{"title":"Innovative bioprocess design to circumvent growth limitations and enhance astaxanthin production from slow-growing Haematococcus lacustris","authors":"Vaibhav Sunil Tambat , Reeta Rani Singhania , Tirath Raj , Cheng-Di Dong , Anil Kumar Patel","doi":"10.1016/j.biteb.2025.102492","DOIUrl":"10.1016/j.biteb.2025.102492","url":null,"abstract":"<div><div>Astaxanthin, derived from <em>Haematococcus lacustris</em>, is a highly potent bioactive compound with broad applications in pharmaceuticals, nutraceuticals, cosmetics, and animal feed. However, its commercial potential remains limited by this inefficient growth model due to high production costs and technological challenges. This study develops a two-stage mixotrophic bioprocess to enhance astaxanthin yield, aiming to surpass the typical 10–15 % astaxanthin content in dry biomass. <em>Haematococcus</em> is the best model for astaxanthin yield, yet its maximum ability could not be harnessed due to growth challenges and inadequate research. In this study, a 10 K lux light intensity showed a maximum astaxanthin content of 133.49 mg/g. Under nutrient concentration, 2×-BS,1×-HT supports biomass production up to 1.71 g/L with a unique macro- and micronutrients (2×-BS, 2×-HT) ratio, achieving a maximum astaxanthin content of 137.12 mg/g. The addition of the inducers played a significant role; SA and GA achieved maximum astaxanthin content of 189.2 and 162.60 mg/g, the maximum yield achieved so far. The metabolic flow shifted toward astaxanthin synthesis; nonetheless, 10 % lipid production was determined, which may be utilized for aquafeed or biodiesel production. This research demonstrates the <em>Haematococcus lacustris</em> model's excellent scale-up capabilities and appealing sustainability characteristics for commercial astaxanthin-lipid biorefinery. This scalable, sustainable approach aligns with SDG 9, underscoring <em>H. lacustris</em> as a viable source of astaxanthin in commercial biorefineries.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102492"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessing the effect of engineered nanoparticles on microalgae Tetradesmus obliquus, for enhanced biomass production and CO2 sequestration","authors":"Faiz Ahmad Ansari , Arvind Kumar , Humeira Hassan , Abdul Gaffar Sheik , Ismail Rawat , Sheena Kumari , Faizal Bux","doi":"10.1016/j.biteb.2025.102485","DOIUrl":"10.1016/j.biteb.2025.102485","url":null,"abstract":"<div><div>This study engineered a series of novel nickel-doped ceria nanoparticles to assess their supplementation effects on the microalgae <em>Tetradesmus</em>. Nanoparticles (NPs) were synthesized using the co-precipitation method and characterized by various techniques. The synthesized NPs were utilized to assess the effects of different dosages on the physiology (<em>F</em><sub><em>v</em></sub><em>/F</em><sub><em>m</em></sub>), chlorophyll-a content, and biochemical composition of <em>T. obliquus</em> growth. Different dosages (5, 10, 20, and 50 mg/L) of 0 %Ni@CeO<sub>2</sub>, 0.25 %Ni@CeO<sub>2</sub> and 0.5 %Ni@CeO<sub>2</sub> NPs were added in BG11 for investigation. Results show that 0.25 %Ni@CeO<sub>2</sub> (20 mg/L) NPs show the highest (2.19 g/L) biomass production compared to BG11 (1.67 g/L), and other NPs added media. Similarly, the protein content in 0.25 %Ni@CeO<sub>2</sub> (20 mg/L) medium was ~14 % and 29.74 % higher compared to BG11 and 0 %Ni@CeO<sub>2</sub> (5 mg/L) medium. The lipid and carbohydrate content in NPs-supplemented growth media shows a higher yield than BG11. The maximum higher (22.06 MJ/kg) heating value (HHV) was observed in 0.25 %Ni@CeO<sub>2</sub> (20 mg/L), followed by 20.67 MJ/kg in 0 %Ni@CeO<sub>2</sub> (20 mg/L). Similarly, the highest CO<sub>2</sub> fixation rate (0.28 gCO<sub>2</sub>/L/d) was observed in 0.25 %Ni@CeO<sub>2</sub> (20 mg/L). This research shows that 0.25 %Ni@CeO<sub>2</sub> (20 mg/L) addition in BG11 medium is a promising strategy to enhance microalgae growth, biochemical content, and CO<sub>2</sub> sequestration. Furthermore, this study provides a detailed insight into the interaction mechanism between 0.25 %Ni@CeO₂ NPs and the microalgal photosystem. The finding might be easily scaled up at the demonstration scale for enhanced biomass production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102485"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jing Gao , Jiayu Xu , Zhihong Liang , Antonio Francesco Logrieco
{"title":"Synergistic antagonism of Pleurotus pulmonarious and Trichoderma spp. against Aspergillus flavus","authors":"Jing Gao , Jiayu Xu , Zhihong Liang , Antonio Francesco Logrieco","doi":"10.1016/j.biteb.2025.102453","DOIUrl":"10.1016/j.biteb.2025.102453","url":null,"abstract":"<div><div>Biocontrol of <em>Aspergillus flavus</em> in soil is critical for preventing aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) contamination in agriculture. Mushrooms and their post-harvest spent mushroom substrate (SMS), and <em>Trichoderma</em> spp. that well-known for broad-spectrum antagonism, are underexplored for synergistic AFB<sub>1</sub> control. To explore effective biological resources for and stable control AFB<sub>1</sub> contamination, this study collected 7 mushroom species, isolated 39 <em>Trichoderma</em> strains across 9 species from their SMS, and evaluated the combined anti- <em>A. flavus</em> effects of mushrooms and <em>Trichoderma</em> spp. Results showed that <em>Pleurotus pulmonarius</em> exhibited dual functionality: promoting associated <em>T. harzianum</em> (BT-T.h-4) and <em>T. longibrachiatum</em> (BT-T.l-7) while inhibiting <em>A. flavus.</em> Its fruiting body extract increased <em>Trichoderma</em>'s inhibitory efficacy by 30 %. Under the optimal condition of spent <em>P. pulmonarius</em> substrate (fresh: sterilized =4:6), the synergistic system inhibited <em>A. flavus</em> mycelial growth by 53 %, sporulation by 96 %, and AFB<sub>1</sub> by 90 %. These findings highlight the synergistic potential <em>P. pulmonarius</em> and its associated <em>Trichoderma</em>, providing an innovative approach for reducing mycotoxin contamination as well as utilizing agricultural by-product, perfectly aligning with circular bioeconomy principles.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102453"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erik Gómez Hernández , Carlos Alberto Gómez Aldapa , Israel Sifuentes Nieves , Guadalupe Paola Radilla Serrano , Carlos Alberto Gallardo Vega , Yucundo Mendoza Tolentino , Gabriela Yolotzín Romero Zúñiga , Ernesto Hernández Hernández , Pablo González Morones
{"title":"Transforming amorphous cellulose into crystalline form using microwave irradiation in a non-reactive medium","authors":"Erik Gómez Hernández , Carlos Alberto Gómez Aldapa , Israel Sifuentes Nieves , Guadalupe Paola Radilla Serrano , Carlos Alberto Gallardo Vega , Yucundo Mendoza Tolentino , Gabriela Yolotzín Romero Zúñiga , Ernesto Hernández Hernández , Pablo González Morones","doi":"10.1016/j.biteb.2025.102504","DOIUrl":"10.1016/j.biteb.2025.102504","url":null,"abstract":"<div><div>This research investigated how microwaves transform amorphous cellulose from agro-industrial waste of barley husks (BH's) into its crystalline form. The process involves two consecutive microwave (MW) treatments of pure BH's, which show an amorphous halo at 20.3° in 2θ and have a low cellulose concentration (12.86 % by weight). After the first treatment (MW-1), the cellulose concentration increases 3.7 times, enabling the extraction of cellulose microfibrils (CM's) with a crystallinity index (CrI) of 68.27 %. The second MW treatment (MW-2) further increases cellulose concentration (by 1.7 times compared to MW-1), raising the CrI to 91.04 % and promoting the formation of type II cellulose crystals (CII). The results indicate that during MW-1, the extraction and purification of CM's dominate, consuming most of the MW energy. In contrast, during MW-2, which processes the CM's from MW-1, energy is mainly used to complete the purification of CM's and encourage the structural reorganization of amorphous regions, thereby increasing the material's crystallinity. This MW treatment provides a novel and alternative method to traditional MW processes using alkaline or acidic substances, which tend to degrade amorphous regions of cellulose and extract its crystalline fraction as nanocrystals.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102504"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biodegradation of PET and PE by tropical landfill derived Aspergillus fumigatus in modified BOD enriched medium","authors":"Priya Mohan , Fauziah Shahul Hamid","doi":"10.1016/j.biteb.2025.102534","DOIUrl":"10.1016/j.biteb.2025.102534","url":null,"abstract":"<div><div>Landfilled plastics serve as an untapped carbon reservoir, fostering selective conditions that enable microbial communities to compete and metabolize the available polymer-derived carbon. This study aims to evaluate the biodegradation potential of fungal strains isolated from Jeram Sanitary Landfill on PET, HDPE, and LDPE plastics. A total of eight fungal strains were isolated from plastic waste and tested in two liquid media, namely modified Biochemical Oxygen Demand Dilution Water (BODW) and Bushnell Haas media to determine the optimal media for degradation. Among the isolates, <em>Aspergillus fumigatus</em> (FI 5) exhibited the highest degradation efficiency, achieving 1.8 % and 2.0 % in BODW and Bushnell Haas media, respectively. A cost-benefit analysis identified BODW media as the more economical media, warranting further optimization. Degradation efficiency was significantly influenced by fungal inoculum concentration, where 1 % (w/v) inoculum yielded the highest PET weight loss (1.49 %), while a 5 % (w/v) inoculum resulted in the highest LDPE (21.93 %) and HDPE (2.79 %) weight reduction after 30 days of incubation. FTIR analysis revealed clear structural alterations in the plastic films, confirming active biodegradation. PET and LDPE degradation showed strong statistical responses (<em>p</em> = 0.0010 and <em>p</em> = 0.0100) driven mainly by incubation time. The lack-of-fit across all models (PET: 0.7599; HDPE: 0.9281; LDPE: 0.9918) further validated model reliability. Collectively, these findings highlight the potential of <em>A. fumigatus</em> to degrade PET, HDPE, and LDPE in BODW, supporting its use as an alternative approach for future plastic biodegradation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102534"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Abubakkar Siddik , Rathinavel Nithya , Alagarsamy Arun
{"title":"Integration of low cost novel membrane in 20 L stack microbial fuel cell technology: Application in powering low-power electronic devices and hydrogen generation in microbial electrolysis cell","authors":"A. Abubakkar Siddik , Rathinavel Nithya , Alagarsamy Arun","doi":"10.1016/j.biteb.2026.102543","DOIUrl":"10.1016/j.biteb.2026.102543","url":null,"abstract":"<div><div>The Novel Cement-Supported Conductive Salts (NCSCS) Proton Exchange Membrane (PEM) is been validated in lab-scale dual-chamber Microbial Fuel Cells (MFCs) using 20 L of septic tank wastewater. The NCSCS PEM is suitable for scalability (20 L × 1 L anode stacks), as proven in the present study under the different Hydraulic Retention Time (HRT) in MFCs operated in the serial and parallel connections. The highest power density (1981 ± 0.96 mW/m<sup>3</sup>) and Chemical Oxygen Demand removal (84.98 ± 1.02 %) were achieved in the serial configuration of optimized HRT 3 conditions. The current study showcases the MFC-Microbial Electrolysis Cells coupled biohydrogen production rate of 0.02 LH<sub>2</sub>/L/d, thereby demonstrating the viability of NCSCS PEM. The cost analysis revealed that the membrane accounted for only 2.28 % of total expenses, with exceptional durability lasting over 352 days without fouling or maintenance. The results demonstrate the potential for large-scale, sustainable bioelectricity generation using NCSCS membrane, making it a practical, affordable solution for wastewater-to-energy applications.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102543"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Monje Díaz , Jesús Ruiz , José Antonio Perales Vargas-Machuca , Carmen Garrido Pérez , María Dolores Macías-Sánchez
{"title":"Sustainable nutrient recovery process in brackish aquaculture effluent with microalgae technology","authors":"Julia Monje Díaz , Jesús Ruiz , José Antonio Perales Vargas-Machuca , Carmen Garrido Pérez , María Dolores Macías-Sánchez","doi":"10.1016/j.biteb.2026.102611","DOIUrl":"10.1016/j.biteb.2026.102611","url":null,"abstract":"<div><div>The rapid expansion of the aquaculture industry has led to the generation of large volumes of nutrient-rich wastewater, posing significant environmental risks if not properly managed. This study investigates <em>Parachlorella kessleri</em> for treating brackish effluent within a ‘Circular Economy’ framework. Cultivation was tested in 2 L batch and 8 L semi-continuous reactors under various nutrient supplementations. Maximum batch productivity (91.72 mgSS·L<sup>−1</sup>·d<sup>−1</sup>) required phosphorus and micronutrients addition, achieving nutrient removal below the permitted limit. In semi-continuous operation productivity reached 50.55 mgSS·L<sup>−1</sup>·d<sup>−1</sup>. Results indicate that unsupplemented batch operation maximises lipid content, while supplemented semi-continuous operation favors carbohydrate and protein enrichment. These findings demonstrate a scalable pathway for integrating microalgae into industrial aquaculture, providing reclaimed water for reuse and high-value biomass for sustainable feed or biofuels. This approach closes the nutrient loop, enhancing resource efficiency in the industry.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102611"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Adaptive laboratory evolution of Klebsiella oxytoca: Enhanced 2,3-butanediol synthesis from glycerol fermentation","authors":"Dimitris Karayannis , Nefeli Vasiliki Limnaiou , Emmanuel Rondags , Isabelle Chevalot , Seraphim Papanikolaou","doi":"10.1016/j.biteb.2025.102477","DOIUrl":"10.1016/j.biteb.2025.102477","url":null,"abstract":"<div><div>The development of highly efficient bioprocesses is of great interest in the bioeconomy era. In this study, the metabolic phenomena implicated in the biosynthesis of 2,3-butanediol (BDO), a high-value platform chemical, were thoroughly investigated and discussed during the adaptive laboratory evolution (ALE) of <em>Klebsiella oxytoca</em> ACA-DC 1581. Preliminary tests of tolerance to glycerol and BDO demonstrated that the decrease in glycerol consumption rate was initiated at a BDO concentration of <em>c</em>. 50 g/L, the latter also being associated with the conversion of BDO to acetoin. In the initial stage of cultures, the ratio of meso-BDO to L-BDO was <em>c</em>. 2.5, and then molecular equilibrium was reached when meso-BDO was converted back to acetoin. This reaction was mainly favored by residual glycerol concentrations of 12 to 15 g/L. During ALE, the gradually increasing selective pressure, namely the initial BDO concentration (BDO0), was associated with biofilm formation, when the surviving population was previously exposed to BDO threshold-level concentration. Under equivalent levels of stress, cell elongation was triggered, following cell division inhibition, this is the first study of the filamentation phenomenon in the <em>K. oxytoca</em> species to be ever reported. After 116 generations of ALE, the established threshold of <em>c</em>. 67 g/L of BDO production was surpassed by 35 %. The final BDO concentration of 90.4 g/L obtained by the BDO tolerated population is one of the highest ever documented. Finally, a successful execution of a semi-pilot scale bioreactor culture has been demonstrated. This study unveils novel underlying metabolic phenomena and demonstrates the potential of ALE as a promising strategy in the design of highly efficient BDO biosynthesis.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102477"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jyoti Moni Devi , Biplab Kumar Debnath , Rajat Subhra Das , Biplab Das
{"title":"Biochar integration in biomethanation process: Addressing limitations and unlocking performance gains","authors":"Jyoti Moni Devi , Biplab Kumar Debnath , Rajat Subhra Das , Biplab Das","doi":"10.1016/j.biteb.2025.102512","DOIUrl":"10.1016/j.biteb.2025.102512","url":null,"abstract":"<div><div>Anaerobic digestion (AD) is a well-established biological process used to convert and treat complex substrates. However, its widespread implementation is constrained by several challenges. The use of additives such as biochar (BC) is proposed to address these challenges. This review summarizes the applications of BC in AD, including parent materials for BC-assisted AD systems, substrates used in AD, BC's function, including parent materials for BC-assisted AD systems, BC's role in AD, engineered BC-assisted AD approaches, and its application in biogas purification. Additionally, to address the expansion of BC incorporation, a strengths-weaknesses-opportunities-threats (SWOT) analysis is conducted. Despite the numerous benefits associated with BC, its cost-effectiveness compared to energy gains is vital. A key issue identified is the lack of large-scale studies. Limited studies that have examined the long-term regulatory effects of BC in full-scale reactors. Future research can concentrate on optimizing BC performance and refining production methods to enhance cost-effectiveness. Effective policies can incentivize the use of biogas and BC expertise, endorsing their broader application.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102512"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sinenhlanhla L. Mweli, Isaac A. Sanusi, Lorika S. Beukes, Gueguim E.B. Kana
{"title":"Enhancing bioethanol production: Synergistic effects of nano-Fe3O4, inoculum, large language model-assisted additive optimization and economic implications","authors":"Sinenhlanhla L. Mweli, Isaac A. Sanusi, Lorika S. Beukes, Gueguim E.B. Kana","doi":"10.1016/j.biteb.2026.102600","DOIUrl":"10.1016/j.biteb.2026.102600","url":null,"abstract":"<div><div>This study presents a novel approach that enhanced bioethanol production through nano-based <em>S. cerevisiae</em> inoculum development, antioxidant (ascorbic acid) and surfactant (Tween-80) incorporation. Response Surface Methodology (RSM) was initially used to model Fe<sub>3</sub>O<sub>4</sub> NPs (0–0.1 wt%), temperature (28–30 °C), and exposure time (12–18 h) towards high <em>S. cerevisiae</em> specific growth rate and biomass concentration for improved bioethanol production. Experimental validation yielded 0.234 ± 0.019 h<sup>−1</sup> and 3.395 ± 0.102 g/L respectively, corresponding to a 13% and 8% enhancement over the control. The fermentation performance of the optimized inoculum on pretreated potato residues was enhanced (>55%). Moreover, the use of context-driven retrieval-augmented generation (CD-RAG) large language model (LLM) assisted the optimization of Tween-80 and ascorbic acid inclusion for improved specific growth rate (1.16-fold) and biomass concentration (1.11-fold). Economic assessment of the inoculum development strategy points to a potential profitable approach for producing bioethanol with shorter payback time (<10 years) at higher processing capacity. These findings have provided valuable insights to improve bioethanol yield using Fe<sub>3</sub>O<sub>4</sub> NPs and CD-RAG LLM in inoculum development.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102600"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}