Biomedicines最新文献

{"title":"Skin Resilience and Biological Adaptability: Current Clinical Evidence on Micronutrients and Bioactive Compounds.","authors":"Daniela Mihalache, Alina-Mihaela Gurau, Gabriela Patrichi, Gabriela Gurau, Catalin-Bogdan Satala","doi":"10.3390/biomedicines14092111","DOIUrl":"10.3390/biomedicines14092111","url":null,"abstract":"<p><p>Skin resilience and biological adaptability describe the capacity of cutaneous tissue to preserve structural integrity and functional stability under intrinsic aging and environmental stress. Rather than introducing a novel biological pathway, this framework integrates established domains, including redox homeostasis, extracellular matrix maintenance, inflammatory regulation, and barrier function, into a measurable construct. Micronutrients and bioactive compounds participate in these processes; however, the strength and clinical relevance of current evidence require critical evaluation. This structured narrative review appraises mechanistic and clinical studies published between 2000 and 2025 investigating nutritional modulation of measurable skin parameters. Electronic database searches (PubMed/MEDLINE, Scopus, Web of Science) were conducted using predefined terms related to skin aging, resilience-related endpoints, and micronutrient interventions. Controlled human trials were prioritized, and mechanistic findings were distinguished from clinical outcomes. Current clinical evidence most consistently supports a role for carotenoids and antioxidant vitamins in enhancing cutaneous redox stability and short-term photoprotection. Collagen-based formulations and selected polyphenols demonstrate modest improvements in elasticity, hydration, and wrinkle-related parameters, though many trials are limited by small sample sizes, short duration, and heterogeneous endpoints. Overall, micronutrients appear to function primarily as modulators of adaptive capacity rather than as primary drivers of structural rejuvenation. Larger, longer-term randomized trials employing standardized resilience-related endpoints are needed to clarify the magnitude and durability of clinically meaningful effects.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149058141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broad-Coverage Lipidomics Reveals Temporal Lipid Remodeling and Candidate Serum Biomarkers After Acute Spinal Cord Injury in Rats.","authors":"Tengfei Zhao, Hanxu Huang, Chaoran Shi, Feng Zhang, Qionghua Wu, Fangcai Li, Kan Xu","doi":"10.3390/biomedicines14092107","DOIUrl":"10.3390/biomedicines14092107","url":null,"abstract":"<p><p><b>Objective:</b> To systematically characterize temporal lipid metabolic remodeling in serum and spinal cord tissue following spinal cord injury (SCI) and to identify candidate serum lipid biomarkers associated with disease progression. <b>Methods:</b> A moderate-to-severe SCI model was established in Sprague-Dawley rats using a modified MASCIS weight-drop contusion protocol. Serum and injured spinal cord tissue were collected at 6 h, 1 d, 3 d, 7 d, and 14 d after injury. Broad-coverage lipidomic profiling was performed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). In parallel, transcriptomic profiling of injured spinal cord tissue was conducted, followed by bioinformatic analyses and RT-qPCR validation of selected metabolic genes. <b>Results:</b> Broad-coverage lipidomic analysis showed significant and dynamic lipid remodeling in both serum and spinal cord tissue after SCI. The serum lipid profile exhibited a multiphasic pattern, with early suppression, intermediate activation, and late-stage remodeling. Structural phospholipid subclasses showed coordinated temporal changes after injury. Long-chain acylcarnitines (LCACs) increased rapidly in serum during the acute phase and then gradually accumulated in the injured spinal cord tissue. In contrast, cholesteryl esters (CEs) progressively accumulated in spinal cord tissue, while showing a biphasic pattern in serum, with an early decrease followed by a rebound increase. Receiver operating characteristic curve analysis showed that LCACs and CEs had excellent discriminatory performance across multiple post-injury stages. <b>Conclusions:</b> SCI is associated with extensive lipid metabolic reprogramming in both serum and spinal cord tissue. LCACs and CEs represent promising candidate serum biomarkers closely associated with disease progression. Multi-omics analyses further suggest that serum lipid remodeling parallels lipid metabolic and inflammatory reprogramming in the injured spinal cord, while the contribution of local pathology to circulating LCAC and CE changes remains to be clarified.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione-Related Responses to Hyperglycemic and Hyperosmotic Stress in Cultured Human Retinal Pericytes and Endothelial Cells: An Exploratory Study.","authors":"Ecaterina Pavlovschi, Felicia Darii, Valeriana Pantea, Ștefan Curtev, Cristina Trocin, Serghei Curlat, Dan-Cristian Ușurelu, Tatiana Mărițoi, Olga Tagadiuc","doi":"10.3390/biomedicines14092104","DOIUrl":"10.3390/biomedicines14092104","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Hyperglycemic and hyperosmotic stress may disturb glutathione-dependent antioxidant defenses in retinal vascular cells. This exploratory in vitro study evaluated glutathione-related biochemical endpoints in separate human retinal pericyte and endothelial-cell experiments and to identify candidate endpoints for independent validation. <b>Methods:</b> Passage-3 human retinal pericytes and endothelial cells were exposed for 24 h to five conditions: 5.5 mmol/L glucose (control), 25 or 50 mmol/L glucose, and 5.5 mmol/L glucose supplemented with 19.5 or 44.5 mmol/L mannitol. Glutathione peroxidase (GPx), glutathione reductase (GR), total glutathione, reduced glutathione (GSH), oxidized glutathione (GSSG), the GSH/GSSG ratio, glutathione S-transferase (GST), and gamma-glutamyl transferase (GGT) were measured in a mixture of culture medium and cell lysate. Each condition comprised 10 technical replicate wells from one culture experiment; the cell types were examined in separate, unmatched runs. <b>Results:</b> In the pericyte experiment, GPx and GR decreased across several glucose and mannitol conditions, and GGT increased by 39-72%. In the separate endothelial experiment, GGT was the dominant response (eta squared = 0.763), increasing by 66-116%; GGT at 50 mmol/L glucose was 30% higher than the corresponding 44.5 mmol/L mannitol condition. <b>Conclusions:</b> The study identifies GGT as the principal candidate endpoint for biological replication and GPx and GR as additional candidates in pericytes. It does not support comparisons between cell types. All findings are preliminary because they derive from technical wells in single, unmatched experiments.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Adjunct Therapies with Surgery for Gastroenteropancreatic Neuroendocrine Tumors: Indications, Timing, and Outcomes.","authors":"Kathryn Cavallo, Naris Nilubol","doi":"10.3390/biomedicines14092105","DOIUrl":"10.3390/biomedicines14092105","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are rare biologically heterogeneous neoplasms but with increasing incidence in recent years. Surgery remains the backbone of treatment; however, long-term recurrence is common even after curative resection. Despite expanding treatment options, the optimal perioperative integration of adjunct therapies in conjunction with surgery remains incompletely defined, as available evidence is largely retrospective and heterogeneous. This review critically synthesizes available evidence on the role of perioperative therapies in the surgical management of GEP-NENs, identifies key knowledge gaps, and highlights areas where multidisciplinary decision-making is required. <b>Methods:</b> A targeted narrative literature review was performed in PubMed/MEDLINE (2001-2026), supplemented by ClinicalTrials.gov and major society guidelines. Predefined search terms were used to include \"gastroenteropancreatic neuroendocrine tumor\", \"GEP-NET\", \"pancreatic neuroendocrine tumor\", \"surgery\", \"palliative surgery\", \"neoadjuvant\", \"adjuvant\", \"PRRT\", \"somatostatin analogue\", \"chemotherapy\", \"targeted therapy\", \"immune checkpoint inhibitor\", and \"liver-directed therapy\". Studies were selected based on relevance, with emphasis on comprehensive reviews, key clinical studies, and major guideline statements. This approach was chosen due to varied literature and scarce high-quality prospective evidence. <b>Results:</b> Neoadjuvant PRRT and CAPTEM-based chemotherapy demonstrate encouraging activity in selected patients with SSTR-positive or borderline resectable pNETs, though evidence is predominantly retrospective and R0 resection rates are inconsistent across modalities. Adjuvant systemic therapy lacks high-quality evidence of benefit in well-differentiated GEP-NETs whereas platinum-based chemotherapy following resection of NEC represents the only setting where adjuvant treatment is commonly recommended, albeit without randomized trial support. Liver-directed therapies, including thermal ablation, TACE, and TARE, play complementary roles in selected patients with hepatic metastases. The overall evidence base is largely retrospective and heterogenous. Management should be individualized through multidisciplinary discussion.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging Autoimmunity and Hyperinflammation: Shared Immunological Pathways Among Autoimmune/Inflammatory Syndrome Induced by Adjuvants, Systemic Lupus Erythematosus, and Macrophage Activation Syndrome.","authors":"Gabriela D'El Rei Tamaki, Guilherme de Oliveira Vitiello, Érika Bevilaqua Rangel","doi":"10.3390/biomedicines14092101","DOIUrl":"10.3390/biomedicines14092101","url":null,"abstract":"<p><p>Autoimmune/inflammatory syndrome induced by adjuvants (ASIA) is characterized by immune dysregulation associated with exposure to exogenous substances with immunostimulatory properties, particularly in susceptible individuals. Systemic lupus erythematosus (SLE) is a complex and heterogeneous autoimmune disease characterized by loss of immune tolerance, autoantibody production, and immune complex deposition. Macrophage activation syndrome (MAS) is a life-threatening hyperinflammatory syndrome characterized by excessive activation of macrophages and T lymphocytes and may occur as a severe complication of SLE and other inflammatory conditions. This review explores the clinical and immunological overlap among ASIA, SLE, and MAS, with particular attention to the potential contribution of adjuvant-related exposures to immune activation in susceptible individuals. Shared and convergent immunological pathways, including activation of innate and adaptive immunity, loss of immune tolerance, macrophage activation, and dysregulation of cytokine networks involving IL-1β, IL-6, IL-17, IL-18, TNF-α, and interferon-γ, are discussed. Rather than representing an established causal or sequential pathway, the relationship among these conditions is considered within a conceptual framework based on overlapping immunological mechanisms. Clinically, overlapping manifestations among ASIA, SLE, and MAS may pose diagnostic challenges and contribute to underrecognition or delayed treatment. Careful assessment of potential triggering exposures, integration of clinical and laboratory findings, and prompt recognition of hyperinflammatory manifestations are important for appropriate clinical management. This review highlights the need for further mechanistic and prospective studies to determine the clinical relevance of the immunological overlap among ASIA, SLE, and MAS, validate potential biomarkers, and evaluate targeted therapeutic strategies directed at shared inflammatory pathways.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spike Protein at the Crossroads of Long-COVID and Vaccine-Induced Immune Thrombotic Thrombocytopenia Syndromes: A Cardio-Hematological Perspective.","authors":"Elifcan Aladag, Ugur Nadir Karakulak, Ibrahim Celalettin Haznedaroglu","doi":"10.3390/biomedicines14092090","DOIUrl":"10.3390/biomedicines14092090","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein is central to viral infectivity, host-pathogen interactions, and immune recognition. Beyond its indispensable role in viral entry, accumulating experimental and clinical evidence indicates that spike protein may exert pleiotropic biological effects involving endothelial, cardiovascular, hematological, neurological, and immunological pathways. During natural infection, these effects occur in the context of active viral replication, additional viral antigens, and systemic inflammation, whereas vaccination induces a fundamentally different, transient exposure to a prefusion-stabilized spike antigen without viral propagation. In this review, we critically examine the molecular and clinicopathological properties of the SARS-CoV-2 spike protein and the biological differences between infection-derived and vaccine-derived spike exposure. Particular emphasis is placed on endothelial dysfunction, platelet and complement activation, immune-thrombosis, persistent viral antigens and tissue reservoirs in Long-COVID, and the distinct anti-PF4-mediated pathophysiology of vaccine-induced immune thrombotic thrombocytopenia (VITT). Emerging evidence suggests that persistent or dysregulated spike-related antigen exposure may contribute to chronic multisystem manifestations in a subset of individuals following SARS-CoV-2 infection; however, evidence linking persistent vaccine-derived spike to chronic clinical syndromes remains substantially more limited and does not currently establish causality. Integrating these observations, we propose the 'Long-Spike' hypothesis as a hypothesis-generating conceptual framework rather than a defined clinical syndrome. Further prospective studies integrating ultrasensitive antigen detection, tissue-based analyses, immunophenotyping, and cardiovascular and hematological biomarkers are required to determine the clinical relevance and causal significance of persistent spike-related antigens.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149058129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Proteome-Wide Mendelian Randomization and Multi-Omics Analysis Identify ADM and CFH as Candidate Genes for Osteoarthritis.","authors":"Haoyang Li, Dongliang Gong, Jun Yang, Zixiang Wang, Junlei Lv, Changan Guo","doi":"10.3390/biomedicines14092096","DOIUrl":"10.3390/biomedicines14092096","url":null,"abstract":"<p><p><b>Background</b>: Osteoarthritis (OA) is a prevalent degenerative joint disease lacking effective disease-modifying therapies, which necessitates the discovery of key genes for mechanistic exploration and therapeutic development. <b>Methods</b>: We integrated three large-scale cis-protein quantitative trait locus datasets and two OA genome-wide association study summary statistics to screen candidate proteins by two-stage proteome-wide Mendelian randomization (MR). Causal association reliability was validated via summary-data-based Mendelian randomization (SMR) and Bayesian colocalization analyses. A phenome-wide association study (PheWAS) was performed to evaluate potential pleiotropic effects of the candidates. Subsequently, transcriptomic and single-cell RNA sequencing datasets were employed to evaluate the candidate genes' expression stability, classification efficacy in the in vitro models of OA, cell-specific enrichment, and pseudotime expression dynamics in cartilage. Finally, drug repurposing potential was explored by integrating drug-gene interaction database searches and molecular docking. <b>Results</b>: Two-stage cis-pQTL MR combined with cis-eQTL-based SMR analysis identified 14 plasma proteins with consistent effects at the protein and transcript levels. RNA-seq revealed that <i>adrenomedullin (ADM)</i> and <i>complement factor H (CFH)</i> were upregulated in two in vitro models of OA, and both genes exhibited favorable classification efficacy in these models. Bayesian colocalization analysis provided evidence of shared causal variants for <i>ADM</i>, and PheWAS did not detect significant pleiotropic associations for <i>ADM</i> or <i>CFH</i> across the tested phenotypes. Single-cell analysis indicated that <i>ADM</i> was enriched in pre-fibrocartilage chondrocytes with biphasic pseudotime expression, whereas <i>CFH</i> was widely expressed across chondrocyte subsets. Database screening identified 15 potential drugs for <i>ADM</i> and 6 for <i>CFH</i>. <b>Conclusions</b>: Combining MR, multi-omics and pharmacological evidence, we prioritized <i>ADM</i> and <i>CFH</i> as OA candidate genes.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abdominal Obesity, Testosterone Deficiency and Sedentary Lifestyle in Young Men from the General Population: The Relationship with Semen Quality, Hormonal and Metabolic Status.","authors":"Ludmila Osadchuk, Alexander Osadchuk","doi":"10.3390/biomedicines14092097","DOIUrl":"10.3390/biomedicines14092097","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Background:&lt;/b&gt; The demographic crisis observed in industrialized countries has been accompanied by a decline in the reproductive potential of human populations. One of the causes for the global deterioration in men's reproductive health is adiposity and sedentary lifestyle, leading to reduced fertility and a decrease in androgen status. The aim of the study was to assess the association between abdominal obesity and semen parameters, including sperm DNA fragmentation, as well as key indicators of hormonal and metabolic status in young men from the general population. &lt;b&gt;Methods:&lt;/b&gt; Our cross-sectional study was conducted among Russian and Belarusian men living in six cities. The examination of men (n = 1283, median age 23 years) included questionnaires, anthropometry, and the collection of peripheral blood and semen samples. Semen analysis was conducted according to WHO guidelines. Sperm DNA fragmentation index (DFI) was evaluated using the SCSA method. The serum and seminal zinc concentration was determined using spectrophotometry and direct calorimetry. Hormonal and metabolic levels were measured using commercial kits. Waist circumference (WC) was used as a surrogate marker of abdominal adiposity. Participants were allocated to three phenotypes: non-obese (control, WC &lt; 94 cm), excess abdominal fat (94 ≤ WC &lt; 102 cm) and abdominal obesity (WC ≥ 102 cm). &lt;b&gt;Results:&lt;/b&gt; In our study population, 85.8% of participants were young (under 30 years of age), 8.6% had excess abdominal fat, 5.7% had abdominal obesity (AO), and 65.2% had normozoospermia. Men with AO were characterized by a decrease in semen volume, total sperm count, sperm concentration and progressive motility compared to the non-obese control. DFI was significantly higher in obese compared to non-obese men. Obese participants had significantly lower testosterone and inhibin B levels, but higher FSH and leptin levels compared to non-obese men. Men with AO had elevated serum zinc levels but reduced seminal zinc content. Abdominal obesity was also associated with significant metabolic changes, in particular, with increased levels of triglycerides, total cholesterol, low-density lipoproteins, fasting glucose and uric acid. AO-related testosterone deficiency (serum testosterone level ≤ 12.1 nmol/L) was detected in 38.7% of obese men and was accompanied by elevated DFI, levels of FSH, and zinc, as well as unfavorable changes in metabolic indicators. Physically active AO men (who engaged in recreational sports or physical labor) demonstrated reduced anthropometric indicators of AO and leptin levels, increased LH and testosterone levels, and improved carbohydrate and lipid metabolism compared to sedentary AO men. &lt;b&gt;Conclusions:&lt;/b&gt; AO is accompanied by a decrease in the activity of Leydig and Sertoli cells, as well as an imbalance of the hypothalamic-pituitary-testicular axis and metabolic status. AO, associated with testosterone deficiency or a sedentary lifestyle, is a potential r","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Efficacy and Surgical Outcomes of Cervical Foley Catheter Tourniquet in Placenta Accreta Spectrum Disorders.","authors":"Huseyin Durukan, Hamza Yildiz, Kasim Akay, Ezgi Oktay, Faik Gurkan Yazici","doi":"10.3390/biomedicines14092095","DOIUrl":"10.3390/biomedicines14092095","url":null,"abstract":"<p><p><b>Objective:</b> We aimed to evaluate the impact of the cervical Foley catheter tourniquet on perioperative surgical interventions, transfusion requirements, and maternal-neonatal outcomes in patients with placenta accreta spectrum (PAS). <b>Materials and Methods:</b> In this single-center retrospective cohort study, 265 patients with confirmed PAS were categorized into a tourniquet group (<i>n</i> = 87, cervical Foley catheter tourniquet applied after fetal delivery) and a control group (<i>n</i> = 178, standard hemostasis) following an institutional protocol update. Perioperative surgical interventions, hematologic parameters, transfusion needs, and maternal-neonatal outcomes were compared. <b>Results:</b> Baseline characteristics were generally comparable between groups. The decrease in hemoglobin (ΔHb) was significantly smaller in the tourniquet group than in controls (1.51 ± 1.11 vs. 1.75 ± 1.57 g/dL; <i>p</i> = 0.045). The tourniquet group had significantly fewer maternal ICU admissions (4.6% vs. 12.4%; <i>p</i> = 0.049); however, ICU length of stay among admitted patients did not differ significantly between groups (median 1.0 vs. 1.0 days; <i>p</i> = 0.585). The peripartum hysterectomy rate was lower in the tourniquet group (8.0% vs. 16.3%), although this difference did not reach conventional statistical significance (<i>p</i> = 0.085). Operative time was longer in the tourniquet group (105.0 vs. 60.0 min; <i>p</i> < 0.001). No maternal deaths occurred in either cohort. <b>Conclusions:</b> The cervical Foley catheter tourniquet is a feasible and low-cost surgical adjunct that was associated with a smaller perioperative decline in hemoglobin and lower maternal ICU admission rates, alongside higher rates of uterine-preserving procedures. However, because actual blood loss was not directly quantified and transfusion frequency remained high, its true independent effect on intraoperative blood loss and total transfusion requirements remains uncertain.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-Derived Transcriptomics Reveals Distinct IFNγ-Associated Myeloid States and Mitochondrial Complex I Programs in Rheumatoid Arthritis and Osteoarthritis.","authors":"Heekyong R Bae, Byeongchan Son, Seong-Su Choi, Hye-Bin Jeong, Howard A Young, Eun-Young Kwon","doi":"10.3390/biomedicines14092094","DOIUrl":"10.3390/biomedicines14092094","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Macrophages are key contributors to the pathogenesis of rheumatoid arthritis (RA) and osteoarthritis (OA), but their disease-associated immunometabolic states remain incompletely understood. <b>Methods:</b> Here, we comparatively analyzed patient-derived transcriptomic data from synovial tissues and synovial macrophages in RA and OA. <b>Results:</b> RA synovial tissues showed stronger interferon-γ (IFNγ)-associated signatures and reduced mitochondrial Complex I-related programs compared with OA. Similar reductions in oxidative phosphorylation, mitochondrial translation, and Complex I-related transcription were observed in synovial fluid-derived RA macrophages relative to control macrophages. In an independent dataset of synovial tissue-derived OA macrophages, inflammatory-like OA macrophages exhibited concurrent enrichment of glycolytic and oxidative metabolic programs. Bulk RNA-seq analysis of synovial monocytes within a common study framework further showed that reduced mitochondrial translation and Complex I-related programs were preferentially associated with leukocyte-rich inflammatory RA. At single-cell resolution, IFN-responsive monocytes enriched in leukocyte-rich RA showed reduced transcriptional representation of both nuclear- and mtDNA-encoded Complex I components. <b>Conclusions:</b> Collectively, these findings link an IFN-responsive inflammatory myeloid state in RA with coordinated suppression of mitochondrial translation and Complex I-related transcription, suggesting a transcriptional state that may increase susceptibility to mitochondrial Complex I dysfunction during persistent inflammation.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"14 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149058038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}