Biological Control最新文献

{"title":"Biological control of green mold in simulated post-harvest chain of citrus Fruit: Efficacy of Candida oleophila strain O and molecular insight into elicitation of host immune system","authors":"Ermes Ivan Rovetto ,&nbsp;Federico La Spada ,&nbsp;Soumia El boumlasy ,&nbsp;Sebastiano Conti Taguali ,&nbsp;Mario Riolo ,&nbsp;Antonella Pane ,&nbsp;Santa Olga Cacciola","doi":"10.1016/j.biocontrol.2024.105531","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105531","url":null,"abstract":"<div><p>Managing post-harvest decays in citrus fruit without relying on conventional pesticides presents a significant challenge in modern Plant Pathology. This study aimed to evaluate the efficacy of the biological control agent <em>Candida oleophila</em> strain O in controlling green mold caused by <em>Penicillium digitatum</em> throughout various stages of the post-harvest supply chain. Using a series of <em>in vivo</em> experiments, different scenarios of <em>P. digitatum</em> infections in clementine tangerine, orange, and lemon fruit were examined, with treatments applied before, during or after infection. The study simulated typical conditions of the citrus supply chain, including picking, processing in packinghouses, and transportation, as well as cold storage and shelf-life phases. Results indicated that <em>C. oleophila</em> exhibited significant efficacy in reducing green mold symptoms, even at shelf-life temperatures, making it a practical alternative to conventional fungicides. Additionally, the study provided insights into the molecular mechanisms underlying the defensive response of citrus fruit to <em>C. oleophila</em> treatment, with up-regulation of defense-related genes observed across different fruit types. Overall, this study underscores the potential of <em>C. oleophila</em> as a sustainable and effective solution for managing post-harvest decays in citrus fruit within the complexities of the supply chain.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105531"},"PeriodicalIF":4.2,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000963/pdfft?md5=0bbf5af537cf57c406933367762e2a6a&pid=1-s2.0-S1049964424000963-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141068625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics-driven host test list selection for weed biological control","authors":"Stephanie H. Chen ,&nbsp;Ben Gooden ,&nbsp;Michelle A. Rafter ,&nbsp;Gavin C. Hunter ,&nbsp;Alicia Grealy ,&nbsp;Nunzio Knerr ,&nbsp;Alexander N. Schmidt-Lebuhn","doi":"10.1016/j.biocontrol.2024.105529","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105529","url":null,"abstract":"<div><p>A concern in weed biological control research is the potential for candidate biocontrol agents to impact not only the target weed but also native or economically important flora. The degree of evolutionary relatedness between the target weed species and a non-target species is a key predictor of the susceptibility of the non-target to the biocontrol agent. To manage this risk, biocontrol practitioners need to understand the phylogenetic position of the host weed relative to non-target plant species. However, comprehensively sampled phylogenetic trees are often unavailable, with incomplete information scattered across multiple publications. Further, older published phylogenies based on Sanger sequence data often lack branch resolution and support, which increases uncertainty in biocontrol decision making. Decreasing sequencing cost and technological advances have led to phylogenomic approaches being more widely used to understand evolutionary relationships between species. For example, target capture sequencing methods using bait kits such as Angiosperms353 enable cost-effective and timely phylogenomic-level analysis of flowering plant groups at different scales. Here, we introduce a workflow to embed a comprehensive understanding of evolutionary relationships into the efficient development of host test lists in weed biological control. We demonstrate the effectiveness of the workflow through a case study on the major crop weed flaxleaf fleabane (<em>Erigeron bonariensis</em>). Phylogenomic analysis was conducted on 280 species of the tribe Astereae (family Asteraceae) occurring in Australia and New Zealand, clarifying relationships between the target species and related clades of native and non-native Astereae. We consider the phylogenetic tree in the context of a previously proposed host test list and discuss taxonomic implications, highlighting avenues of future molecular-based work to uncover the origin of Australian fleabanes. This study provides a workflow and demonstrates the practical application of target sequence capture for phylogenomic inference to support risk analysis and decision making in classical weed biological control.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105529"},"PeriodicalIF":4.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S104996442400094X/pdfft?md5=3ee4ac1ecb0195f1c80d5538e15d6a81&pid=1-s2.0-S104996442400094X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective biological control of chickpea rabies (Ascochyta rabiei) through systemic phytochemical defenses activation by Trichoderma roots colonization: From strain characterization to seed coating","authors":"Javier Morcuende ,&nbsp;Jorge Martín-García ,&nbsp;Pablo Velasco ,&nbsp;Tamara Sánchez-Gómez ,&nbsp;Óscar Santamaría ,&nbsp;Víctor M. Rodríguez ,&nbsp;Jorge Poveda","doi":"10.1016/j.biocontrol.2024.105530","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105530","url":null,"abstract":"<div><p>Chickpea (<em>Cicer arietinum</em>) is a legume of great economic and agricultural importance worldwide, whose crop is severely affected by rust or Ascochyta blight, caused by the fungus <em>Ascochyta rabiei</em>. The fungal genus <em>Trichoderma</em> includes several species widely characterized as effective biological control agents against crop pathogens. First, this work characterized several species of the genus <em>Trichoderma</em> as potential biological control agents of <em>A. rabiei</em> directly (<em>in vitro</em> confrontation) or indirectly in chickpea plants (activation of systemic resistance), selecting <em>T. harzianum</em> EN1 as the most efficient strain. Subsequently, different materials were tested as coatings to apply the <em>T. harzianum</em> conidia on chickpea seeds, determining that gum arabic at 1 % concentration was the one that most promoted the germination of conidia and seeds. The third phase of the study was based on the application of the coating and <em>T. harzianum</em> conidia on chickpea seeds and to study plant survival after infection with the pathogen <em>A. rabiei</em>, characterizing root colonization by <em>Trichoderma</em> and systemic hormonal and metabolic changes related to the induction of systemic defenses. The treatment of chickpea seeds with gum arabic and <em>T. harzianum</em> conidia was found to increase <em>Trichoderma</em>-root colonization and to improve plant survival. The induction of systemic ethylene- and melatonin-mediated resistance, which led to the accumulation of nicotinic acid in plant tissues, was considered the cause of such a protection. Therefore, <em>T. harzianum</em> applied as seed coating with gum arabic could be a good biological control strategy against <em>A. rabiei</em> on chickpea, due to the induction of systemic resistance.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105530"},"PeriodicalIF":4.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000951/pdfft?md5=fbd8eb65d2d988ded0a1fa46c9469e52&pid=1-s2.0-S1049964424000951-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide","authors":"Imen Ben Slimene Debez ,&nbsp;Amani Ben Alaya ,&nbsp;Ines Karkouch ,&nbsp;Bilel Khiari ,&nbsp;Pedro Garcia-Caparros ,&nbsp;Nouf M. Alyami ,&nbsp;Ahmed Debez ,&nbsp;Belhassen Tarhouni ,&nbsp;Naceur Djébali","doi":"10.1016/j.biocontrol.2024.105527","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105527","url":null,"abstract":"<div><p>Potato is a major crop whose yield and quality are impacted by the pathogenic fungus <em>Rhizoctonia solani</em>. Here, a control approach based on the use of endophytic bacillus spores and bacterial consortia was assessed as an innovative solution to improve tuber productivity and health. Endophytic potato bacteria extracted from several organs (leaves, stems and roots) evaluated for their antagonistic activity against <em>R. solani</em> by the double culture technique and their ability to rot potato slices. Additionally, the ability of Bacillus strains to generate hydrolytic enzymes and produce lipopeptides (using ESI-MS/MS mass spectrometry) was assessed. Then, the effectiveness of selected bacterial strains, either individually or in consortium (after carrying out compatibility tests) in the suppression of <em>R. solani</em> was evaluated in the greenhouse. Six endophytes not affecting potato tubers and having an antagonistic effect against <em>R. solani</em> were identified: <em>B. halotolerans</em> SpS5, <em>B. velezensis</em> KnL15, <em>B. aryabhattai</em> FaR1, <em>B. amyloliquefaciens</em> LiR9, <em>B. haynesii</em> ReR10 which produce several types and combinations of lipopeptides belonging to different families (iturins, fengycins and surfactins) as well as hydrolytic enzymes (chitinase, protease and cellulase). The vegetative and sporulating strain SpS5, as well as the 5 other Bacillus and their Sps5-based consortia, were found to be very effective in increasing the yield of healthy, high-quality tubers and reducing disease severity. Based on the results mentioned above, a microbial fungicide from the spores of (i) <em>B. halotolerans</em> SpS5 for which we sequenced the whole genome or (ii) bacterial consortia could be developed to combat black scurf potato and other fungal infections.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105527"},"PeriodicalIF":4.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000926/pdfft?md5=f76bac90d232fa31134f1b4b91097b9e&pid=1-s2.0-S1049964424000926-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unleashing nature's defenders: Farmer-managed natural enemies field reservoirs (NEFRs) enhance management of the invasive papaya mealybug (Paracoccus marginatus) in coastal Kenya","authors":"Stephen T.O. Othim ,&nbsp;Selpha Opisa ,&nbsp;Ivan Rwomushana ,&nbsp;Belinda Luke","doi":"10.1016/j.biocontrol.2024.105528","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105528","url":null,"abstract":"<div><p>The papaya mealybug (PMB), <em>Paracoccus marginatus</em>, infests a wide range of plant species, including economically important crops, like papaya, citrus, cassava, and avocado, leading to significant economic losses. The parasitoid, <em>Acerophagous papayae</em> has been shown to manage the pest and was introduced in three coastal counties of Kenya from 2021. Natural Enemies Field Reservoirs (NEFRs), a technology that serves as in-situ production of <em>A.papayae</em>, were established in farmers’ fields in the three counties to assess their effectiveness in controlling the papaya mealybug in Kenya. Three treatments were set up including a. ‘No prior <em>A. papayae</em> release + NEFR’, b. ‘Prior <em>A. papayae</em> release + NEFR’, and c. ‘Prior <em>A. papayae</em> release but no NEFR’ (control). PMB populations decreased by 49.12 % and 62.8 % in treatments a and b, respectively, but increased by 37.6 % in the control. On the other hand, the absolute count of <em>A. papayae</em> increased by 456 %, 190 % and 51.6 % in treatments a, b and c, the control, respectively. Consequently, the parasitism rates increased by 116.7 % and 17.8 % in treatments a, and b, respectively but declined by 10.3 % in the control. The most abundant predators out of ten recovered genera were <em>Cryptolaemus montrouzieri</em> (44.0 %)<em>, Tenuisvalvae notata</em> (23.9 %) and <em>Exochomus</em> sp. (19.9 %). The highest abundance and diversity of predators was recorded in treatment b ‘Prior <em>A. papayae</em> release + NEFR’ and the least abundance and diversity in the control. This study sheds light in the critical role of NEFRs in the management of PMB and its underscored potential as an effective, low-cost, farmer managed technology is discussed.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105528"},"PeriodicalIF":4.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000938/pdfft?md5=96371e77f2e227f85614bbde9d553655&pid=1-s2.0-S1049964424000938-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140947169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared patterns of population genomic variation and phenotypic response across rapid range expansions in two invasive lady beetle species","authors":"Angela G. Jones ,&nbsp;John J. Obrycki ,&nbsp;Arun Sethuraman ,&nbsp;David W. Weisrock","doi":"10.1016/j.biocontrol.2024.105519","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105519","url":null,"abstract":"<div><p>Non-native lady beetle species have often been introduced, with variable success, into North America for biological control of aphids, scales, whiteflies, and other agricultural pests. Two predatory lady beetle species, <em>Propylea quatuordecimpunctata</em> and <em>Hippodamia variegata</em>, both originating from Eurasia, were first discovered near Montreal, Quebec, in North America in 1968 and 1984, respectively, and have since expanded into northeastern North America and the midwestern United States. In this study, we estimate the range-wide population structure, establishment and range-expansion, and recent evolutionary history of these lady beetle species using reduced-representation genotyping-by-sequencing via ddRADseq. In addition, we quantified the responses to a key abiotic factor, photoperiod, that regulates adult reproductive diapause in these two species and may influence their geographical range in North America. Our analyses detect: (1) non-significant genetic differentiation and divergence among North American populations that likely originated from a single accidental introduction, (2) evidence of reduced contemporary gene flow within the continental US, and (3) minor phenotypic differences in diapause induction between populations of the two species from the same location.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105519"},"PeriodicalIF":4.2,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000847/pdfft?md5=a52abc368234e5a150a1b8915f717484&pid=1-s2.0-S1049964424000847-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141068624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting the combination of entomopathogenic fungi and Illicium verum essential oil against Aedes aegypti larvae","authors":"Iury Uzêda Rocha ,&nbsp;Ricardo de Oliveira Barbosa Bitencourt ,&nbsp;Aimée de Moraes Freitas ,&nbsp;Haika Victória Sales Moreira ,&nbsp;Kamila Leite de Amorim Magalhães ,&nbsp;Bianca Augusto de Souza ,&nbsp;Patrícia Silva Golo ,&nbsp;Douglas Siqueira de Almeida Chaves ,&nbsp;Vânia Rita Elias Pinheiro Bittencourt ,&nbsp;Isabele da Costa Angelo","doi":"10.1016/j.biocontrol.2024.105526","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105526","url":null,"abstract":"<div><p>Here, the effectiveness of <em>Beauveria bassiana</em> LCM S19 and <em>Metarhizium anisopliae</em> LCM S01, combined with <em>Illicium verum</em> essential oil (EO) against <em>Aedes aegypti</em> larvae was investigated. The EO compounds were first identified through gas chromatography, with the primary compound being <em>(E)-</em>anethole. Subsequently, <em>in silico</em> analysis was employed to predict the biological activity of <em>(E)-</em>anethole, revealing 89 molecular targets, which included insecticidal activity. Next the impact of EO on fungal viability and colony growth was examined and the EO had no effect on fungal development. Next, we investigated the influence of EO on fungal viability and colony growth, revealing that the EO had no discernible impact on fungal development. Following this, individual assessments were carried out to determine larval susceptibility to EO at concentrations of 40, 60, 80, and 100 ppm, as well as with both fungal isolates at conidial concentrations of 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ conidia/mL. Larval survival was then monitored over a period of seven days. To further optimize the larvicidal potential, EO at a concentration of 40 ppm was combined with both isolates at a conidial concentration of 1 × 10⁶ conidia/mL and tested against mosquito larvae, as described earlier. The combination of <em>M. anisopliae</em> and EO resulted in a substantial decrease, with only 20 % of larvae surviving over a seven-day period, in contrast to approximately 50 % survival observed in the pure <em>M. anisopliae</em> or EO treatments. <em>B. bassiana</em>, whether used alone or in combination with EO, exhibited a 50 % reduction in larval survival. The combination of <em>M. anisopliae</em> and EO exhibited a synergistic effect in targeting <em>Ae. aegypti</em> larvae, whereas <em>B. bassiana</em>, in conjunction with EO, had an additive effect. Notably, the combination achieved its impact in just 3 days, whereas the pure fungus required 7 days to yield similar results. These findings underscore the substantial potential of combining <em>I. verum</em> essential oil with both fungal isolates against <em>Ae. aegypti</em> larvae.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105526"},"PeriodicalIF":4.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000914/pdfft?md5=611e81538db44f6b163796579de974d7&pid=1-s2.0-S1049964424000914-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140894190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of heat-resistant blastospores by Cordyceps javanica IF-1106 through optimizing metal ions composition","authors":"Yihua Li ,&nbsp;Meiyu Gao ,&nbsp;Si Chen ,&nbsp;Hongliang Diao ,&nbsp;Wenwen Zhou ,&nbsp;Ruiyan Ma","doi":"10.1016/j.biocontrol.2024.105518","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105518","url":null,"abstract":"<div><p>Heat tolerance of fungi is critical for field stability and efficacy of fungal insecticides. Components of the medium, such as carbon source, nitrogen source and metal ions, contribute to the thermotolerance of fungi. In this study, several metal ions were detected to establish their effects on the sporulation and thermotolerance of the blastospores of <em>Cordyceps javanica</em> IF-1106 when cultured in liquid medium. The results showed that metal ions play different roles in sporulation and thermotolerance of the fungi. Mn²⁺ greatly increased the thermotolerance of the blastospores, and Fe²⁺ increased the thermotolerance slightly. However, both has no or negative effects on sporulation of the fungi. In the case of Cu²⁺ and Fe³⁺, sporulation yield and thermotolerance of the blastospores increased significantly. In addition, intracellular trehalose and mannitol involved in thermotolerance of fungi were extracted and then quantified by ion chromatography. We find that the trehalose and mannitol are higher in the presence of Mn²⁺, implying that Mn²⁺ could adjust the intracellular trehalose and mannitol level confers the blastospores with higher tolerance to thermal stress. Further, Response Surface Methodology (RSM) was applied to screen the optimal composition of metal ions. When the formula was MnSO₄·H₂O 8 mg/L, CuSO₄·5H₂O 5 mg/L, FeSO₄·7H₂O 11 mg/L, FeCl₃·6H₂O 8 mg/L, maximum sporulation yield and GT₅₀ were obtained. Lastly, the virulence of the blastospores to <em>Acyrthosiphon pisum</em> was validated, a higher virulence is achieved with decreased LT₅₀ (2 d) and LC₅₀ (2.37 × 10⁴ blastospores mL⁻¹). In conclusion, thermotolerant blastospores of <em>C. javanica</em> IF-1106 and higher sporulation yield could be achieved by optimizing the metal ions composition, which helps to determine the formula for larger scale fermentation process.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"193 ","pages":"Article 105518"},"PeriodicalIF":4.2,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000835/pdfft?md5=da8660b94e7003f86ec3ab27e31b78cc&pid=1-s2.0-S1049964424000835-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140817039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage","authors":"Laura Depalo ,&nbsp;Carolina Gallego ,&nbsp;Raúl Ortells-Fabra ,&nbsp;Carlos Salas ,&nbsp;Rafael Montalt ,&nbsp;Alberto Urbaneja ,&nbsp;Meritxell Pérez-Hedo","doi":"10.1016/j.biocontrol.2024.105517","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105517","url":null,"abstract":"<div><p>Although <em>Nesidiocoris tenuis</em> is highly effective as a biological control agent, it can also damage tomato plants due to its zoophytophagous behavior. When <em>N. tenuis</em> pierces the stems and petioles of tomato plants with its stylets, it triggers callose deposition and subsequent cell death, resulting in blocked nutrient transport, floral abortions, or wilting of tender shoots. Recently, it has been shown that exposure of tomato plants to the green leaf volatile (Z)-3-hexenyl propanoate [(Z)-3-HP] activates defensive mechanisms, including the regulation of genes involved in the synthesis and degradation of callose. In this study, conducted under greenhouse conditions, we tested the hypothesis that damage caused by <em>N. tenuis</em> could be reduced by exposing tomato plants to (Z)-3-HP through polymeric dispensers. Tomato plants exposed to (Z)-3-HP and non-exposed control plants were inoculated with <em>N. tenuis</em>. <em>Nesidiocoris tenuis</em> established in both groups with no significant differences between the two treatments. However, as hypothesized, the damage caused by <em>N. tenuis</em> was significantly lower in the plants exposed to (Z)-3-HP. Gene expression analysis of salicylic, jasmonic, and abscisic acids, along with histochemical staining methods, was used to compare the defensive responses of tomato plants infested solely with <em>N. tenuis</em> versus those infested with <em>N. tenuis</em> and exposed to (Z)-3-HP. Our findings confirm the influence of (Z)-3-HP exposure on differential defensive activation between treatments and reduced callose deposition in (Z)-3-HP-exposed plants. These results pave the way for improved management of <em>N. tenuis</em> by enhancing the plant's defenses based on inter-plant communication.</p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"192 ","pages":"Article 105517"},"PeriodicalIF":4.2,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000823/pdfft?md5=3b4d353956efb8c9d90739c3844248be&pid=1-s2.0-S1049964424000823-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140649601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective biological control of southern blight of Dendrobium officinale by Streptomyces sp. MEPF0303","authors":"Yu-Jie Chen ,&nbsp;Yong-Jing Zhang ,&nbsp;Qiao Liu ,&nbsp;Jun Dai ,&nbsp;Zhi-Li Jiang ,&nbsp;Hai-Lan Fu ,&nbsp;Shi-Yu Cai ,&nbsp;Ji-Hong Jiang ,&nbsp;Lu-Dan Li","doi":"10.1016/j.biocontrol.2024.105513","DOIUrl":"https://doi.org/10.1016/j.biocontrol.2024.105513","url":null,"abstract":"<div><p>Southern blight is a common and devastating disease adversely affecting the yield and quality of cultivated <em>Dendrobium officinale</em> plants. The pathogens responsible for this disease are <em>Sclerotium delphinii</em> and <em>S. rolfsii</em>, although <em>S. delphinii</em> is more pathogenic than <em>S. rolfsii</em>. Plant endophytes are potential sources of biological control agents. In this study, 18 microbial strains were isolated from wild <em>Dendrobium shixingense</em> plants (e.g., <em>Streptomyces</em>, <em>Bacillus</em>, and <em>Trichoderma</em>), among which strain MEPF0303 significantly inhibited <em>S. delphinii</em> mycelial growth. Based on 16S rRNA gene sequence, this strain was identified as <em>Streptomyces</em> sp. In addition, the volatile organic compounds and cell-free supernatant of MEPF0303 had significant inhibitory effects on <em>S. delphinii</em> growth. Moreover, MEPF0303 effectively controlled southern blight of <em>D. officinale</em>. A total of 1,797 differentially expressed genes were identified by a comparative transcriptome analysis of <em>S. delphinii</em> mycelia treated with MEPF0303 and the untreated control mycelia. Strain MEPF0303 significantly altered the expression of genes related to cell membrane morphological development and integrity, pathogenicity, energy metabolism, and oxidative stress responses. Furthermore, the transcriptome sequencing results were validated by analyzing gene expression via a quantitative real-time polymerase chain reaction. The study results showed that MEPF0303 can inhibit the growth and pathogenicity of <em>S. delphinii</em>, with potential implications for the biological control of southern blight of <em>D. officinale.</em></p></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"192 ","pages":"Article 105513"},"PeriodicalIF":4.2,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424000781/pdfft?md5=797ec2efe1740ff090dfcdcd1995e3fc&pid=1-s2.0-S1049964424000781-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}