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A technique for the masses: single-cell analysis using mass cytometry. 面向大众的技术：利用质谱仪进行单细胞分析。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2024-02-01 Epub Date: 2024-01-08 DOI: 10.2144/btn-2023-0121
Beatrice Bowlby
{"title":"A technique for the masses: single-cell analysis using mass cytometry.","authors":"Beatrice Bowlby","doi":"10.2144/btn-2023-0121","DOIUrl":"10.2144/btn-2023-0121","url":null,"abstract":"<p><p>Mass cytometry, a technique that bridges the gap between flow cytometry and mass spectrometry, reveals how immune cell distribution differs in disease states, such as in the peripheral blood of coronary artery disease patients and the lesion microenvironment of endometriosis. [Formula: see text].</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"43-45"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports. 三维生物打印机的应用：在水凝胶支持物上使用细胞进行 "生物补丁 "开发的喷射技术。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2024-02-01 Epub Date: 2023-12-12 DOI: 10.2144/btn-2023-0052
Derek C DeMel, Grayson A Wagner, Jamie A Maresca, John P Geibel
{"title":"Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports.","authors":"Derek C DeMel, Grayson A Wagner, Jamie A Maresca, John P Geibel","doi":"10.2144/btn-2023-0052","DOIUrl":"10.2144/btn-2023-0052","url":null,"abstract":"<p><p>Additive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended periods. Combining a flexible biosupport with application of cells via TIPS printing now for the first time allows for custom cell substrate constructs with various densities to be deployed for regenerative medicine applications.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"52-62"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide. 硫化氢定量的比色、光谱和电化学技术比较。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2024-02-01 Epub Date: 2023-12-07 DOI: 10.2144/btn-2023-0075
Susmit Mhatre, Anjali Rai, Hatim Ali, Akash Patil, Neetu Singh, Richa Verma, John Auden, Cole Chandler, Alekha Dash, Catherine Opere, Somnath Singh
{"title":"Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide.","authors":"Susmit Mhatre, Anjali Rai, Hatim Ali, Akash Patil, Neetu Singh, Richa Verma, John Auden, Cole Chandler, Alekha Dash, Catherine Opere, Somnath Singh","doi":"10.2144/btn-2023-0075","DOIUrl":"10.2144/btn-2023-0075","url":null,"abstract":"<p><p><b>Background:</b> Hydrogen sulfide (H<sub>2</sub>S), an endogenous gasotransmitter, has potential applications in several conditions. However, its quantification in simulated physiological solutions is a major challenge due to its gaseous nature and other physicochemical properties. <b>Aim:</b> This study was designed to compare four commonly used H<sub>2</sub>S detection and quantification methods in aqueous solutions. <b>Methods:</b> The four techniques compared were one colorimetric, one chromatographic and two electrochemical methods. <b>Results:</b> Colorimetric and chromatographic methods quantified H<sub>2</sub>S in millimolar and micromole ranges, respectively. The electrochemical methods quantified H<sub>2</sub>S in the nanomole and picomole ranges and were less time-consuming. <b>Conclusion:</b> The H<sub>2</sub>S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"71-80"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138497750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The environmental impact of AI in the lab: a double-edged sword? 实验室人工智能对环境的影响：一把双刃剑？
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2024-07-31 DOI: 10.1080/07366205.2024.2376459
Annie Coulson
{"title":"The environmental impact of AI in the lab: a double-edged sword?","authors":"Annie Coulson","doi":"10.1080/07366205.2024.2376459","DOIUrl":"10.1080/07366205.2024.2376459","url":null,"abstract":"<p><p>Computational tools, particularly AI, are becoming more ubiquitous in scientific research; but what impact do they have on the environment?[Formula: see text].</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"353-356"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cancer's sweet spot: techniques to harness saccharides in tumor biology. 癌症的甜蜜点:在肿瘤生物学中利用糖类的技术。
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2023-12-04 DOI: 10.2144/btn-2023-0110
Jade Parker
{"title":"Cancer's sweet spot: techniques to harness saccharides in tumor biology.","authors":"Jade Parker","doi":"10.2144/btn-2023-0110","DOIUrl":"10.2144/btn-2023-0110","url":null,"abstract":"<p><p>All of the cells in our bodies are enveloped in sugar, this sweet coating plays a particularly interesting and crucial role in tumor biology. Here, we review the techniques being used to detect and exploit cancer's sweet spot. including click chemistry, glycoproteomic profiling and bioorthogonal chemistry.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"5-8"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138476703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution. 从 Triton X-100 过渡到 Tergitol 15-S-9：对使用病毒 PCR 样品溶液进行诊断测定的影响。
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2024-05-05 DOI: 10.1080/07366205.2024.2342172
Azul Zorzoli, Alasdair MacLean, Scott Nicholson, Alison Daniels, Stephen Hughes, Susan Bennet-Slater, Christine Tait-Burkard, Noha El Sakka, Rory Gunson, Kate Templeton
{"title":"Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution.","authors":"Azul Zorzoli, Alasdair MacLean, Scott Nicholson, Alison Daniels, Stephen Hughes, Susan Bennet-Slater, Christine Tait-Burkard, Noha El Sakka, Rory Gunson, Kate Templeton","doi":"10.1080/07366205.2024.2342172","DOIUrl":"10.1080/07366205.2024.2342172","url":null,"abstract":"<p><p>In 2019, the European Union banned Triton X-100, a detergent widely used in laboratory diagnostics, including the Viral PCR Sample Solution (VPSS), and urged manufacturers to find environmentally sustainable alternatives. Tergitol 15-S-9 (VPSS2) has been proposed as an alternative surfactant. This multicenter study evaluated the effectiveness of VPSS2, a Tergitol-based viral solution, as a replacement for VPSS. Our results show the equivalent performance of VPSS2 to VPSS for nucleic acid extraction and viral stability over time at different temperatures. The new VPSS formulation was also tested against external quality assurance panels and clinical samples. The results of this work support adopting this modified viral PCR sample solution to replace Triton X-100-containing viral transport solutions.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"295-298"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adipocyte ABCA1 expression analysis using flow cytometry. 使用流式细胞仪分析脂肪细胞 ABCA1 的表达。
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2024-07-17 DOI: 10.1080/07366205.2024.2376466
Sakshi Shukla, Ashutosh Bansal, Sandeep Aggarwal, Archna Singh
{"title":"Adipocyte ABCA1 expression analysis using flow cytometry.","authors":"Sakshi Shukla, Ashutosh Bansal, Sandeep Aggarwal, Archna Singh","doi":"10.1080/07366205.2024.2376466","DOIUrl":"10.1080/07366205.2024.2376466","url":null,"abstract":"<p><p>Adipocyte characterization and assessing membrane proteins using flow cytometry has been proven to be challenging as adipocytes are fragile, especially in subjects with high BMI. We overcame these challenges through a protocol optimizing tissue digestion time by reducing intermediate steps to minimize adipocyte friction and breakage. We avoided requirement for specialized instrument configuration and used a modified gating strategy to prevent inclusion of lipid droplets during analysis. Up to 90% of the cell population were available in the gating area. We checked the expression level of ABCA1, a membrane protein reaffirming adipocyte selection. In summary, this protocol requires lesser tissue sample improving feasibility and cost efficiency. Thus, our flow cytometry method is an improvement for studying adipocyte membrane characteristics.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"405-410"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle. 基于手枪式 DNA 酶介导的外切酶辅助信号循环的葡萄糖双模式检测。
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2024-08-05 DOI: 10.1080/07366205.2024.2381403
Huiying Lu, Xiaofeng Wang
{"title":"Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle.","authors":"Huiying Lu, Xiaofeng Wang","doi":"10.1080/07366205.2024.2381403","DOIUrl":"10.1080/07366205.2024.2381403","url":null,"abstract":"<p><p>Detecting glucose accurately and sensitively from clinical samples like tears and saliva is still difficult. We have created a sensor that can detect glucose with high sensitivity and accuracy by combining the use of glucose oxidase (GOx) to catalyze glucose, a pistol-like DNAzyme (PLDz) to transform the signal, gold nanoparticles (AuNPs) to enhance the optical properties and the exonuclease-III (Exo-III) to amplify the signal. As a result, the proposed method exhibits a low detection limit of 7.5 pM and a wide detection range covering seven orders of magnitude. The suggested dual-mode strategy provides a sensitive, precise and specific detection method for glucose. Another advantage is that the dual-mode technique significantly improves the precision and consistency of the measurements, demonstrating its immense potential for use in biomedical research and clinical diagnostics.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"415-423"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion. 动态二重奏：低温电子显微镜与机器学习相结合，实现生物分子运动的可视化。
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2024-06-12 DOI: 10.1080/07366205.2024.2355771
Beatrice Bowlby
{"title":"The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion.","authors":"Beatrice Bowlby","doi":"10.1080/07366205.2024.2355771","DOIUrl":"10.1080/07366205.2024.2355771","url":null,"abstract":"<p><p>Cryo-EM has been a key technique in our understanding of biomolecular structures. Now, machine learning techniques are being used to put these structures in motion, revealing dynamic interactions and processes happening on a molecular and cellular level.[Formula: see text].</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"229-233"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Artificial intelligence: help or hindrance in solving the reproducibility crisis? 人工智能：帮助还是阻碍解决可重复性危机？
IF 2.2 4区 工程技术
BioTechniques Pub Date : 2024-01-01 Epub Date: 2024-06-20 DOI: 10.1080/07366205.2024.2355776
Jennifer Straiton
{"title":"Artificial intelligence: help or hindrance in solving the reproducibility crisis?","authors":"Jennifer Straiton","doi":"10.1080/07366205.2024.2355776","DOIUrl":"10.1080/07366205.2024.2355776","url":null,"abstract":"<p><p>Science is in the midst of a reproducibility crisis and the integration of artificial intelligence into scientific research has only compounded the problem; yet could the technology hold the solution to its own problems?[Formula: see text].</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":" ","pages":"291-294"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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