Sustainable biocatalyst for textile dye effluent treatment using immobilized turnip peroxidase on polydopamine coated sawdust

Q1 Environmental Science

Bioresource Technology Reports Pub Date : 2025-04-15 DOI:10.1016/j.biteb.2025.102118

Rasha A. Youssef , Abdul Aziz M. Gad , Rehab Elmougy , Abdel-Aziz Fatouh Abdel-Aziz , Mohamed A. Yassin

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Abstract

Immobilizing enzymes on sustainable supports enhances wastewater treatment efficiency and promotes environmental sustainability. This study investigates polydopamine-coated sawdust as a sustainable support for immobilizing turnip peroxidase (TP) to mitigate textile dye effluent pollution. Sawdust was dip-coated with a bio-adhesive polydopamine layer, facilitating covalent TP immobilization. The TP was isolated and purified to homogeneity from the roots of Brassica rapa. Compared to free TP, the immobilized enzyme exhibited improved thermal and pH stability and was reusable for 25 consecutive catalytic cycles. Furthermore, it demonstrated synergistic potential for decolorization of fast green FCF (72 %) and crystal violet (88 %) dyes within 120 min through adsorption and catalytic enzymatic oxidation. Eventually, immobilization of TP on sustainably sourced and cost-effective polydopamine-coated sawdust has the potential to serve as an efficient biocatalyst for eco-friendly enzymatic remediation of dye-contaminated wastewater.

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聚多巴胺包覆木屑固定化萝卜过氧化物酶处理纺织染料废水的可持续生物催化剂研究

在可持续载体上固定化酶可以提高废水处理效率，促进环境的可持续性。本文研究了聚多巴胺包被木屑作为固定萝卜过氧化物酶（TP）的可持续载体，以减轻纺织染料废水的污染。在木屑上浸渍一层生物黏附的聚多巴胺层，促进共价TP的固定。从油菜根茎中分离纯化了TP。与游离TP相比，固定化酶表现出更好的热稳定性和pH稳定性，并可重复使用25个连续催化循环。此外，通过吸附和催化酶氧化，在120 min内显示出对快速绿色FCF（72%）和结晶紫（88%）染料的协同脱色潜力。最终，将TP固定在可持续来源且具有成本效益的聚多巴胺涂层锯末上，有可能作为一种高效的生物催化剂，用于染料污染废水的生态友好型酶修复。

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