Biological Control最新文献

{"title":"Spatial distribution shifts of Batocera horsfieldi (Coleoptera: Cerambycidae) and its natural enemies in China under climate change: Implications for biological control","authors":"Liang Zhang ,&nbsp;Chaokun Yang ,&nbsp;Ping Wang ,&nbsp;Guanglin Xie ,&nbsp;Wenkai Wang","doi":"10.1016/j.biocontrol.2025.105797","DOIUrl":"10.1016/j.biocontrol.2025.105797","url":null,"abstract":"<div><div>With the increasing impact of climate change on global ecosystems, the ranges of species are undergoing significant changes. The aim of this study was to assess the potential suitable areas for <em>Batocera horsfieldi</em> (Coleoptera: Cerambycidae) and its natural enemies, <em>Dendrocopos major</em> (Piciformes: Picidae) and <em>Scleroderma guani</em> (Hymenoptera: Bethylidae), in the context of climate change and to explore the feasibility of pre-emptive classical biological control strategies. Predictions by ecological niche modelling combined with current and future bioclimatic factors showed that under future climate scenarios, the suitable areas for <em>B. horsfieldi</em> showed a gradual contraction, while the suitable areas for <em>D. major</em> and <em>S. guani</em> were expanding. In addition, by analyzing the overlap of the suitable areas of the three species, we found that in the future, there is a significant spatial overlap in the suitable distribution areas of natural enemies and pests, which provides new possibilities for biological control. This study provides new perspectives for pre-emptive biological control in the future, emphasizes the importance of screening and obtaining approval for releasing natural enemies after the arrival of the pest, especially in the context of the uncertainty caused by climate change, and provides a scientific basis for the development of integrated prevention and management strategies to deal with the threats posed by <em>B. horsfieldi</em> in the future. The results of this study can help to reduce the agro-ecological risks and promote the development of sustainable agriculture.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105797"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A foliar spray made from two Streptomyces spp suppresses Sclerotinia stem rot on canola and inhibits other fungal pathogens","authors":"Louise F. Thatcher ,&nbsp;Cathryn O’Sullivan ,&nbsp;Cindy Myers ,&nbsp;Nick Pain ,&nbsp;Hudson Coates ,&nbsp;Margaret M. Roper","doi":"10.1016/j.biocontrol.2025.105793","DOIUrl":"10.1016/j.biocontrol.2025.105793","url":null,"abstract":"<div><div>An Actinobacteria collection isolated from wheat roots for Fusarium crown rot disease control was screened <em>in vitro</em> and <em>on planta</em> for suppression of fungal pathogens of canola and identified two potent broad-spectrum <em>Streptomyces</em> species strains. Antifungal activity from these strains completely inhibited <em>in vitro</em> growth of <em>Sclerotinia sclerotiorum</em>, both when tested using agar plate competition assays or using culture filtrates derived from liquid cultures. Both strains also inhibited growth of the canola pathogens <em>Leptosphaeria maculans</em> and <em>Rhizoctonia solani</em> in plate assays. Microscopy studies found the <em>Streptomyces</em> sp. strains colonised root and leaf tissues of canola seedlings. In seedling-based studies when applied as a foliar spray, cultures or culture filtrates from both strains prevented development of <em>Sclerotinia</em> leaf lesions. In a mature plant glasshouse pot study, foliar spraying of the cultures or culture filtrates significantly decreased <em>Sclerotinia</em> stem lesion and sclerotia development, with culture filtrates providing a higher level of disease control. In dual sprays applied at two flowering stages, an 87–99 % and 100 % reduction of stem lesions or sclerotia development respectively were recorded on plants sprayed with culture filtrate compared to untreated controls; this was equivalent to disease protection provided by a commercial chemical fungicide control. Grain yields in those treatments increased by 30–49 % relative to controls infected with <em>Sclerotinia</em> without protection. The two <em>Streptomyces</em> sp. strains and their culture filtrates identified in this study have potential for biocontrol applications that maintain or improve crop productivity, reducing the need for chemical disease control in agricultural systems.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105793"},"PeriodicalIF":3.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Versatile role of Bacillus velezensis: Biocontrol of Fusarium poae and wireworms and barley plant growth promotion","authors":"Magdalena Knežević ,&nbsp;Marina Dervišević ,&nbsp;Marina Jovković ,&nbsp;Galina Jevđenović ,&nbsp;Jelena Maksimović ,&nbsp;Aneta Buntić","doi":"10.1016/j.biocontrol.2025.105789","DOIUrl":"10.1016/j.biocontrol.2025.105789","url":null,"abstract":"<div><div>The major losses in cereal production are most commonly caused by phytopathogenic fungi and insect larvae, indicating the need for sustainable pest control management. Although bacterial bioinoculants offer an eco-friendly alternative for plant protection and growth promotion (PGP), their effects on <em>Fusaium poae</em> and <em>Agriotes lineatus</em> larvae have not been comprehensively studied yet. To find an effective biological control agent against these pests, <em>Bacillus</em> strains were isolated from soil and tested for PGP and biocontrol traits, including the presence of antibiotic and toxin-coding genes. Out of eleven strains, <em>B. velezensis</em> BHC 5.6 showed a wide range of PGP and biocontrol abilities, while the presence of <em>fenD, bmyB, srfAA, spaS</em> genes was also detected. Only two strains, <em>P. megaterium</em> BHC 5.5 and B. <em>velezensi</em>s BHC 5.6, showed antifungal effect against <em>F. poae</em> with inhibition percentage of 62% and 67%, respectively. The highest insecticidal effect against wireworms was recorded for <em>B. velezensis</em> BHC 5.6 (56.67%) and <em>B. safensis</em> BHC 11.4 (43.33%). The PGP activity of <em>B. velezensis</em> BHC 5.6 was also confirmed in a pot experiment, where an increment of barley yield was recorded both for infected (17.09%) and uninfected barley seeds (10.12%). This is the first time demonstrating that the <em>B. velezensis</em> BHC 5.6 could be used for integrated pest management of <em>F. poae</em> and <em>A. lineatus</em> larvae in barley and for plant growth promoting. Therefore, the implementation of this strain could contribute to the food safety in sustainable agricultural practices.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105789"},"PeriodicalIF":3.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control and mechanism characterization of endophytic Bacillus velezensis SJ22 against bulb rot of Fritillaria taipaiensis P. Y. Li","authors":"Shijie Wang ,&nbsp;Keke Chen ,&nbsp;Mian Wei ,&nbsp;Le Zhou ,&nbsp;Jiaqi Guo ,&nbsp;Jiaxiang Zhang ,&nbsp;Langjun Cui ,&nbsp;Yi Qiang","doi":"10.1016/j.biocontrol.2025.105792","DOIUrl":"10.1016/j.biocontrol.2025.105792","url":null,"abstract":"<div><div>Bulb rot in <em>Fritillaria taipaiensis</em> P. Y. Li, caused by <em>Fusarium</em> spp., is a destructive soil-borne disease that severely affects the yield and quality of <em>F. taipaiensis</em>. Chemical fungicides are currently the primary means of controlling this disease; however, their use poses risks to human health and the environment. Biological control is thus a preferable alternative to counteract the threats associated with pathogens. In this study, an endophytic bacterium, designated SJ22, was isolated from bulb tissue of <em>F. taipaiensis</em>, and it exhibited significant antagonism against <em>Fusarium</em> spp., with inhibition rates on plates ranging from 84.35% to 89.96% and a control rate of 73.38% in pot experiments. Morphological observations and phylogenetic analyses identified the isolate SJ22 as <em>Bacillus velezensis</em>. Furthermore, the antifungal mechanisms of SJ22 include the inhibition of spore germination, alteration of hyphal morphology, and disruption of cell membrane integrity. Its volatile organic compounds exhibited broad-spectrum antifungal activity <em>in vitro</em>, with an inhibition rate of 28.36% to 59.12%, and demonstrated significant control efficacy <em>in vivo</em>. Transcriptome analysis revealed that SJ22 induced systemic resistance in <em>F. taipaiensis</em> by upregulating defense-related genes, primarily through enhancing the phenylpropanoid biosynthesis pathway and promoting the salicylic acid/ethylene signaling pathway to induce the production of pathogenesis-related proteins and increase the activity of defense-related enzymes. In summary, we isolated an endophytic bacterium that exerts its biocontrol function through both inhibiting pathogen growth and inducing resistance.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105792"},"PeriodicalIF":3.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of biological control on pest survival and Aspergillus flavus in two stored maize genotypes","authors":"J. Parsons ,&nbsp;M.S. Lopes ,&nbsp;Monica Mezzalama ,&nbsp;J. Riudavets","doi":"10.1016/j.biocontrol.2025.105791","DOIUrl":"10.1016/j.biocontrol.2025.105791","url":null,"abstract":"<div><div>During storage, maize is attacked by various insect pests that damage its seeds and reduce their quality. A promising approach to control these pests is the use of natural enemies. However, there is limited knowledge about how natural enemies interact with different maize genotypes or generations. Additionally, the presence of storage pests often creates favorable conditions for <em>Aspergillus flavus</em>, one of the most common storage fungi. This study evaluates the biological control of three common storage pests: <em>Sitotroga cerealella, Sitophilus zeamais,</em> and <em>Oryzaephilus surinamensis</em>, by their respective natural enemies: <em>Blattisocius tarsalis, Anisopteromalus calandrae,</em> and <em>Cephalonomia tarsalis.</em> It examines how these biocontrol agents impact pest survival, kernel damage and <em>A. flavus</em> conidia count. These traits are compared across two commercial maize genotypes: a transgenic genotype expressing the insecticidal Cry1Ab protein and its non-transgenic near-isoline counterpart. Commercial seeds are used for both genotypes (F1), along with seeds obtained from field-grown transgenic and near-isoline plants (F2). Our findings demonstrated that natural enemies effectively reduced pest populations and pest-induced kernel damage, particularly in the F2 transgenic genotypes. The presence of <em>A. flavus</em> increased with kernel damage and rising pest populations; however, natural enemies suppressed fungal activity by reducing pest numbers, thereby improving kernel quality. Overall, these findings support the potential of an integrated pest management strategy combining transgenic resistance with biological control to manage pests and <em>A. flavus</em>, thus enhancing maize quality and safety during storage.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105791"},"PeriodicalIF":3.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana","authors":"Qian Nong ,&nbsp;Yan Zhang ,&nbsp;Yanyan Long ,&nbsp;Yanlu Chen ,&nbsp;Liping Qin ,&nbsp;Shanyu Lin ,&nbsp;Fenghua Zeng ,&nbsp;Ling Xie","doi":"10.1016/j.biocontrol.2025.105781","DOIUrl":"10.1016/j.biocontrol.2025.105781","url":null,"abstract":"<div><div>Banana wilt-like disease, triggered by <em>Fusarium oxysporum</em>, poses a severe threat to banana cultivation as a persistent soilborne pathogen. A strategy to effectively control this disease has yet to be found. Dark septate endophytes (DSEs) fungi have emerged as promising biocontrol agents, not only offering protection against plant pathogens but also enhancing plant growth. Herein, we isolated a novel DSEs species, <em>Acidomelania saccharicola</em> LZ3, from sugarcane (<em>Saccharum officinarum</em>) soil in Liuzhou, Guangxi Province, China. Its classification as a new species was confirmed through morphological characterization and DNA sequence analysis. <em>A. saccharicola</em> LZ3 promoted the growth of banana and inhibited the growth of <em>F. oxysporum</em> by 73.28% in a Petri dish assay, and reduced disease by 52.82% in pots. The average incidence rate of Banana-LZ3 symbionts transplanted into a field highly contaminated with <em>F. oxysporum</em> was 25.93% and the control effect was 53.27%. Inoculation of banana plants with <em>A. saccharicola</em> LZ3 significantly increased polyphenol oxidase (POD) and superoxide dismutase (SOD) activity. This research lays the groundwork for future investigations into <em>A. saccharicola</em> and other DSEs, exploring their potential in sustainable plant disease management.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105781"},"PeriodicalIF":3.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris","authors":"Moon Joo Kim ,&nbsp;Eun-Jae Park ,&nbsp;Bori Lee ,&nbsp;Hyun-Jae Jang ,&nbsp;Jongmin Ahn ,&nbsp;Ji-Hye Kim ,&nbsp;Young Ho Kim ,&nbsp;Bong-Sik Yun ,&nbsp;Seung Woong Lee","doi":"10.1016/j.biocontrol.2025.105775","DOIUrl":"10.1016/j.biocontrol.2025.105775","url":null,"abstract":"<div><div><em>Xanthomonas campestris</em> pv. <em>campestris</em> (Xcc) is a gram-negative bacterium that causes black rot, a disease that significantly reduces cruciferous crops yields. Crop production is facing environmental and health challenges associated with the use of synthetic chemicals, highlighting the need for effective biological control agents as sustainable alternatives. In this study, we isolated and characterized the <em>Bacillus velezensis</em> 21–128 strain for antimicrobial activity against Xcc using pot experiments and various enzymatic activity assays. We used ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis to confirm the presence of lipopeptides, including surfactins in the ethyl acetate extract from <em>Bacillus velezensis</em> 21–128. Four types of surfactins—C13, C14, C15, and C16—were identified by mass fragmentation analysis. Further, we used a combination of separation methods, including HP-20 column chromatography, MPLC, and semi-preparative HPLC to isolate and purify the identified surfactins. The purified surfactins were compared with standard substances using HPLC and identified through comparative analysis with LC-MS data. Lastly, we evaluated the antimicrobial activity of the isolated surfactins against the Xcc strain, and determined the minimum inhibitory concentration values for the total surfactin group, G2, G3, G4, and G5. These results suggest that the <em>Bacillus velezensis</em> strain 21–128 could serve as a biological control agent against Xcc and could be a promising strategy against black rot in cruciferous crops.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105775"},"PeriodicalIF":3.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol activity of novel and known bioproducts based on Bacillus strains and basic products against Botrytis cinerea in tomato fruit","authors":"Salvina Panebianco,&nbsp;Silvia Americo,&nbsp;Angela Roberta Lo Piero,&nbsp;Gabriella Cirvilleri","doi":"10.1016/j.biocontrol.2025.105780","DOIUrl":"10.1016/j.biocontrol.2025.105780","url":null,"abstract":"<div><div>The use of bioproducts based on native microorganisms improves pre- and postharvest disease control by enhancing adaptability during storage and colonizing ability of fruit surface. This study aims to evaluate the effectiveness of three tomato-isolated <em>Bacillus</em> strains (<em>B. cereus</em> 6C, <em>B. thuringiensis</em> 18D and <em>B. velezensis</em> 23A) and of three commercial bioproducts based on <em>B. amyloliquefaciens</em> subsp. <em>plantarum</em> D747, chitosan hydrochloride and <em>Equisetum arvense</em>, alone and in mixture, in controlling <em>B. cinerea</em> on tomato fruit, investigating the possible mechanisms of action involved in the biocontrol activity of bacterial strains. All the tested strains reduced disease incidence and severity by more than 60 % when applied 3 days before pathogen inoculation, in both wound- and dip-treatments, showing performance similar or better than commercial bioproducts. <em>B. velezensis</em> 23A showed the best performance and persistence through time, even when applied 10 days before pathogen challenging. Monitoring of <em>Bacillus</em> population dynamics during tomato storage revealed good survival of the antagonists. <em>Bacillus</em> strains were able to secrete toxic metabolites and enzymes responsible for cell wall degradation. Conversely, the expression of tomato genes involved in salicylic acid (SA) and jasmonic acid (JA) signaling, as well as in pathogenesis related proteins (PRs) biosynthesis, was not induced by <em>B. velezensis</em> 23A. Taken together, the results highlighted that the ability of bacterial strains to colonize the host surface for long periods and to secrete toxic metabolites and enzymes is probably the main factor on which the level of disease control depends. Moreover, this study provides the basis for future developing carposphere-competent bio-inoculants to be used in preharvest for an ecofriendly management of postharvest grey mold.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"206 ","pages":"Article 105780"},"PeriodicalIF":3.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genome and transcriptome analysis of the endophytic fungus Epichloë sibirica reveals biological control mechanism in host Achnatherum sibiricum","authors":"Xinjian Shi ,&nbsp;Tianzi Qin ,&nbsp;Yubao Gao ,&nbsp;Nianxi Zhao ,&nbsp;Anzhi Ren","doi":"10.1016/j.biocontrol.2025.105778","DOIUrl":"10.1016/j.biocontrol.2025.105778","url":null,"abstract":"<div><div>Endophytic fungi of the genus <em>Epichloë</em> can bolster the host’s immune defenses against phytopathogens. However, the underlying mechanisms by which the endophytes regulate host responses remain poorly understood. This study elucidates the tripartite strategy employed by <em>Epichloë sibirica</em> to protect <em>Achnatherum sibiricum</em> through comparative genomics and transcriptomics. First, genomic and transcriptomic analyses revealed that <em>E. sibirica</em> can synthesize antibiotics (e.g., ε-poly-L-lysine) and mycelial degrading enzymes. Second, ecological niche occupation was supported by (1) minimal transcriptomic alterations in <em>E. sibirica</em> during pathogen challenge, (2) superior antimicrobial activity in fungal fermentation assays (superior antimicrobial activity of <em>E. sibirica</em> against four pathogens, while pathogen-derived broths had negligible effects on <em>E. sibirica</em> growth), and (3) comparative antibiotic susceptibility testing demonstrated <em>E. sibirica</em>’s significantly higher tolerance to strobilurin A, tetracycline, streptomycin sulphate, and penicillin sodium compared to pathogens. Third, host resistance induction involved gene clusters encoding effector proteins, cell wall-modifying enzymes, and trehalose. These findings identify <em>E. sibirica</em> as a multifaceted biocontrol agent through metabolite-mediated antagonism, ecological niche occupation, and host priming. Our results provide omics-level evidence of endophyte-driven tripartite defense mechanisms.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105778"},"PeriodicalIF":3.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Action of Pythium oligandrum on Grapevine Trunk Diseases and its impact on microbial communities","authors":"Séverine Lopez ,&nbsp;Alexandre Chataigner ,&nbsp;Jessica Vallance ,&nbsp;Ahmed Taïbi ,&nbsp;Assia Dreux-Zigha ,&nbsp;Marie-Cécile Dufour","doi":"10.1016/j.biocontrol.2025.105779","DOIUrl":"10.1016/j.biocontrol.2025.105779","url":null,"abstract":"<div><div>Grapevine Trunk Diseases (GTDs) have become a major challenge for viticulture. Since the ban of sodium arsenate (the only approved pesticide) in 2001, the need for alternative methods, such as biocontrol, has become increasingly urgent. One promising microorganism is the oomycete <em>Pythium oligandrum,</em> which is known to improve plant health by increasing natural defences and reducing disease incidence by up to 40 %. In order to use this microorganism in vineyards, it need first to be formulated and tested to ensure its safety. An experiment was therefore carried out in a greenhouse under semi-controlled conditions to assess the efficacy of the <em>P. oligandrum</em> biopesticide produced and its impact on microbial communities using a high-throughput sequencing approach. Vines were treated with the biopesticide and were inoculated with two fungi involved in wood diseases: <em>Neofusicoccum parvum</em> (involved in <em>Botryosphaeria</em> dieback) and <em>Phaeomoniella chlamydospora</em> (involved in Esca). During a three-month experiment, samples of the rhizosphere environment were collected to assess possible changes in microbial communities, either as part of GTDs or as a result of the action of <em>P. oligandrum</em>. The results indicated a minimal effect of the <em>P. oligandrum</em> biopesticide on the total microbial community of the vine rhizosphere. However, the treatment appeared to enhance several beneficial taxa that play a role as plant growth promoting rhizobacteria (PGPR) or biocontrol agents (BCA). This improvement, together with the direct effect of <em>P. oligandrum</em>, may explain the reduction in necroses caused by <em>N. parvum</em> and <em>P. chlamydospora</em> following the biopesticide application.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"205 ","pages":"Article 105779"},"PeriodicalIF":3.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}