Biological Control最新文献

{"title":"Latin America has the largest area under augmentative biological control worldwide, mainly with applications in open field crops","authors":"Joop C. van Lenteren ,&nbsp;Vanda H.P. Bueno ,&nbsp;Wagner Bettiol","doi":"10.1016/j.biocontrol.2025.105827","DOIUrl":"10.1016/j.biocontrol.2025.105827","url":null,"abstract":"<div><div>During the past 30 years, augmentative biological control (ABC) has received increasing interest and is now applied in a large diversity of crops on many hectares in Latin America and the Caribbean. Around the year 2000 4.35 million hectares were estimated to be treated with ABC, in 2018 this had grown to 31.4 million hectares, and the estimate for 2024 was about 62 million hectares. Many factors explain this dramatic increase, the most important being (a) the development of microbial agents that are relatively cheap compared to macrobial agents, easy to produce, store and apply, and reliable, (b) the fine tuning and drastic shortening of registration procedures, (c) a change in attitude towards use of alternatives for chemical pesticides among young farmers. However, there are also factors frustrating implementation of ABC of which the most important are the lobbying activities of the synthetic pesticide industry and lack of application of the true cost principle for these pesticides which makes them unrealistically cheap. Major differences with other world regions are that in Latin America ABC is almost exclusively applied in the open field, is often not based on development of resilient systems and changed in the 21st century from mainly using macrobial control agents to increased applications with microbial control agents.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105827"},"PeriodicalIF":3.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling Riptortus pedestris (Hemiptera: Alydidae) using Ooencyrtus utetheisae (Hymenoptera: Encyrtidae)","authors":"Tianjie Liu ,&nbsp;Yue Cui ,&nbsp;Yichang Xing ,&nbsp;Yu Gao ,&nbsp;Yuan Chen ,&nbsp;Shusen Shi ,&nbsp;Jinping Zhang","doi":"10.1016/j.biocontrol.2025.105829","DOIUrl":"10.1016/j.biocontrol.2025.105829","url":null,"abstract":"<div><div><em>Riptortus pedestris</em> (Hemiptera: Alydidae) is an important pest that affects soybean cultivation in East Asia. In the present study, the ability of <em>Ooencyrtus utetheisae</em> (Hymenoptera: Encyrtidae) to provide biological control of <em>R. pedestris</em> was evaluated in laboratory and field-cage experiments. The developmental time, longevity, reproductive rate, and functional response of <em>O. utetheisae</em> attacking <em>R. pedestris</em> eggs were measured. The influence of parasitism by <em>O. utetheisae</em> females of different ages was assessed for <em>R. pedestris</em> eggs of different ages. In addition, rate of parasitism of <em>R. pedestris</em> eggs by <em>O. utetheisae</em> was evaluated in field-caged experiments. The developmental time of immature parasitoids (from egg to adult emergence) and adult female parasitoid longevity were longer than that of males. The average lifetime fecundity per female of <em>O. utetheisae</em> was 56.9 eggs, and the progeny sex ratio was 86.8% female. On average, 2.6 wasps emerged from each <em>R. pedestris</em> egg. The functional response of the parasitoid followed Holling’s Type III model, with a theoretical maximum daily parasitism rate of 14.9 eggs at an optimal host density per day. Parasitism was greater than 90% for 1–8 − day old <em>O. utetheisae</em> females attacking 1–3 − day old <em>R. pedestris</em> eggs. In field − cage trials, parasitism was 47% at 15 (parasitoid): 1 (pest) release ratio. In addition, <em>O. utetheisae</em> females killed 22% <em>R. pedestris</em> eggs by piercing host eggs with their ovipositors. These results indicated that <em>O. utetheisae</em> has significant potential as a biological control agent for augmentative release against <em>R. pedestris</em>.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105829"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different artificial lighting spectra changes the mating behavior of the generalist predator Orius insidiosus (Say), and photoperiod extension promotes its development","authors":"Morgane L. Canovas ,&nbsp;Paul K. Abram ,&nbsp;Roselyne Labbé ,&nbsp;Jean-François Cormier ,&nbsp;Tigran Galstian ,&nbsp;Martine Dorais","doi":"10.1016/j.biocontrol.2025.105831","DOIUrl":"10.1016/j.biocontrol.2025.105831","url":null,"abstract":"<div><div>In protected cropping systems such as greenhouses and indoor farming, augmentative biological control depends on release rates, establishment, and reproduction of natural enemies. Light-emitting diodes (LEDs) are widely used to enhance plant growth in these systems and are increasingly implemented in mass-rearing facilities for natural enemies. However, the impacts of LEDs on the life cycle of beneficial predators remain insufficiently explored. This study examined the mating behaviors and developmental performance of generalist predator <em>Orius insidiosus</em> under light spectra previously shown to support its predation of the pest thrips <em>Frankliniella occidentalis</em>. In laboratory experiments, predator pairs were exposed to artificial light sequences starting with a 12 h baseline solar light condition simulating a cloudy winter day, supplemented by 8 h photoperiod extensions (blue, blue-red, or blue-green–red spectra), or a control without extension. Mating occurred under all tested conditions, but blue light reduced mating frequency and duration. Photoperiod extension improved fecundity, fertility, and resulted in the highest number of second-generation <em>O. insidiosus</em> adults, with blue light favoring egg laying and hatching but not metamorphosis into adults. The second-generation sex ratio was unaffected by light sequence, maintaining population viability with a balanced proportion of females. Our findings demonstrate that <em>O. insidiosus</em> can successfully mate, reproduce, and develop under artificial lighting and highlight the potential of modulating light spectrum to optimize both mass-rearing and establishment in protected crops.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105831"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of tomato treatments with commercial formulations of entomopathogenic fungi on the pest Tuta absoluta and the predator Macrolophus pygmaeus","authors":"Sara Scovero,&nbsp;Silvia T. Moraglio,&nbsp;Luciana Tavella","doi":"10.1016/j.biocontrol.2025.105832","DOIUrl":"10.1016/j.biocontrol.2025.105832","url":null,"abstract":"<div><div><em>Tuta absoluta</em> is a devasting pest causing severe yield losses in most tomato-growing areas. <em>Macrolophus pygmaeus</em> is a generalist predator released in tomato crop to control <em>T. absoluta</em>. Entomopathogenic fungi are also used on tomato against other pests, but they can play a role in controlling <em>T. absoluta</em>. Therefore, the effects of two commercial products Naturalis® and Mycotal®, based on the entomopathogenic fungi <em>Beauveria bassiana</em> and <em>Akanthomyces muscarius</em>, respectively, on <em>T. absoluta</em> and <em>M. pygmaeus</em> were assessed. In no-choice tests on leaflets, mortality was significantly higher on fungus-treated leaflets for 1^st–2^nd instar <em>T. absoluta</em> larvae, and not for 3^rd–4^th instar larvae and for both younger and older <em>M. pygmaeus</em> nymphs. Similarly, in no-choice tests on plants, development rate was significantly lower only for <em>T. absoluta</em> on plants treated with Mycotal®. In two-choice tests, both pest and predator did not show any preference between fungus-treated and untreated plants. Following the laboratory results, Mycotal® was applied also in plastic tunnel cultivation of tomato under field conditions. The number of <em>T. absoluta</em> larvae was significantly lower on plants treated with Mycotal® than on untreated plants, while the presence of <em>M. pygmaeus</em> was not affected by the treatment. Therefore, the use of fungal products together with the predator appears to be a promising strategy for the control of <em>T. absoluta</em> on tomato crop.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105832"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate change impacts on the distribution and ecological niche overlap of Aromia bungii and its natural enemies: implications for biological control","authors":"Liang Zhang,&nbsp;Chaokun Yang,&nbsp;Ping Wang,&nbsp;Guanglin Xie,&nbsp;Wenkai Wang","doi":"10.1016/j.biocontrol.2025.105830","DOIUrl":"10.1016/j.biocontrol.2025.105830","url":null,"abstract":"<div><div>Climate change is profoundly affecting the geographical distribution and ecological adaptability of species, posing new challenges to the spatial distribution patterns of pests and their natural enemies. To assess the potential impact of climate change on the effectiveness of biological control, this study applied ensemble models and ecological niche hypervolume analysis to predict the suitable habitats of <em>Aromia bungii</em> and its natural enemies, <em>Picus canus</em> and <em>Sclerodermus sichuanensis</em>, under current and future climate conditions, while also analyzing the niche overlap between the natural enemies and the pest. The results indicate that under the current climate, the eastern, central, and southern regions of China are key areas where the suitable habitats of natural enemies highly overlap with that of <em>A. bungii</em>. In the future, climate change will expand the suitable habitats of natural enemies and cause the distribution centers of the three species to shift toward higher latitudes. Additionally, the strong overlap of ecological niches of the two natural enemies with <em>A. bungii</em> suggests that they have the potential to control pest populations in terms of ecological adaptations. The findings provide scientific evidence for assessing the spatial dynamics of pests and their natural enemies under the context of climate change, and offer theoretical support for optimizing biological control strategies, formulating precise pest management measures, and maintaining ecosystem stability.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105830"},"PeriodicalIF":3.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High temperatures will likely limit establishment success of the Brazilian peppertree thrips, Pseudophilothrips ichini (Thysanoptera: Phlaeothripidae) in the USA, despite variation in microhabitat thermal environments","authors":"Nathan Harms ,&nbsp;Megann Harlow ,&nbsp;Ian Knight ,&nbsp;Aaron Schad","doi":"10.1016/j.biocontrol.2025.105825","DOIUrl":"10.1016/j.biocontrol.2025.105825","url":null,"abstract":"<div><div>Biological control of the invasive tree, <em>Schinus terebinthifolia</em> Raddi, is under investigation to improve conservation and restoration of native plant communities in the continental USA. Release and monitoring of the Brazilian peppertree thrips, <em>Pseudophilothrips ichini</em>, is ongoing in multiple states and climate zones but establishment has been variable. In particular, establishment has not been confirmed in hot, southern Texas locations despite release of over 150,000 thrips across four locations since 2022. To understand whether thermal limitations may preclude use of <em>P</em>. <em>ichini</em> in some regions, we investigated upper and lower thermal limits of <em>P. ichini.</em> We measured upper limit of chill injury zone (ULCIZ) and lower limit of thermal injury zone (LLTIZ) in the laboratory under multiple combinations of temperature and exposure duration then modeled spatial variation in survival based on detailed weather data over 20 years. Additionally, we documented microhabitat variation in temperature extremes in three release sites in south Texas to evaluate whether thermal refugia exist due to variation in microhabitat conditions. Based on LLTIZ and ULCIZ, thermal injury accumulates rapidly at temperatures above 33.08 ± 0.15 °C and more slowly at decreasing temperatures below 7.85 ± 0.82 °C. Temperatures within the canopy of <em>S. terebinthifolia</em> in the field varied widely and produced a range of predicted mortality, with temperatures at the base of trees that were more moderate in both summer and winter. We determined that, based on the current distribution of <em>S. terebinthifolia</em> in the USA, climate suitability differs between states. Suitability of Florida locations was deemed highly to moderately suitable across seasons, but most Texas locations were unsuitable, including previous release locations in south Texas. These findings suggest releases should continue through most of Florida, in areas of southern California, and locations along the northern gulf coast of Texas. Additionally, augmentative releases of <em>P. ichini</em> during mild seasons may provide opportunities for use of this biological control agent to manage <em>S. terebinthifolia</em> in Texas.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105825"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana","authors":"Qikai Zhang ,&nbsp;Dengjie Wang ,&nbsp;Xinghui Liang ,&nbsp;Haihong Wang ,&nbsp;Baoqian Lyu","doi":"10.1016/j.biocontrol.2025.105824","DOIUrl":"10.1016/j.biocontrol.2025.105824","url":null,"abstract":"<div><div>The leafminer <em>Liriomyza sativae</em> Blanchard (Diptera: Agromyzidae) is a significant pest damaging various agricultural and ornamental crops. Control is hindered by its resistance to multiple insecticides. This study aimed to develop and optimize an autoinoculation device specifically for controlling <em>L. sativae</em> and to assess the device’s efficacy in field applications. The initial phase involved screening to identify a strain of <em>Beauveria bassiana</em> (Balsamo-Crivelli) Vuillemin that was highly virulent to <em>L. sativae</em>. After selection of the optimal strain, the attractant formulation was refined and the device design was optimized to maximize pest attraction and infection rates. Short- and long-term field trials were then conducted to evaluate the device’s effectiveness in the control of pest populations. Results showed that the XJWLMQ-1 strain of <em>B. bassiana</em> had the highest rates of spore production, pest infection, and lethality to the targeted leafminer. The most effective attractant was a blend of <em>trans</em>-2-hexenal, <em>cis</em>-3-hexenol, 1-octen-3-ol, <em>cis</em>-3-hexenyl acetate, and α-ionone in a 4:1:1:3:1 ratio at 0.8 g/L concentration. Optimal efficacy was achieved by positioning the attractant 20 cm from the fungal spores and 20 cm above the cowpea canopy. Both short- and long-term trials confirmed that the autoinoculation device was effective in controlling <em>L. sativae</em> populations, suggesting its effective use for biological control of the target pest.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105824"},"PeriodicalIF":3.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)","authors":"Aline Nunes-Silva ,&nbsp;Camila Costa Moreira ,&nbsp;Janaína Brandão Seibert ,&nbsp;Jonathan Rodríguez ,&nbsp;Italo Delalibera-Júnior","doi":"10.1016/j.biocontrol.2025.105823","DOIUrl":"10.1016/j.biocontrol.2025.105823","url":null,"abstract":"<div><div>Entomopathogenic fungi have been widely used for sustainable agriculture as biological pest control agents. However, commercially available isolates were not efficient in controlling the stink bug <em>Euschistus heros</em> (Hemiptera: Pentatomidae), one of the most destructive species in soybean cultivation. There is an increasing demand for exploring new microorganisms that have the potential to control this insect. The present study evaluated the susceptibility of <em>E. heros</em> to 15 isolates of different species of the genus <em>Metarhizium</em>. A mortality rate of over 95 % was observed in isolates of <em>M. pingshaense</em>, <em>M. humberi,</em> and <em>M. robertsii</em> in 8 days of experiment. The aerial conidia yield of ESALQ 4395 and ESALQ 3364 isolates stood out with a concentration above 1x10⁹ conidia/g of dry rice, which is similar to commercial isolates. The average lethal concentration of these isolates was 5.1x10⁵ conidia/mL for adult insects, with a 95 % confidence interval of 4.7x10⁵ to 5.4x10⁵ conidia/mL for ESALQ 4395 and 4.8x10⁵ to 5.5x10⁵ conidia/mL for ESALQ 3364. However, no age-dependent mortality was observed since mortality among adults was more pronounced than in immature insects. Although low efficiency of entomopathogenic fungi for controlling stink bugs has been associated with the fungistatic and fungicidal action of the volatile compounds present in the alarm pheromone, the data obtained here demonstrated that this insect is highly susceptible to <em>Metarhizium,</em> especially to species that are not explored commercially in Brazil such as <em>M. humberi, M. pinghaense</em> and <em>M. robertsii</em>.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105823"},"PeriodicalIF":3.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass release of susceptible males dilutes Bollgard II® Bt resistance in pink bollworm: A semi-field validated strategy","authors":"Suresh R. Jambagi ,&nbsp;M. Mohan ,&nbsp;K. Muralimohan ,&nbsp;T. Venkatesan ,&nbsp;Satya Nand Sushil","doi":"10.1016/j.biocontrol.2025.105822","DOIUrl":"10.1016/j.biocontrol.2025.105822","url":null,"abstract":"<div><div>The pink bollworm, <em>Pectinophora gossypiella</em> (Lepidoptera: Gelechiidae), poses a significant threat to Bt cotton cultivation in India due to field-level resistance against Cry1Ac and Cry2Ab toxins. Existing pest management tactics have proven inadequate. To address this, homozygous susceptible (RR) male moths were mass-reared in the laboratory and released into Bt Bollgard II® cotton fields. Success was enhanced by trapping and removing feral resistant males with pheromone traps prior to releasing susceptible males. The effectiveness was assessed using bioassays, resistance dilution genetics, fitness cost estimation, field validation, and gene expression analysis. Resistance dilution was evident in both laboratory back-cross progenies and field progenies from feral females mated with released susceptible males. Resistance was autosomal with recessive inheritance for both Cry toxins. In treated plots, boll damage and larval numbers were significantly reduced. Genes linked to Bt resistance, including <em>PgCad1</em>, <em>PgABCA2</em>, <em>APN</em>, and <em>ABCG8</em>, showed significant down regulation (−4.93, −2.63, −1.73, and −1.49 fold, respectively), indicating partial restoration of susceptibility in field-collected larvae following mating (Field-R ♀ × Lab-S ♂). These findings highlight the potential of mass-releasing susceptible males as an effective and sustainable strategy for managing Bt-resistant pink bollworm, providing an alternative to traditional insecticide-based approaches.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105822"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of a novel broad-spectrum antifungal strain Bacillus velezensis Shannan.BV80-12 isolated from the Tibetan Plateau","authors":"Yali Tan ,&nbsp;Feiyan Dong ,&nbsp;Rong Wang ,&nbsp;Xiao Li ,&nbsp;Jialan Zhang ,&nbsp;Tong Gu ,&nbsp;Li Li ,&nbsp;Mengxiang Gao","doi":"10.1016/j.biocontrol.2025.105821","DOIUrl":"10.1016/j.biocontrol.2025.105821","url":null,"abstract":"<div><div>Plant diseases caused by pathogenic fungi are major contributors to global crop loss. Although chemical fungicides are effective for disease control, concerns regarding their adverse effects on human health and the environment have driven interest in sustainable alternatives. Beneficial microorganism-based biological controls have attracted considerable attention in this context. In this study, a novel bacterial strain, <em>Bacillus velezensis</em> Shannan.BV80-12, was isolated from the high-altitude Tibetan Plateau. The strain demonstrated significant inhibitory effects against 21 plant pathogenic fungi across 12 genera, showing inhibition rates between 61.9% and 97.5% in dual-culture assays and between 40.2% and 89.0% in poison agar assays with 2% cell-free supernatant. It also effectively controlled fungal plant diseases in detached leaf assays. Genome sequencing and analysis revealed the genetic basis for its biocontrol efficacy, including 19 biosynthetic gene clusters responsible for secondary metabolites and 23 glycoside hydrolase genes involved in degrading phytopathogen cell walls. UPLC-MS analysis of cell-free supernatants identified six distinct families of antimicrobial metabolites: fengycins (C14–C17), surfactins (C12–C16), iturins (C14–C16), bacillibactin, plantazolicin and bacilysin, suggesting that strain Shannan.BV80-12 possesses the capability to co-produce a variety of bioactive metabolites that synergistically contribute to its strong antimicrobial effects. Genome annotation and comparative genomics further revealed multiple genes associated with bacteria-plant interactions that can enhance plant health and resistance to pathogens, as well as genes crucial for adaptation to harsh environments, which may contribute to the stability and efficacy of the strain in controlling phytopathogens under adverse conditions. These findings elucidate the biocontrol mechanisms of <em>B. velezensis</em> strain Shannan.BV80-12 and support its potential application as a broad-spectrum biocontrol agent against phytopathogenic fungi in sustainable agriculture.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105821"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}