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Systematic engineering of Escherichia coli for biosynthesis of 3-hydroxypropionic acid from glucose and malonate. 以葡萄糖和丙二酸酯为原料合成3-羟基丙酸的大肠杆菌系统工程研究。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-24 DOI: 10.1186/s12896-026-01140-2
Yingying Wang, Meihui Cao, Mei Hu, Hengrui Xu, Yafei Du, Changxing Sun, Lingfei Kong, Yu Luo, Xiaoyu Liu, Jianming Yang, Bo Liang
{"title":"Systematic engineering of Escherichia coli for biosynthesis of 3-hydroxypropionic acid from glucose and malonate.","authors":"Yingying Wang, Meihui Cao, Mei Hu, Hengrui Xu, Yafei Du, Changxing Sun, Lingfei Kong, Yu Luo, Xiaoyu Liu, Jianming Yang, Bo Liang","doi":"10.1186/s12896-026-01140-2","DOIUrl":"10.1186/s12896-026-01140-2","url":null,"abstract":"<p><strong>Background: </strong>3-Hydroxypropionic acid (3-HP) is a promising C3 platform chemical with wide industrial applications. However, its microbial production remains limited by insufficient intracellular malonyl-CoA availability and metabolic imbalance.</p><p><strong>Result: </strong>In this study, we systematically engineered Escherichia coli for enhanced 3-HP biosynthesis. The malonate assimilation genes (matB, smatPQM) and 3-HP biosynthesis gene (mcr) were chromosomally integrated using CRISPR/Cas9, resulting in a plasmid-free, antibiotic-free strain (WYY04) that produced 21.97 mM 3-HP, 0.51-fold higher than the plasmid-based system. Further improvement was achieved by CRISPRi-mediated repression of fatty acid biosynthesis genes (fabD, fabF), increasing 3-HP titer by 66%. Introduction of a malonyl-CoA-responsive FapR/fapO biosensor enabled dynamic regulation of mcr expression, enhancing 3-HP production by 59%. Through all these above engineering, the 3-HP production of the strain WYY19 increased by 2.29 times compared to that of the plasmid-expressing system. Under optimized fermentation conditions, the final engineered strain WYY19 produced 42.22 g/L 3-HP with the specific productivity of 0.69 g/g and 0.46 g/L/h from glucose and malonate in fed-batch bioreactor.</p><p><strong>Conclusions: </strong>This study demonstrates a robust, genetically stable, and scalable microbial platform for 3-HP biosynthesis.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anticancer activity of an apigenin-functionalized silica-coated silver oxide nanocomposite against human gastric adenocarcinoma cells (AGS): in vitro and in silico analysis. 芹菜素功能化二氧化硅包覆氧化银纳米复合材料对人胃腺癌细胞（AGS）的抗肿瘤活性：体外和硅分析。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-24 DOI: 10.1186/s12896-026-01118-0
Seyed Milad Mousavi Eshkelani, Reyhaneh Kouchakinejad, Sara Dabirian, Parastoo Vakilinezami, Kamyab Shokrani, Hamed Tajmehri, Hossein Zahmatkesh, Mahdi Shahriarinour, Behnam Rasti, Mohammad Nikpassand, Najmeh Ranji
{"title":"Anticancer activity of an apigenin-functionalized silica-coated silver oxide nanocomposite against human gastric adenocarcinoma cells (AGS): in vitro and in silico analysis.","authors":"Seyed Milad Mousavi Eshkelani, Reyhaneh Kouchakinejad, Sara Dabirian, Parastoo Vakilinezami, Kamyab Shokrani, Hamed Tajmehri, Hossein Zahmatkesh, Mahdi Shahriarinour, Behnam Rasti, Mohammad Nikpassand, Najmeh Ranji","doi":"10.1186/s12896-026-01118-0","DOIUrl":"10.1186/s12896-026-01118-0","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phyto mediated synthesis of copper oxide nanoparticles with Zingiber officinale: comprehensive bioactivity assessment against bacterial pathogens, cancer, and viruses. 植物介导的氧化铜纳米颗粒的合成：对细菌病原体、癌症和病毒的综合生物活性评估。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-21 DOI: 10.1186/s12896-026-01124-2
Mohamed K Y Soliman, Mai M El-Ashmony, Hager A Bendary, Fatma Rasslan, Salem S Salem
{"title":"Phyto mediated synthesis of copper oxide nanoparticles with Zingiber officinale: comprehensive bioactivity assessment against bacterial pathogens, cancer, and viruses.","authors":"Mohamed K Y Soliman, Mai M El-Ashmony, Hager A Bendary, Fatma Rasslan, Salem S Salem","doi":"10.1186/s12896-026-01124-2","DOIUrl":"10.1186/s12896-026-01124-2","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interpretable machine learning reveals metabolomic signatures: biomarkers and mechanisms in acute vs chronic angle-closure glaucoma. 可解释的机器学习揭示代谢组学特征：急性与慢性闭角型青光眼的生物标志物和机制。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-20 DOI: 10.1186/s12896-026-01137-x
Jun Ren, Zhuqing Li, Jianing Wu, Yingzhu Li, Yichao Qiu, Wenjun Cao, Xueli Chen, Shengjie Li
{"title":"Interpretable machine learning reveals metabolomic signatures: biomarkers and mechanisms in acute vs chronic angle-closure glaucoma.","authors":"Jun Ren, Zhuqing Li, Jianing Wu, Yingzhu Li, Yichao Qiu, Wenjun Cao, Xueli Chen, Shengjie Li","doi":"10.1186/s12896-026-01137-x","DOIUrl":"10.1186/s12896-026-01137-x","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147490578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Periodontal ligament stem cells-loaded photocrosslinking GelMA/PEGDA scaffolds for periodontal bone regeneration. 牙周韧带干细胞负载光交联GelMA/PEGDA支架用于牙周骨再生。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-19 DOI: 10.1186/s12896-026-01127-z
Bei Gao, Jie Yao, Yijie Wang, Chen Chen, Zhen Chai, Wanting Wan, Shiyang Wu, Zhongbo Liu, Rui Zou
{"title":"Periodontal ligament stem cells-loaded photocrosslinking GelMA/PEGDA scaffolds for periodontal bone regeneration.","authors":"Bei Gao, Jie Yao, Yijie Wang, Chen Chen, Zhen Chai, Wanting Wan, Shiyang Wu, Zhongbo Liu, Rui Zou","doi":"10.1186/s12896-026-01127-z","DOIUrl":"10.1186/s12896-026-01127-z","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel method to encapsulate polydeoxyribonucleotides extracted from trout into exosomes and applications in skin recovery. 从鳟鱼中提取的多脱氧核糖核苷酸包封到外泌体的新方法及其在皮肤恢复中的应用。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-17 DOI: 10.1186/s12896-026-01131-3
Yunhee Chang, Jun-Young Park, Dasom Hwang, Min Tae Kim, Seyeon Yoon, Seung-Hak Cho
{"title":"A novel method to encapsulate polydeoxyribonucleotides extracted from trout into exosomes and applications in skin recovery.","authors":"Yunhee Chang, Jun-Young Park, Dasom Hwang, Min Tae Kim, Seyeon Yoon, Seung-Hak Cho","doi":"10.1186/s12896-026-01131-3","DOIUrl":"10.1186/s12896-026-01131-3","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustainable utilization of phosphogypsum in agriculture enhanced by mycorrhizal organisms: a new perspective. 菌根菌促进磷石膏在农业中的可持续利用：一个新的视角。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-17 DOI: 10.1186/s12896-026-01134-0
Li Zhang, Juan Luo, Chuanguang Zhang, Hong Wang, Mingjie Yan, Naiming Zhang, Yunsheng Xia, Xianrong Yue
{"title":"Sustainable utilization of phosphogypsum in agriculture enhanced by mycorrhizal organisms: a new perspective.","authors":"Li Zhang, Juan Luo, Chuanguang Zhang, Hong Wang, Mingjie Yan, Naiming Zhang, Yunsheng Xia, Xianrong Yue","doi":"10.1186/s12896-026-01134-0","DOIUrl":"10.1186/s12896-026-01134-0","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Breaking the barrier of interspecific recalcitrance: a unified and efficient in vitro conservation system for Passiflora germplasm. 打破种间抗性障碍：一种统一高效的西番莲种质资源离体保护体系。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-16 DOI: 10.1186/s12896-026-01123-3
Wenting Xing, Dongmei Huang, Bin Wu, Funing Ma, Haijie Huang, Shun Song, Yi Xu
{"title":"Breaking the barrier of interspecific recalcitrance: a unified and efficient in vitro conservation system for Passiflora germplasm.","authors":"Wenting Xing, Dongmei Huang, Bin Wu, Funing Ma, Haijie Huang, Shun Song, Yi Xu","doi":"10.1186/s12896-026-01123-3","DOIUrl":"10.1186/s12896-026-01123-3","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ononin-modified Chitosan/β-glycerophosphate hydrogel-loaded adipose-derived stem cells promote the repair of diabetic wounds in rats. 水草素修饰的壳聚糖/β-甘油磷酸酯水凝胶负载脂肪干细胞促进大鼠糖尿病伤口的修复。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-14 DOI: 10.1186/s12896-026-01135-z
Fang Xu, Jian-Chao Peng, Yi-Ling Li, Zhi-Xin Huang, Meng-Jun Teng, Cui-Liu Gan, Ting Liao
{"title":"Ononin-modified Chitosan/β-glycerophosphate hydrogel-loaded adipose-derived stem cells promote the repair of diabetic wounds in rats.","authors":"Fang Xu, Jian-Chao Peng, Yi-Ling Li, Zhi-Xin Huang, Meng-Jun Teng, Cui-Liu Gan, Ting Liao","doi":"10.1186/s12896-026-01135-z","DOIUrl":"10.1186/s12896-026-01135-z","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei. 从干酪乳杆菌中提取的小肽触发p53介导的肺癌细胞系A549的细胞毒性和DNA损伤。
IF 3.4 3区 生物学
BMC Biotechnology Pub Date : 2026-03-13 DOI: 10.1186/s12896-026-01119-z
Jannatul Firdous Siddique, G R Shree Kumari, Sudarshana Deepa Vijay Kumar, C Subathra Devi, Mohanasrinivasan Vaithilingam
{"title":"p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei.","authors":"Jannatul Firdous Siddique, G R Shree Kumari, Sudarshana Deepa Vijay Kumar, C Subathra Devi, Mohanasrinivasan Vaithilingam","doi":"10.1186/s12896-026-01119-z","DOIUrl":"10.1186/s12896-026-01119-z","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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