Bioarchitecture最新文献_第2页

Emergence of Form from Function - Mechanical Engineering Approaches to Probe the Role of Stem Cell Mechanoadaptation in Sealing Cell Fate. 从功能中产生形态——用机械工程方法探讨干细胞机械适应在封闭细胞命运中的作用。

Bioarchitecture Pub Date : 2016-01-01 Epub Date: 2016-10-14 DOI: 10.1080/19490992.2016.1229729

Melissa L Knothe Tate, Peter W Gunning, Vittorio Sansalone

{"title":"Emergence of Form from Function - Mechanical Engineering Approaches to Probe the Role of Stem Cell Mechanoadaptation in Sealing Cell Fate.","authors":"Melissa L Knothe Tate, Peter W Gunning, Vittorio Sansalone","doi":"10.1080/19490992.2016.1229729","DOIUrl":"https://doi.org/10.1080/19490992.2016.1229729","url":null,"abstract":"Stem cell \"mechanomics\" refers to the effect of mechanical cues on stem cell and matrix biology, where cell shape and fate are intrinsic manifestations of form and function. Before specialization, the stem cell itself serves as a sensor and actuator; its structure emerges from its local mechanical milieu as the cell adapts over time. Coupling of novel spatiotemporal imaging and computational methods allows for linking of the energy of adaptation to the structure, biology and mechanical function of the cell. Cutting edge imaging methods enable probing of mechanisms by which stem cells' emergent anisotropic architecture and fate commitment occurs. A novel cell-scale model provides a mechanistic framework to describe stem cell growth and remodeling through mechanical feedback; making use of a generalized virtual power principle, the model accounts for the rate of doing work or the rate of using energy to effect the work. This coupled approach provides a basis to elucidate mechanisms underlying the stem cell's innate capacity to adapt to mechanical stimuli as well as the role of mechanoadaptation in lineage commitment. An understanding of stem cell mechanoadaptation is key to deciphering lineage commitment, during prenatal development, postnatal wound healing, and engineering of tissues.","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"6 5","pages":"85-103"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19490992.2016.1229729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39975353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Leukocyte adhesion and polarization: Role of glycosylphosphatidylinositol-anchored proteins 白细胞粘附和极化:糖基磷脂酰肌醇锚定蛋白的作用

Bioarchitecture Pub Date : 2015-12-02 DOI: 10.1080/19490992.2015.1127466

D. D. Richardson, M. Fernandez-Borja

{"title":"Leukocyte adhesion and polarization: Role of glycosylphosphatidylinositol-anchored proteins","authors":"D. D. Richardson, M. Fernandez-Borja","doi":"10.1080/19490992.2015.1127466","DOIUrl":"https://doi.org/10.1080/19490992.2015.1127466","url":null,"abstract":"ABSTRACT Leukocyte traffic out of the blood stream is crucial for an adequate immune response. Leukocyte extravasation is critically dependent on the binding of leukocyte integrins to their endothelial counterreceptors. This interaction enables the firm adhesion of leukocytes to the luminal side of the vascular wall and allows for leukocyte polarization, crawling and diapedesis. Leukocyte adhesion, polarization and migration requires the orchestrated regulation of integrin adhesion/de-adhesion dynamics and actin cytoskeleton rearrangements. Adhesion strength depends on conformational changes of integrin molecules (affinity) as well as the number of integrin molecules engaged at adhesion sites (valency). These two processes can be independently regulated and several molecules modulate either one or both processes. Cholesterol-rich membrane domains (lipid rafts) participate in integrin regulation and play an important role in leukocyte adhesion, polarization and motility. In particular, lipid raft-resident glycosyl-phosphatidyl-inositol-anchored proteins (GPI-APs) have been reported to regulate leukocyte adhesion, polarization and motility in both integrin-dependent and independent manners. Here, we present our recent discovery concerning the novel role of the GPI-AP prion protein (PrP) in the regulation of β1 integrin-mediated monocyte adhesion, migration and shape polarization in the context of existing literature on GPI-AP-dependent regulation of integrins.","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"2 1","pages":"61 - 69"},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76720839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Possible regulation of caveolar endocytosis and flattening by phosphorylation of F-BAR domain protein PACSIN2/Syndapin II F-BAR结构域蛋白PACSIN2/Syndapin II的磷酸化可能调控空泡内吞和扁平

Bioarchitecture Pub Date : 2015-12-02 DOI: 10.1080/19490992.2015.1128604

Y. Senju, S. Suetsugu

{"title":"Possible regulation of caveolar endocytosis and flattening by phosphorylation of F-BAR domain protein PACSIN2/Syndapin II","authors":"Y. Senju, S. Suetsugu","doi":"10.1080/19490992.2015.1128604","DOIUrl":"https://doi.org/10.1080/19490992.2015.1128604","url":null,"abstract":"ABSTRACT. Caveolae are flask-shaped invaginations of the plasma membrane. The BAR domain proteins form crescent-shaped dimers, and their oligomeric filaments are considered to form spirals at the necks of invaginations, such as clathrin-coated pits and caveolae. PACSIN2/Syndapin II is one of the BAR domain-containing proteins, and is localized at the necks of caveolae. PACSIN2 is thought to function in the scission and stabilization of caveolae, through binding to dynamin-2 and EHD2, respectively. These two functions are considered to be switched by PACSIN2 phosphorylation by protein kinase C (PKC) upon hypotonic stress and sheer stress. The phosphorylation decreases the membrane binding affinity of PACSIN2, leading to its removal from caveolae. The removal of the putative oligomeric spiral of PACSIN2 from caveolar membrane invaginations could lead to the deformation of caveolae. Indeed, PACSIN2 removal from caveolae is accompanied by the recruitment of dynamin-2, suggesting that the removal provides space for the function of dynamin-2. Otherwise, the removal of PACSIN2 decreases the stability of caveolae, which could result in the flattening of caveolae. In contrast, an increase in the amount of EHD2 restored caveolar stability. Therefore, PACSIN2 at caveolae stabilizes caveolae, but its removal by phosphorylation could induce both caveolar endocytosis and flattening.","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"149 1","pages":"70 - 77"},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82838170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Composite biopolymer scaffolds shape muscle nucleus: Insights and perspectives from Drosophila 复合生物聚合物支架塑造肌核:来自果蝇的见解和观点

Bioarchitecture Pub Date : 2015-10-03 DOI: 10.1080/19490992.2015.1106061

Shuoshuo Wang, T. Volk

{"title":"Composite biopolymer scaffolds shape muscle nucleus: Insights and perspectives from Drosophila","authors":"Shuoshuo Wang, T. Volk","doi":"10.1080/19490992.2015.1106061","DOIUrl":"https://doi.org/10.1080/19490992.2015.1106061","url":null,"abstract":"Contractile muscle fibers produce enormous intrinsic forces during contraction/relaxation waves. These forces are directly applied to their cytoplasmic organelles including mitochondria, sarcoplasmic reticulum, and multiple nuclei. Data from our analysis of Drosophila larval somatic muscle fibers suggest that an intricate network of organized microtubules (MT) intermingled with Spectrin-Repeat-Containing Proteins (SRCPs) are major structural elements that protect muscle organelles and maintain their structure and position during muscle contraction. Whereas the perinuclear MT network provides structural rigidity to the myonucleus, the SRCPs Nesprin and Spectraplakin form semiflexible filamentous biopolymer networks, providing nuclei with the elasticity required to resist the contractile cytoplasmic forces produced by the muscle. Spectrin repeats are domains found in numerous structural proteins, which are able to unfold under tension and are subject to mechanical stresses in the cell. This unique composite scaffold combines rigidity and resilience in order to neutralize the oscillating cellular forces occurring during muscle contraction/relaxation waves and thereby protect myonuclei. We suggest that the elastic properties of SRCPs are critical for nuclear protection and proper function in muscle fibers.","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"44 1","pages":"35 - 43"},"PeriodicalIF":0.0,"publicationDate":"2015-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74348771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Intracellular transport and cell surface delivery of the neural cell adhesion molecule (NCAM) 神经细胞粘附分子(NCAM)的细胞内转运和细胞表面递送

Bioarchitecture Pub Date : 2015-10-03 DOI: 10.1080/19490992.2015.1118194

Iryna Leshchyns’ka, V. Sytnyk

引用次数: 6

High-content analysis of Rab protein function at the ER-Golgi interface 兔er -高尔基界面蛋白功能的高含量分析

Bioarchitecture Pub Date : 2015-10-03 DOI: 10.1080/19490992.2015.1102826

George Galea, J. Simpson

引用次数: 17

Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo 斑马鱼胚胎中植物定位的背系和生殖系决定因素的皮质深度和差异运输

Bioarchitecture Pub Date : 2015-08-03 DOI: 10.1080/19490992.2015.1080891

E. Welch, F. Pelegri

引用次数: 14

How the SAC gets the axe: Integrating kinetochore microtubule attachments with spindle assembly checkpoint signaling. SAC如何得到斧头:整合着丝点微管附着物与纺锤体组装检查点信号。

Bioarchitecture Pub Date : 2015-01-01 Epub Date: 2015-10-02 DOI: 10.1080/19490992.2015.1090669

Shivangi Agarwal, Dileep Varma

引用次数: 6

Cytoskeletal self-organization in neuromorphogenesis. 神经形态发生中的细胞骨架自组织。

Bioarchitecture Pub Date : 2014-03-01 Epub Date: 2014-05-21 DOI: 10.4161/bioa.29070

Leif Dehmelt

引用次数: 4

Filamin A and Big2: a shared endocytic pathway. 丝蛋白A和Big2:一条共享的内吞途径。

Bioarchitecture Pub Date : 2014-03-01 Epub Date: 2014-04-07 DOI: 10.4161/bioa.28516

Volney L Sheen

{"title":"Filamin A and Big2: a shared endocytic pathway.","authors":"Volney L Sheen","doi":"10.4161/bioa.28516","DOIUrl":"https://doi.org/10.4161/bioa.28516","url":null,"abstract":"Neural proliferation, migration and differentiation require reorganization of the actin cytoskeleton and regulation of vesicle trafficking to provide stability in maintaining cell adhesions, allow for changes in cell shape, and establishing cell polarity. Human disorders involving the actin-binding Filamin A (FLNA) and vesicle trafficking Brefeldin-associated guanine exchange factor 2 (BIG2 is encoded by the ARFGEF2 gene) proteins are implicated in these various developmental processes, resulting in a malformation of cortical development called periventricular heterotopia (nodules along the ventricular lining) and microcephaly (small brain). Here we discuss several recent reports from our laboratory that demonstrate a shared role for both proteins in actin-associated vesicle trafficking, which is required to maintain the expression and stability of cell adhesion and cell cycle associated molecules during cortical development. While changes in FLNA and BIG2 have first been linked to disorders involving the central nervous system, increasing reports suggest they are associated with aberrant development of various other organ systems in the body. These studies suggest that vesicle trafficking defects in FLN-GEF dependent pathways may contribute to a much broader phenotype than previously realized. ","PeriodicalId":89329,"journal":{"name":"Bioarchitecture","volume":"4 2","pages":"53-7"},"PeriodicalIF":0.0,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bioa.28516","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32242457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10