Bioarchitecture最新文献

Bioarchitecture Pub Date : 2016-11-01 DOI: 10.1080/19490992.2016.1291050

Peter Gunning, G V Shivashankar

引用次数: 0

The impact of tropomyosins on actin filament assembly is isoform specific. 原肌凝蛋白对肌动蛋白丝组装的影响是同种异构体特异性的。

Bioarchitecture Pub Date : 2016-07-03 Epub Date: 2016-07-15 DOI: 10.1080/19490992.2016.1201619

Miro Janco, Teresa T Bonello, Alex Byun, Adelle C F Coster, Helene Lebhar, Irina Dedova, Peter W Gunning, Till Böcking

{"title":"The impact of tropomyosins on actin filament assembly is isoform specific.","authors":"Miro Janco, Teresa T Bonello, Alex Byun, Adelle C F Coster, Helene Lebhar, Irina Dedova, Peter W Gunning, Till Böcking","doi":"10.1080/19490992.2016.1201619","DOIUrl":"https://doi.org/10.1080/19490992.2016.1201619","url":null,"abstract":"Tropomyosin (Tpm) is an α helical coiled-coil dimer that forms a co-polymer along the actin filament. Tpm is involved in the regulation of actin's interaction with binding proteins as well as stabilization of the actin filament and its assembly kinetics. Recent studies show that multiple Tpm isoforms also define the functional properties of distinct actin filament populations within a cell. Subtle structural variations within well conserved Tpm isoforms are the key to their functional specificity. Therefore, we purified and characterized a comprehensive set of 8 Tpm isoforms (Tpm1.1, Tpm1.12, Tpm1.6, Tpm1.7, Tpm1.8, Tpm2.1, Tpm3.1, and Tpm4.2), using well-established actin co-sedimentation and pyrene fluorescence polymerization assays. We observed that the apparent affinity (Kd(app)) to filamentous actin varied in all Tpm isoforms between ∼0.1-5 μM with similar values for both, skeletal and cytoskeletal actin filaments. The data did not indicate any correlation between affinity and size of Tpm molecules, however high molecular weight (HMW) isoforms Tpm1.1, Tpm1.6, Tpm1.7 and Tpm2.1, showed ∼3-fold higher cooperativity compared to low molecular weight (LMW) isoforms Tpm1.12, Tpm1.8, Tpm3.1, and Tpm4.2. The rate of actin filament elongation in the presence of Tpm2.1 increased, while all other isoforms decreased the elongation rate by 27-85 %. Our study shows that the biochemical properties of Tpm isoforms are finely tuned and depend on sequence variations in alternatively spliced regions of Tpm molecules. ","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"6 4","pages":"61-75"},"PeriodicalIF":0.0,"publicationDate":"2016-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19490992.2016.1201619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34561931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Geometric control and modeling of genome reprogramming. 基因组重编程的几何控制和建模。

Bioarchitecture Pub Date : 2016-07-03 Epub Date: 2016-07-19 DOI: 10.1080/19490992.2016.1201620

Caroline Uhler, G V Shivashankar

引用次数: 15

Post-polymerization crosstalk between the actin cytoskeleton and microtubule network. 肌动蛋白细胞骨架和微管网络之间的聚合后串扰。

Bioarchitecture Pub Date : 2016-05-03 DOI: 10.1080/19490992.2016.1171428

E Emily Joo, Kenneth M Yamada

引用次数: 12

Where are the limits of the centrosome? 中心体的界限在哪里?

Bioarchitecture Pub Date : 2016-05-03 DOI: 10.1080/19490992.2016.1168957

Irina B Alieva, Rustem E Uzbekov

{"title":"Where are the limits of the centrosome?","authors":"Irina B Alieva, Rustem E Uzbekov","doi":"10.1080/19490992.2016.1168957","DOIUrl":"https://doi.org/10.1080/19490992.2016.1168957","url":null,"abstract":"The centrosome is a key component of the cell is involved in the processes of cell division, cell motility, intracellular transport, organization of the microtubules (MT) network and the production of cilia and flagella. The peculiarity of this organelle is that its boundaries are not clearly defined, the centrioles at the center of the centrosome are surrounded by electron dense pericentriolar material, the size and protein composition of this centrosome component experiences significant transformation during the cell cycle. It has been shown in this study that within the centrosome different proteins occupy different areas corresponding to: MT nucleation region (defined as gamma-tubulin-stained area), regulatory region (defined as kinase pEg2-stained area) and motor proteins region (kinesin-like motor XlEg5-stained area). The boundary of pEg2 is near 1.3 times greater while XlEg5 is 3.0 times greater than that of gamma-tubulin. Thus, the size of the centrosome, determined according to the structural electron microscopy (EM) analysis (about 1 µm) corresponds to the regulatory proteins area, but the actual functional centrosome size defined at the motor proteins region, is more than twice the size. ","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"6 3","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"2016-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19490992.2016.1168957","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34415478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Ankyrin-B in lens architecture and biomechanics: Just not tethering but more 镜头结构和生物力学中的锚蛋白b:不仅仅是系住，而是更多

Bioarchitecture Pub Date : 2016-03-03 DOI: 10.1080/19490992.2016.1156284

P. Rao, R. Maddala

引用次数: 6

Spatiotemporal phosphoregulation of Lgl: Finding meaning in multiple on/off buttons Lgl的时空磷酸化调控:在多个开/关按钮中找到意义

Bioarchitecture Pub Date : 2016-02-26 DOI: 10.1080/19490992.2016.1149290

S. Moreira, Eurico Morais-de-Sá

引用次数: 6

Using both strands: The fundamental nature of antisense transcription 使用两条链:反义转录的基本性质

Bioarchitecture Pub Date : 2016-01-02 DOI: 10.1080/19490992.2015.1130779

S. Murray, J. Mellor

引用次数: 16

SNAREs in the maturation and function of LROs LROs成熟和功能中的SNAREs

Bioarchitecture Pub Date : 2016-01-02 DOI: 10.1080/19490992.2015.1131890

R. Jani, S. Mahanty, Subba Rao Gangi Setty

引用次数: 5

Mechanoprotection by skeletal muscle caveolae 骨骼肌小泡的机械保护作用

Bioarchitecture Pub Date : 2016-01-02 DOI: 10.1080/19490992.2015.1131891

H. Lo, T. Hall, R. Parton

{"title":"Mechanoprotection by skeletal muscle caveolae","authors":"H. Lo, T. Hall, R. Parton","doi":"10.1080/19490992.2015.1131891","DOIUrl":"https://doi.org/10.1080/19490992.2015.1131891","url":null,"abstract":"abstract Caveolae, small bulb-like pits, are the most abundant surface feature of many vertebrate cell types. The relationship of the structure of caveolae to their function has been a subject of considerable scientific interest in view of the association of caveolar dysfunction with human disease. In a recent study Lo et al.1 investigated the organization and function of caveolae in skeletal muscle. Using quantitative 3D electron microscopy caveolae were shown to be predominantly organized into multilobed structures which provide a large reservoir of surface-connected membrane underlying the sarcolemma. These structures were preferentially disassembled in response to changes in membrane tension. Perturbation or loss of caveolae in mouse and zebrafish models suggested that caveolae can protect the muscle sarcolemma against damage in response to excessive membrane activity. Flattening of caveolae to release membrane into the bulk plasma membrane in response to increased membrane tension can allow cell shape changes and prevent membrane rupture. In addition, disassembly of caveolae can have widespread effects on lipid-based plasma membrane organization. These findings suggest that the ability of the caveolar membrane system to respond to mechanical forces is a crucial evolutionarily-conserved process which is compromised in disease conditions associated with mutations in key caveolar components.","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"6 1","pages":"22 - 27"},"PeriodicalIF":0.0,"publicationDate":"2016-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85307542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23