factor of EMT and three specific pathways which affect EMT of cancer stem cell to affect tumor development. Suggesting that targeting EMT of cancer stem cells can be a feasible pathway of cancer treatment.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"10 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69975340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MSC Transplantation in Eight Severe COVID-19 Patients: Can Cytokine Storm Be Reversed?","authors":"N. Erçelen, B. Bilgili, B. Monteleone, F. Gül, G. Gulay, Nagihan Alpaydin, Ozan Demir, Murat Şimşek, D. Turan, Omer Karadeniz, Elif Karadeniz, Nagihan Batıgun, I. Cinel","doi":"10.35248/2157-7633/20.10.460","DOIUrl":"https://doi.org/10.35248/2157-7633/20.10.460","url":null,"abstract":"The COVID-19 disease is a global pandemic, with the first case diagnosed in December 2019, as reported by World Health Organization (WHO). In 17% of patients, COVID-19 causes severe Acute Respiratory Distress Syndrome (ARDS) due to release of large amounts of pro-inflammatory cytokines and chemokines in the lungs. In a retrospective observational study from Milan, 9% of the people, who tested positive for COVID-19, needed ICU care with respiratory support. The demand for ICU beds and health care personnel brought significant overload to sustain the care of these patients. Search for effective therapies is underway. However, the result of severe infection of COVID-19 still leads to the inevitable fatalities with the current available therapies. Mesenchymal Stem Cells (MSCs) have been isolated about 30 years ago (Supplementary Data 1). There are over 5,000 articles published on MSCs. Moreover, anti-inflammatory and immunemodulatory properties of MSCs have been well studied. Exogenously administered MSCs are medicinal. They generate positive therapeutic outcomes by secreting bioactive factors that exhibit immunomodulatory, and regenerative effects by fabricating, and secreting antibiotic proteins, where they hone in on sites of injury or disease. Hence, Arnold Caplan has proposed recently to change the name of MSCs to Medicinal Signaling Cells. As MSCs arise from pericytes, they can be isolated from a variety of vascularized tissues. Each separate tissue-specific stem cell interacts with its underlying vascular endothelial cells, and adjacent specific pericyte/MSC “Universal Stem Cell Niche” (pMSCs). Each specific pMSCs have both pMSCs common, and unique chemical, and functional features. Meanwhile, the major therapeutic role of pMSCs in vivo at various sites of disease or injury are very similar when comparing these different pMSCs. Over the past decade, the emphasis has shifted toward harnessing the pMSCs’ ability to produce factors and cytokines that stimulate innate tissue repair, modulate inflammation, and immune responses. MSCs express function on Toll-Like Receptors (TLRs). Triggering different TLRs, depending on exposure times promote either pro- or anti-inflammatory function in MSCs. Pre-clinical studies demonstrated that the majority of infused MSCs initially distributed in the lungs. Subsequent studies showed improved pulmonary functions beginning shortly after administration with no evidence of pulmonary safety risk. These studies indicated the local beneficial MSCs-mediated effect on pulmonary airways . A recent pilot study from China explored the therapeutic outcomes of MSC transplantation in seven poor prognoses COVID-19 patients with pneumonia. The results revealed that MSC transplantation was safe and effective treatment option. The peripheral lymphocytes increased after the treatment, and the overactivated cytokine-secreting immune cells disappeared in 3-6 days. A group of regulatory DC cell population dramatically increased. Meanw","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"10 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69975473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amniotic membrane mapping discloses novel promising features of amniotic membrane epithelial cells for regenerative medicine purposes","authors":"R. Pietro","doi":"10.35841/BIOMEDICAL.4.3.11-11","DOIUrl":"https://doi.org/10.35841/BIOMEDICAL.4.3.11-11","url":null,"abstract":"The amniotic membrane (AM) is the innermost part of the placenta, in direct contact with the amniotic fluid. In recent years the interest toward placenta stem cells has been increasingly growing, due in part to the absence of any ethical issues concerning their isolation. At present, two main stem cells populations have been identified in AM: amniotic epithelial cells (AECs) and amniotic mesenchymal stromal cells (AMSCs). Albeit AM is an excellent source of cells for regenerative medicine, additionally due to its immune-modulatory properties and low immunogenicity, only a few papers have studied its sub-regions. Thus, our focus was to map the human AM under physiological conditions to identify possible differences in morpho-functional features and regenerative capacity of its components. Human term placentas were amassed from salubrious women after vaginal distribution or caesarean section at Fondazione Poliambulanza-Istituto Ospedaliero of Brescia, University Hospital of Cagliari and SS. Annunziata Hospital of Chieti. Samples of AM were isolated from four different regions according to their position relative to umbilical cord (central, intermediate, peripheral, reflected). By designates of immunohistochemistry, morphometry, flow cytometry, electron microscopy, CFU assays, RT-PCR and AECs in vitro differentiation we demonstrated the esse of different morpho-functional features in the different regions of AM, highlighting that AECs are a heterogeneous cell population. This should be considered to increment efficiency of amniotic membrane application within a therapeutic context.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"10 1","pages":"11-11"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70032976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Secretoma of Adipose-Derived Stem Cells from Ischemic Patients","authors":"I. Uçkay, M. Baquié, Sébastien Mosser, Marie Priscille Herve, Pascale Bruyere Cerdan, P. R. Lombard, C. Modoux, Lasta Kocjancic Curty, E. Rüegg, Dimitrios Stafylakis, Sten Ilmarjv, N. Brembilla, K. Krause, Olivier Preynat Seauve","doi":"10.4172/2157-7633.1000452","DOIUrl":"https://doi.org/10.4172/2157-7633.1000452","url":null,"abstract":"Patients with terminal ischemia often reveal chronic limb and foot ulcers with subsequent risk of infection and/or amputation. Adipose-derived Stem Cells (ASC) may secrete angiogenic and regenerative factors. The autologous transplantation of such cells is considered to be an attractive therapeutic strategy, but their functional properties of ASC are influenced by many biochemical and biophysical stimuli of the microenvironment. Thus, patient-derived ASC might not be functionally competent. To study ASCs in ischemic disease, we have generated ASC lines from fat tissue of twelve ischemic patients. Lines were characterized for cell surface phenotype, multipotent capacities, and production of factors involved in wound healing. We succeeded to amplify ASC lines from all twelve patients and confirmed an ASC identity by their ability to: (i) adhere and grow on a plastic surface in standard culture conditions; (ii) express an ASC expression profile; (iii) differentiate in vitro into adipocytes, osteoblasts, and chondroblasts. Full transcriptome analysis of four selected lines showed a gene expression profile compatible with healing properties including all of the functional families involved in the wound healing process: extracellular matrix proteins, cell growth factors, pro-inflammatory cytokines, angiogenic factors, and matrix remodeling proteins. Our pilot study confirms that high-quality adipose stem cells can be easily derived from ischemic patients. Their transcriptome and secretome show a regenerative profile which makes them promising candidates for autologous therapy of chronic ulcers.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"9 1","pages":"1000452"},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49644716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metadicholandreg; a Novel Sialidase Inhibitor","authors":"R. Pr, S. Yogisha, S. Anand, Purushotham Gv","doi":"10.4172/2157-7633.1000449","DOIUrl":"https://doi.org/10.4172/2157-7633.1000449","url":null,"abstract":"Humans face a constant threat from pathogens like influenza varieties H1N1, H5N1, and others and there is a need to prevent these from epidemics. The pathogens depend on successful colonization of the host in order to reproduce and multiply. Sialidases are known as neuraminidases are a group of enzymes, the most abundant of these being the exo-sialidases that can catalyze the cleavage of sialic acids from carbohydrates, glycoproteins or glycolipids. Sialidases have been thoroughly studied since their discovery 75 years ago and their occurrence in bacteria and viruses is widespread. They are found in diverse virus families and bacteria and other microbes. Moreover, sialic acids serve as a receptor for various pathogens. This allows bacteria like H1N1 or other influenza viruses, to enter the host cell. There is a need to block sialidases as they release sialic acid that serves as nutrition for the microbes and as well allows them to bind and invade the host cell where they can proliferate. This makes sialidases an interesting target to control pathogenic activity. \u0000Metadichol® is nanoemulsion of long-chain lipid alcohols derived from food ingredients. In rats, it has an LD50 of 5000 mg/kilo and its ingredients are present in many foods we consume on a daily basis. It has antiviral and antibacterial and anti-parasitic properties. We studied inhibition of Sialidases by inducing it with Lipopolysaccharide (LPS) using THP1 cells. Metadichol showed inhibition at 1 picogram per ml to 1 nanogram per/ml. Compared to Prednisone. It is 100 times more active. Previous studies on Metadichol® showed that it is toxic to cancer cells at higher concentrations. \u0000Since it is safer, it has the potential of being directly tested on humans without side effects and could have a potential role in mitigating the pathogens that a burden on the Public health system.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"9 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2019-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47735558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foxd1-Dependent and Independent Pathways for Reprogramming from Fibroblasts to Induced Pluripotent Stem Cells or Cardiomyocytes","authors":"S. Nakao, Tasuku Tsukamoto, D. Ihara, Yukihiro Harada, Tomoe Ueyama, T. Ishida, Chihiro Tokunaga, Tomomi Akama, Takahiro Sogo, Teruhisa Kawamura","doi":"10.4172/2157-7633.1000448","DOIUrl":"https://doi.org/10.4172/2157-7633.1000448","url":null,"abstract":"Induced Pluripotent Stem Cells (iPSCs) can differentiate into any cell type. Cardiomyogenesis from iPSCs is useful for clinical application in myocardial regeneration. However, the efficiency and duration of producing iPSCs and iPSC-derived cardiomyocytes must be improved. We previously demonstrated that a surface marker profile of Sca1-CD34- or Foxd1+ during the reprogramming process is a predictor of successful iPSC formation. Here, we examine the correlation of feasibility as iPSC predictors between Sca1-CD34- and Foxd1+ cell populations, and their possibility as predictors for cardiomyocyte transdifferentiation. The fate-tracing analysis revealed that most iPSC colonies were formed from GFP-positive cells in which Foxd1 was transactivated in the middle-to-late phase of the reprogramming process. In addition, GFP expression was observed mainly in the Sca1-CD34- cell population. Thus, Foxd1+ could be an indicator of successful reprogramming to iPSCs mainly derived from Sca1-CD34- cells. As for cardiac transdifferentiation, reprogramming cells were sorted based on the expression pattern of Sca1 and CD34, resulting in a higher incidence of beating cell aggregates derived from theSca1+CD34+ population, which expresses less Foxd1 promoter-driven GFP and contained very few undifferentiated iPSCs. Moreover, the cardiomyocyte marker α-actinin only partially co-localized with GFP expression in the aggregates derived from Sca1+CD34+ or Sca1-CD34- cells. Therefore,Sca1+CD34+ could be a better cell source for Foxd1-independent cardiomyocyte creation despite the failed reprogramming cell population.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"9 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2019-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43308276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New horizon in private cord blood banking","authors":"pFalk HeinrichsohnLalit Jaiswalp","doi":"10.4172/2157-7633-C4-040","DOIUrl":"https://doi.org/10.4172/2157-7633-C4-040","url":null,"abstract":"Introduction: As of now, two-dimensional (2D) stages in which level monolayer cells are refined is as yet the most regularly utilized for the exploration of cell-based measures. The 2D cell culture frameworks are simple, helpful, financially savvy, and broadly utilized. In any case, different downsides and impediments are still of concern. The main downside of a 2D cell culture frameworks is that a genuine three-dimensional (3D) condition in which malignancy cells dwell in vivo isn't precisely mirrored. The unimportant 2D condition may give deluding results with respect to the anticipated reactions of preclinical screening culture the preliminaries. each of anticancer specialists little","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of bromodomain extra terminal proteins in cellular reprogramming","authors":"pKejin Hup","doi":"10.4172/2157-7633-C6-047","DOIUrl":"https://doi.org/10.4172/2157-7633-C6-047","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42933941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human umbilical cord mesenchymal stem cells inhibits acute myeloid leukemic cell line (K562) in vitro","authors":"pMuneerah A Huwaikem Farid Ahmed Mohammed AlQahtani, Kalamegam Gauthamanp","doi":"10.4172/2157-7633-C5-045","DOIUrl":"https://doi.org/10.4172/2157-7633-C5-045","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}