{"title":"Enhancement of physical and electrochemical activity of polyvinyl alcohol/chitosan/bentonite based membrane through sulfonation for the bioelectricity generation in microbial fuel cell","authors":"Sandhya, Nethavath Prashanth, Banoth Vamshikrishna, Astha, Ganesh Swain","doi":"10.1016/j.biteb.2026.102805","DOIUrl":"10.1016/j.biteb.2026.102805","url":null,"abstract":"<div><div>The feasibility and large-scale applicability of the microbial fuel cell (MFC) is limited due to the high fabrication cost and oxygen permeability of the commercial membrane. The present study demonstrated the preparation of a polymeric membrane via a cost-effective approach to enhance the physical and electrochemical performance of the raw membrane. In this direction, bentonite (BT), an abundant and low-cost natural clay, is employed as a filler to the polymeric membrane composed of polyvinyl alcohol (PVA) and chitosan (CS). The sulfonation of BT significantly increased the ion exchange capacity and proton mass transfer coefficient of the membrane and obtained to be 1.2 mmol/g of dry membrane and 6.59 × 10<sup>−6</sup> cm/s, respectively. Notably, the oxygen diffusivity was reduced by 57% with the sulfonation of BT. Moreover, the sulfonated membrane offered more than 5.28 and 1.51 times of power density and current density, respectively, compared to the unmodified membrane. Furthermore, the present MFC could be able to remove 85.20% of the initial chemical oxygen demand with a coulombic efficiency of 25.79%. Despite the lower proton conductivity of the prepared polymeric membrane compared to the commercial one (i.e., Nafion-117), its lower cost highlights the significant potential for practical applications in MFC technology in bioelectricity generation and wastewater treatment.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102805"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dasinaa Subramaniam, Thu Huong Pham, Charles F. Manful, Lakshman Galagedara, Manokararajah Krishnapillai
{"title":"Valorization of pulp and paper mill sludge through vermiculture: A nutritional assessment of Eisenia fetida as sustainable feed alternative","authors":"Dasinaa Subramaniam, Thu Huong Pham, Charles F. Manful, Lakshman Galagedara, Manokararajah Krishnapillai","doi":"10.1016/j.biteb.2026.102735","DOIUrl":"10.1016/j.biteb.2026.102735","url":null,"abstract":"<div><div>Pulp and paper mill sludge (PPMS), a significant byproduct of the paper industry, poses considerable environmental and disposal challenges. However, its organic content offers potential for reuse as a nutrient-rich substrate in vermiculture. This study aimed to evaluate the suitability of PPMS for the cultivation of <em>Eisenia fetida</em>, for enhancing the nutritional quality of earthworm biomass for potential application in animal feed and as a sustainable bioresource. <em>E. fetida</em> were cultured in PPMS amended with various substrates; vegetable peels (TSV), soil (TSS), fresh cow manure (TSCF), and composted cow manure (TSCC) in a 2:1 ratio while PPMS alone (TS) served as the control. After eight weeks of vermiculture, harvested <em>E. fetida</em> were analyzed for proximate composition; moisture and dry matter content, total soluble protein, lipids, total phenolics and antioxidants, total soluble sugar, vitamins, and minerals contents. Moisture (86.7–89.6%) and dry matter content (10.4–13.3%) differed significantly (<em>p</em> < 0.05). In addition, significant differences (<em>p</em> < 0.05) were found in soluble protein (17.8–21.7%), lipid (7.8–13.3%), total phenolics (14.3–23.5 mg g<sup>−1</sup>), antioxidant capacity (5.2–27.6 μmol TE g<sup>−1</sup>), and total soluble sugar (0.8–1.3%) contents. A strong positive correlation (R<sup>2</sup> = 0.92) was observed between total phenolics and antioxidant activity, with the highest values in TS, followed by TSV, and TSS. Although the TS yielded significantly (<em>p</em> < 0.05) lower nutritional indices for total soluble protein and sugar, PPMS (TS) exhibited significantly reduced (<em>p</em> < 0.05) concentrations of potentially toxic elements (Co, Pb, Cd) and enhanced vitamins compared to amended treatments. These findings suggest that <em>E. fetida</em> cultured in PPMS, especially without amendments, can accumulate essential nutrients while exhibiting a lower risk of toxic element bioaccumulation. <em>E. fetida</em> tested from PPMS amended with cow manure, vegetable peels and soil may serve as an alternative protein source for poultry and aquaculture feed, as well as a viable approach for industrial PPMS valorization.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102735"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148499437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Javier Mauricio Loaiza, Alberto Palma, Juan Carlos Garcia, Manuel Jesús Diaz, Ascensión Alfaro
{"title":"Sequential cascade biorefinery for the valorization of spent coffee grounds: Energy, biodiesel, furfural, and biochar production","authors":"Javier Mauricio Loaiza, Alberto Palma, Juan Carlos Garcia, Manuel Jesús Diaz, Ascensión Alfaro","doi":"10.1016/j.biteb.2026.102754","DOIUrl":"10.1016/j.biteb.2026.102754","url":null,"abstract":"<div><div>This study presents a sequential biorefinery approach for the integrated valorization of spent coffee grounds (SCG) within a circular economy framework. The process comprises multiple operational units. Initially, SCG are pelletized for direct combustion, demonstrating promising energy potential. In the second unit, coffee oil is extracted via Soxhlet using hexane, isopropanol, and ethanol, and subsequently converted into biodiesel through transesterification, yielding a biofuel with a favorable fatty acid profile. The third unit involves ultrasound-assisted cold alkaline extraction (CAE) of the residual solid to selectively recover hemicelluloses with minimal degradation. Optimal CAE conditions (40 °C, 90 min, 100 g L<sup>−1</sup> NaOH) enhanced hemicellulose yield while maintaining high calorific value (21.529 kJ kg<sup>−1</sup>), comparable to the raw material (21.560 kJ kg<sup>−1</sup>). The hemicellulose-rich liquor was subjected to acid hydrolysis for furfural production, with maximum yield (590.3 mg L<sup>−1</sup>) achieved under central experimental conditions (2% acid, 160 °C). The solid residue from hydrolysis underwent pyrolysis to produce biochar. Kinetic analysis of the pyrolysis process using the Flynn–Wall–Ozawa method on TGA data enabled determination of activation energy without assuming a specific reaction model. The results confirm the feasibility of integrating thermochemical and biochemical pathways for SCG valorization, supporting the development of sustainable biorefineries and contributing to renewable energy and chemical production within circular economy strategies.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102754"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Irin Mary Mathew, Esther J. Rachel, Nageswar Sahu, Raushan Quraishi, Biswanath Mahanty
{"title":"Machine learning–assisted optimization of process conditions for microbial valorization of banana peel extract into pullulan","authors":"Irin Mary Mathew, Esther J. Rachel, Nageswar Sahu, Raushan Quraishi, Biswanath Mahanty","doi":"10.1016/j.biteb.2026.102761","DOIUrl":"10.1016/j.biteb.2026.102761","url":null,"abstract":"<div><div>Agro-waste valorization offers a sustainable route to reduce the cost of biopolymer production. This study utilized banana peel extract as a low-cost substrate for pullulan production by <em>Aureobasidium pullulans</em>, adopting an integrated experimental and machine learning (ML) framework. Central composite design (CCD) experiments were adopted to map the effect of varying C/N ratio, inoculum concentration, and incubation time on biomass growth and pullulan production. Hyperparameter-optimized artificial neural network (ANN) and random forest (RF) models were developed using a Gaussian-noise augmented experimental dataset. The ANN models demonstrated superior predictive accuracy (R<sup>2</sup> > 0.998) and lower RMSE values for biomass (0.0151 g L<sup>−1</sup>) and pullulan (0.0545 g L<sup>−1</sup>) than the RF models. Shapley Additive Explanation (SHAP) identified inoculum concentration as the most influential factor governing both responses. Multi-objective optimization generated Pareto-optimal solutions, revealing a trade-off between biomass (0.23–1.35 g L<sup>−1</sup>) and pullulan production (1.5–9.4 g L<sup>−1</sup>). Overall, the proposed ML-integrated optimization framework supports efficient agro-waste valorization and sustainable pullulan production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102761"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioconversion of the forest wastes into oyster mushrooms as a circular bio-economy with promise for health aspects of blue tilapia","authors":"Arul Kumar Murugesan, Pradheena Vairaganthan, Raju Subha","doi":"10.1016/j.biteb.2026.102817","DOIUrl":"10.1016/j.biteb.2026.102817","url":null,"abstract":"<div><div>Forestry residues and spent mushroom substrate (SMS) represent two major lignocellulosic waste streams require for sustainable management. The current study explores a circular bioresource approach by utilizing these wastes for mushroom cultivation and subsequently applying the derived products in aquaculture. <em>Pleurotus flabellatus</em> was used for the bioconversion of forestry residues and SMS. The nutritional efficacy of the resulting mushroom extract was evaluated as a partial replacement for fish meal in the experimental diets of <em>Oreochromis aureus</em> (blue tilapia). A ten-week feeding trial was conducted using seven experimental diets containing graded levels of mushroom extract: M0 (0 g kg<sup>−1</sup>), M1 (0.005 g kg<sup>−1</sup>), M2 (0.01 g kg<sup>−1</sup>), M3 (0.02 g kg<sup>−1</sup>), M4 (0.03 g kg<sup>−1</sup>), M5 (0.04 g kg<sup>−1</sup>), and M6 (0.05 g kg<sup>−1</sup>). Growth performance, metabolic biomarkers, antioxidant activity, and histomorphological characteristics of intestinal and hepatic tissues were assessed. Fish fed mushroom-supplemented diets exhibited improved antioxidant responses, along with increased plasma protein and glucose levels compared to the control. Moderate inclusion levels (particularly M3–M5) showed better overall performance, although growth parameters did not differ significantly among the treated groups. These findings demonstrate that forestry residues and SMS can be effectively valorized into a functional aquafeed ingredient, contributing to the circular economy, waste reduction and sustainable aquaculture practices.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102817"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mesh filtration pretreatment enhances methane production, microbial stability, and process robustness in palm oil mill effluent anaerobic digestion","authors":"Cheerapat Supawatkon, Sasithorn Rungjaroenchaiwat, Thamonwan Woraruthai, Suttavadee Junyakul, Thanyaporn Wongnate","doi":"10.1016/j.biteb.2026.102806","DOIUrl":"10.1016/j.biteb.2026.102806","url":null,"abstract":"<div><div>Palm oil mill effluent (POME) is a high-strength agro-industrial wastewater with strong potential for bioenergy recovery, yet its anaerobic digestion (AD) performance is often limited by suspended solids, dilution, and compositional variability. This study investigated mesh sieving as a simple and low-cost solid-liquid separation pretreatment to improve methane production from POME. Batch AD experiments were conducted at 37 °C under static conditions using four treatment configurations combining filtered and unfiltered inoculum and POME. Process performance was evaluated through methane production kinetics, volatile fatty acid (VFA) dynamics, microbial community profiling, and economic feasibility assessment. Mesh sieving markedly enhanced methane recovery, increasing yields by 32.9–40.5% compared with the untreated control. The highest methane yield, 249.7 mL CH<sub>4</sub>/g VS, was obtained when both inoculum and POME were filtered through a 0.60 mm mesh. VFA analysis showed efficient acetate consumption to below inhibitory levels, indicating stable methanogenic conversion. Microbial analysis revealed enrichment of hydrolytic and syntrophic bacteria, particularly <em>Syntrophomonas</em> and <em>Proteiniphilum</em>, together with dominance of the acetoclastic methanogen <em>Methanosaeta</em> and increased abundance of hydrogenotrophic methanogens including <em>Methanoculleus</em> and <em>Methanospirillum</em>. Economic feasibility analysis suggested that applying mesh sieving to raw POME could increase potential annual electricity generation from 1.56 × 10<sup>7</sup> to 2.09 × 10<sup>7</sup> kWh, with projected revenue rising from USD 938,280 to USD 1,253,900. These findings demonstrate that mesh sieving is an effective, scalable pretreatment strategy for improving methane recovery and energy valorization from POME.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102806"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phosphorus-rich liquid extraction strategies to enhance phosphorus removal in aerobic granular sludge treating municipal wastewater","authors":"Elsayed Mady, Jan Oleszkiewicz, Qiuyan Yuan","doi":"10.1016/j.biteb.2026.102852","DOIUrl":"10.1016/j.biteb.2026.102852","url":null,"abstract":"<div><div>This study evaluates two aerobic phosphorus (P) starvation-based operational strategies, intermittent and continuous extraction of P-rich liquid at the end of the anaerobic phase, using aerobic granular sludge, to enhance P removal performance in municipal wastewater treatment while preserving granule stability. Three reactors were operated for 127 days: R1 with intermittent P-rich liquid extraction, R2 with continuous extraction, and a control (Rc). Results showed R1 had the highest P-uptake rate (1.42 mgP/gMLVSS·h) and P removal efficiency (≥ 90%), outperforming other reactors. While the extraction strategies affected granule stability, R1 successfully re-granulated, whereas R2 granules continued to disintegrate. The P-rich liquids extracted from R1 demonstrated high potential for P recovery via struvite precipitation. Sludge samples from R1 at the end of operation had the highest bacterial richness and diversity. Overall, intermittent extraction achieved consistently low effluent P and opened pathways for resource recovery, underscoring its value for future circular wastewater treatment strategies.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102852"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytoremediation of paper mill effluent contaminated with lead using EDTA and citric acid supported aquatic plant species: Eichhornia crassipes, Lemna minor, and Salvinia molesta","authors":"Jyotsna Kaushal, Punit Bhardwaj, Lata Rani, O.P. Yadav, Chander Prabha, Anupam Kumar Bairagi, Md Khairul Amin","doi":"10.1016/j.biteb.2026.102733","DOIUrl":"10.1016/j.biteb.2026.102733","url":null,"abstract":"<div><div>In the current study aquatic plant species- <em>Pontederia crassipes, Lemna minor, and Salvinia molesta</em> - have been employed along with ethylenediamine tetra-acetic acid (EDTA) and citric acid as chelating agents for the removal of lead (Pb) from effluent wastewater collected from a pulp and paper mill at Yamuna Nagar (30.133°N 77.288°E) in India. The removal of Pb from the wastewater was more in both the case of EDTA and citric acid-assisted phytoremediation treatments compared to when no mediator ligand was used. It was revealed that the chelate-mediated <em>Pontederia crassipes</em> exhibited the highest uptake of lead among the studied plant species. Compared to citric acid, using EDTA as a mediator for the studied plant species proved more efficient for eliminating Pb from wastewater. The EDTA (0.1 gL<sup>−1</sup>) assisted <em>Pontederia crassipes</em> as a phytoremediation system enabled as high as 85% removal of lead from the studied effluent wastewater. This promises the use of this system for efficiently treating the effluent wastewater contaminated with lead. Tentative mechanisms of chelator-mediated metal ion transport and their detoxification within the plant cells are discussed.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102733"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Lee, O.J. Ojo-kupoluyi, S.M. Tahir, M.S. Anuar, S.U. Masuri, M.E. Ya'acob
{"title":"Microwave drying as an energy-efficient fuel pre-treatment and its cogeneration-level implications for sugarcane bagasse","authors":"Y. Lee, O.J. Ojo-kupoluyi, S.M. Tahir, M.S. Anuar, S.U. Masuri, M.E. Ya'acob","doi":"10.1016/j.biteb.2026.102752","DOIUrl":"10.1016/j.biteb.2026.102752","url":null,"abstract":"<div><div>High moisture content limits the efficient bioenergy utilization of sugarcane bagasse (SCB) by reducing its calorific value and cogeneration performance. This study systematically compares microwave and convective oven drying as pre-treatment strategies through controlled experiments conducted in triplicate under varying power levels (100–600 W), temperatures (50–110 °C), sample masses (1–5 g), and initial moisture contents (30–67%, wet basis). Drying kinetics, effective moisture diffusivity (<em>D</em><sub><em>eff</em></sub>), and drying efficiency were evaluated using Fick's second law and energy balance analysis. Microwave drying reduced moisture from 67% to equilibrium within ~4 min at 600 W, compared to ~45 min for oven drying at 110 °C. Microwave treatment achieved higher <em>D</em><sub><em>eff</em></sub> (1.53 × 10<sup>−7</sup>–3.88 × 10<sup>−7</sup> m<sup>2</sup>/s) and drying efficiency (up to 47.4%). The enhanced internal heating mechanism in microwave dryer significantly improved moisture migration and reduced drying time, demonstrating its potential for energy-efficient biomass pre-conditioning in cogeneration systems.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102752"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}