Bioarchitecture最新文献_第6页

0.1 kilopascal difference for mechanophenotyping: soft matrix precisely regulates cellular architecture for invasion. 0.1千帕斯卡的机械表型差异:软基质精确调节细胞结构的入侵。

Bioarchitecture Pub Date : 2014-01-01 Epub Date: 2014-05-21 DOI: 10.4161/bioa.29175

Zhizhan Gu

{"title":"0.1 kilopascal difference for mechanophenotyping: soft matrix precisely regulates cellular architecture for invasion.","authors":"Zhizhan Gu","doi":"10.4161/bioa.29175","DOIUrl":"https://doi.org/10.4161/bioa.29175","url":null,"abstract":"Current knowledge understands the mesenchymal cell invasion in a 3D matrix as a combined process of cell-to-matrix adhesion based cell migration and matrix remodeling. Excluding cell invasion stimulated by cytokines and chemokines, the basal cell invasion itself is a complicated process that can be regulated by matrix ligand type, density, geometry, and stiffness, etc. Understanding such a complicated biological process requires delicate dissections into simplified model studies by altering only one or two elements at a time. Past cell motility studies focusing on matrix stiffness have revealed that a stiffer matrix promotes 2D X-Y axis lateral cell motility. Here, we comment on two recent studies that report, instead of stiffer matrix, a softer matrix promotes matrix proteolysis and the formation of invadosome-like protrusions (ILPs) along the 3D Z axis. These studies also reveal that soft matrix precisely regulates such ILPs formation in the stiffness scale range of 0.1 kilopascal in normal cells. In contrast, malignant cells such as cancer cells can form ILPs in response to a much wider range of matrix stiffness. Further, different cancer cells respond to their own favorable range of matrix stiffness to spontaneously form ILPs. Thus, we hereby propose the idea of utilizing the matrix stiffness to precisely regulate ILP formation as a mechanophenotyping tool for cancer metastasis prediction and pathological diagnosis. ","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"4 3","pages":"116-8"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bioa.29175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32508449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Plant pathogenic bacteria target the actin microfilament network involved in the trafficking of disease defense components. 植物致病菌以参与疾病防御成分运输的肌动蛋白微丝网络为目标。

Bioarchitecture Pub Date : 2014-01-01 DOI: 10.4161/19490992.2014.980662

Joanna Jelenska, Yongsung Kang, Jean T Greenberg

引用次数: 16

Axonal trafficking of NMNAT2 and its roles in axon growth and survival in vivo. NMNAT2的轴突转运及其在轴突生长和存活中的作用。

Bioarchitecture Pub Date : 2013-09-01 Epub Date: 2013-11-07 DOI: 10.4161/bioa.27049

Stefan Milde, Jonathan Gilley, Michael P Coleman

{"title":"Axonal trafficking of NMNAT2 and its roles in axon growth and survival in vivo.","authors":"Stefan Milde, Jonathan Gilley, Michael P Coleman","doi":"10.4161/bioa.27049","DOIUrl":"https://doi.org/10.4161/bioa.27049","url":null,"abstract":"The NAD-synthesizing enzyme NMNAT2 is critical for axon survival in primary culture and its depletion may contribute to axon degeneration in a variety of neurodegenerative disorders. Here we discuss several recent reports from our laboratory that establish a critical role for NMNAT2 in axon growth in vivo in mice and shed light on the delivery and turnover of this survival factor in axons. In the absence of NMNAT2, axons fail to extend more than a short distance beyond the cell body during embryonic development, implying a requirement for NMNAT2 in axon maintenance even during development. Furthermore, we highlight findings regarding the bidirectional trafficking of NMNAT2 in axons on a vesicle population that undergoes fast axonal transport in primary culture neurites and in mouse sciatic nerve axons in vivo. Surprisingly, loss of vesicle association boosts the axon protective capacity of NMNAT2, an effect that is at least partially mediated by a longer protein half-life of cytosolic NMNAT2 variants. Analysis of wild-type and variant NMNAT2 in mouse sciatic nerves and Drosophila olfactory receptor neuron axons supports the existence of a similar mechanism in vivo, highlighting the potential for regulation of NMNAT2 stability and turnover as a mechanism to modulate axon degeneration in vivo. ","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"3 5","pages":"133-40"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bioa.27049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31910221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Hook1, microtubules, and Rab22: mediators of selective sorting of clathrin-independent endocytic cargo proteins on endosomes. Hook1、微管和Rab22:核内体上不依赖网格蛋白的内吞转运蛋白选择性分选的介质。

Bioarchitecture Pub Date : 2013-09-01 DOI: 10.4161/bioa.26638

Lymarie Maldonado-Báez, Julie G Donaldson

{"title":"Hook1, microtubules, and Rab22: mediators of selective sorting of clathrin-independent endocytic cargo proteins on endosomes.","authors":"Lymarie Maldonado-Báez, Julie G Donaldson","doi":"10.4161/bioa.26638","DOIUrl":"https://doi.org/10.4161/bioa.26638","url":null,"abstract":"Clathrin-independent endocytosis (CIE) mediates the internalization of many plasma membrane (PM) proteins involved in homeostasis, immune response, and signaling. CIE cargo molecules are internalized independent of clathrin, and dynamin, and modulated by the small G protein Arf6. After internalization the CIE cargo proteins either follow a default pathway of trafficking to lysosomes for degradation or follow a pathway where they are routed directly to the recycling endosomes for return to the PM. The selective endosomal sorting of molecules like CD44, CD98, and CD147, which are involved in cell-cell and cell-extracellular interactions, indicates that sorting mechanisms dictate the post-endocytic fate of CIE cargo proteins. In a recent study, we identified sorting signals that specify the endosomal trafficking of CIE cargo proteins and uncover a role for Hook1 as an endosomal cargo adaptor that routes CIE cargo to the recycling endosomes. Furthermore, we found that Hook1, microtubules, and Rab22a work in coordination to directly recycle the cargo and facilitate cell spreading. Here, we discuss our current view on the endosomal sorting of CIE cargo proteins and their molecular regulators. ","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"3 5","pages":"141-6"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bioa.26638","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31910603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Human fronto-parietal and parieto-hippocampal pathways represent behavioral priorities in multiple spatial reference frames. 人类额-顶叶和顶叶-海马通路在多个空间参考框架中代表行为优先级。

Bioarchitecture Pub Date : 2013-09-01 Epub Date: 2013-12-09 DOI: 10.4161/bioa.27462

Sara M Szczepanski, Yuri B Saalmann

引用次数: 7

The heavy chain has its day: regulation of myosin-II assembly. 重链有它的一天:肌球蛋白ii组装的调节。

Bioarchitecture Pub Date : 2013-07-01 DOI: 10.4161/bioa.26133

Natalya G Dulyaninova, Anne R Bresnick

引用次数: 78

Coiled coil cytoskeletons collaborate in polar growth of Streptomyces. 螺旋状细胞骨架在链霉菌的极性生长中协同作用。

Bioarchitecture Pub Date : 2013-07-01 DOI: 10.4161/bioa.26194

Nora Ausmees

{"title":"Coiled coil cytoskeletons collaborate in polar growth of Streptomyces.","authors":"Nora Ausmees","doi":"10.4161/bioa.26194","DOIUrl":"https://doi.org/10.4161/bioa.26194","url":null,"abstract":"Streptomyces is a multicellular mycelial bacterium, which exhibits pronounced cell polarity and grows by extension of the hyphal tips. Similarly to other polarly growing walled cells, such as filamentous fungi or pollen tubes, Streptomyces hyphae face an intrinsic problem: addition of new cell wall material causes structural weakness of the elongating tip. Cellular strategies employed by walled cells to cope with this problem are not well understood. We have identified a coiled coil protein FilP, with properties similar to those of animal intermediate filament (IF) proteins, which somehow confers rigidity and elasticity to the Streptomyces hyphae. In a recent publication we showed that FilP forms extensive cis-interconnected networks, which likely explain its biological function in determining the mechanical properties of the cells. Surprisingly, the intrinsically non-dynamic cytoskeletal network of FilP exhibits a dynamic behavior in vivo and assembles into growth-dependent polar gradients. We show that apical accumulation of FilP is dependent on its interaction with the main component of the Streptomyces polarisome, DivIVA. Thus, the same polarisome complex that orchestrates cell elongation, also recruits an additional stress-bearing structure to the growing tips with an intrinsically weak cell wall. Similar strategy might be used by all polarly growing walled cells.","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"3 4","pages":"110-2"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bioa.26194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31706145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

In vivo evidence for mTORC2-mediated actin cytoskeleton rearrangement in neurons. 神经元中mtorc2介导的肌动蛋白细胞骨架重排的体内证据。

Bioarchitecture Pub Date : 2013-07-01 DOI: 10.4161/bioa.26497

Nico Angliker, Markus A Rüegg

引用次数: 53

MicroRNA-23b regulates cellular architecture and impairs motogenic and invasive phenotypes during cancer progression. 在癌症进展过程中，MicroRNA-23b调节细胞结构并损害运动性和侵袭性表型。

Bioarchitecture Pub Date : 2013-07-01 DOI: 10.4161/bioa.26134

Loredana Pellegrino, Jonathan Krell, Laura Roca-Alonso, Justin Stebbing, Leandro Castellano

引用次数: 0

The cytoskeleton and neurite initiation. 细胞骨架和神经突起始。

Bioarchitecture Pub Date : 2013-07-01 DOI: 10.4161/bioa.26259

Kevin C Flynn

引用次数: 107