Biological Control最新文献

{"title":"Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana","authors":"Qian Nong ,&nbsp;Yan Zhang ,&nbsp;Yanyan Long ,&nbsp;Yanlu Chen ,&nbsp;Liping Qin ,&nbsp;Shanyu Lin ,&nbsp;Fenghua Zeng ,&nbsp;Ling Xie","doi":"10.1016/j.biocontrol.2025.105781","DOIUrl":"10.1016/j.biocontrol.2025.105781","url":null,"abstract":"<div><div>Banana wilt-like disease, triggered by <em>Fusarium oxysporum</em>, poses a severe threat to banana cultivation as a persistent soilborne pathogen. A strategy to effectively control this disease has yet to be found. Dark septate endophytes (DSEs) fungi have emerged as promising biocontrol agents, not only offering protection against plant pathogens but also enhancing plant growth. Herein, we isolated a novel DSEs species, <em>Acidomelania saccharicola</em> LZ3, from sugarcane (<em>Saccharum officinarum</em>) soil in Liuzhou, Guangxi Province, China. Its classification as a new species was confirmed through morphological characterization and DNA sequence analysis. <em>A. saccharicola</em> LZ3 promoted the growth of banana and inhibited the growth of <em>F. oxysporum</em> by 73.28% in a Petri dish assay, and reduced disease by 52.82% in pots. The average incidence rate of Banana-LZ3 symbionts transplanted into a field highly contaminated with <em>F. oxysporum</em> was 25.93% and the control effect was 53.27%. Inoculation of banana plants with <em>A. saccharicola</em> LZ3 significantly increased polyphenol oxidase (POD) and superoxide dismutase (SOD) activity. This research lays the groundwork for future investigations into <em>A. saccharicola</em> and other DSEs, exploring their potential in sustainable plant disease management.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105781"},"PeriodicalIF":3.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris","authors":"Moon Joo Kim ,&nbsp;Eun-Jae Park ,&nbsp;Bori Lee ,&nbsp;Hyun-Jae Jang ,&nbsp;Jongmin Ahn ,&nbsp;Ji-Hye Kim ,&nbsp;Young Ho Kim ,&nbsp;Bong-Sik Yun ,&nbsp;Seung Woong Lee","doi":"10.1016/j.biocontrol.2025.105775","DOIUrl":"10.1016/j.biocontrol.2025.105775","url":null,"abstract":"<div><div><em>Xanthomonas campestris</em> pv. <em>campestris</em> (Xcc) is a gram-negative bacterium that causes black rot, a disease that significantly reduces cruciferous crops yields. Crop production is facing environmental and health challenges associated with the use of synthetic chemicals, highlighting the need for effective biological control agents as sustainable alternatives. In this study, we isolated and characterized the <em>Bacillus velezensis</em> 21–128 strain for antimicrobial activity against Xcc using pot experiments and various enzymatic activity assays. We used ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis to confirm the presence of lipopeptides, including surfactins in the ethyl acetate extract from <em>Bacillus velezensis</em> 21–128. Four types of surfactins—C13, C14, C15, and C16—were identified by mass fragmentation analysis. Further, we used a combination of separation methods, including HP-20 column chromatography, MPLC, and semi-preparative HPLC to isolate and purify the identified surfactins. The purified surfactins were compared with standard substances using HPLC and identified through comparative analysis with LC-MS data. Lastly, we evaluated the antimicrobial activity of the isolated surfactins against the Xcc strain, and determined the minimum inhibitory concentration values for the total surfactin group, G2, G3, G4, and G5. These results suggest that the <em>Bacillus velezensis</em> strain 21–128 could serve as a biological control agent against Xcc and could be a promising strategy against black rot in cruciferous crops.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105775"},"PeriodicalIF":3.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol activity of novel and known bioproducts based on Bacillus strains and basic products against Botrytis cinerea in tomato fruit","authors":"Salvina Panebianco,&nbsp;Silvia Americo,&nbsp;Angela Roberta Lo Piero,&nbsp;Gabriella Cirvilleri","doi":"10.1016/j.biocontrol.2025.105780","DOIUrl":"10.1016/j.biocontrol.2025.105780","url":null,"abstract":"<div><div>The use of bioproducts based on native microorganisms improves pre- and postharvest disease control by enhancing adaptability during storage and colonizing ability of fruit surface. This study aims to evaluate the effectiveness of three tomato-isolated <em>Bacillus</em> strains (<em>B. cereus</em> 6C, <em>B. thuringiensis</em> 18D and <em>B. velezensis</em> 23A) and of three commercial bioproducts based on <em>B. amyloliquefaciens</em> subsp. <em>plantarum</em> D747, chitosan hydrochloride and <em>Equisetum arvense</em>, alone and in mixture, in controlling <em>B. cinerea</em> on tomato fruit, investigating the possible mechanisms of action involved in the biocontrol activity of bacterial strains. All the tested strains reduced disease incidence and severity by more than 60 % when applied 3 days before pathogen inoculation, in both wound- and dip-treatments, showing performance similar or better than commercial bioproducts. <em>B. velezensis</em> 23A showed the best performance and persistence through time, even when applied 10 days before pathogen challenging. Monitoring of <em>Bacillus</em> population dynamics during tomato storage revealed good survival of the antagonists. <em>Bacillus</em> strains were able to secrete toxic metabolites and enzymes responsible for cell wall degradation. Conversely, the expression of tomato genes involved in salicylic acid (SA) and jasmonic acid (JA) signaling, as well as in pathogenesis related proteins (PRs) biosynthesis, was not induced by <em>B. velezensis</em> 23A. Taken together, the results highlighted that the ability of bacterial strains to colonize the host surface for long periods and to secrete toxic metabolites and enzymes is probably the main factor on which the level of disease control depends. Moreover, this study provides the basis for future developing carposphere-competent bio-inoculants to be used in preharvest for an ecofriendly management of postharvest grey mold.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105780"},"PeriodicalIF":3.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genome and transcriptome analysis of the endophytic fungus Epichloë sibirica reveals biological control mechanism in host Achnatherum sibiricum","authors":"Xinjian Shi ,&nbsp;Tianzi Qin ,&nbsp;Yubao Gao ,&nbsp;Nianxi Zhao ,&nbsp;Anzhi Ren","doi":"10.1016/j.biocontrol.2025.105778","DOIUrl":"10.1016/j.biocontrol.2025.105778","url":null,"abstract":"<div><div>Endophytic fungi of the genus <em>Epichloë</em> can bolster the host’s immune defenses against phytopathogens. However, the underlying mechanisms by which the endophytes regulate host responses remain poorly understood. This study elucidates the tripartite strategy employed by <em>Epichloë sibirica</em> to protect <em>Achnatherum sibiricum</em> through comparative genomics and transcriptomics. First, genomic and transcriptomic analyses revealed that <em>E. sibirica</em> can synthesize antibiotics (e.g., ε-poly-L-lysine) and mycelial degrading enzymes. Second, ecological niche occupation was supported by (1) minimal transcriptomic alterations in <em>E. sibirica</em> during pathogen challenge, (2) superior antimicrobial activity in fungal fermentation assays (superior antimicrobial activity of <em>E. sibirica</em> against four pathogens, while pathogen-derived broths had negligible effects on <em>E. sibirica</em> growth), and (3) comparative antibiotic susceptibility testing demonstrated <em>E. sibirica</em>’s significantly higher tolerance to strobilurin A, tetracycline, streptomycin sulphate, and penicillin sodium compared to pathogens. Third, host resistance induction involved gene clusters encoding effector proteins, cell wall-modifying enzymes, and trehalose. These findings identify <em>E. sibirica</em> as a multifaceted biocontrol agent through metabolite-mediated antagonism, ecological niche occupation, and host priming. Our results provide omics-level evidence of endophyte-driven tripartite defense mechanisms.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105778"},"PeriodicalIF":3.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Action of Pythium oligandrum on Grapevine Trunk Diseases and its impact on microbial communities","authors":"Séverine Lopez ,&nbsp;Alexandre Chataigner ,&nbsp;Jessica Vallance ,&nbsp;Ahmed Taïbi ,&nbsp;Assia Dreux-Zigha ,&nbsp;Marie-Cécile Dufour","doi":"10.1016/j.biocontrol.2025.105779","DOIUrl":"10.1016/j.biocontrol.2025.105779","url":null,"abstract":"<div><div>Grapevine Trunk Diseases (GTDs) have become a major challenge for viticulture. Since the ban of sodium arsenate (the only approved pesticide) in 2001, the need for alternative methods, such as biocontrol, has become increasingly urgent. One promising microorganism is the oomycete <em>Pythium oligandrum,</em> which is known to improve plant health by increasing natural defences and reducing disease incidence by up to 40 %. In order to use this microorganism in vineyards, it need first to be formulated and tested to ensure its safety. An experiment was therefore carried out in a greenhouse under semi-controlled conditions to assess the efficacy of the <em>P. oligandrum</em> biopesticide produced and its impact on microbial communities using a high-throughput sequencing approach. Vines were treated with the biopesticide and were inoculated with two fungi involved in wood diseases: <em>Neofusicoccum parvum</em> (involved in <em>Botryosphaeria</em> dieback) and <em>Phaeomoniella chlamydospora</em> (involved in Esca). During a three-month experiment, samples of the rhizosphere environment were collected to assess possible changes in microbial communities, either as part of GTDs or as a result of the action of <em>P. oligandrum</em>. The results indicated a minimal effect of the <em>P. oligandrum</em> biopesticide on the total microbial community of the vine rhizosphere. However, the treatment appeared to enhance several beneficial taxa that play a role as plant growth promoting rhizobacteria (PGPR) or biocontrol agents (BCA). This improvement, together with the direct effect of <em>P. oligandrum</em>, may explain the reduction in necroses caused by <em>N. parvum</em> and <em>P. chlamydospora</em> following the biopesticide application.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105779"},"PeriodicalIF":3.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type V: A sex-linked crossed resistance to three Cydia pomonella granulovirus isolates, with different levels of dominance","authors":"Léa Gingueneau ,&nbsp;Bertrand Gauffre ,&nbsp;Miguel Lopez-Ferber ,&nbsp;Sandrine Maugin ,&nbsp;Christine Blachère-Lopez ,&nbsp;Sofian Renoult ,&nbsp;Samantha Besse ,&nbsp;Myriam Siegwart","doi":"10.1016/j.biocontrol.2025.105777","DOIUrl":"10.1016/j.biocontrol.2025.105777","url":null,"abstract":"<div><div>The codling moth (<em>Cydia pomonella)</em>, is known to be resistant to many (bio)insecticides, including the <em>Cydia pomonella granulovirus</em> (CpGV). Of the multiple isolates available on the European market, two are already known to have selected resistance: Type I to CpGV-M and type II or III to group E (CpGV-S or -R5). In 2019, a monitoring program revealed three wild populations of insects with reduced susceptibility to the three main used virus isolates (CpGV-M, -R5 and -V15). In this context, our aim was to characterise this new type of resistance, referred to as Type V, and to determine its inheritance pattern and degree of dominance, and to investigate whether and how it differs from previously described resistance mechanisms.</div><div>We collected a population previously identified as multiresistant from which several lines were isolated and selected during 13 generations with either CpGV-M, -R5 or no virus. Bioassays clearly showed high levels of resistance to CpGV-M, -R5 and -V15, as well as cross-resistance between the three isolates. Individual crossing experiments showed different inheritance modes for resistance to CpGV-M and CpGV-R in type V. Resistance to CpGV-M appeared dominant Z-linked as in type I, while it was more likely recessive, but still Z-linked for resistance to CpGV-R. Altogether, Type V is a novel type of resistance that cannot be overcome by CpGV-V15, and marked by a distinct inheritance pattern. Further research is needed to understand the relationship between resistance to each isolate, and adapt the future pest management strategies.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105777"},"PeriodicalIF":3.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated the rhizosphere soil microbiota and metabolome reveal mechanisms inhibiting Phytophthora nicotianae under long-term bio-organic fertilizer application","authors":"Xiaoting Li ,&nbsp;Shunping Yu ,&nbsp;Shiqing Zhang ,&nbsp;Yubin Xiong ,&nbsp;Fangfang Zhou ,&nbsp;Li Tang","doi":"10.1016/j.biocontrol.2025.105774","DOIUrl":"10.1016/j.biocontrol.2025.105774","url":null,"abstract":"<div><div>Rational bio-organic fertilizer application has important advantages in reducing chemical fertilizer application, mitigating environmental pollution risks and enhancing plant health. However, the interplay between rhizosphere soil microbial communities, associated metabolites and the inhibiting pathogenic bacteria mechanisms is required, particularly in the long-term bio-organic fertilizer application. Therefore, our study encompassed three experimental conditions: conventional chemical fertilizer application (CF), 20 % reduction in chemical fertilizer supplemented with organic fertilizer (OF) and 20 % chemical fertilizer reduction supplemented with bio-organic fertilizer (BOF). By integrating microbiology and metabonomics, we aimed to elucidate the effects of long-term different fertilizer measures on the rhizosphere soil microbial community and metabolic function. Compared with CF, the relative abundance of <em>Phytophthora nicotianae</em> in rhizosphere soil and corresponding disease significantly decreased with BOF. However, there were significant increases in the concentrations of nitrate nitrogen, total nitrogen, available phosphorus, organic matter and urease activity. Simultaneously, the relative abundance of Proteobacteria at the phylum level notably increased with BOF, whereas the relative abundance of <em>Gemmatimonas</em> and <em>Sphingomonas</em> at the genus level exhibited significant increases. Moreover, the BOF affected the complexity and stability of the soil bacterial symbiotic networks. Furthermore, the metabolite profiles were significantly altered, with the differential metabolites in KEGG metabolic pathways being notably enriched for BOF treatment, particularly in pathways related to gibberellin secondary metabolite, histidine, and tryptophan metabolism. The correlation analysis and structural equation modeling revealed significant interactions between soil properties, microorganisms and metabolites, all of which had a substantial impact on the disease incidence. Consequently, we observed that soil fertility, rhizosphere microorganisms and tryptophan metabolites collectively facilitated the favorable response of crop health to the partial replacement of chemical fertilizers with bio-organic alternatives. These findings provided novel insights into sustainable practices of the reduction of chemical fertilizer and environmental pollution.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105774"},"PeriodicalIF":3.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria-based artificial diets modulate larval development, survival and gut microbiota of two insect pests","authors":"A.O. Adesemoye ,&nbsp;S. Antony-Babu ,&nbsp;E.M. Nagy ,&nbsp;B.D. Kafle ,&nbsp;T.A. Gregory ,&nbsp;C. Xiong ,&nbsp;H.Y. Fadamiro","doi":"10.1016/j.biocontrol.2025.105769","DOIUrl":"10.1016/j.biocontrol.2025.105769","url":null,"abstract":"<div><div>Crop damage caused by insect pests results in substantial economic losses. The most widely used control methods, chemical pesticides, are losing efficacy and have deleterious environmental and health impacts. The goal of this study was to evaluate plant growth-promoting rhizobacteria (PGPR) strains blended as consortia for the management of insect pests through the impact on insect-gut microbiota. PGPR isolates were recovered from corn rhizosphere in Texas and characterized through 16S rRNA gene sequencing. Eight strains belonging to diverse taxa selected through prescreening were combined in three different blends (consortia), added into an insect artificial diet, and fed to larvae of corn earworm (<em>Helicoverpa zea</em> [Boddie, 1850]) and tobacco budworm (<em>Chloridae virescens</em> [Fabricius, 1777]). Insect growth and development was monitored, and gut microbiota was assessed through direct isolation and 16S rRNA sequencing approaches. The inoculated diet resulted in significant reduction in larval weight for both insect species compared to the control and caused changes in the taxonomic diversity and evenness of <em>H. zea</em> gut microbiota. The results of the isolation and 16S rRNA sequencing were generally in agreement and showed a higher population and dominance of bacilli but less diversity of other taxa in PGPR blend treatments compared to control. While the mechanism of the changes in gut microbiota is currently unknown, understanding how to utilize bacterial strains to manipulate insect’s gut microbiota and health is important for developing sustainable pest management strategies. Future studies should elucidate the mechanisms and how to effectively deploy these findings for field applications.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105769"},"PeriodicalIF":3.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Fusarium Biocontrol, drought Tolerance, and plant growth promotion by Bacillus strains from Cuban wheat varieties","authors":"Alexander Govin-Sanjudo ,&nbsp;Marcia M. Rojas Badia ,&nbsp;Cédric Jacquard ,&nbsp;Qassim Esmaeel","doi":"10.1016/j.biocontrol.2025.105776","DOIUrl":"10.1016/j.biocontrol.2025.105776","url":null,"abstract":"<div><div>Members of the Bacillaceae family are recognized for their ability to promote plant growth under drought stress and for their capability to produce bioactive metabolites displaying antagonistic activity against phytopathogenic fungi. In this study, we evaluated the potential of bacterial strains isolated from Cuban wheat accessions to inhibit <em>Fusarium</em> species, tolerate drought stress, and promote the germination of wheat under stress conditions. A total of 150 bacteria were isolated from three Cuban wheat accessions, and 15 isolates were selected based on their elevated antagonistic activity. These strains, belonging to the Bacillaceae family, effectively reduced <em>Fusarium</em> symptoms on wheat leaves. To determine tolerance to drought stress, the isolates were exposed to different concentrations of polyethylene glycol (PEG) −6000, showing tolerance to 20 % PEG-6000. Furthermore, the strains underwent <em>in vitro</em> evaluation for plant growth-promoting (PGP) and lytic enzymes production. Ammonia production, indoleacetic acid, amino cyclopropane carboxylate deaminase, and nitrogen fixation were detected in all bacteria; moreover, each strain produced at least two of the five lytic enzymes tested. The bacterial isolates promoted wheat germination under both drought stress and <em>Fusarium</em> infection; with the highest effects registered for strains TII-10, TII-19, TCG-6 and TMG-6. These findings highlight the potential of these strains as effective agents for the promotion of wheat growth under the dual stresses of <em>Fusarium</em> infection and drought, contributing to more sustainable wheat production.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105776"},"PeriodicalIF":3.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of the biological control of aphids by their natural enemies in sugar beet crops","authors":"Lena Barascou ,&nbsp;Marianne Doehler ,&nbsp;Ségolène Buzy ,&nbsp;Aurore Arnoult ,&nbsp;Eloi Salembier ,&nbsp;Valentin Richard ,&nbsp;Loïc Daniel ,&nbsp;Frank Duval ,&nbsp;Anne Le Ralec","doi":"10.1016/j.biocontrol.2025.105773","DOIUrl":"10.1016/j.biocontrol.2025.105773","url":null,"abstract":"<div><div>Conservation biological control system involves a diverse community of natural enemies that significantly regulate pests in crops. However, the relative contribution of these different functional groups of natural enemies to aphid regulation is poorly known in major crop-pest systems. Moreover, there is a need to identify the timing of this contribution, especially when aphids are able to transmit pathogenic viruses to plants, such as in sugar beet crops. In this study, we quantified the effect of ground dwelling predators (carabids, spiders, rove beetles) and parasitoids on aphids’ abundance in sugar beet crops, using two experiments conducted over two successive years (2022 and 2023). Based on physical exclusion devices (pitfall traps, barriers, insect-proof cages), we showed that ground dwelling predators significantly lowered the abundance of aphids (<em>Aphis fabae</em> and <em>Myzus persicae</em>). Ground dwelling predators made a significant contribution to natural pest control, as they lowered aphids’ populations by up to 80%, especially as they were present early in the season. Around 48% parasitism rate was measured in 2023. Our results suggest that both groups of natural enemies appear to play a particularly important role in aphid regulation, especially ground dwelling predators. This suggests that agricultural practices, as a vegetated field margin, favouring generalist predators overwintering or colonization could lead to fewer damage and losses for farmers.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105773"},"PeriodicalIF":3.7,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}