Bioengineered最新文献_第7页

Establishment and characterization of a highly metastatic hepatocellular carcinoma cell line. 高转移性肝癌细胞系的建立和特征描述。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-01-07 DOI: 10.1080/21655979.2023.2296775

Jiacheng Huang, Mengqing Sun, Menglan Wang, Anning Yu, Huilin Zheng, Chiwen Bu, Jie Zhou, Yu Zhang, Yiting Qiao, Zhenhua Hu

{"title":"Establishment and characterization of a highly metastatic hepatocellular carcinoma cell line.","authors":"Jiacheng Huang, Mengqing Sun, Menglan Wang, Anning Yu, Huilin Zheng, Chiwen Bu, Jie Zhou, Yu Zhang, Yiting Qiao, Zhenhua Hu","doi":"10.1080/21655979.2023.2296775","DOIUrl":"10.1080/21655979.2023.2296775","url":null,"abstract":"The prevalence of alcohol-related hepatocellular carcinoma (HCC) has been increasing during the last decade. Cancer research requires cell lines suitable for both in vitro and in vivo assays. However, there is a lack of cell lines with a high in vivo metastatic capacity for this HCC subtype. Herein, a new HCC cell line was established, named HCC-ZJ, using cells from a patient diagnosed with alcohol-related HCC. The karyotype of HCC-ZJ was 46, XY, del (p11.2). Whole-exome sequencing identified several genetic variations in HCC-Z that occur frequently in alcohol-associated HCC, such as mutations in TERT, CTNNB1, ARID1A, CDKN2A, SMARCA2, and HGF. Cell counting kit-8 assays, colony formation assays, and Transwell assays were performed to evaluate the proliferation, migration, and sensitivity to sorafenib and lenvatinib of HCC-Z in vitro. HCC-ZJ showed a robust proliferation rate, a weak foci-forming ability, a strong migration capacity, and a moderate invasion tendency in vitro. Finally, the tumorigenicity and metastatic capacity of HCC-Z were evaluated using a subcutaneous xenograft model, an orthotopic xenograft model, and a tail-veil injection model. HCCZJ exhibited strong tumorigenicity in the subcutaneous xenograft and orthotopic tumor models. Moreover, HCC-ZJ spontaneously formed pulmonary metastases in the orthotopic tumor model. In summary, a new HCC cell line derived from a patient with alcohol-related HCC was established, which showed a high metastatic capacity and could be applied for in vitro and in vivo experiments during pre-clinical research.Highlights• An alcohol-related HCC cell line, HCC-ZJ, was established• HCC-ZJ was applicable for in vitro functional experiment and gene editing• HCC-ZJ was applicable for in vivo tumor growth and spontaneous metastasis models.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"15 1","pages":"2296775"},"PeriodicalIF":4.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statement of Retraction: Long noncoding RNA UBA6-AS1 inhibits the malignancy of ovarian cancer cells via suppressing the decay of UBA6 mRNA. 撤回声明：长非编码 RNA UBA6-AS1 通过抑制 UBA6 mRNA 的衰变抑制卵巢癌细胞的恶性程度。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI: 10.1080/21655979.2024.2299622

引用次数: 0

Large intestinal nutritional and physicochemical parameters from different dog sizes reshape canine microbiota structure and functions in vitro. 不同体型犬的大肠营养和理化参数在体外重塑了犬微生物群的结构和功能。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-03-12 DOI: 10.1080/21655979.2024.2325713

Charlotte Deschamps, Delphine Humbert, Sandrine Chalancon, Caroline Achard, Emmanuelle Apper, Sylvain Denis, Stéphanie Blanquet-Diot

{"title":"Large intestinal nutritional and physicochemical parameters from different dog sizes reshape canine microbiota structure and functions in vitro.","authors":"Charlotte Deschamps, Delphine Humbert, Sandrine Chalancon, Caroline Achard, Emmanuelle Apper, Sylvain Denis, Stéphanie Blanquet-Diot","doi":"10.1080/21655979.2024.2325713","DOIUrl":"10.1080/21655979.2024.2325713","url":null,"abstract":"Different dog sizes are associated with variations in large intestinal physiology including gut microbiota, which plays a key role in animal health. This study aims to evaluate, using the CANIM-ARCOL (Canine Mucosal Artificial Colon), the relative importance of gut microbes versus physicochemical and nutritional parameters of the canine colonic environment in shaping microbiota structure and functions. CANIM-ARCOL was set up to reproduce nutrient availability, bile acid profiles, colonic pH, and transit time from small, medium, or large dogs according to in vivo data, while bioreactors were all inoculated with a fecal sample collected from medium size dogs (n = 2). Applying different dog size parameters resulted in a positive association between size and gas or SCFA production, as well as distinct microbiota profiles as revealed by 16S Metabarcoding. Comparisons with in vivo data from canine stools and previous in vitro results obtained when CANIM-ARCOL was inoculated with fecal samples from three dog sizes revealed that environmental colonic parameters were sufficient to drive microbiota functions. However, size-related fecal microbes were necessary to accurately reproduce in vitro the colonic ecosystem of small, medium, and large dogs. For the first time, this study provides mechanistic insights on which parameters from colonic ecosystem mainly drive canine microbiota in relation to dog size. The CANIM-ARCOL can be used as a relevant in vitro platform to unravel interactions between food or pharma compounds and canine colonic microbiota, under different dog size conditions. The potential of the model will be extended soon to diseased situations (e.g. chronic enteropathies or obesity).","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"15 1","pages":"2325713"},"PeriodicalIF":4.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensor in smart food traceability system for food safety and security. 用于食品安全和安保的智能食品溯源系统中的生物传感器。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-02-01 DOI: 10.1080/21655979.2024.2310908

Catarina Meliana, Jingjing Liu, Pau Loke Show, Sze Shin Low

{"title":"Biosensor in smart food traceability system for food safety and security.","authors":"Catarina Meliana, Jingjing Liu, Pau Loke Show, Sze Shin Low","doi":"10.1080/21655979.2024.2310908","DOIUrl":"10.1080/21655979.2024.2310908","url":null,"abstract":"The burden of food contamination and food wastage has significantly contributed to the increased prevalence of foodborne disease and food insecurity all over the world. Due to this, there is an urgent need to develop a smarter food traceability system. Recent advancements in biosensors that are easy-to-use, rapid yet selective, sensitive, and cost-effective have shown great promise to meet the critical demand for onsite and immediate diagnosis and treatment of food safety and quality control (i.e. point-of-care technology). This review article focuses on the recent development of different biosensors for food safety and quality monitoring. In general, the application of biosensors in agriculture (i.e. pre-harvest stage) for early detection and routine control of plant infections or stress is discussed. Afterward, a more detailed advancement of biosensors in the past five years within the food supply chain (i.e. post-harvest stage) to detect different types of food contaminants and smart food packaging is highlighted. A section that discusses perspectives for the development of biosensors in the future is also mentioned.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"15 1","pages":"2310908"},"PeriodicalIF":4.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10841032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retracted article: Functional analysis of ceRNA network of lncRNA TSIX/miR-34a-5p/RBP2 in acute myocardial infarction based on GEO database. 被撤回的文章：基于GEO数据库的急性心肌梗死中lncRNA TSIX/miR-34a-5p/RBP2的ceRNA网络功能分析

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2021-11-16 DOI: 10.1080/21655979.2021.2006865

Jiezhong Lin, Jianyi Zhou, Guiting Xie, Xiongwei Xie, Yanfang Luo, Jinguang Liu

引用次数: 0

Unlocking the biodegradative potential of native white-rot fungi: a comparative study of fiberbank organic pollutant mycoremediation. 挖掘本地白腐真菌的生物降解潜力：纤维库有机污染物菌核修复比较研究。

IF 4.2 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-09-02 DOI: 10.1080/21655979.2024.2396642

Burcu Hacıoğlu, Gabriel Dupaul, Gabriela Paladino, Mattias Edman, Erik Hedenström

{"title":"Unlocking the biodegradative potential of native white-rot fungi: a comparative study of fiberbank organic pollutant mycoremediation.","authors":"Burcu Hacıoğlu, Gabriel Dupaul, Gabriela Paladino, Mattias Edman, Erik Hedenström","doi":"10.1080/21655979.2024.2396642","DOIUrl":"https://doi.org/10.1080/21655979.2024.2396642","url":null,"abstract":"Fiberbanks refer to a type of fibrous sediment originated by the forestry and wood pulping industry in Sweden. These anthropogenic sediments are significantly contaminated with potentially toxic elements, and a diverse array of organic pollutants. Additionally, these sediments are of environmental concern due to their potential role in greenhouse gas emissions. Given the environmental risks posed by these sediments, the development of effective remediation strategies is of critical importance. However, no specialized methods have been established yet for the cleanup of this specific type of contaminated sediments. To identify effective fungal species for the mycoremediation of the fiberbank substrate, we performed a detailed screening experiment. In this research, we primarily aimed at assessing both the growth capacity and the proficiency in degrading organic pollutants of 26 native white-rot fungi (WRF) species. These species were sourced from natural forest environments in northern Sweden. The experimental setup involved evaluating the WRF on plates containing fiberbank material with a central Hagem-agar disc to closely monitor the interaction of these species with fiberbank substrates. Among the fungi tested, Laetiporus sulphureus exhibited the highest growth area percentage at 72%, followed by Hymenochaete tabacina at 68% and Diplomitoporus crustulinus at 67%. For the removal of 2-3 ring polycyclic aromatic hydrocarbons (PAHs), Phellinus punctatus led with 68%, with Cystostereum muraii at 57% and Diplomitoporus crustulinus at 49%. Regarding the removal percentage of 4-6 ring PAHs, Diplomitoporus crustulinus showed the highest efficiency at 44%, followed by Phlebia tremellosa at 40% and Phlebiopsis gigantea at 28%.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"15 1","pages":"2396642"},"PeriodicalIF":4.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statement of Retraction: The urinary exosomes derived from premature infants attenuate cisplatin-induced acute kidney injury in mice via microRNA-30a-5p/ mitogen-activated protein kinase 8 (MAPK8). 撤回声明：早产儿尿液外泌体通过microRNA-30a-5p/丝裂原活化蛋白激酶8（MAPK8）减轻顺铂诱导的小鼠急性肾损伤。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI: 10.1080/21655979.2024.2299611

引用次数: 0

Statement of Retraction: Propofol inhibits migration and induces apoptosis of pancreatic cancer PANC-1 cells through miR-34a-mediated E-cadherin and LOC285194 signals. 撤回声明：丙泊酚通过 miR-34a 介导的 E-cadherin 和 LOC285194 信号抑制胰腺癌 PANC-1 细胞的迁移并诱导其凋亡。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-05-30 DOI: 10.1080/21655979.2024.2360264

引用次数: 0

Statement of Retraction: Bone marrow mesenchymal stem cells combined with Atractylodes macrocephala polysaccharide attenuate ulcerative colitis. 撤回声明：骨髓间充质干细胞与白术多糖结合可减轻溃疡性结肠炎。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI: 10.1080/21655979.2024.2299627

引用次数: 0

Statement of Retraction: Inhibition of Long non-coding RNA zinc finger antisense 1 improves functional recovery and angiogenesis after focal cerebral ischemia via microRNA-144-5p/fibroblast growth factor 7 axis. 撤回声明：抑制长非编码RNA锌指反义1可通过microRNA-144-5p/成纤维细胞生长因子7轴改善局灶性脑缺血后的功能恢复和血管生成。

IF 4.9 4区生物学

Bioengineered Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI: 10.1080/21655979.2024.2299583

引用次数: 0