Biomedical Journal最新文献

Communicating across distances - biological functions of extracellular vesicles. 跨越距离的交流--细胞外囊泡的生物功能。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-30 DOI: 10.1016/j.bj.2024.100792

Aila Akosua Kattner

引用次数: 0

The Ratio of Remaining to Expected Small Bowel Length Predicts Enteral Autonomy in Pediatric Patients with Short Bowel Syndrome. 剩余小肠长度与预期小肠长度之比可预测小儿短肠综合征患者的肠内自主性。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-19 DOI: 10.1016/j.bj.2024.100791

Chia-Wei Chang, Pai-Jui Yeh, Hung-Hsian Lai, Mi-Chi Chen, Yung-Ching Ming, Jing-Yao Lai, Ming-Wei Lai

{"title":"The Ratio of Remaining to Expected Small Bowel Length Predicts Enteral Autonomy in Pediatric Patients with Short Bowel Syndrome.","authors":"Chia-Wei Chang, Pai-Jui Yeh, Hung-Hsian Lai, Mi-Chi Chen, Yung-Ching Ming, Jing-Yao Lai, Ming-Wei Lai","doi":"10.1016/j.bj.2024.100791","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100791","url":null,"abstract":"Background: Pediatric patients with short bowel syndrome (SBS) often require long-term parenteral nutrition and intravenous fluid support (PN) until enteral autonomy (EA). However, long-term PN accounts for many complications. We aimed to investigate the outcome and predictors of EA in these patients.Material and methods: This retrospective observational study was conducted in Children's Medical Center, Chang Gung Memorial Hospital, a tertiary hospital in Northern Taiwan. Twenty-four patients afflicted with short bowel syndrome between 2002 and 2021 were included. Demographics, operation results, follow-up status, complications, and outcomes were reviewed.Results: Among the 24 patients, 14 were males (58%). The median age at bowel resection was 3 days (IQR, 1.3 to 28.8 days). The most common etiologies were total/subtotal intestinal aganglionosis (TIA) (N=6) and malrotation with midgut volvulus (N=6). The median length of the residual small intestine was 25cm (IQR, 7.8 to 71.3cm). Ten (41.7%) had preserved ileocecal valve, and 14 (58.3%) had colon-in-continuity. Intestinal failure-associated liver disease (IFALD) occurred in 14 patients (58.3%), but none had advanced disease. Seven (29.2%) patients achieved enteral autonomy after 10.1±7.3 months. Five patients (21%) expired due to sepsis. Logistic regression and Kaplan-Meier analysis showed the predictors of enteral autonomy were remaining-to-expected small bowel length ratio > 25% and the absence of IFALD.Conclusions: In this pediatric short bowel syndrome study, enteral autonomy was achieved in 29% after a mean PN duration of 10 months. The remaining-to-expected small bowel length ratio at bowel resection was the most critical predictor of enteral autonomy.","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudogene: Relevant or Irrelevant? 伪基因：相关还是不相关？

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-19 DOI: 10.1016/j.bj.2024.100790

Yang-Hsiang Lin, Chau-Ting Yeh, Cheng-Yi Chen, Kwang-Huei Lin

引用次数: 0

Cytidine Deaminase Enhances Liver Cancer Invasion by Modulating Epithelial-Mesenchymal Transition via NFκB Signaling. 胞苷脱氨酶通过 NFκB 信号调节上皮-间质转化，从而增强肝癌的侵袭能力

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-19 DOI: 10.1016/j.bj.2024.100789

Chia-Jung Liao, Yang-Hsiang Lin, Huei-Tzu Chien, Yi-Wen Wang, Tzu-Kang Lin, Chau-Ting Yeh, Kwang-Huei Lin

{"title":"Cytidine Deaminase Enhances Liver Cancer Invasion by Modulating Epithelial-Mesenchymal Transition via NFκB Signaling.","authors":"Chia-Jung Liao, Yang-Hsiang Lin, Huei-Tzu Chien, Yi-Wen Wang, Tzu-Kang Lin, Chau-Ting Yeh, Kwang-Huei Lin","doi":"10.1016/j.bj.2024.100789","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100789","url":null,"abstract":"Background: Cancer metastasis is the leading cause of cancer-related deaths, underscoring the importance of understanding its underlying mechanisms. Hepatocellular carcinoma (HCC), a highly malignant type of cancer, was selected as our research model.Material and methods: We aimed to develop high-metastatic cell lines using in vitro and in vivo selection strategies and identify critical metastasis-related genes through microarray analyses by comparing them with parental cells.Results: Our results showed that the high-metastatic cell lines exhibited significantly stronger invasion abilities than parental cells. Microarray analyses identified cytidine deaminase (CDA), a gene associated with systemic chemotherapy resistance, as one of the overexpressed genes in the high-metastatic cells. Data analysis from The Cancer Genome Atlas Program revealed that while CDA is downregulated in HCC, patients with high CDA expression tend to have poorer prognoses. Cell models confirmed that CDA overexpression enhances cell migration and invasion, whereas CDA knockdown inhibits these abilities. Investigating the key molecules involved in the epithelial-mesenchymal transition (EMT), we found that CDA overexpression increases the expression of fascin, N-cadherin, β-catenin, and snail while decreasing E-cadherin expression. Conversely, CDA knockdown produced opposite results. Additionally, we discovered that CDA regulates NF-κB signaling, which controls the expression of N-cadherin, thereby promoting the invasion capability of HCC cells.Conclusions: We isolated highly metastatic cells and identified potential HCC metastasis-related genes. CDA promotes cell invasion by regulating EMT through the NF-κB pathway. Future studies are warranted to explore the potential of CDA as a biomarker for prognosis and therapeutic decision-making.","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological functions of extracellular vesicles from mammalian cells 哺乳动物细胞外囊泡的生物功能

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-12 DOI: 10.1016/j.bj.2024.100788

引用次数: 0

Effects of recombinant SARS-CoV-2 spike protein variants on red blood cells parameters and red blood cell distribution width. 重组 SARS-CoV-2 穗状病毒蛋白变体对红细胞参数和红细胞分布宽度的影响

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-07 DOI: 10.1016/j.bj.2024.100787

Francesco Dima, Gian Luca Salvagno, Giuseppe Lippi

引用次数: 0

Advancements in Chondrocyte 3-Dimensional Embedded Culture: Implications for Tissue Engineering and Regenerative Medicine. 软骨细胞三维嵌入培养的进展：组织工程和再生医学的意义》。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-09-03 DOI: 10.1016/j.bj.2024.100786

Yu-Ying Chu, Atsuhiko Hikita, Yukiyo Asawa, Kazuto Hoshi

{"title":"Advancements in Chondrocyte 3-Dimensional Embedded Culture: Implications for Tissue Engineering and Regenerative Medicine.","authors":"Yu-Ying Chu, Atsuhiko Hikita, Yukiyo Asawa, Kazuto Hoshi","doi":"10.1016/j.bj.2024.100786","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100786","url":null,"abstract":"Cartilage repair necessitates regenerative medicine because of the unreliable healing mechanism of cartilage. To yield a sufficient number of cells for transplantation, chondrocytes must be expanded in culture. However, in 2D culture, chondrocytes tend to lose their distinctive phenotypes and functionalities after serial passage, thereby limiting their efficacy for tissue engineering purposes. The mechanism of dedifferentiation in 2D culture can be attributed to various factors, including abnormal nuclear strength, stress-induced mitochondrial impairment, chromatin remodeling, ERK-1/2 and the p38/mitogen-activated protein kinase (MAPK) signaling pathway. These mechanisms collectively contribute to the loss of chondrocyte phenotype and reduced production of cartilage-specific extracellular matrix (ECM) components. Chondrocyte 3D culture methods have emerged as promising solutions to prevent dedifferentiation. Techniques, such as scaffold-based culture and scaffold-free approaches, provide chondrocytes with a more physiologically relevant environment, promoting their differentiation and matrix synthesis. These methods have been used in cartilage tissue engineering to create engineered cartilage constructs for transplantation and joint repair. However, chondrocyte 3D culture still has limitations, such as low viability and proliferation rate, and also difficulties in passage under 3D condition. These indicate challenges of obtaining a sufficient number of chondrocytes for large-scale tissue production. To address these issues, ongoing studies of many research groups have been focusing on refining culture conditions, optimizing scaffold materials, and exploring novel cell sources such as stem cells to enhance the quality and quantity of engineered cartilage tissues. Although obstacles remain, continuous endeavors to enhance culture techniques and overcome limitations offer a promising outlook for the advancement of more efficient strategies for cartilage regeneration.","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles in fetal-maternal immune tolerance "胎儿和母体免疫耐受中的细胞外囊泡

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-29 DOI: 10.1016/j.bj.2024.100785

引用次数: 0

miRNA-mediated regulation of clock gene expression in men and women with colorectal cancer and possible consequences for disease management. miRNA 介导的男性和女性结直肠癌患者时钟基因表达调控及对疾病管理的可能影响。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-10 DOI: 10.1016/j.bj.2024.100784

Iveta Herichová

{"title":"miRNA-mediated regulation of clock gene expression in men and women with colorectal cancer and possible consequences for disease management.","authors":"Iveta Herichová","doi":"10.1016/j.bj.2024.100784","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100784","url":null,"abstract":"Background: The incidence and mortality of colorectal cancer (CRC) are persistently higher in men than in women. CRC malignancy is strongly influenced by small non-coding RNAs (miRNAs). Moreover, deregulation of the circadian molecular oscillator has been associated with CRC facilitation. To analyse possible cumulative effects of the above-mentioned factors on CRC progression, we focused on functions of sex-biased miRNAs associated with the clock genes per2 and/or cry2, which are involved in the cell cycle control and DNA damage response.Major findings: We identified miR-24, miR-92a, miR-181a, and miR-21 associated with per2 that are up-regulated in transformed colon tissue of men. miR-93, miR-17, miR-20a, and miR-24 with higher expression in males compared to females were linked to cry2. All these miRNAs possess oncogenic potential and exert their effects mainly via inhibition of the tumour suppressors phosphatase and tensin homolog (PTEN) and/or p53. Down-regulation of PTEN and p53 in men was further strengthened by inhibition of tumour suppressor per2. Oncogenic up-regulated miRNAs associated with per2 or cry2 in the transformed colon tissue of women were not detected.Conclusion: We conclude that the cancer-promoting, sex-biased miRNAs miR-24, miR-92a, miR-181a, miR-93, miR-17, miR-20a, and miR-21 associated with clock genes per2 and/or cry2 can contribute to the sex-dependent development of CRC via inhibition of the PTEN and p53 pathways.","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Prognosis of Patients with Myocardial Infarction after Light Therapy: A Preliminary Study. 光疗后心肌梗死患者的预后：初步研究。

IF 4.1 3区医学

Biomedical Journal Pub Date : 2024-08-07 DOI: 10.1016/j.bj.2024.100783

Wei-Chih Chin, Yu-Shu Huang, Lung-Sheng Wu, Kuang-Tso Lee, Chien-Te Ho, Chen Lin, Wei-Sheng Yang, I-Hang Chung, Pao-Hsien Chu

{"title":"The Prognosis of Patients with Myocardial Infarction after Light Therapy: A Preliminary Study.","authors":"Wei-Chih Chin, Yu-Shu Huang, Lung-Sheng Wu, Kuang-Tso Lee, Chien-Te Ho, Chen Lin, Wei-Sheng Yang, I-Hang Chung, Pao-Hsien Chu","doi":"10.1016/j.bj.2024.100783","DOIUrl":"https://doi.org/10.1016/j.bj.2024.100783","url":null,"abstract":"Background: Patients with myocardial infarction (MI) can have disturbed sleep, but little is known about the efficacy of light therapy on sleep and prognosis of patients with MI. We conducted a randomized controlled study to investigate its efficacy.Material and methods: This preliminary study included 34 patients with MI. They were randomized into the blue light and the white light groups during their stay in intensive care unit. 17 age and gender matched healthy controls were also enrolled. Actigraphy was used to evaluate objective sleep since enrollment. Delirium scales were used to screen delirium. Lab work-up including vitamin D level was performed at the baseline and discharge. We used Mann-Whitney U test or Wilcoxon signed-rank test to compare the difference between the MI group and the healthy control group, and the group difference after receiving light therapy.Results: Patients with MI had significantly lower vitamin D level than healthy controls (p<0.001). They also had significantly poorer sleep, as indicated by actigraphy parameters including sleep onset latency (p=0.01), sleep efficiency (p=0.002), wake after sleep onset (p<0.001) and awake times (p=0.002). No significant group difference was found by actigraphy after light therapy except a non-significant higher relative amplitude of the blue light group (p=0.061). Besides, vitamin D level of the blue light group increased significantly (p1=0.047, p2=0.045).Conclusions: Patients with MI had poorer sleep, highlighting the needs to develop interventions. Significantly increased vitamin D level and a non-significant better rest-active rhythm after light therapy suggest its potential with sleep and prognosis which warrants further investigation.","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0