Biomedicines最新文献

{"title":"High PEEP Increases Airway Dead Space and Decreases Alveolar Ventilation: A New Technique for Volumetric Capnography.","authors":"Masashi Zuiki, Kazunori Watanabe, Norihiro Iwata, Rika Mitsuno, Madoka Konishi, Akio Yamano, Eisuke Ichise, Hidechika Morimoto, Kanae Hashiguchi, Tatsuji Hasegawa, Tomoko Iehara","doi":"10.3390/biomedicines13092275","DOIUrl":"10.3390/biomedicines13092275","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Identifying the optimal positive end-expiratory pressure (PEEP) is a major challenge in implementing strategies to prevent ventilator-induced lung injury in newborns. In this study, we assessed the validity of volumetric capnography based on the neonatal patient monitor (V_cap,PM) technique and investigated the impact of PEEP on newborns. <b>Methods:</b> Analysis 1 evaluated the validity of the V_cap,PM technique with data from pediatric patients receiving invasive respiratory support. Linear regression and Bland-Altman analyses were performed on V_cap,PM and HAMILTON-C1 data. Analysis 2 evaluated the impact of PEEP on newborns. The PEEP level was increased from mild to high (the incremental phase) and then decreased from high to mild (the decremental phase) while performing the V_cap,PM technique on term and preterm infants. <b>Results:</b> Analysis 1 included 31 children (age, 9 [interquartile range (IQR), 0-36] months; weight, 6.0 [IQR, 3.8-10.5] kg). Regression and Bland-Altman analyses demonstrated the accuracy of V_cap,PM. Analysis 2 included 28 term (mean gestational age, 38 [IQR, 38-40] weeks; weight, 2924 [IQR, 2725-3109] g) and 21 preterm (mean gestational age, 33 [IQR, 31-34] weeks; weight, 1918 [IQR, 1356-2186] g) newborns. Despite no difference in tidal volume, high PEEP significantly increased airway dead space and decreased alveolar tidal volume compared to mild PEEP in each phase in term and preterm neonates. <b>Conclusions:</b> High PEEP induced airway dilation in newborns, as determined using a novel V_cap technique. This technique, which requires no special equipment, has the potential for wider clinical application in neonatal care.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Pulmonary Hypertension on Hospitalization Risk in Adults with Respiratory Syncytial Virus Infection.","authors":"Mayuri Mudgal, Aseem Rai Bhatnagar, Aneesh Kumar Vasudevan, Ajeetha Priya Gajendiran, Venkatesh Gondhi, Swetha Balaji, Shanjai Krishnan Murugan, Kulothungan Gunasekaran","doi":"10.3390/biomedicines13092272","DOIUrl":"10.3390/biomedicines13092272","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Respiratory syncytial virus (RSV) infection can lead to significant complications, particularly among those with underlying cardiovascular and pulmonary complications. Patients with pulmonary hypertension (PH) are susceptible to clinical deterioration triggered by respiratory infections due to their limited cardiopulmonary reserve. This study aimed to assess the risk of hospitalization in RSV-infected adults with and without PH. <b>Methods</b>: We conducted a retrospective cohort study using the research network TriNetX to assess the risk of hospitalization in a cohort of patients with RSV infection, comparing those with and without PH. Propensity score matching was performed for demographic variables and RSV risk factors between the two cohorts. The risk of hospitalization was expressed as an adjusted odds ratio (aOR) with a 95% confidence interval (CI). <b>Results</b>: There were 193,256 patients in the RSV with PH cohort and 2,843,714 in the RSV without PH cohort (all aged >18 years). The mean age of the RSV with PH cohort was 68.2 ± 15.3 years, 50.6% were females, 64% were white, and 64.2% were group 2 PH. The RSV with PH cohort was at an increased risk of hospitalization (aOR 1.89, 95% CI 1.87-1.92, <i>p</i>-value 0.02). There was a significant risk (aOR 1.29, 95% CI 1.27-1.32) for the composite outcome of hospitalization-related complications between the two cohorts. Comorbid conditions (diabetes, cardiovascular disease, chronic lung disease, and chronic kidney disease) increased the risk of hospitalization in the RSV with PH group, with the biggest effect noted with underlying cardiovascular disease. Similarly, those with group 2 PH had a higher risk of hospitalization compared to the other PH groups. Remarkably, all PH groups demonstrated increased hospitalization risk compared to the RSV without PH cohort. <b>Conclusions</b>: We found that patients >18 years of age with PH and RSV infection were at an increased risk of hospitalization, with subsequently higher rates of RSV-infection-related complications. All PH groups had a higher hospitalization risk compared to the RSV without PH cohort, likely denoting PH as an independent risk factor for worse RSV-infection-related outcomes. RSV vaccination, therefore, may benefit all age groups of patients with PH.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>ABCA1</i>, <i>ADIPOQ</i>, <i>APOE</i>, <i>FSTL4</i>, and <i>KCNQ1</i> Gene DNA Methylation Correlates with Lipid Profiles in Mexican Populations.","authors":"Karla E Tello-Ortega, María A Romero-Tlalolini, Angélica Martínez-Hernández, Elizabeth Ortiz-Sánchez, Cecilia Contreras-Cubas, Humberto García-Ortiz, Francisco Barajas-Olmos, Lorena Orozco, Federico Centeno","doi":"10.3390/biomedicines13092273","DOIUrl":"10.3390/biomedicines13092273","url":null,"abstract":"<p><p><b>Background</b>: Dyslipidemia, a significant modifiable risk factor for cardiovascular disease (CVD), represents a major global health challenge, particularly influenced by complex genetic and environmental interactions, mainly in indigenous populations. <b>Methods:</b> In this study, DNA samples from 80 individuals belonging to various indigenous ethnic groups from northern and southern Mexico were analyzed to evaluate DNA methylation profiles and its correlation to lipid levels and other clinical parameters. Ten genes associated with metabolic changes were investigated using targeted bisulfite sequencing. <b>Results:</b> Our results revealed significant correlations between methylation in genes such as <i>ABCA1</i>, <i>ADIPOQ</i>, <i>APOE</i>, <i>FSTL4</i>, and <i>KCNQ1</i> and clinical parameters including body mass index (BMI), lipid profiles, and body fat. Of the 151 CpG sites analyzed, 16 showed statistically significant correlations. Specifically, two <i>ABCA1</i> CpGs sites correlated with BMI (<i>p</i> = 0.015) and triglycerides (<i>p</i> = 0.03); three <i>ADIPOQ</i> sites correlated with low-density lipoprotein cholesterol (LDLc) (<i>p</i> = 0.03, <i>p</i> = 0.005, <i>p</i> = 0.04, respectively); one <i>APOE</i> site correlated with BMI (<i>p</i> = 0.04), another with total cholesterol (<i>p</i> = 0.004) and triglycerides (<i>p</i> = 0.03) and two more with high-density lipoprotein cholesterol (HDLc) (<i>p</i> = 0.02 and <i>p</i> = 0.005, respectively); one <i>FSTL4</i> CpG site with body fat (<i>p</i> = 0.02), another with total cholesterol (<i>p</i> = 0.02), one more with HDLc (<i>p</i> = 0.01), and another one with triglycerides (<i>p</i> = 0.01); and two <i>KCNQ1</i> CpG sites correlated with body fat (<i>p</i> = 0.01 and <i>p</i> = 0.04, respectively). <b>Conclusions</b>: These findings show potential novel biomarkers for dyslipidemia risk. This research highlights the importance of understanding methylation changes in indigenous populations for developing personalized interventions and prevention strategies that could improve healthcare by linking epigenetic factors to CVD risk.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Progress in Keloid Mechanism and Treatment: A Comprehensive Review.","authors":"Lucia Merlino, Mattia Dominoni, Martina Rita Pano, Marianna Francesca Pasquali, Roberto Senatori, Grazia Zino, Barbara Gardella","doi":"10.3390/biomedicines13092276","DOIUrl":"10.3390/biomedicines13092276","url":null,"abstract":"<p><p>Keloids are abnormal fibroproliferative responses in the skin that often occur without an apparent injury. Their pathogenesis remains incompletely understood, though genetic, environmental, and biochemical factors are believed to contribute. Topical medications (mostly TCA injection) are the most used treatments followed by surgery, alone or in association with other therapeutic options. In most cases, improvement has been described. A combination of altered collagen synthesis, overactive fibroblasts, and immune response contributes to keloid formation. Genomic studies have identified specific mutations, and the role of growth factors such as TGF-β has been confirmed as a key player in keloid pathogenesis. Although great improvements have been made from the molecular point of view and keloids are more easily diagnosed and treatable nowadays, they remain very challenging, having a great impact on quality of life. Their recurrence is still very high. Understanding genetic predisposition and microenvironmental influences is critical for developing more effective therapies. Advances in molecular research and clinical strategies are improving our understanding of keloids, but further studies are needed to establish precise diagnostic markers and more effective long-term treatments.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Lee et al. Tumor-Associated Macrophages Affect the Tumor Microenvironment and Radioresistance via the Upregulation of CXCL6/CXCR2 in Hepatocellular Carcinoma. <i>Biomedicines</i> 2023, <i>11</i>, 2081.","authors":"Hsin-Lun Lee, Yi-Chieh Tsai, Narpati Wesa Pikatan, Chi-Tai Yeh, Vijesh Kumar Yadav, Ming-Yao Chen, Jo-Ting Tsai","doi":"10.3390/biomedicines13092271","DOIUrl":"10.3390/biomedicines13092271","url":null,"abstract":"<p><p>In the original publication [...].</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmaco-Epigenetics and Epigenetic Drugs in Type 2 Diabetes: Can Epigenetics Predict Drug Efficiency?","authors":"Senzosenkosi Surprise Mkhize, Anil Amichund Chuturgoon, Terisha Ghazi, Kgothatso Eugene Machaba","doi":"10.3390/biomedicines13092278","DOIUrl":"10.3390/biomedicines13092278","url":null,"abstract":"<p><p>Type 2 Diabetes Mellitus (T2DM) is increasingly affecting individuals across various age groups due to inadequate insulin action and secretion. It has become the leading cause of mortality worldwide, with an estimated 9.3% of the global population currently affected. Recent epigenetic studies have shown that variations such as DNA methylation and histone modifications are implicated in the development of T2DM. However, epigenetically related conditions are known to be reversible, which could potentially pave the way for predicting and treating T2DM. This has led to the development of epigenetic modifier drugs, including histone deacetylase inhibitors (HDACi), histone acetyltransferase inhibitors (HATi), protein arginine methyltransferase inhibitors (PRMTi), DNA methyltransferase inhibitors (DNMTi), histone demethylating inhibitors (HDMi), and sirtuin-activating compounds (STAC). A major challenge with these epigenetic drugs is that only a few have been approved for treating metabolic diseases due to their potential to negatively impact off-target genes. The low specificity of these drugs can lead to side effects and increased toxicity, contributing to complex diseases such as cancer. Hence, gaining a comprehensive understanding of the epigenetic mechanisms underlying metabolic diseases can provide new insights and strategies for preventing, diagnosing, and treating metabolic disorders, such as T2DM. This review summarizes the epigenetic variations in T2DM, pharmaco-epigenetics, and the challenges surrounding epigenetics. This provides basic insight into the discovery of novel drug targets, which can lead to the development of epigenetic therapies for T2DM. Hence, the reversible nature of epigenetic variations retains hope for future novel strategies to combat T2DM.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Proteins at Neuromuscular Junction Are Downgraded While NRG1 and Agrin Gene Expression Increases After Muscle Injury.","authors":"Jurandyr Pimentel Neto, Lara Caetano Rocha-Braga, Matheus Bertanha Fior, Paula Oliveira Camargo, Adriano Polican Ciena","doi":"10.3390/biomedicines13092277","DOIUrl":"10.3390/biomedicines13092277","url":null,"abstract":"<p><p><b>Background/Objectives:</b> The neuromuscular junction (NMJ) is the area where peripheral nerves communicate with muscle tissue. Muscle injury can occur as part of an acute degenerative process at the NMJ. This study aims to investigate the remodeling of the NMJ after a muscle injury in an experimental model. <b>Methods:</b> We used sixty male Wistar rats divided into five groups: a control group (C) and four muscle injury groups (MI) at different time points: 0 h, 24 h, 48 h, and 7 d after injury. We subjected the right hind limb to muscle injury and dissected the gastrocnemius muscles for analysis. We employed light microscopy to examine cell nuclei and the connective tissue, immunostaining to identify and measure the pre- and postsynaptic regions as well as calcium channels (P/Q), and real-time PCR to assess the gene expression of NRG1 and Agrin. <b>Results:</b> Our findings revealed an accumulation of nuclei and connective tissue in the acute injury groups (0 to 48 h). The morpho-quantitative analyses showed that the presynaptic structures and calcium channels underwent similar remodeling due to their morpho-functional relationship. Meanwhile, the postsynaptic receptors were significantly affected by the degenerative and inflammatory processes. These results can be linked to increased expression of NRG1 and Agrin in the acute injury groups. <b>Conclusions:</b> In conclusion, the synaptic regions displayed substantial adaptations within the first 48 h, with the presynaptic region recovering rapidly and the postsynaptic region recovering slowly. This relationship suggests that significant increases in Agrin and NRG1 play a crucial role in maintaining the integrity of these structures.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antenatal Sildenafil for Congenital Diaphragmatic Hernia: A Systematic Review and Bayesian Meta-Analysis of Preclinical Studies.","authors":"Tamara M Hundscheid, Ilaria Amodeo, Giacomo Cavallaro, Carlijn R Hooijmans, František Bartoš, Eduardo Villamor","doi":"10.3390/biomedicines13092274","DOIUrl":"10.3390/biomedicines13092274","url":null,"abstract":"<p><p><b>Background</b>: In congenital diaphragmatic hernia (CDH), pulmonary hypoplasia and pulmonary hypertension are major causes of morbidity and mortality. Antenatal treatment with sildenafil has shown some promising protective effects in experimental CDH, but no systematic review has yet evaluated the preclinical evidence on this topic. <b>Methods</b>: PubMed and EMBASE databases were searched for studies using antenatal sildenafil in animal models of CDH. Bayesian model-averaged (BMA) meta-analysis was used to calculate Bayes factors (BFs). The BF₁₀ is the ratio of the probability of the data under the alternative hypothesis (presence of effect) over the probability of the data under the null hypothesis (absence of effect). Risk of bias was assessed by the SYRCLE tool. <b>Results</b>: We included 18 studies (14 nitrofen and 4 surgical CDH). The BMA analysis showed inconclusive evidence (BF₁₀ between 0.33 and 3) for the presence of an effect of sildenafil in fetal survival (7 studies, BF₁₀ = 1.25) or in lung hypoplasia as assessed by the lung-to-body weight ratio (16 studies, BF₁₀ = 2.04). In contrast, the BMA analysis showed conclusive evidence (BF₁₀ > 3) in favor of a positive effect of sildenafil on small pulmonary arteries medial wall thickness (12 studies, BF₁₀ = 1499), radial alveolar count (6 studies, BF₁₀ = 167.57), interalveolar septa thickness (4 studies, BF₁₀ = 56.86), distal airway complexity (3 studies, BF₁₀ = 7.95), mean saccular airspace diameter (2 studies, BF₁₀ = 7.61), total lung capacity (2 studies, BF₁₀ = 6.91), lung compliance (2 studies, BF₁₀ = 5.19), and VEGF expression (5 studies, BF₁₀ = 10.62). <b>Conclusions</b>: In preclinical models of CDH, antenatal sildenafil rescues pulmonary vascular remodeling and airway/airspace morphometric alterations.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Platelet-Rich Fibrin in Combination with Synthetic Bone Grafting: A Systematic Review.","authors":"Rosana Costa, Alicia Carvalho, Paula López-Jarana, Vitória Costa, Marta Relvas, Filomena Salazar, Tomás Infante da Câmara, Miguel Nunes Vasques, Marco Infante da Câmara","doi":"10.3390/biomedicines13092266","DOIUrl":"10.3390/biomedicines13092266","url":null,"abstract":"<p><p><b>Background:</b> In atrophic posterior maxillary regions, sub-antral surgery is often used for rehabilitation with implants. In order to stimulate bone regeneration, autogenous, xenogenic, alloplastic and platelet-rich fibrin (PRF) grafts are commonly used. <b>Aim:</b> To assess the effectiveness of PRF alone or combination with synthetic bone substitutes on bone formation, implant stability, and survival in sub-antral surgery. <b>Materials and Methods:</b> A literature review was carried out from September 2024 to April 2025, according to PRISMA guidelines using the PubMed, Cochrane Library, Wiley, ScienceDirect, and Web of Science databases. From a total of 601 articles identified, 11 met the inclusion criteria and were selected for analysis. <b>Results:</b> PRF in combination with synthetic materials has shown potential benefits, especially in increasing biomechanical stability and bone formation. Although, most studies have not reported statistically significant differences when comparing the use of synthetic material alone against its combination with PRF. <b>Discussion:</b> The use of synthetic grafts in combination with PRF has become increasingly common in sub-antral implant procedures. PRF promotes angiogenesis, osteoprogenitor cell differentiation and bone regeneration, favouring the healing and remodelling process of the tissues, as well as greater stability and longevity of the implant. <b>Conclusions:</b> The combination of PRF with synthetic bone grafting shows promising results; however, further studies are needed to confirm the efficacy of PRF in maxillary sinus grafts in conjunction with the use of biomaterials.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-Hydrazone-Modified Calcium Phosphate Scaffolds: Fabrication, Characterization, and Drug Delivery Potential.","authors":"Teodora Jakovljević, Jelena Stanisavljević, Julijana Stevanović, Miloš Petković, Ivana Z Matić, Miloš Papić, Suzana Živanović, Tamara Matić, Vukašin Ugrinović, Djordje Janaćković, Biljana Ljujić, Djordje Veljović","doi":"10.3390/biomedicines13092270","DOIUrl":"10.3390/biomedicines13092270","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Recent advancements in biomaterials aimed at closely mimicking natural biological tissues hold great promise for hard tissue regeneration and controlled drug release due to their superior physical, chemical, and biological properties. This study aimed to develop multi-ion doped calcium hydroxyapatite (HAp) scaffolds with chitosan-based coatings for localized drug delivery, incorporating a novel hydrazone compound with potential anticancer activity. <b>Methods</b>: HAp powders doped with magnesium (Mg²⁺), strontium (Sr²⁺), and varying fluoride (F^-) contents (0-2 mol.%) were synthesized via a hydrothermal method. Scaffolds were fabricated using the sponge replica technique and subsequently coated with chitosan or a chitosan-hydrazone blend. Dopant incorporation was confirmed by electron dispersive X-ray spectroscopy (EDS). Phase composition and morphology were analyzed via X-ray diffraction (XRD) and scanning electron microscopy (SEM). Mechanical properties, bioactivity, cytotoxicity, and hydrazone release profiles were systematically evaluated. <b>Results</b>: EDS confirmed successful incorporation of Mg²⁺ and Sr²⁺ in all powders, while F^- was detected only in powders with 1 and 2 mol.% fluoride. XRD and SEM revealed the phase composition and scaffold microstructure. Chitosan coatings significantly improved scaffold compressive strength and reduced degradation rate, indicating enhanced stability in biological environments. The coated scaffolds supported MRC-5 fibroblast viability. The hydrazone compound exhibited dose-dependent antitumor cytotoxicity comparable to cisplatin and showed sustained release from scaffolds for up to 15 days. <b>Conclusions</b>: The combination of multi-ion doped HAp scaffolds and chitosan-hydrazone coatings provides a promising platform for bone tissue engineering and localized cancer therapy, demonstrating both mechanical stability and controlled, sustained drug release.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}