Biological Control最新文献

{"title":"Biocontrol of Chrysanthemum wilt by Bacillus velezensis HSX-1 and its antifungal mechanism","authors":"Jian Wang,&nbsp;Yaping Wang,&nbsp;Wenchao Yu,&nbsp;Jiayun Chen,&nbsp;Yangang Dai,&nbsp;Zikang Li,&nbsp;Hang Wu,&nbsp;Xiaoqian Hu,&nbsp;Shufeng Bi","doi":"10.1016/j.biocontrol.2026.105993","DOIUrl":"10.1016/j.biocontrol.2026.105993","url":null,"abstract":"<div><div>Chrysanthemum wilt caused by <em>Fusarium oxysporum</em> is a devastating disease that causes substantial economic losses. Biological control agents are considered an effective approach to control plant diseases. In the present study, the biocontrol efficacy and mechanisms of the newly isolated <em>Bacillus velezensis</em> strain HSX-1 against <em>F. oxysporum</em> were evaluated. <em>B. velezensis</em> HSX-1 significantly suppressed the mycelial growth of <em>F. oxysporum</em> by 65.52% in the dual culture test. In addition, a 15% concentration of the cell-free supernatant (CFS) from <em>B. velezensis</em> HSX-1 significantly suppressed colony radial growth as well as spore germination of <em>F. oxysporum</em>. Whole-genome sequencing identified gene clusters responsible for biocontrol activity, including those encoding nonribosomal peptides, polyketide antibiotics, siderophores, and extracellular hydrolases. <em>B. velezensis</em> HSX-1 has the ability to effectively reduce the virulence of <em>F. oxysporum</em> and promoted the growth of <em>Chrysanthemum morifolium</em>. Strain HSX-1 produces multiple plant growth-promoting metabolites, including indole-3-acetic acid (IAA) and siderophores. Phenotypic and transcriptome analyses revealed that strain HSX-1 inhibits <em>F. oxysporum</em> by disrupting the cell membrane integrity and reactive oxygen species (ROS) scavenging system. All of these results indicated that <em>B. velezensis</em> HSX-1 has strong potential to be developed as a biocontrol agent to control Chrysanthemum wilt caused by <em>F. oxysporum</em> and promote <em>C. morifolium</em> growth.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105993"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus velezensis BN suppresses Fusarium tricinctum-induced lily wilt disease through multiple biocontrol mechanisms","authors":"Fan Wang ,&nbsp;Xuetao Wang ,&nbsp;Xu Wang ,&nbsp;Litao Wang ,&nbsp;Chenying Wu ,&nbsp;Yang Liu ,&nbsp;Jiaxin Zheng ,&nbsp;Xinyi Yang ,&nbsp;Constantine Uwaremwe ,&nbsp;Zhaoyu Li ,&nbsp;Zheng Zhang ,&nbsp;Yonghong Zhu ,&nbsp;Yongqiang Tian","doi":"10.1016/j.biocontrol.2026.105978","DOIUrl":"10.1016/j.biocontrol.2026.105978","url":null,"abstract":"<div><div>Lily wilt caused by <em>Fusarium tricinctum</em> severely constrains the production of lily (<em>Lilium davidii</em> var. <em>unicolor</em>), and effective, sustainable control strategies are urgently needed. In this study, <em>Bacillus velezensis</em> strain BN was shown to suppress lily wilt disease through multiple biocontrol mechanisms. In vitro, cell-free filtrates of BN exhibited strong antifungal activity by significantly inhibiting mycelial growth and spore germination, with conidial germination inhibition reaching 87.6% at a filtrate concentration of 50% (v/v), while also inducing hyphal swelling and lysis and disrupting membrane integrity. Under greenhouse conditions, preventive application of BN provided more effective disease suppression than curative treatment. GFP labeling confirmed that BN could stably colonize lily roots, supporting its persistence in the rhizosphere. In addition, BN treatment significantly enhanced host defense responses, as evidenced by increased activities of catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). Metagenomic analysis further revealed that BN application reshaped the rhizosphere microbial community, enriching beneficial bacterial taxa and functional pathways related to carbon metabolism, secondary metabolite biosynthesis, and plant–microbe interactions. Together, these results indicate <em>B. velezensis</em> BN functions as a multifaceted biocontrol agent by simultaneously antagonizing the pathogen, enhancing host immune responses, and modulating a disease-suppressive rhizosphere microbiome, highlighting its potential for sustainable management of lily wilt disease.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105978"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frass adjuvants improve the efficacy of Steinernema feltiae against vine mealybug, Planococcus ficus (Hemiptera: Pseudococcidae) under laboratory conditions","authors":"Alexander M. Butcher ,&nbsp;Lan Xue ,&nbsp;Xinyu Li ,&nbsp;Vaughn M. Walton","doi":"10.1016/j.biocontrol.2026.105985","DOIUrl":"10.1016/j.biocontrol.2026.105985","url":null,"abstract":"<div><div>Vine mealybug, <em>Planococcus ficus</em>, is a major vineyard pest, complicating vineyard management due to its cryptic behavior and patchy distributions. This insect is also a major vector of the highly damaging grapevine leafroll-associated viruses. Entomopathogenic nematodes e.g., <em>Steinernema feltiae</em> shows promise for biological control, its efficacy is limited by desiccation under field conditions. This study evaluated insect-derived frass and chitin as sustainable adjuvants to improve nematode survival and performance. Frass collected from <em>Hermetia illucens</em> larvae reared on three feedstocks of varying fiber content, as well as isolated pupal exuviae, was assessed for water-holding capacity, effects on nematode activity, and impacts on mealybug mortality. High- and intermediate-fiber frass retained significantly more water than exuviae and extended nematode activity by up to three days. When paired with high-fiber frass, nematode applications caused 94% mealybug mortality compared to 76% with nematodes alone. Logistic regression further revealed a 125-fold increase in adult mortality when treated with nematodes plus frass relative to controls. Frass-based adjuvants also increased nematode penetration and load in adult females by up to 3.5-fold. These findings highlight the dual physical and biological benefits of frass, supporting its potential as a cost-effective and scalable adjuvant for insect pest control, including mealybug biocontrol.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105985"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable control of Phytophthora capsici in tomato (Solanum lycopersicum L.) using garden waste compost tea enriched with Streptomyces AC-3","authors":"M.Belén Suárez-Fernández ,&nbsp;Mario González-García ,&nbsp;Ana I. González-Hernández ,&nbsp;M.Ángeles Gómez-Sánchez ,&nbsp;Beatriz Andreo-Jiménez ,&nbsp;M.Remedios Morales-Corts","doi":"10.1016/j.biocontrol.2026.105989","DOIUrl":"10.1016/j.biocontrol.2026.105989","url":null,"abstract":"<div><div>The search for sustainable alternatives to chemical pesticides remains a key priority in crop protection. This study evaluates the suppressive potential of a compost tea (CT) derived from pruning and garden residues, together with a native <em>Streptomyces</em> isolate (AC-3) with strong in vitro antagonism against <em>Phytophthora</em> spp. Two CT formulations with compost-to-water ratios of 1:5 and 1:3.5 (v/v) were evaluated to assess whether a more concentrated formulation could maintain chemical and microbiological quality while facilitating product handling and commercialization, and whether its application, alone or in combination with <em>Streptomyces</em> AC-3, could suppress pathogen growth and reduce disease development in plants. The concentrated CT (1:3.5) exhibited higher electrical conductivity, nutrient content, and microbial abundance without major changes in the overall microbial structure, as confirmed by metagenomic analysis. Dominant bacterial genera included <em>Mesorhizobium, Micromonospora,</em> and <em>Streptomyces</em>, taxa associated with nutrient cycling and biocontrol. <em>Streptomyces</em> AC-3 showed strong inhibition of oomycetes, including <em>P. capsici</em>, while not affecting beneficial fungi such as <em>T. harzianum</em>. Greenhouse assays demonstrated that CT enhanced root biomass and significantly reduced disease incidence in tomato plants, with the combined treatment (CT plus AC-3) achieving the highest protection. These results highlight the potential of CT as both a biostimulant source and a reservoir of beneficial microorganisms. The combination of CT with selected <em>Streptomyces</em> strains represents a promising, eco-friendly strategy for the management of oomycete pathogens within sustainable agricultural systems.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105989"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression of pine pitch canker disease and promotion of seedling growth in Japanese red pine using a newly isolated strain of Bacillus subtilis","authors":"Su-Yeon Lee ,&nbsp;Sang-Jae Won ,&nbsp;Jae-Hyun Moon ,&nbsp;Vantha Choub ,&nbsp;Su-In Choi ,&nbsp;Ju-Yeol Yun ,&nbsp;Henry B. Ajuna ,&nbsp;Young Sang Ahn","doi":"10.1016/j.biocontrol.2026.105990","DOIUrl":"10.1016/j.biocontrol.2026.105990","url":null,"abstract":"<div><div><em>Fusarium circinatum</em>, the causal agent of pine pitch canker, can cause large-scale tree mortality in pine forests. Asymptomatic seedlings infected with <em>F. circinatum</em> can spread the disease at planting sites, highlighting the need for nursery-level control and using vigorous seedlings. Accordingly, this study isolated the plant growth-promoting bacterium (PGPB) <em>Bacillus subtilis</em> SJ 910 and evaluated its efficacy in controlling <em>F. circinatum</em> and enhancing pine-seedling growth. <em>B. subtilis</em> SJ 910 produced lytic enzymes (chitinase, protease, and β-1,3-glucanase) associated with the degradation of major fungal cell wall components. Crude enzyme extracts inhibited <em>F. circinatum</em> spore germination by 72.20%, 79.06%, and 87.36% and mycelial growth by 7.08%, 15.93%, and 21.24% at concentrations of 25, 50, and 100 μL/mL, respectively, and induced abnormal morphologies in spores and hyphae. Application of <em>B. subtilis</em> SJ 910 significantly suppressed pitch canker, reducing disease incidence to less than half and markedly lowered disease severity compared with the control and pink broth (PB) media treatments, while also increasing seedling survival. Moreover, <em>B. subtilis</em> SJ 910 produced indole-3-acetic acid and solubilized nitrogen and phosphorus, contributing to enhanced seedling growth. <em>B. subtilis</em> SJ 910 markedly enhanced seedling biomass, substantially increasing both shoot and root dry weights compared with control and PB media treatments. These findings suggest that <em>B. subtilis</em> SJ 910 is a promising biocontrol agent against <em>F. circinatum</em> and a growth promoter for pine seedlings, offering a sustainable alternative to chemical treatments and contributing to the production of vigorous seedlings for successful afforestation.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105990"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root-irrigation with Bacillus velezensis TX2 induces systemic resistance to soft rot disease via jasmonic acid and salicylic acid dependent pathways in Colocasia esculenta","authors":"Yi Chen ,&nbsp;Yujian He ,&nbsp;Shitou Xia","doi":"10.1016/j.biocontrol.2026.105976","DOIUrl":"10.1016/j.biocontrol.2026.105976","url":null,"abstract":"<div><div><em>Pectobacterium carotovorum</em> (<em>Pcc</em>) is the causative organism of bacterial soft rot disease, one of the most hazardous diseases affecting <em>Colocasia esculenta</em> (L.) Schott. Currently, chemical fungicides are still useful in controlling soft rot disease, but their constant usage not only makes bacteria more resistant but also poses risks to the ecosystem. Plant growth-promoting rhizobacteria (PGPRs), however, provide an alternative for disease control and sustainable development. In this investigation, we obtained an isolate of <em>Bacillus velezensis</em> (TX2) from the soil of the taro rhizosphere. In <em>C. esculenta</em>, TX2 root-irrigation was then shown to stimulate auxin production and plant growth. More interestingly, it was discovered that taro developed resistance to <em>Pcc</em> following this TX2 root-irrigation treatment. Through whole genome sequencing, we found 13 homologous genes related to biosynthesis of plant growth-promoting and resistant secondary metabolite. Furthermore, TX2 application was confirmed to enhance the expression of resistant marker genes <em>PDF1.2, PR1</em> and <em>PR2</em> by transcriptomic analysis as well as qRT-PCR verification. The concentrations of JA, JA-Ile, and SA were also raised significantly in taro plants with the application of TX2. Collectively, our results showed that TX2 enhances taro plant resistance to soft rot by strengthening the SA and JA pathways and may serve as a promising biocontrol agent.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105976"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial-based biocontrol of Fusarium species causing wheat crown rot: current status and future prospects","authors":"Aisha Lawan Idris ,&nbsp;Yuan Xie ,&nbsp;Deyao Kong ,&nbsp;Qingqing Li ,&nbsp;Surayya Lawan Idris ,&nbsp;Khadija Batool ,&nbsp;Fei Gao ,&nbsp;Xiaoting Zhang","doi":"10.1016/j.biocontrol.2026.105977","DOIUrl":"10.1016/j.biocontrol.2026.105977","url":null,"abstract":"<div><div><em>Fusarium</em> species are major pathogens that significantly reduce wheat productivity. It is quite understood that chemical fungicides are efficient in disease management, however their adverse effects that continue to pose threat to human health and the environment necessitates the need for alternative strategies. In order to solve this problem, scientists have conducted researches and explored environmentally friendly approaches including microbial control to curtail the adverse effects of <em>Fusarium</em> spp. causing wheat crown rot (WCR) disease. Based on existing researches conducted in recent time and the past few years, the current study showed that microbial control agents including bacteria of the genera <em>Bacillus</em>, <em>Pseudomonas</em> among others play key role in the biocontrol of WCR disease. At the same time, fungal and viral control agents such as <em>Trichoderma</em> and megabirnavirus showed promising control effects against <em>Fusarium</em> spp. These biocontrol agents utilize competition for nutrient or space, synthesized secondary metabolites, volatile organic compounds, induced systemic resistance, produce enzymes or alter genes expression. These prevent early infection, degrades <em>Fusarium</em> spp. cell wall, and inhibits mycelial growth which further reduced WCR disease severity. In this study, we highlighted some hypotheses and suggests future perspectives for subsequent studies in order to ensure sustainable field application of microbial control agents (MCAs).</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105977"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of entomopathogenic nematodes and entomopathogenic fungi against the soil-dwelling stages of spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae)","authors":"Rambika Thapa ,&nbsp;Muhammad Usman ,&nbsp;Emile Cole ,&nbsp;Rufus Isaacs ,&nbsp;Jaime C. Piñero ,&nbsp;David Shapiro-Ilan ,&nbsp;Yoensuk Chung ,&nbsp;Marisol Quintanilla","doi":"10.1016/j.biocontrol.2026.105986","DOIUrl":"10.1016/j.biocontrol.2026.105986","url":null,"abstract":"<div><div>Spotted-wing drosophila (SWD), <em>Drosophila suzukii</em>, is an invasive pest of small fruits such as blueberries and raspberries, causing substantial yield losses. This study evaluated the biocontrol potential of entomopathogenic nematodes (EPNs) and entomopathogenic fungi (EPF) against the last instar larvae and pupae of <em>D. suzukii</em>. In the first experiment, three EPN species, <em>Steinernema carpocapsae</em> (ALL strain), <em>S. feltiae</em> (SN strain), and <em>Heterorhabditis bacteriophora</em> (SN strain), were applied at a rate of 50 infective juveniles (IJs) cm⁻². <em>S. feltiae</em> was the most effective, reducing adult <em>D. suzukii</em> emergence to 20 % from larvae and 40 % from pupae. A subsequent experiment tested nine EPN species, including <em>S. carpocapsae</em>, <em>S. riobrave</em> (355 strain), <em>S. glaseri</em> (VS strain), <em>H. indica</em> (HOM1 strain), <em>H. georgiana</em>, <em>H. floridensis</em>, and <em>H. megidis</em> (UK211 strain). Among these, <em>S. feltiae</em>, <em>S. carpocapsae</em>, and <em>S. glaseri</em> consistently reduced adult emergence to below 50 %, with <em>S. feltiae</em> achieving 20 % emergence at 100 IJs cm⁻². In a third experiment, three EPF, <em>Beauveria bassiana</em>, <em>Metarhizium brunneum,</em> and <em>Cordyceps fumosorosae</em>, were evaluated. <em>B. bassiana</em> proved most effective, reducing <em>D. suzukii</em> adult emergence by more than 50 % at 1 × 10⁸ conidia mL⁻¹. In the final experiment, combinations of <em>S. carpocapsae</em>, <em>S. feltiae</em>, or <em>S. glaseri</em> with <em>B. bassiana</em> at both 50 and 100 IJs cm⁻² further reduced emergence, with the most effective treatment resulting in only 8 % adult emergence. These findings demonstrate the strong potential of EPNs and EPF, individually and in combination, as biocontrol agents targeting the soil-dwelling stages of <em>D. suzukii</em>.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105986"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Pseudomonas frederiksbergensis FC-17 on potato bacterial wilt and rhizosphere microbial community","authors":"Sheng Li ,&nbsp;Ximiao Xu ,&nbsp;Yixian Chen ,&nbsp;Xia Wang ,&nbsp;Xiang Li ,&nbsp;Jieyu Li ,&nbsp;Meiru Zhu ,&nbsp;Lang Yan ,&nbsp;Haiyan Wang","doi":"10.1016/j.biocontrol.2026.105988","DOIUrl":"10.1016/j.biocontrol.2026.105988","url":null,"abstract":"<div><div><em>Pseudomonas frederiksbergensis</em> FC-17 shows prominent biological control for potato wilt disease caused by <em>Ralstonia solanacearum</em>. It remains unknown whether the biocontrol strain can effectively colonize rhizosphere soil and how it affects the bacterial community. Therefore, we used qPCR to track the abundance changes of FC-17 in the potato rhizosphere within 30 days after pathogen inoculation. The findings showed that applying bio-organic fertilizer, produced by secondary solid fermentation of organic fertilizer and FC-17, effectively improved disease control but did not enhance FC-17 colonization. 16S rRNA gene amplicon sequencing indicated that disease suppression was primarily attributed to the alterations in bacterial community composition, rather than the competitiveness of the inoculated FC-17 strain. Under pathogen pressure, bio-organic fertilizer treatment enriched specific bacterial genera, including <em>Sphingomonas</em>, <em>Sphingobium</em>, <em>Lysobacter</em>, and <em>Isoptericola</em>. Among these, <em>Lysobacter</em> and <em>Sphingomonas</em> were negatively correlated with the disease index, suggesting their potential role in suppressing potato bacterial wilt. This study elucidates a microbiome-mediated biocontrol mechanism, highlighting that optimizing the resident microbial community is a crucial strategy for sustainable disease management. Our results provide a theoretical foundation for combining <em>P. frederiksbergensis</em> with organic fertilizer to control potato bacterial wilt.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105988"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and diagnostic advances for basal stem rot management in oil palm","authors":"Qudrat Ullah ,&nbsp;Waqas Haider ,&nbsp;Muhammad Waqar ,&nbsp;Farhang Hameed Awlqadr ,&nbsp;M.Y. Nur Athiqah ,&nbsp;Iqra Rafiq ,&nbsp;Thanet Khomphet ,&nbsp;Shams Shaila Islam ,&nbsp;Temesgen Anjulo Ageru","doi":"10.1016/j.biocontrol.2026.105992","DOIUrl":"10.1016/j.biocontrol.2026.105992","url":null,"abstract":"<div><div>Basal stem rot (BSR), caused by <em>Ganoderma boninense</em>, is the most destructive disease affecting oil palm (<em>Elaeis guineensis</em>), leading to yield losses of up to 70% in severely affected plantations. This review synthesizes recent advances in understanding the physiological and molecular mechanisms underlying BSR, including pathogen genetic diversity, effector protein functions, and host-pathogen interaction. Emphasis is placed on AI-based diagnostics such as RNA <em>aptasensors</em> and UAV-assisted remote sensing, which enable early, high-precision disease detection. The review also discusses CRISPR-mediated resistance breeding and the application of biological control agents, particularly <em>Trichoderma</em> spp. and <em>endophytic</em> fungi. By integrating genomic insights, molecular detection tools, and resistance breeding, a targeted framework integrating molecular diagnostics and genetic resistance is proposed for effective BSR management, improving oil palm resilience while minimizing environmental and economic impacts. In this review, a multi-omics model is suggested, combining the genomics of Ganoderma boninense pathogenicity with AI diagnostics and CRISPR resistance breeding to facilitate precise early intervention, bridge the scalability gap, strain variability, and the applicability of smallholder to BSR sustainable management. The connectivity of these advances achieves the bridge of the key gaps in scalability of the field, strain variability, and smallholder accessibility enabling the development of a sustainable disease control in oil palm.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105992"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}