用于量化微型动物游泳行为的免费且用户友好的软件协议。

IF 2.2 4区 工程技术 Q3 BIOCHEMICAL RESEARCH METHODS
BioTechniques Pub Date : 2024-05-01 Epub Date: 2024-02-29 DOI:10.2144/btn-2024-0001
Colleen M McMaken, Kristin E Gribble
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引用次数: 0

摘要

在测量其他生物参数的同时,游泳行为的特征描述可以对微小动物的健康、生理和生态进行整体评估。然而,由于动物体型小或透明度低,或者由于分析所需的成本、专业知识或劳动力较高,使用现有的自动化工具跟踪和量化微型动物的游泳行为通常比较困难。为了解决这些问题,我们创建了一种成本效益高、用户友好的行为分析方案,该方案采用了免费的软件包 HitFilm 和 ToxTrac 以及 R 软件包 "trajr",并使用该方法量化了轮虫的行为。该方案可用于其他微动物物种,研究人员在获取游泳行为测量结果时可能会遇到类似的问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A free and user-friendly software protocol for the quantification of microfauna swimming behavior.

Characterizing swimming behavior can provide a holistic assessment of the health, physiology and ecology of microfaunal species when done in conjunction with measuring other biological parameters. However, tracking and quantifying microfauna swimming behavior using existing automated tools is often difficult due to the animals' small size or transparency, or because of the high cost, expertise, or labor needed for the analysis. To address these issues, we created a cost-effective, user-friendly protocol for behavior analysis that employs the free software packages HitFilm and ToxTrac along with the R package 'trajr' and used the method to quantify the behavior of rotifers. This protocol can be used for other microfaunal species for which investigators may face similar issues in obtaining measurements of swimming behavior.

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来源期刊
BioTechniques
BioTechniques 工程技术-生化研究方法
CiteScore
4.40
自引率
0.00%
发文量
68
审稿时长
3.3 months
期刊介绍: BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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