Bolu Sun , Xinlan Wang , Ying Lv , Xuanxiu Da , Miao Zhou , Jiali Kang , Haiying He , Chenyu Qin , Xiangdong Wang , Lin Yang , Quhuan Ma , Hongxia Shi , Xiaofeng Shi
{"title":"Screening of biocontrol agents against lily bulb rot caused by F. oxysporum and research on their fermentation process","authors":"Bolu Sun , Xinlan Wang , Ying Lv , Xuanxiu Da , Miao Zhou , Jiali Kang , Haiying He , Chenyu Qin , Xiangdong Wang , Lin Yang , Quhuan Ma , Hongxia Shi , Xiaofeng Shi","doi":"10.1016/j.biteb.2025.102267","DOIUrl":"10.1016/j.biteb.2025.102267","url":null,"abstract":"<div><div>The bulb rot disease caused by <em>Fusarium oxysporum</em> (<em>F. oxysporum</em>) in Lanzhou lily (<em>Lilium davidii</em> var. <em>unicolor</em>) is one of the most difficult diseases to detect in the early stages of cultivation, seriously threatening the yield and quality of Lanzhou lily. Currently, the main prevention method is pesticide drenching and broadcasting, apt to excessive residues, reduced quality, and environmental contamination. Thus, there is an urgent need for green and sustainable control strategies. However, screening endophytes for the development of biological control agents has been more popular in recent years owing to their strong antifungal properties and ability to settle in plant tissues asymptomatically. Initially, 21 endophytic fungal strains were isolated from healthy lilies collected in regions with high incidence of bulb rot disease in Lanzhou using the tissue separation method. Using the growth rate and plate confrontation methods, five strains (ST5, ST8, ST12, ST14, and ST20) were selected for their significant inhibitory effect on <em>F. oxysporum</em>, with inhibition rates exceeding 30 %. Notably, strain ST12 showed an inhibition rate of 74 %, identified as <em>Penicillium crustosum</em>. Simultaneously, response surface methodology optimized the ST12 fermentation process, revealing that at 27 °C, 11 d of fermentation, and at a number of the inoculated fungal cakes of 3.5, the inhibition rate against <em>F. oxysporum</em> reached 74.50 %. In addition to choosing a biocontrol agent for the efficient management of the <em>F. oxysporum</em>-caused bulb rot disease in Lanzhou lily, this study offered scientific guidance and technical assistance for the creation of biocontrol agents from plant endophytic fungi.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102267"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biodegradation of polystyrene microplastics by Pseudomonas sp. WD23: Toxicity assessment using freshwater snail Pomacea canaliculata","authors":"Louella Concepta Goveas , Sathwik Shettigar , Srasha Rai , Sangeeth Vinod Kumar , Shaun Linal Dsouza , Gokulakrishnan Murugesan , Raja Selvaraj , Ramesh Vinayagam","doi":"10.1016/j.biteb.2025.102261","DOIUrl":"10.1016/j.biteb.2025.102261","url":null,"abstract":"<div><div>This study presents degradation of polystyrene microplastics (PS MPs) by petroleum hydrocarbon degrading <em>Pseudomonas</em> sp. WD23 and toxicity assessment on <em>Pomacea canaliculata.</em> Exposure of 100 mg/L PS MPs to <em>Pseudomonas</em> sp. WD23 for 42 days, led to 28.40 ± 0.57 % weight reduction, following first order kinetics with rate constant and half-life of 0.0073 day<sup>−1</sup> and 94.95 days. Scanning electron microscopy and Fourier transform infrared spectrometry analysis revealed bacterial biofilm presence, smoothening, hole formation with confirmed oxidative degradation. Comparative toxicity of 100 and 200 mg/kg sediment of pristine and degraded PS MPs on <em>Pomacea canaliculata</em> was analyzed by behavior, mortality, enzymatic and non-enzymatic stress responses. Contact with pristine PS MPs caused increased reaction time with marked catalase (0.0095–0.0113 mmol/(min·mg protein)) and radical scavenging activity (73.68–78.96 %). Exposure to degraded PS MPs yielded slight catalase (0.0029–0.0032 mmol/(min·mg protein)) and moderate radical scavenging activity (63.15–65.78 %).</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102261"},"PeriodicalIF":0.0,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Catalytic steam-gasification of olive pomace biowaste for enhanced hydrogen production","authors":"Aytenew Getaye , Touria Moudakkar , Sébastien Vaudreuil","doi":"10.1016/j.biteb.2025.102249","DOIUrl":"10.1016/j.biteb.2025.102249","url":null,"abstract":"<div><div>Olive pomace (OP), a major biowaste in Morocco, is discarded in a non-environmentally-friendly manner. Harnessing this potential as a bioenergy source fosters a circular economy. Among the various options for biomass utilization, gasification is the most viable pathway because of its environmental advantages. However, low hydrogen yields and high tar and CO<sub>2</sub> emissions are significant challenges in biomass gasification. This study used Aspen plus® commercial software to develop olive pomace catalytic steam gasification. The system was evaluated by varying the reaction temperature, steam-to-biomass ratio, equivalent ratio, and CaO-to-biomass ratio. The simulation results demonstrated that the synergetic effect of steam and the CaO catalyst likely catalyzes volatile cracking and CO<sub>2</sub> absorption, driving steam reforming and water gas shift reactions forward, thus increasing the H<sub>2</sub> yield. The economic results support the viability of OP gasification, with a 2.43-year payback period and a net profit of $257.88 per ton of OP.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102249"},"PeriodicalIF":0.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Process development of zeolite-Y-catalyst from Kulende MetaKaolin: Experimental data modelling, process optimization, computer-aided scale-up techno-economics and zeolite-Y-catalyst application for biodiesel production","authors":"Kazeem Kolapo Salam , Emmanuel Olusola Oke , Dauda Olurotimi Araromi , Mujidat Omolara Aremu , Christopher Tunji Oloyede , Idayat Adebukola Olowonyo , Monsuru Olatunji Dauda , Temitope Olabisi Adesina","doi":"10.1016/j.biteb.2025.102262","DOIUrl":"10.1016/j.biteb.2025.102262","url":null,"abstract":"<div><div>Most existing studies on zeolite-Y synthesis remain at the laboratory-scale, limiting process design and industrial application. This study optimized key variables: acid concentration, quench time, and NaOH:KMK for the synthesis of zeolite-Y from the Kulende Metakaolin. The optimal zeolite-Y synthesis condition was simulated, scaled-up and its feasibility study was performed through Techno economic analysis. The synthesized zeolite-Y was tested in the transesterification process. Optimal conditions (65.97 % acid, 3.89 min quenching, and 1.99 NaOH:KMK) was used for zeolite-Y synthesis. The lab-optimized process was simulated and scaled up to 4000 kg/batch, achieving 104 batches/year, 0.65 kg/min production rate, and 5.8 million kJ total energy consumed. Techno-economic analysis confirmed feasibility, with a 15-year investment yielding a NPV of $9.59 million, 27.23 % IRR, and 27 % ROI. The synthesized zeolite-Y was successfully tested for biodiesel production, with both products respectively characterized. This study demonstrates that zeolite-Y from KMK is scalable, economically viable, and suitable for industrial biodiesel production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102262"},"PeriodicalIF":0.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biochar from anaerobic digestates: Production, characteristics and applications in wastewater remediation","authors":"Niyati P. Patel , M. Mansoor Ahammed","doi":"10.1016/j.biteb.2025.102258","DOIUrl":"10.1016/j.biteb.2025.102258","url":null,"abstract":"<div><div>Anaerobic digestate, a byproduct of biogas production, poses significant challenges related to its disposal and environmental impacts. Converting this waste into biochar offers a sustainable solution by transforming the digestate into a value-added product with diverse applications. This review reports the production of biochar from different anaerobic digestates, focusing on its modifications, characteristics and potential applications. The characteristics of the digestate which can influence the biochar characteristics are first discussed. Various biochar production methods and the processes used to modify the biochar characteristics are compared. The reported applications of digestate-derived biochar specifically for the removal of different classes of contaminants including heavy metals, dyes, nutrients and organic micropollutants, in addition to its use in the anaerobic treatment process, are then discussed. This review shows that while the use of digestate-derived biochar appears promising, several critical gaps remain. Further studies on its long-term applications using real water/wastewater, standardising and optimising the biochar production process, and cost-benefit analysis are needed to scale up its use in practical applications.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102258"},"PeriodicalIF":0.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimizing extraction process of Fish Protein Concentrate from Katley fish (Copper mahseer) using response surface methodology","authors":"Hijam Merina Devi , Basant Kumar Singh , Pinku Chandra Nath , Vinay Kumar Pandey , Sarvesh Rustagi , Sajesh Chettri","doi":"10.1016/j.biteb.2025.102259","DOIUrl":"10.1016/j.biteb.2025.102259","url":null,"abstract":"<div><div>Fish Protein Concentrate (FPC) has evolved as a possible alternative to fulfil the growing worldwide demand for low-cost, animal-based protein. The Mahseer fish, specifically the Katley fish (Copper Mahseer, <em>Neolissochilus hexagonolepis</em>), has tremendous potential as a raw material for FPC preparation. The Katley fish, recognized as the state fish of Sikkim, found in the Teesta and Rangeet rivers, making it an important resource for this purpose. The present study aimed to optimize the preparation of FPC from Katley fish by focusing on moisture and fat removal, while improving its nutritional profile. The effect of three independent variables (temperature (°C), time duration (minutes), and solvent-to-fish flesh ratio (v/w)) on crude protein content, moisture content, fat content, and product yield were studied by employing Response surface methodology, particularly Central Composite Design (CCD). The optimum parameters for production of FPC production were found to be as 60 °C temperature, 30 min of processing time duration, and 2:1 (v/w) of solvent-to-fish flesh ratio. At optimum condition, the resulting FPC was found to have a 17.80 % product yield, 76.98 % crude protein content, 4.96 %, moisture content and 0.76 % crude fat content. Since the resulting FPC contains >67 % crude protein, and has no >0.80 % fat, it is categorised as type A FPC.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102259"},"PeriodicalIF":0.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bingbing Xing , Hao Zhu , Han Xiao , Chengyu Ma , Weijie Zhou , Wei Gao , Qilin Zhu , Huanyu Bao , Yunze Ruan
{"title":"Microbial inoculation simultaneously promotes humification and the removal of antibiotic resistance genes during kitchen waste composting","authors":"Bingbing Xing , Hao Zhu , Han Xiao , Chengyu Ma , Weijie Zhou , Wei Gao , Qilin Zhu , Huanyu Bao , Yunze Ruan","doi":"10.1016/j.biteb.2025.102257","DOIUrl":"10.1016/j.biteb.2025.102257","url":null,"abstract":"<div><div>This research investigated the impact of microbial inoculation on humification and the dissemination of antibiotic resistance genes (ARGs) during kitchen waste composting. The findings showed that microbial inoculation (J) obviously facilitated the humification during composting, with the humic acid (HA) content increased by 12.11 % compared to the control (CK) without inoculation. The dissolved organic matter (DOM) of 3D-EEM spectrum showed that microbial inoculation promoted the humic-like substances formation and the degradation of soluble microbial byproducts. Random forest and correlation analysis indicated that increase in HA synthesis in J treatment during composting was attributed to the decomposition of fulvic acid (FA) and organic matter (OM). Moreover, microbial inoculation reduced the ARGs abundance, which decreased by 32.56 %, compared to CK. The random forest analysis and structural equation modeling illuminated that microbial inoculation prolonged thermophilic phase and reduced the abundance of mobile genetic elements (MGEs), therefore decreasing the prevalence of ARGs during composting. This research elucidated the potential mechanisms of microbial inoculation on HA formation, and revealed that the prolonged thermophilic phase promoted the removal of ARGs. It offers valuable insights for the simultaneous promotion of humification and the removal of ARGs during composting.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102257"},"PeriodicalIF":0.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arianna Carrara , Andrea Goglio , Amin Narimani , Gabriele Soggia , Elisa Clagnan , Patrizia De Nisi , Fabrizio Adani
{"title":"Selection of waste-derived inocula for CO2 bio-electro-recycling: Integration of microbial electrosynthesis and anaerobic digestion on charcoal functionalized electrodes","authors":"Arianna Carrara , Andrea Goglio , Amin Narimani , Gabriele Soggia , Elisa Clagnan , Patrizia De Nisi , Fabrizio Adani","doi":"10.1016/j.biteb.2025.102260","DOIUrl":"10.1016/j.biteb.2025.102260","url":null,"abstract":"<div><div>Microbial electrosynthesis (MES) has been widely used to produce volatile fatty acids (VFAs) from CO<sub>2</sub>. In this technology, the applied potential, the source of the inoculum, and the cathode material are key parameters influencing process performance. This study evaluated different waste-derived inocula to identify the most effective one, which was then used as inoculum in MES bioreactors equipped with activated charcoal-functionalized cathodes at different cathodic potentials: −0.8 V and − 1.0 V vs Ag/AgCl to promote acetate production. Physicochemical parameters were continuously monitored and microbial community composition was characterized. MES experiments were conducted in double-chamber bioreactors using 100 % <em>v</em>/v CO<sub>2</sub> feed. The system operating at −1.0 V outperformed the one at −0.8 V MES, converting 60.4 ± 5.1 % of CO<sub>2</sub> into acetic acid, with a daily production rate of 98.6 ± 7.5 mg L<sup>−1</sup> d<sup>−1</sup>. Productivity was used to estimate the CO<sub>2</sub> emission from biogas from Italian anaerobic digestion plants that can be avoided by converting this CO<sub>2</sub> into acetate.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102260"},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elif Toy , Beyza Tutku Bıçakcı , Cansu Erdem , Bahar Sincar , Feyza Özdemir , Melike Keskin , Dilek Yalçın , Gamze Turan , Alberto Baldelli , Oguz Bayraktar
{"title":"Optimization of extraction for antioxidant and photoprotective bioactive compounds from Ulva rigida","authors":"Elif Toy , Beyza Tutku Bıçakcı , Cansu Erdem , Bahar Sincar , Feyza Özdemir , Melike Keskin , Dilek Yalçın , Gamze Turan , Alberto Baldelli , Oguz Bayraktar","doi":"10.1016/j.biteb.2025.102253","DOIUrl":"10.1016/j.biteb.2025.102253","url":null,"abstract":"<div><div>This study presents the development and optimization of a green extraction bioprocess for <em>Ulva rigida</em>, aiming to efficiently recover bioactive compounds with antioxidant and photoprotective properties using response surface methodology (RSM). A Box-Behnken design evaluated the combined effects of ethanol concentration (0–100 %), solid-to-liquid ratio (20–40 mL g<sup>−1</sup>), and extraction time (8–24 h) on extraction yield, antioxidant capacity, and sun protection factor (SPF). All variables significantly impacted extract quality, with ethanol concentration being the most influential. Optimal conditions (100 % ethanol, 1:20 ratio, 16 h) yielded an extract with SPF 11.61 and antioxidant activity of 16.91 mg Trolox/g. Preparative thin-layer chromatography (TLC) of the optimized extract produced three bioactive fractions exhibiting up to 2.3-fold higher SPF and 5-fold greater antioxidant activity. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed selective enrichment of carotenoids and polyphenols. These findings demonstrate the value of statistically optimized, bioactivity-guided extraction in enhancing <em>Ulva</em>-derived ingredients for cosmeceutical use.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102253"},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Design and characterization of BPAC-SA beads synergistic phytoremediation with Eichhornia crassipes for enhanced adsorption of Ni2+ ions from industrial effluents: IoT real-time monitoring","authors":"Salsyah Billa Rasdin , Dahlang Tahir , Heryanto Heryanto , Vicram Setiawan , Asnan Rinovian , Ahmed Akouibaa , K. Veeravelan","doi":"10.1016/j.biteb.2025.102255","DOIUrl":"10.1016/j.biteb.2025.102255","url":null,"abstract":"<div><div>Here, we demonstrate the effective use of sodium alginate (SA) and banana peel-activated carbon (BPAC) combined <em>Eichhornia crassipes</em> for phytoremediation as sustainable adsorbents for Ni<sup>2+</sup> ion removal from nickel industry effluents. SA beads' adsorption capacity and removal efficiency exhibited 1357 mg/g and 90.5 %, while BPAC-SA composite beads achieved 1393 mg/g and 92.9 %. Notable bead expansion post-adsorption confirmed strong pollutant interaction, particularly in BPAC-SA beads. Structural characterization via XRD revealed the slight transition from amorphous to crystalline phases, confirming the successful incorporation of BPAC into the SA matrix through ionotropic gelation methods. The BPAC-SA beads displayed a rough, porous morphology with enhanced surface area and bonding potential. At the same time, FTIR spectra showed functional group shifts—especially in <img>OH, <img>CH, carbonyl, and carboxylate bands indicating strong interactions and new bond formations. Real-time monitoring using Internet of Things devices successfully implemented and validated the adsorption kinetics and process efficiency.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102255"},"PeriodicalIF":0.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}