血管化猪视网膜离体在纳米管支架上的器官型培养。

IF 4.3 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Biological Procedures Online Pub Date : 2025-09-08 DOI:10.1186/s12575-025-00301-5

Sabrina Friebe, Solveig Weigel, Mike Francke, Stefan G Mayr

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引用次数: 0

摘要

背景：血管化视网膜移植体的器官型长期培养是药物开发、药物筛选、诊断和未来个性化医疗应用的主要挑战。在此背景下，研究人员开发并证明了一种体外培养血管化视网膜器官型的方法和方案，该方法具有最佳的组织完整性保存。方法：比较不同结构纳米管支架培养时间对血管化视网膜外植体的形态学、组织学和生化完整性及活力的影响。在此过程中，从当地屠宰场获得的猪视网膜外植体被用作血管化视网膜的范例。结论：二氧化钛纳米管阵列在生物医学信号方面具有高度可调的表面特性，因此在体外培养血管化视网膜支架方面具有很大的前景。前所未有的组织完整性维护允许在离体试验培养系统中筛选药理学药物和疾病机制，减少了对动物实验的需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Organotypic Culture of Adult Vascularized Porcine Retina Explants In Vitro on Nanotube Scaffolds.

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Organotypic Culture of Adult Vascularized Porcine Retina Explants In Vitro on Nanotube Scaffolds.

Background: Organotypic long-term cultivation of vascularized retina explants is a major challenge for application in drug development, drug screening, diagnostics and future personalized medicine. With this background, an assay and protocol for organotypic culture of vascularized retina explants in vitro with optimum tissue integrity preservation is developed and demonstrated.

Methods: Morphological, histologic and biochemical integrity as well as viability of vascularized retina explants are compared as function of cultivation time for differently structured nanotube scaffolds. In doing so, porcine retina explants obtained from a local slaughterhouse are employed as paradigm for vascularized retina.

Conclusions: We demonstrate that titania nanotube arrays are highly promising as culturing scaffold of vascularized retina explants in vitro due to highly tunable surface properties regarding biomedical signaling. The unprecedented maintenance of tissue integrity allows for screening of pharmacological drugs and disease mechanisms in an ex-vivo test-based culture system with reduced need for animal experiments.

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来源期刊

Biological Procedures Online 生物-生化研究方法

CiteScore

10.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： iological Procedures Online publishes articles that improve access to techniques and methods in the medical and biological sciences. We are also interested in short but important research discoveries, such as new animal disease models. Topics of interest include, but are not limited to: Reports of new research techniques and applications of existing techniques Technical analyses of research techniques and published reports Validity analyses of research methods and approaches to judging the validity of research reports Application of common research methods Reviews of existing techniques Novel/important product information Biological Procedures Online places emphasis on multidisciplinary approaches that integrate methodologies from medicine, biology, chemistry, imaging, engineering, bioinformatics, computer science, and systems analysis.