BMC Biotechnology最新文献

{"title":"Lactococcus lactis I7 isolated from traditional Italian cheese making: a biotechnological integrated platform.","authors":"Alberto Alfano, Darshankumar Parecha, Alessandra Fusco, Vittoria Savio, Ida De Chiara, Lidia Muscariello, Giovanna Donnarumma, Chiara Schiraldi","doi":"10.1186/s12896-025-00997-z","DOIUrl":"10.1186/s12896-025-00997-z","url":null,"abstract":"<p><strong>Background: </strong>There is growing interest in newly isolated lactic acid bacteria from traditional sources. In this study, a Lactococcus lactis strain identified and isolated from natural whey starter cultures of cow milk for the production of artisanal cheeses was cultivated in optimized vegan grade medium to assess its growing ability and metabolic fingerprint. In fact, traditionally fermented dairy products are considered nutritionally complete and possibly functional to human health with a number of benefits not only to the gastro-intestinal tract but also in a systemic manner.</p><p><strong>Results: </strong>In fed batch experiments, we achieved 14 g/L dry cell weight and 1.9∙10¹⁰ viable colony forming unit increasing the values of experiment threefold and twofold respect to the batch processes, respectively. Additionally, the lactic acid (LA) production was quantified, and a maximal concentration of 78 g/L was achieved, which is approximately five-fold corresponding to the batch experiment results. This is in agreement with kinetic modeling of LA inhibition studies that highlighted a halved growth rate (µ) at 35 ± 5 g/L of LA whilst the growth blockage occurred at about 80 g/L. The samples obtaining after ultrafiltration processes and tested on three different pathogens, Enterococcus faecalis, Salmonella enterica subsp. enterica serovar Typhimurium and enteroinvasive Escherichia coli, gave a reduction on viability by 6-9 log on average.</p><p><strong>Conclusion: </strong>This paper focused its attention on optimization of fermentation conditions (in particular a vegan grade media using Design of Experiment approach) using fed-batch and microfiltration processes increasing the production of biomass and bioactive molecules, with respect to the batch processes. At the end of the fed-batch fermentation, a downstream process based on membranes was performed in order to obtain bioactive molecules that proved antimicrobial activity against intestinal/food spoilage pathogens.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"62"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mother transformer: A High-Throughput, Cost-Effective in Planta Hairy Root Transformation Method for Cannabis.","authors":"Ladan Ajdanian, Davoud Torkamaneh","doi":"10.1186/s12896-025-00990-6","DOIUrl":"10.1186/s12896-025-00990-6","url":null,"abstract":"<p><strong>Background: </strong>Hairy root (HR) transformation assays mediated by Agrobacterium rhizogenes, both in vitro and ex vitro, are essential tools in plant biotechnology and functional genomics. These assays can be significantly influenced by various factors, which ultimately can enhance the efficiency. In this study, we optimized a two-step ex vitro HR transformation method using the actual mother plant combined with the RUBY system and compared with existing methods.</p><p><strong>Results: </strong>The two-step ex vitro method proved more efficient than both the one-step ex vitro and in vitro methods, with the highest transformation efficiency of 90% observed in the actual plant. This technique also demonstrated a faster and less complicated approach, reducing time to achieve massive transgenic HR formation by 9-29 days compared to other methods.</p><p><strong>Conclusions: </strong>A novel, quicker, less complicated, and more efficient two-step transformation method for cannabis has been established, presenting a significantly lower risk of contamination. This protocol is particularly interesting to produce secondary metabolites using the CRISPR/Cas system in cannabis. We anticipate that this method will facilitate substantial time savings by rapidly producing hundreds of transformed samples.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"60"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the potential of biofabricated Ananas comosus peel selenium nanoparticles for antibacterial, antibiofilm, suppression of virulence genes (can and LuxS), anticancer, and antioxidant properties.","authors":"Mohamed K Y Soliman, Salem S Salem","doi":"10.1186/s12896-025-00999-x","DOIUrl":"10.1186/s12896-025-00999-x","url":null,"abstract":"<p><p>This research employed Ananas comosus (pineapple) peel waste (PPW) extract for producing selenium nanoparticles (SeNPs) using an ecologically feasible way, aimed at various medical uses. Our analysis demonstrated that the PPWextract was a significant supplier of several important phytochemicals. The synthesized SeNPs were comprehensively characterized via XRD, FTIR, SEM, EDX, UV-Vis, and HRTEM which exhibiting a spherical shape with dimensions between 33 and 73 nm. Additional experimental assessments of SeNPs were carried out to ascertain their suitability for usage in biology applications. The findings suggest that obtained SeNPs may effectively combat multiple bacteria, including S. aureus, E. coli, B. subtilis, E. faecalis, and K. pneumonia. Additionally, SeNPs exhibited antibiofilm capacity for both MRSA and E. coli with inhibition reported to be 64.8% and 54.4% at 100 µg/mL respectively. In the range of 62.5-31.25 µg/mL SeNPs reduced expression of two essential genes required for S. aureus to generate biofilms, cna (0.9 fold change), and quorum sensing gene LuxS of E. coli (4.2 folds of control to 3.4 folds of treated) in comparison to the RecA gene. The antioxidant capacity of SeNPs reported an IC₅₀ value of 98.3 µg/mL. The formed SeNPs demonstrated anticancer efficacy in combating the HepG2 malignant cell line, with an IC₅₀ value of 113.02 µg/mL.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"51"},"PeriodicalIF":3.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Metabolic engineering for microbial production of sugar acids.","authors":"Fatma Gizem Avci, Tim Prasun, Volker F Wendisch","doi":"10.1186/s12896-025-00996-0","DOIUrl":"10.1186/s12896-025-00996-0","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"50"},"PeriodicalIF":3.5,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells.","authors":"Abir M Shata, Mokhtar Saeed Rejili, Manal M El-Naggar, Samy A El-Asser, Ahmed A Saleh, Elsayed E Hafez, Faouzi Haouala, Amany S Youssef","doi":"10.1186/s12896-025-00991-5","DOIUrl":"10.1186/s12896-025-00991-5","url":null,"abstract":"<p><p>Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifungal properties. A promising new marine strain was isolated and identified as Streptomyces albidoflavus strain EgyAB2 (16 S rRNA gene sequence accession number ON680945.1). The anticancer activity of the extracted compounds was tested in the MCF7 cell line using a sulforhodamine B (SRB) bioassay, which revealed an IC₅₀ of 0.36 µg/ml compared to the chemotherapeutic drug 5-fluorouracil (0.35 µg/ml). Additionally, the anticancer activity was confirmed by a dimethyl-thiazol-diphenyl-tetrazolium bromide (MTT) bioassay, which showed an IC₅₀ of 17.46 µg/ml. The mode of action of the treated breast carcinoma (apoptotic effect) was studied via qRT-PCR, revealing a significant role in anticancer treatment. Although the extracted compounds exhibited high antioxidant activity in the diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, they presented an IC₅₀ of 8.92 µg/ml and an inhibition percentage of 56.08%. Chemical characterisation was performed via GC‒MS, 1 H-NMR, and FTIR spectroscopy analyses, revealing the presence of 2-D N-methyl imidazole, 2-nonadecene, 1-D-2-methyl imidazole, and propane dinitrile, all of which exhibit antitumour activity.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"48"},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration.","authors":"Heidi M Abdel-Mageed, Nermeen Z AbuelEzz, Ahmed A Ali, Amira Emad Abdelaziz, Dina Nada, Sahar M Abdelraouf, Shahinaze A Fouad, Abeer Bishr, Rasha A Radwan","doi":"10.1186/s12896-025-00989-z","DOIUrl":"10.1186/s12896-025-00989-z","url":null,"abstract":"<p><p>Effective treatment of skin wounds remains a clinical challenge owing to factors such as microbial infections, impaired fibroblast activity, disrupted angiogenesis, and collagen remodeling. In this study, we developed and evaluated novel curcumin-cyclodextrin hybrid nanoparticles (Cur/CD-HNPs) as a multifunctional platform for enhanced wound healing. Nanoparticles (NPs) were prepared via nanoprecipitation. Physicochemical and structural properties were systematically characterized by determining the encapsulation efficiency (EE), particle size, zeta potential, X-XRD, FTIR, SEM, in vitro release, and stability studies. The optimized Cur/CD-HNPs demonstrated a uniform particle size of 150.5 ± 2.8 nm, a surface charge of - 18.5 ± 0.59 mV, a PDI of 0.20 ± 0.03, and a high EE (90.2 ± 2.35%). Cur/CD-HNPs exhibited potent anti-inflammatory effects (97.93 ± 1.24% inhibition of protein denaturation), full antioxidant activity (100% ABTS radical scavenging, IC50 = 12.85 µg/mL), and broad-spectrum antibacterial efficacy. Cur/CD-HNPs exhibited a sustained biphasic release profile, with ~ 82% of Cur released over 24 h, supporting sustained delivery for wound healing applications. In vitro scratch assays revealed enhanced fibroblast proliferation and migration. For in vivo evaluation, the nanoparticles were incorporated into a hydrogel base and applied topically in a rat burn wound model, resulting in significantly accelerated wound closure (P < 0.05). Histopathological examination revealed improved epithelialization, collagen deposition, and tissue regeneration compared with the control groups. Our findings presented Cur/CD-HNPs as a promising therapeutic approach, offering Cur enhanced bioactivity, stability, and regenerative potential. This formulation addresses the key limitations of curcumin and presents a multifunctional and strong translational platform for clinical wound care.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"49"},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary compounds activates sirtuins - a computational modeling approach towards the management of ageing.","authors":"Nail Besli, Mazhar T Kaya, Nilufer Ercin, Ulkan Celik","doi":"10.1186/s12896-025-00985-3","DOIUrl":"10.1186/s12896-025-00985-3","url":null,"abstract":"<p><p>In biogerontology, researchers are actively exploring the manipulation of sirtuin proteins, a family of enzymes that play a crucial role in regulating lifespan and age-related diseases through bioactive compounds found in the diet. This report explores potential new insights by leveraging the innovative intersection of natural bioactive compounds and sirtuin modulation through computational drug design. Using state-of-the-art in silico techniques, including high throughput virtual screening (HTVS) and molecular dynamics (MD) simulation, the study identifies natural compounds from various dietary sources for sirtuin proteins (SIRT 1, 3, 6, 7) related to the cellular process in aging. HTVS was automated with the AutoDock-VINA to pinpoint the novel candidate bioactive compounds for sirtuins, while the MD was conducted with GROMACS. The top hits, fagopyrine, emblicanin B, epicatechin-(4beta- > 8)-epicatechin-(2beta- > 7,4beta- > 8)-catechin (epicatechin), withanolide, and dulcisisoflavone, had superior docking scores ranging from - 12.7 to - 7.2 kcal/mol. The MD displayed steady interactions between target proteins and biocompounds throughout 100 ns without significant conformational shifts. This paper contributes significantly to anti-aging research by presenting a novel, computationally efficient approach to discovering and developing sirtuin-modulating compounds. Highlighting the untapped potential of natural bioactive substances in modulating key biological pathways associated with aging opens new avenues for future experimental exploration of diet-based therapeutic strategies that may support healthy aging.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"47"},"PeriodicalIF":3.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycosynthesis of silver nanoparticles by Aspergillus templicola OR480102: a multifaceted approach for antibacterial, anticancer, and scratch assay applications.","authors":"Marwa M Abdel-Kareem, Maysa M A Ali, Abd El-Latif Hesham, Hossam E F Abdel-Raheam, Marwa Obiedallah","doi":"10.1186/s12896-025-00982-6","DOIUrl":"10.1186/s12896-025-00982-6","url":null,"abstract":"<p><strong>Background: </strong>Regarding their distinct physico-chemical and bioactivity characteristics, silver nanoparticles 'AgNPs' are extensively utilized in numerous scientific purposes.</p><p><strong>Results: </strong>Within this current investigation, for the first time, we evaluated how the extracellular extract of the isolate MAK223 generated exceptionally fixed AgNPs. The isolate was genetically identified as Aspergillus templicola OR480102. The generated AgNPs' physico-chemical characteristics were assessed using ultraviolet-vis spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared spectrometry (FT-IR). The maximum absorption in the UV-vis spectrum was obtained at 420 nm, matching the silver nanoparticles' surface plasmon absorbance. A. templicola OR480102 produced uniformly dispersed AgNPs between 5 and 25 nm with a mean dimension of 17.78537 ± 1.36 nm using TEM. FT-IR analysis identified functional groups (e.g., -OH, C = O) in the fungal filtrate that mediate AgNP synthesis and capping. To verify AgNPs stability, the dynamic light scattering (DLS) approach is employed. Optimal conditions for AgNPs synthesis were 10 days of incubation, one mM silver nitrate concentration, pH 11, and elevated temperatures. AgNPs demonstrated efficacy against clinically relevant pathogens: S. typhimurium 'ATCC 14028', B. subtilis 'ATCC 6633', S. aureus 'ATCC 25923', and E. coli 'ATCC 29213' were used in the study. Also, using AgNPs derived from the filtrate of A. templicola OR480102 shows significant potential as a novel therapeutic approach against breast cancer cells 'MCF-7'. The scratch assay of 'MCF-7' cells demonstrates the suppressive impact of AgNPs for these cell lines during proliferation by promoting apoptosis and reducing cell migration.</p><p><strong>Conclusion: </strong>Based on physico-chemical characteristics of AgNPs' and their antimicrobial and anticancer activities, it cleared that the selected strain Aspergillus templicola OR480102 is a promising producer of stable AgNPs' with significant bioactivities which could be applicable in different fields.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"46"},"PeriodicalIF":3.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells.","authors":"Ruolan Xin, Jiawen Zhang, Yinghui Zhang, Daoming Su, Menglin Li, Dan Liu, Ruizhi Wu, Junjie Zhao, Yuanyuan Zhu, Yebing Liu, Xiaoyun Chen, Yongjun Wen, Zhen Zhu","doi":"10.1186/s12896-025-00966-6","DOIUrl":"10.1186/s12896-025-00966-6","url":null,"abstract":"<p><strong>Background: </strong>Lumpy skin disease (LSD), caused by infection with the lumpy skin disease virus (LSDV), is a highly infectious disease that poses a notable challenge to the cattle industry worldwide. To conduct epidemiological monitoring of LSDV infection in cattle and evaluate the immune efficacy of LSDV vaccines, it is essential to develop a rapid, sensitive, and specific ELISA-based antibody detection method.</p><p><strong>Results: </strong>We utilized the LSDV P32 protein, stably expressed in a CHO-K1 suspension cell system, as a coating antigen to develop an indirect ELISA for Capripoxvirus (CaPV) antibody detection. This method specifically recognizes CaPV-positive sera without cross-reactivity with sera positive for bovine viral diarrhea virus, bovine rotavirus, infectious bovine rhinotracheitis virus, and Brucella antibodies. The method demonstrated a maximum serum dilution detection capacity of 1:3200, with intra- and inter-assay variation coefficients below 10%. Comparison with a commercially available kit showed an agreement of 95.7%.</p><p><strong>Conclusion: </strong>The indirect ELISA antibody detection method established exhibited excellent specificity, sensitivity, and reproducibility, providing a reliable tool for clinical detection and epidemiological surveys of LSDV. This method offers significant potential for the prevention and control of LSD outbreaks.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"45"},"PeriodicalIF":3.5,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexamethasone-boosted mesenchymal stem cell secretome: insight into hepatic protection.","authors":"Eiman M Adly, Thoria Diab, Mohamed Hessien","doi":"10.1186/s12896-025-00980-8","DOIUrl":"10.1186/s12896-025-00980-8","url":null,"abstract":"<p><p>As the independent anti-inflammatory effects of mesenchymal stem cells (MSCs) and glucocorticoids are well documented, it is hypothesized that the conditioned media derived from dexamethasone (DEXA)-treated MSCs may exhibit a potent therapeutic effect. To explore this, bone marrow-derived MSCs were transiently maintained in DEXA-containing media, where cell viability, phenotype, and osteogenic differentiation were assessed. Furthermore, the MSC-conditioned media (MSC-CN) was utilized to inhibit the proliferation of hepatoma cells and treat drug-induced acute liver failure (ALF) in mice. We found that low doses of DEXA (≤100 nM) maintained MSC viability and their typical mesenchymal phenotype. Conversely, 1000 nM decreased the expression of the mesenchymal markers (CD105 and CD90), triggered osteogenic differentiation as evidenced by the modulation of osteogenesis-related genes (alkaline phosphatase, osteopontin, and Runt-related transcription factor 2), and increased the intracellular calcium, assessed by Alizarin Red S staining. Moreover, MSC-DEXA-S restricted colony formation, cell migration, and glucose consumption in hepatoma cells. In parallel, MSC-DEXA-S protected mice against acetaminophen-induced ALF, where both liver functions, oxidative stress (Nrf-2, SOD1, GSH, and MDA), angiogenic (VEGF), and inflammatory (TNF-α) markers were improved. Also, MSC-DEXA-S resolved liver necrosis one week after transfusion. These data suggest that pretreatment of MSCs with low doses of dexamethasone maintains their stemness and enhances their paracrine therapeutic effect against hepatic diseases.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"44"},"PeriodicalIF":3.5,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}