发布求助
文献互助 智能选刊 最新文献

BioTechniques最新文献

筛选
英文 中文
Developing virtual and augmented reality applications for science, technology, engineering and math education. 开发用于科学、技术、工程和数学教育的虚拟和增强现实应用程序。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-07-01 Epub Date: 2023-06-09 DOI: 10.2144/btn-2023-0029
Christopher L Hemme, Rachel Carley, Arielle Norton, Moez Ghumman, Hannah Nguyen, Ryan Ivone, Jyothi U Menon, Jie Shen, Matthew Bertin, Roberta King, Elizabeth Leibovitz, Roy Bergstrom, Bongsup Cho
{"title":"Developing virtual and augmented reality applications for science, technology, engineering and math education.","authors":"Christopher L Hemme,&nbsp;Rachel Carley,&nbsp;Arielle Norton,&nbsp;Moez Ghumman,&nbsp;Hannah Nguyen,&nbsp;Ryan Ivone,&nbsp;Jyothi U Menon,&nbsp;Jie Shen,&nbsp;Matthew Bertin,&nbsp;Roberta King,&nbsp;Elizabeth Leibovitz,&nbsp;Roy Bergstrom,&nbsp;Bongsup Cho","doi":"10.2144/btn-2023-0029","DOIUrl":"10.2144/btn-2023-0029","url":null,"abstract":"<p><p>The Rhode Island IDeA Network of Biomedical Research Excellence Molecular Informatics Core at the University of Rhode Island Information Technology Services Innovative Learning Technologies developed virtual and augmented reality applications to teach concepts in biomedical science, including pharmacology, medicinal chemistry, cell culture and nanotechnology. The apps were developed as full virtual reality/augmented reality and 3D gaming versions, which do not require virtual reality headsets. Development challenges included creating intuitive user interfaces, text-to-voice functionality, visualization of molecules and implementing complex science concepts. In-app quizzes are used to assess the user's understanding of topics, and user feedback was collected for several apps to improve the experience. The apps were positively reviewed by users and are being implemented into the curriculum at the University of Rhode Island.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology. 利用探针改变链自组装反应技术对反义寡核苷酸进行灵敏和特异的定量分析。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-07-01 DOI: 10.2144/btn-2023-0005
Masako Osawa, Takurou Akiya, Funa Ogawa, Takao Suzuki, Masaki Yamagami, Tadashi Umemoto, Akira Ideno
{"title":"Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology.","authors":"Masako Osawa,&nbsp;Takurou Akiya,&nbsp;Funa Ogawa,&nbsp;Takao Suzuki,&nbsp;Masaki Yamagami,&nbsp;Tadashi Umemoto,&nbsp;Akira Ideno","doi":"10.2144/btn-2023-0005","DOIUrl":"https://doi.org/10.2144/btn-2023-0005","url":null,"abstract":"<p><p>Quantitative bioanalysis is essential when establishing pharmacokinetic properties during the drug development process. To overcome challenges of sensitivity, specificity and process complexity associated with the conventional analysis of antisense oligonucleotides (ASOs), a new approach to nonenzymatic hybridization assays using probe alteration-linked self-assembly reaction (PALSAR) technology as a signal amplifier was evaluated. PALSAR quantification of ASOs in mouse tissue and plasma was able to achieve a high sensitivity ranging from 1.5 to 6 pg/ml, intra-/interday accuracies in the range of 86.8-119.1% and 88.1-113.1%, respectively, and precision of ≤17.2%. Furthermore, crossreactivity of 3'n-1, a metabolite with a single base difference, was <1%. Our approach provides an auspicious method for distinguishing metabolites and detecting ASOs with high sensitivity and specificity.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9946132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial marvels: could microbes resolve climate change? 微生物奇迹:微生物能解决气候变化问题吗?
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-06-01 DOI: 10.2144/btn-2023-0043
Jade Parker
{"title":"Microbial marvels: could microbes resolve climate change?","authors":"Jade Parker","doi":"10.2144/btn-2023-0043","DOIUrl":"https://doi.org/10.2144/btn-2023-0043","url":null,"abstract":"<p><p>Our planet faces many challenges. Namely, the damage caused by human disruption of natural levels of carbon and nitrogen as a result of the use of fossil fuels. Microbes play a crucial role in the biogeochemical processing of these elements, in turn placing them at the heart of one of the most testing issues of all time - climate change. [Formula: see text].</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9927852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiscale computational modeling offers key to understanding molecular logic underpinning development and disease. 多尺度计算建模为了解发育和疾病的分子逻辑提供了关键。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-06-01 Epub Date: 2023-06-16 DOI: 10.2144/btn-2023-0039
Himanshu Kaul
{"title":"Multiscale computational modeling offers key to understanding molecular logic underpinning development and disease.","authors":"Himanshu Kaul","doi":"10.2144/btn-2023-0039","DOIUrl":"10.2144/btn-2023-0039","url":null,"abstract":"","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A modified method for efficient RNA isolation from mangrove root tissues rich in secondary metabolites. 从富含次生代谢物的红树林根组织中高效分离RNA的改进方法。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-06-01 DOI: 10.2144/btn-2022-0078
Ashifa Nizam, Haritha Kalath, Ajay Kumar
{"title":"A modified method for efficient RNA isolation from mangrove root tissues rich in secondary metabolites.","authors":"Ashifa Nizam,&nbsp;Haritha Kalath,&nbsp;Ajay Kumar","doi":"10.2144/btn-2022-0078","DOIUrl":"https://doi.org/10.2144/btn-2022-0078","url":null,"abstract":"<p><p>Secondary metabolites in mangroves often interfere with RNA extraction yielding poor concentration and quality, which is unsuitable for downstream applications. As existing protocols yielded low-quality RNA from root tissues of <i>Kandelia candel</i> (L.) Druce and <i>Rhizophora mucronata</i> Lam., an optimized method was developed for improving the quality and yield of RNA. Compared with three other methods, this optimized protocol gave better RNA yield and purity for both species. The absorbance ratios were ≥1.9 for A260/280 and A260/230, while RNA integrity number values ranged from 7.5 to 9.6. Results show that our modified method is efficient in obtaining high-quality RNA from mangrove roots and is suitable for downstream experiments such as cDNA synthesis, real-time quantitative PCR and next-generation sequencing.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PCR-based gene synthesis with overlapping unisense-oligomers asymmetric extension supported by a simulator for oligonucleotide extension achieved 1 kbp dsDNA. 基于聚合酶链反应的重叠寡核苷酸-寡聚物不对称延伸基因合成在寡核苷酸延伸模拟器的支持下获得了1 kbp的dsDNA。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-06-01 DOI: 10.2144/btn-2022-0127
Yasunori Nishida, Kotetsu Kayama, Taichi Endoh, Kiwamu Hanazono, Gerry Amor Camer, Daiji Endoh
{"title":"PCR-based gene synthesis with overlapping unisense-oligomers asymmetric extension supported by a simulator for oligonucleotide extension achieved 1 kbp dsDNA.","authors":"Yasunori Nishida,&nbsp;Kotetsu Kayama,&nbsp;Taichi Endoh,&nbsp;Kiwamu Hanazono,&nbsp;Gerry Amor Camer,&nbsp;Daiji Endoh","doi":"10.2144/btn-2022-0127","DOIUrl":"https://doi.org/10.2144/btn-2022-0127","url":null,"abstract":"<p><p>We formulated a method to synthesize 1 kbp DNA fragments using 'oligomer unidirectional joining method' via asymmetric extension supported by a simulator for oligonucleotide extension (AESOE). In this study, trials were conducted on 41 sets of different genomic pieces of ten flaviviral genomes, and 31 bacterial 16s rRNA fragments with sizes ranging from 500 bases to 1.0 kbp. Synthetic gene production was found to be successful in all those sets. The synthesis method has three steps: the first step is a seven-linked AESOE, the second step is the linking of the 400-base fragments from the first step, and the third step is the final amplification. Our present approach is highly reproducible and may no longer require optimization of oligomer design.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gibson assembly interposition improves amplification efficiency of long DNA and multifragment overlap extension PCR. 吉布森装配插入法提高了长DNA和多片段重叠延伸PCR的扩增效率。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-06-01 DOI: 10.2144/btn-2023-0012
Junyi Liu, Fangyin Liu, Xueer Luo, Ming Chen, Chengjun Wang, Liuyue Wang, Huabo Chen
{"title":"Gibson assembly interposition improves amplification efficiency of long DNA and multifragment overlap extension PCR.","authors":"Junyi Liu,&nbsp;Fangyin Liu,&nbsp;Xueer Luo,&nbsp;Ming Chen,&nbsp;Chengjun Wang,&nbsp;Liuyue Wang,&nbsp;Huabo Chen","doi":"10.2144/btn-2023-0012","DOIUrl":"https://doi.org/10.2144/btn-2023-0012","url":null,"abstract":"<p><p>For difficult overlap extension PCR, a Gibson assembly process was inserted between the two PCR rounds to facilitate the formation of complete gene templates at a moderate temperature. That is, after amplifying each DNA fragment, they were preluded by a Gibson assembly process in equal proportion. Then, the assembled mixture was used as a template for the second PCR round. This idea was tested and verified by taking the cloning example of a single and a double site mutation of the retinoblastoma gene. This scheme associates overlap extension PCR with Gibson assembly exquisitely, significantly improving gene amplification efficiency, particularly in the fusion of long genes and multifragments using overlap extension PCR.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10231372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct-current electric field stimulation promotes proliferation and maintains stemness of mesenchymal stem cells. 直流电刺激可促进间充质干细胞增殖，维持干细胞的干性。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-06-01 DOI: 10.2144/btn-2022-0112
Mengchang Liu, Defu Xie, Huizhen Zeng, Ning Zhai, Lan Liu, Hong Yan
{"title":"Direct-current electric field stimulation promotes proliferation and maintains stemness of mesenchymal stem cells.","authors":"Mengchang Liu,&nbsp;Defu Xie,&nbsp;Huizhen Zeng,&nbsp;Ning Zhai,&nbsp;Lan Liu,&nbsp;Hong Yan","doi":"10.2144/btn-2022-0112","DOIUrl":"https://doi.org/10.2144/btn-2022-0112","url":null,"abstract":"<p><p>Mesenchymal stem cells are frequently utilized in the study of regenerative medicine. Electric fields (EFs) influence many biological processes, such as cell proliferation, migration and differentiation. In the present study, a novel device capable of delivering a direct current of EF stimulation to cells cultured <i>in vitro</i> is described. This bioreactor was customized to simultaneously apply a direct-current EF to six individual cell culture wells, which reduces the amount of experimental time and minimizes cost. In testing the device, adipose-derived mesenchymal stem cells stimulated with an EF in the bioreactor exhibited a greater cell proliferation rate while retaining stemness. The results provide a unique perspective on adipose-derived mesenchymal stem cell proliferation, which is needed for tissue engineering and regenerative medicine.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The path to solving the protein folding problem. 解决蛋白质折叠问题的途径。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-05-01 DOI: 10.2144/btn-2023-0031
Jenny Straiton
{"title":"The path to solving the protein folding problem.","authors":"Jenny Straiton","doi":"10.2144/btn-2023-0031","DOIUrl":"https://doi.org/10.2144/btn-2023-0031","url":null,"abstract":"<p><p>[Formula: see text] With advances in imaging technologies and the development of artificial intelligence-based predictive software, has the protein folding problem finally been solved?</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10004374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target events. Target-seq:检测基因组整合位点、DNA易位和脱靶事件的单一工作流程。
IF 2.7 4区 工程技术
BioTechniques Pub Date : 2023-05-01 DOI: 10.2144/btn-2023-0013
Pei-Zhong Tang, Bo Ding, Christopher Reyes, David Papp, Jason Potter
{"title":"Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target events.","authors":"Pei-Zhong Tang,&nbsp;Bo Ding,&nbsp;Christopher Reyes,&nbsp;David Papp,&nbsp;Jason Potter","doi":"10.2144/btn-2023-0013","DOIUrl":"https://doi.org/10.2144/btn-2023-0013","url":null,"abstract":"<p><p>Designed donor DNA delivery through viral or nonviral systems to target loci in the host genome is a critical step for gene therapy. Adeno-associated virus and lentivirus are leading vehicles for <i>in vivo</i> and <i>ex vivo</i> delivery of therapeutic genes due to their high delivery and editing efficiency. Nonviral editing tools, such as CRISPR/Cas9, are getting more attention for gene modification. However, there are safety concerns; for example, tumorigenesis due to off-target effects and DNA rearrangement. Analysis tools to detect and characterize on-target and off-target genome modification post editing in the host genome are pivotal for evaluating the success and safety of gene therapy. We developed Target-seq combined with different analysis tools to detect the genome integration site, DNA translocation and off-target events.</p>","PeriodicalId":8945,"journal":{"name":"BioTechniques","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10002812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信