Biological Procedures Online最新文献

{"title":"Integrated Genomic and Single-Cell Analysis Identifies Notch1 as a Metastasis Suppressor in Nasopharyngeal Carcinoma Targeted by the AKT/Smad3 Axis.","authors":"Jingjing Zuo, Shiyong Huang, Mengyuan Dai, Fen Li, Ting Huang, Lei Wang, Chenglin Qi, Zezhang Tao, Linfeng Ye","doi":"10.1186/s12575-026-00341-5","DOIUrl":"https://doi.org/10.1186/s12575-026-00341-5","url":null,"abstract":"<p><strong>Background: </strong>Notch1 exhibits a high mutation rate in head and neck squamous cell carcinoma (HNSCC), but its functional role in nasopharyngeal carcinoma (NPC) invasion and metastasis remains incompletely understood. This study systematically investigates the function and molecular mechanisms of Notch1 as a metastatic suppressor in NPC.</p><p><strong>Methods: </strong>Notch1 expression levels were assessed in NPC tissue microarrays using immunohistochemistry, and correlations with tumor differentiation and metastasis were analyzed. TCGA databases were mined to explore mutation rates and sample clustering based on EMT-related gene signatures. Integrated analysis of TCGA and GEO datasets identified Notch1-associated genes, enabling molecular classification of patients. Additionally, scRNA-seq of NPC clinical samples was performed to validate the association between Notch1, stemness, and EMT features in the tumor microenvironment. The biological functions of Notch1 were further examined in vitro using proliferation (CCK-8, colony formation), migration and invasion (Transwell, wound healing) assays, as well as in vivo xenograft and metastatic models. Molecular mechanisms were probed by examining EMT markers, Smad3 phosphorylation and nuclear translocation, and protein-protein interactions via co-immunoprecipitation.</p><p><strong>Results: </strong>Immunohistochemistry revealed significantly reduced Notch1 expression in NPC tissues, closely associated with poor differentiation and lymph node metastasis. TCGA analysis showed a 16.8% Notch1 mutation rate in HNSCC. Consensus clustering based on EMT-related genes identified subgroups with significant differences in Notch1 mutation status. Using 181 Notch1-related genes from integrated datasets, patients were further classified into three molecular subtypes (C1, C2, C3) with distinct prognosis, Notch1 mutation rates, EMT status, immune landscapes, and ferroptosis-related gene expression. Notably, the C2 subgroup exhibited the highest Notch1 mutation rate, enhanced EMT, poorest prognosis, and immune suppression.Consistent with these multidimensional datasets, scRNA‑seq analysis of primary NPC samples showed that Notch1‑high tumor cells preferentially retained a differentiated epithelial, low‑EMT/low‑stemness phenotype, further supporting an EMT‑ and metastasis‑suppressive role of Notch1 in clinical tumors. Functionally, Notch1 was downregulated across multiple NPC cell lines; knockout of Notch1 significantly promoted NPC cell proliferation, migration, invasion, and EMT both in vitro and in nude mouse models. Mechanistically, Notch1 loss increased EMT marker expression, augmented Smad3 phosphorylation and nuclear translocation, all without altering total Smad3 levels. Notch1 intracellular domain was found to interact directly with Smad3, while AKT and Smad3 interaction decreased upon Notch1 depletion, resulting in elevated Smad3 phosphorylation and EMT gene activation.</p><p><strong>Conclusi","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary Trajectory of Radioresistance in Lung Cancer Brain Metastasis Organoids.","authors":"Jianing Chen, Cize Gao, Li Wang, Leilei Wu, Boyue Pang, Chunxia Su","doi":"10.1186/s12575-026-00339-z","DOIUrl":"https://doi.org/10.1186/s12575-026-00339-z","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Cell RNA Sequencing Identifies a Distinct Epithelial Subpopulation Driving Resistance to Neoadjuvant Chemoimmunotherapy in Esophageal Squamous Cell Carcinoma.","authors":"Zuoyu Chen, Xin Yu, Kaili Liu, Yiping Zou, Xue Ding, Xiaolong Zhang, Liangliang Wu, Qiang Song, Hailong Wang","doi":"10.1186/s12575-026-00337-1","DOIUrl":"https://doi.org/10.1186/s12575-026-00337-1","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Role of Oxidative Stress-Associated Genes FKBP Prolyl Isomerase 5 in Osteoarthritis Development and Immunological Milieu.","authors":"Liangkun Huang, Zhongyu Peng, Ya Wen, Ze Zhang, Zijie Pei, Hanzhe Xu, Liangyuan Wen","doi":"10.1186/s12575-026-00336-2","DOIUrl":"https://doi.org/10.1186/s12575-026-00336-2","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of RBM15 in the Immune Escape of Non-small Cell Lung Cancer Cells Via the LncRNA EGFR-AS1/USP3/PD-L1 Axis.","authors":"Fang Li, Zhao Zhang, Shuai Liu, Yang Wang, Xiang Deng, Jiasheng Wang","doi":"10.1186/s12575-026-00329-1","DOIUrl":"https://doi.org/10.1186/s12575-026-00329-1","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNA hsa_circ_0003472 Promotes Pancreatic Ductal Adenocarcinoma Progression and Gemcitabine Resistance Via the mir-1253/ERCC1 Axis.","authors":"Cunbing Xia, Yang Chen, Xindong Yin, Yongkang Zhu, Gaoyuan Wang, Dexuan Chen, Tong Shen, Xiaojun Yang, Haijian Sun, Hong Zhu","doi":"10.1186/s12575-026-00335-3","DOIUrl":"https://doi.org/10.1186/s12575-026-00335-3","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"USP7 Drives Atherosclerosis by Promoting Ferroptosis in Vascular Endothelial Cells via the KIAA1429/NEAT1/CTCF Axis.","authors":"Bo Dong, Lu Kou, Jing-Yu Yang, Yang Li, Ning Yang","doi":"10.1186/s12575-026-00331-7","DOIUrl":"10.1186/s12575-026-00331-7","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13097630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147454823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragaloside IV Suppresses Gastric Cancer by m6A-dependent FSP1 Modulation and Ferroptosis Induction.","authors":"Wei Yin, Xi Chen, Bi Chen, Xiaodi Xu, Wenxian Guan, Haixiao Wang, Yang Su","doi":"10.1186/s12575-026-00327-3","DOIUrl":"10.1186/s12575-026-00327-3","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conventional and Novel Approaches to Establishing Mouse Models of Gastric Cancer in the Past, Present, and Potential Post-H. Pylori Infection Era.","authors":"Hui Liu, Yunxiao Ge, Kangdong Liu, Zigang Dong","doi":"10.1186/s12575-026-00333-5","DOIUrl":"10.1186/s12575-026-00333-5","url":null,"abstract":"","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13067509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Diagnosis between Sintilimab-related Autoimmune Myocarditis and Acute Myocardial Infarction.","authors":"Yihe Wu, Jiayun Nian, Hongxu Liu, Xiaolei Lai, Zihao Liu, Tengfei Li, Shenglei Qiu","doi":"10.1186/s12575-025-00267-4","DOIUrl":"10.1186/s12575-025-00267-4","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the regularities and clinical features of sintilimab-related autoimmune myocarditis, and to summarize the differential diagnosis key points between sintilimab-related autoimmune myocarditis and acute myocardial infarction.</p><p><strong>Methods: </strong>The case reports about sintilimab-related autoimmune myocarditis were searched on databases from the establishment of the database to April 1st 2024. The relevant medical records were searched on the hospital information system of Beijing Hospital of Traditional Chinese Medicine in the past 3 years. The case reports and medical records were collected for statistical analysis.</p><p><strong>Result: </strong>Twenty three cases were collected including 22 case reports and 1 case record. Most of the sintilimab-related autoimmune myocarditis were in elderly men aged 60-75 years old and occurred between the end of the first dose of treatment to the beginning of the second dose. The symptom was nonspecific such as chest tightness and palpitation, sometimes with symptom of myasthenia as muscle weakness or myositisand as muscle soreness. Elevated cardiac biomarkers and changes in electrocardiogram were common, and decreased left ventricular ejection fraction was rarely seen in echocardiography. 9 cases underwent coronary angiography or computed coronary tomography angiography, and 3 cases underwent cardiovascular magnetic resonance.</p><p><strong>Conclusion: </strong>The manifestations of sintilimab-related autoimmune myocarditis are not specific. The medication history and concomitant symptoms are of warning value. Coronary angiography or coronary computed coronary tomography angiography can be helpful when ruling out acute myocardial infarction. Cardiovascular magnetic resonance and myocardial biopsy can confirm the diagnosis. Cardiac biomarkers and the electrocardiogram can assist in diagnosis and prognosis assessment.</p>","PeriodicalId":8960,"journal":{"name":"Biological Procedures Online","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12964628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}