Biomedicines最新文献

{"title":"Retinal Thickness in Patients with Parkinson's Disease and Dopa Responsive Dystonia-Is There Any Difference?","authors":"Marko Svetel, Gorica Marić, Marija Božić, Una Lazić, Andona Milovanović, Jana Jakšić, Igor Petrović, Ana Dimitrijević, Milica Knežević, Tatjana Pekmezović","doi":"10.3390/biomedicines13051227","DOIUrl":"10.3390/biomedicines13051227","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Certain aspects of retinal thickness assessed by optical coherence tomography (OCT) in patients with Parkinson's disease (PD) require additional clarification. It is supposed that attributing reduced retinal thickness in PD to dopaminergic loss may not be acceptable as it also happens in diseases where dopaminergic loss does not occur. The objective of our study is to compare the ganglion cell/inner plexiform layer (GCIPL), peripapillary retinal nerve fiber layer (pRNFL), and macular thickness of PD and dopa responsive dystonia (DRD) patients with healthy controls (HC), to investigate whether DRD patients, as a distinctive model of genetically induced dopamine deficiency, have reduced retinal thickness in comparison with PD, and to analyze correlation between retinal thickness and various PD clinical parameters. <b>Methods</b>: We analyzed 86 patients with PD, 10 patients with DRD, and 96 age- and sex-matched HC. <b>Results</b>: GCIPL, pRNFL, and central macula thickness (CMT) are statistically significantly thinner in PD patients compared to HC (<i>p</i> < 0.001, all). GCIPL and CMT are also statistically significantly thinner in DRD patients compared to HC (<i>p</i> = 0.012, <i>p</i> = 0.001, respectively). GCIPL thickness correlates positively with the daily dose of levodopa (r = 0.244, <i>p</i> < 0.01). The thickness of GCIPL and pRNFL correlate negatively with current age (r = -0.219; <i>p</i> < 0.01 and r = -0.358; <i>p</i> < 0.05, respectively). All retinal parameters are statistically significantly thinner in females than in males (<i>p</i> < 0.05). <b>Conclusions</b>: Patients with PD and DRD did not differ in GCIPL and pRNFL thickness when compared to one another. These results, supported by positive correlation of levodopa dose and GCIPL thickness in PD patients, emphasize the importance of dopamine in maintaining retinal thickness.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Viral and Bacterial Co-Infections in SARS-CoV-2-Positive Individuals in Cyprus 2020-2022.","authors":"George Krashias, Christina Tryfonos, Stavros Bashiardes, Jan Richter, Christina Christodoulou","doi":"10.3390/biomedicines13051236","DOIUrl":"10.3390/biomedicines13051236","url":null,"abstract":"<p><p>The COVID-19 pandemic has had a profound impact on healthcare systems worldwide, with severe consequences on the global economy and society. The clinical presentation of SARS-CoV-2 infection varies widely, ranging from asymptomatic cases to severe disease and death. Coinfection with other respiratory pathogens in SARS-CoV-2-positive individuals may exacerbate symptom severity and lead to poorer clinical outcomes. <b>Background/Objectives</b>: This study is the first to investigate the prevalence of viral and bacterial co-infections in SARS-CoV-2-positive individuals in Cyprus. <b>Methods</b>: A total of 1111 SARS-CoV-2-positive nasopharyngeal swab samples collected from non-hospitalized patients were analyzed for the presence of 18 viral and 3 bacterial respiratory pathogens. <b>Results</b>: Of these, 51 samples (4.6%) were found to have at least one additional respiratory pathogen. The most frequently detected viruses were rhinovirus/enterovirus (<i>n</i> = 28; 2.5%) and adenovirus (<i>n</i> = 8; 0.7%), while the bacterial pathogens identified were <i>Legionella pneumophila</i> (<i>n</i> = 1; 0.1%) and <i>Bordetella pertussis</i> (<i>n</i> = 1; 0.1%). The highest proportion of co-infections was observed in the youngest age group (<10 years), where 52.9% of co-infections were identified, followed by the 30-39 age group, which accounted for 15.7% of cases. Among single respiratory virus co-infections, rhinovirus/enterovirus (27.5%) and adenovirus (13.7%) were the most frequently detected in the <10 age group, followed by RSV (3.9%), bocavirus, influenza B, HMPV A + B, and coronavirus NL63 (each at 2%). <b>Conclusions</b>: The current study underscores the importance of simultaneous testing for SARS-CoV-2 and other respiratory pathogens, as this may have significant implications for both individual patient care and healthcare services.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound Predictors for Persistence or a Change in the Diagnosis of Rheumatoid Arthritis After 5 Years-A Prospective Cohort Study of Patients with Early Rheumatoid Arthritis.","authors":"Tanya Sapundzhieva, Lyubomir Sapundzhiev, Martin Mitev, Rositsa Karalilova, Anastas Batalov","doi":"10.3390/biomedicines13051226","DOIUrl":"10.3390/biomedicines13051226","url":null,"abstract":"<p><p><b>Aim:</b> To identify ultrasound (US) predictors of persistence or change in the diagnosis of rheumatoid arthritis (RA) after five years in a cohort of patients with early RA. <b>Patients and Methods:</b> One hundred and twenty patients with early arthritis who met the 2010 ACR/EULAR classification criteria for RA were followed for a period of five years. The US assessment at baseline included a bilateral evaluation of the wrists, second to fifth metacarpophalangeal (MCP) joints, second to fifth proximal interphalangeal (PIP) joints, the IV and VI extensor compartments of the wrists, and the flexor tendons of the second to fifth fingers. This evaluation was conducted using both grayscale ultrasound (GSUS) and power Doppler ultrasound (PDUS). The following scores were recorded for each patient: synovitis score, mini-enthesitis score (including paratenonitis of the finger extensor tendon at the MCP joint, central slip enthesitis at the PIP joint, pseudotenosynovitis, and the A1 pulley of the second finger), finger flexor tenosynovitis score, and tenosynovitis score for the IV and VI wrist extensor compartments. The receiver operating characteristic (ROC) curve was utilized to identify the ultrasound predictors for either maintaining or revising an initial diagnosis of RA. <b>Results:</b> At month 6, 82 (68%) patients were classified as having RA according to 1987 ACR RA criteria, 23 (19.2%) were diagnosed with psoriatic arthritis (PsA), 10 (8.3%) with systemic connective tissue disease (SCTD)-8 (6.7%) patients with Sjogren Syndrome and 2 (1.7%) patients with systemic lupus erythematosus (SLE)-and 5 (4.2%) patients with calcium pyrophosphate deposition disease (CPPD). The most significant predictor of RA in the fifth year was the VI extensor compartment tenosynovitis score, with an AUC of 0.915 and a criterion value > 0, associated with a sensitivity of 82.93% and a specificity of 100% (<i>p</i> < 0.001). The PDUS synovitis score demonstrated the second-best prognostic ability with an AUC of 0.823, a criterion value > 2, a sensitivity of 82.93%, and a specificity of 73.68% (<i>p</i> < 0.001). The mini-enthesitis score showed the best prognostic ability of a PsA diagnosis with an AUC of 0.998, a criterion value > 1, a sensitivity of 95.65%, and a specificity of 100% (<i>p</i> < 0.001). The paratenonitis score, pseudotenosynovitis score, and thickened A1 pulley were also predictive of PsA diagnosis with AUCs of 0.977, 0.955, and 0.919, respectively (<i>p</i> < 0.001 for all). <b>Conclusions:</b> Nearly one-third of the patients who were initially classified as having RA had their diagnosis revised at the end of the fifth year. Ultrasound of joints, tendons, and mini-entheses at baseline may serve as potential imaging predictive biomarkers for persistence or change in diagnosis after 5 years.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Placenta Hydrolysate Protects Against Acetaminophen-Induced Liver Injury in Mice.","authors":"Inyoung Hwang, Chi-Gu Kang, So-Jung Lim, Hyun-Jin Kim, Ryun Kang, So-Hyun Jeon, Sang-Hoon Lee, Jae-Won Kim, Ju-Seop Kang","doi":"10.3390/biomedicines13051219","DOIUrl":"10.3390/biomedicines13051219","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Acetaminophen (APAP) is a widely used analgesic and antipyretic, but overdose can lead to APAP-induced liver injury (AILI), a major cause of acute liver failure. While N-acetylcysteine (NAC) is the current standard of care, its efficacy is significantly reduced when administered after the peak time of liver injury, highlighting the need for alternative therapeutic strategies. Human placenta hydrolysate (HPH) has shown potential as a therapeutic agent for various liver diseases due to its rich content of bioactive compounds. This study aimed to investigate the hepatoprotective effects of HPH in a mouse model of AILI. <b>Methods</b>: HPH was administered to mice for three days prior to APAP treatment. The effects of HPH on liver morphology, necrosis, liver enzymes, phase I/II detoxification enzymes, oxidative stress markers, and inflammatory cytokines were evaluated. <b>Results</b>: HPH pretreatment attenuated APAP-induced liver necrosis and congestion, reduced serum levels of liver enzymes. In addition, HPH showed a concentration-dependent attenuation of APAP-induced decrease in human hepatocyte viability. HPH modulated phase I/II enzyme expression by downregulating CYP2E1 and upregulating SULT1A1, UGT1A6, GSTP1, and TPMT. HPH also exhibited antioxidant effects by increasing SOD and GPx activities, reducing MDA levels, and restoring the GSH/GSSG ratio. Furthermore, HPH attenuated the APAP-induced increase in the inflammatory cytokines TNF-α and IL-6. These findings suggest that HPH protects against AILI through multiple mechanisms, including the modulation of phase I/II detoxification, activation of antioxidants, and inhibition of inflammation. <b>Conclusions</b>: HPH could be a potential therapeutic option for APAP overdose and related liver injuries.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the Treatment of Acute Myeloid Leukemia: Implications for Low- and Middle-Income Countries.","authors":"Michelle Morcos-Sandino, Sofia Isabel Quezada-Ramírez, Andrés Gómez-De León","doi":"10.3390/biomedicines13051221","DOIUrl":"10.3390/biomedicines13051221","url":null,"abstract":"<p><p>Acute myeloid leukemia (AML) presents a significant global health challenge due to its aggressive behavior and mortality rates. Traditionally, AML treatment has relied on intensive chemotherapy-anthracyclines and cytarabine. However, recent breakthroughs in targeted therapies are transforming clinical practices. This review examines current treatment strategies, including breakthrough therapies. Also, a global perspective on AML management includes the disparity in treatment availability, particularly the difficulties faced by low- and middle-income countries due to the high cost and restricted access to novel therapies.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Analysis of <i>CYP1B1</i> and Other Anterior Segment Dysgenesis-Associated Genes in Latvian Cohort of Primary Congenital Glaucoma.","authors":"Eva Elksne, Baiba Lace, Janis Stavusis, Anastasija Tvoronovica, Pawel Zayakin, Eriks Elksnis, Arturs Ozolins, Ieva Micule, Sandra Valeina, Inna Inashkina","doi":"10.3390/biomedicines13051222","DOIUrl":"10.3390/biomedicines13051222","url":null,"abstract":"<p><p><b>Background</b>: Primary congenital glaucoma (PCG) is a rare disease with an incidence of 1 in 12,000 to 18,000 in Europeans. The scarcity of the disease and limited access to genetic testing have hindered research, particularly within the Latvian population. <b>Objectives</b>: This study aims to present the preliminary results of a molecular genetic investigation into PCG in a Latvian cohort and to compare the prevalence of gene <i>CYP1B1</i> variants with other European studies as well as to the general population in Latvia. <b>Methods</b>: Twenty probands with clinically diagnosed PCG and 36 family members enrolled in the study. Genetic testing was conducted using genomic DNA from peripheral blood using next generation sequencing (NGS) of seven selected genes: <i>CYP1B1, FOXC1, FOXE3, PXDN, PITX2, PITX3, PAX6</i>, and <i>CPAMD8</i>. Four probands had whole-genome sequencing (WGS). <b>Results</b>: All participants were of European ancestry, with no family history of PCG. Most probands were diagnosed in their first year of life, with a female to male ratio of 1:1.2 and with 80.0% of cases being unilateral. No <i>CYP1B1</i> pathogenic variants were identified in the screened subjects. However, a heterozygous missense variant c.4357C>A (p.Pro4357Thr) in the <i>PXDN</i> gene was found in one proband and one of her parents that was classified as a variant of uncertain significance. <b>Conclusions</b>: This study represents the first genetic characterization of PCG in the Latvian population. Using NGS, we identified no pathogenic variants in the <i>CYP1B1</i> gene among affected individuals. Preliminary evidence from this cohort does not support <i>CYP1B1</i> variants as a predominant cause of PCG, though larger studies are needed to confirm this observation. Comprehensive genetic screening using whole-exome or whole-genome sequencing will be essential to identify the underlying genetic etiology of PCG in Latvia.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palm Tocotrienol Preserves Trabecular Osteocyte Indices and Modulates the Expression of Osteocyte Markers in Ovariectomized Rats.","authors":"Sophia Ogechi Ekeuku, Shafiq Zikry Zarir, Anis Nazira Razali, Syamima Mohamad Zaidi, Noor Halinah Mohamed Ali Jinnah, Muhamed Lahtif Nor Muhamad, Sok Kuan Wong, Kok-Yong Chin","doi":"10.3390/biomedicines13051220","DOIUrl":"10.3390/biomedicines13051220","url":null,"abstract":"<p><p><b>Background/Objective:</b> Palm tocotrienol has bone-protective properties in animal models, yet its underlying mechanism remains unclear. Given osteocytes' role in bone homeostasis, this research aimed to investigate the effects of palm tocotrienol on the quantity of osteocytes and the expression of osteocyte-specific markers in ovariectomized rats. <b>Methods:</b> Adult female rats (Sprague Dawley; three-month-old; <i>n</i> = 6/group) were randomly divided into baseline, sham control, ovariectomized control, unemulsified palm tocotrienol (UPT), emulsified palm tocotrienol (EPT), and positive control. The baseline group was euthanized without intervention, whereas the sham group underwent a laparotomy procedure in which the ovaries were not excised. The other groups underwent bilateral removal of the ovaries and subsequently received UPT (100 mg/kg/day, 50% vitamin E), EPT (100 mg/kg/day, 25% vitamin E), or a combination of glucosamine sulfate (250 mg/kg/day) and calcium carbonate (1% in drinking water). Control groups were induced with similar gavage stress with olive oil. After 10 weeks, all rats were sacrificed for bone and serum analysis. <b>Results:</b> UPT and EPT significantly increased trabecular osteocyte and total lacunae numbers (<i>p</i> < 0.05 versus ovariectomized control). Both treatments significantly reduced mRNA expression levels of dentin matrix protein-1 (<i>p</i> < 0.05 versus ovariectomized control), whereas sclerostin mRNA expression was unchanged (<i>p</i> > 0.05 versus ovariectomized control). However, neither UPT nor EPT improved circulating or skeletal redox status (<i>p</i> > 0.05 versus ovariectomized control). <b>Conclusions:</b> Palm tocotrienol may support bone health by preserving the quantity of trabecular osteocytes and modulating osteocyte-mediated bone remodeling. Further research is required to elucidate its precise mechanisms.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended Toxicity, Genotoxicity, and Mutagenicity of Combination of pBudK-coVEGF-coANG and pBudK-coGDNF Plasmids in Preclinical Trials.","authors":"Igor V Samatoshenkov, Alexander M Aimaletdinov, Elena Y Zakirova, Egan L Kalmykov, Rustam Khodzhibaev, Yulia M Samatoshenkova, Ilnur M Ganiev, Marat S Kadyrov, Yana O Mukhamedshina","doi":"10.3390/biomedicines13051223","DOIUrl":"10.3390/biomedicines13051223","url":null,"abstract":"<p><p>Chronic lower limb ischemia is a debilitating condition, particularly prevalent among elderly patients and individuals ineligible for revascularization procedures. Gene therapy aimed at promoting therapeutic angiogenesis presents a promising alternative treatment strategy. <b>Objectives:</b> This study evaluated the preclinical safety of a gene therapy drug composed of the plasmids pBudK-coVEGF-coANG and pBudK-coGDNF in laboratory animals. Safety assessment followed a single intramuscular injection at a dose 30 times higher than the proposed therapeutic level. <b>Methods:</b> Acute toxicity was monitored over a 24-h period. Genotoxicity was assessed using the micronucleus test at doses of 200, 1000, and 5000 μg/kg. Bone marrow cytology was analyzed to detect hematopoietic toxicity. Delayed toxicity was evaluated over a two-week recovery period. <b>Results:</b> No signs of acute toxicity were observed, even at the highest dose. The micronucleus test revealed no genotoxic effects, with no significant increase in micronucleated polychromatic erythrocytes compared to control groups. Bone marrow erythroblast parameters remained within normal physiological ranges. Additionally, no delayed adverse effects were detected during the recovery period. <b>Conclusions:</b> The gene therapy drug demonstrated a favorable preclinical safety profile, exhibiting no evidence of toxicity or genotoxicity, even at substantially elevated doses. These findings support the continued development of this therapy as a potential treatment for chronic lower limb ischemia in patients who are not candidates for surgical intervention.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human and Mouse Bone Marrow CD45⁺ Erythroid Cells Have a Constitutive Expression of Antibacterial Immune Response Signature Genes.","authors":"Roman Perik-Zavodskii, Olga Perik-Zavodskaia, Julia Shevchenko, Kirill Nazarov, Anastasia Gizbrekht, Saleh Alrhmoun, Vera Denisova, Sergey Sennikov","doi":"10.3390/biomedicines13051218","DOIUrl":"10.3390/biomedicines13051218","url":null,"abstract":"<p><p><b>Introduction</b>: Recent studies have shown that Erythroid progenitor cells exhibit a distinct immunosuppressive and immunoregulatory phenotype associated with the response to bacteria. <b>Methods</b>: The objective of this study was to comprehensively explore the traits of human bone marrow Erythroid cells through protein-protein interaction network analysis using cytokine secretion analysis, and single-cell immunoproteomic analysis using flow cytometry, as well as the re-analysis of publicly available human and mouse bone marrow Erythroid-cell transcriptomic data. <b>Results</b>: Our protein-protein interaction network analysis of human bone marrow Erythroid-cell protein-coding genes identified enrichment in the immune response to lipopolysaccharide, with Calprotectin and Cathepsin G being the main factors. We then mapped the Calprotectin to the CD45⁺ Erythroid cells of both humans and mice via the analysis of the publicly available scRNA-seq data. Additionally, we observed that human bone marrow Erythroid cells secrete cytokines and chemokines, such as IL-1b, IL-8, and IL-18, which are also mainly involved in the immune response to lipopolysaccharide. We also found that human and mouse bone marrow Erythroid-cell conditional media inhibit bacterial growth in vitro. <b>Discussion</b>: These findings suggest that both human and mouse bone marrow CD45⁺ Erythroid cells possess the potential to combat pathogenic microbes and thus play a role in innate antimicrobial immunity. <b>Conclusions</b>: CD45⁺ Erythroid cells are a potent immunoregulatory cell population in both humans and mice.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Expression and Molecular Roles of MAMDC2 in MSS Colorectal Cancer with a High Tumor Stromal Ratio.","authors":"Yiling Liu, Shengnan Qian, Jia Wei, Jianting He, Minghui Li, Xiaobing Gao, Hong Cai, Yiqing Wang, Yue Han, Tianyuan Tan, Minhui Yang","doi":"10.3390/biomedicines13051217","DOIUrl":"10.3390/biomedicines13051217","url":null,"abstract":"<p><p><b>Background:</b> Colorectal cancer (CRC) heterogeneity is strongly influenced by molecular subtypes and tumor stroma interactions. The meprin/A5/PTPmu (MAM) domain, a conserved structural motif in transmembrane proteins, remains undercharacterized in CRC pathogenesis. <b>Methods:</b> We analyzed RNA-seq data from TCGA-COAD to evaluate MAM domain gene expression. Immunohistochemistry and Western blotting were conducted to validate the results of the database analysis. <b>Results:</b> Bioinformatics analysis revealed that MAM domain-containing protein 2 (MAMDC2) was enriched in mesenchymal subtype 4 (CMS4) colorectal cancer (<i>p</i> < 0.001). IHC confirmed MAMDC2 overexpression in MSS colorectal cancer with a high tumor stroma ratio (TSR) and peritoneal metastatic lesions (<i>p</i> < 0.01). WB and real-time PCR analyses confirmed that MAMDC2 has a role in regulating epithelial-mesenchymal transition (EMT) development in CRC. Importantly, we identified that cancer cell-derived MAMDC2 promotes MYLK expression in cancer-associated fibroblasts (CAFs) through paracrine signaling. <b>Conclusions:</b> Our findings suggest MAMDC2 may function as a stromal-associated regulator in MSS colorectal cancer with a high tumor stromal ratio (TSR).</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}