Biological Control最新文献

{"title":"Preemptive and proactive application of biological control for weeds: An argument for swifter action to aid conservation efforts","authors":"Melissa C. Smith ,&nbsp;Kim Canavan ,&nbsp;Carey R. Minteer ,&nbsp;Deah Lieurance","doi":"10.1016/j.biocontrol.2025.105725","DOIUrl":"10.1016/j.biocontrol.2025.105725","url":null,"abstract":"<div><div>Invasive alien plants (IAPs) are a frequent consequence of global connectivity and present significant threats to biodiversity, amplifying impacts from global climate change and habitat loss. Integrated management efforts for landscape-level plant invasions often include some combination of mechanical, cultural, chemical, and biological control. The former three have well established protocols and development pipelines for rapid responses to new invasions. Biological control of IAPs, however, is often employed only after the invaded region has reached some arbitrary but intolerable level of negative impact that triggers efforts to develop agents to provide control. Despite mounting evidence that investments in prevention and proactive approaches to IAPs are the most cost effective, most expenditures, including those for biological control development, continue to be in the post-invasion reactive phase. We build a rationale for earlier investigation and implementation of biological control for IAPs. A potential framework for this approach would pair prioritization methods (e.g., risk assessments and horizon scanning) to identify targets with extensive literature searches for known herbivores or foreign range surveys and early host range tests. In addition, resource sharing among regions and nations with similar climates and risks would alleviate the onus of investment from any one party. Finally, investments into conservation and training opportunities between nations further incentivizes maintaining natural resources for potential biological control. By developing and implementing biological control earlier in or before the invasion process, countless impacts and costs are lessened.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"202 ","pages":"Article 105725"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatiles produced by bacteria in the honeydew of cotton aphids mediate prey location by Hippodamia variegata (Coleoptera: Coccinellidae)","authors":"Panjing Liu ,&nbsp;Tao Zhang ,&nbsp;Yu Gao ,&nbsp;Xiaofang Zhang ,&nbsp;Hongyi Wei ,&nbsp;Yanhui Lu","doi":"10.1016/j.biocontrol.2025.105728","DOIUrl":"10.1016/j.biocontrol.2025.105728","url":null,"abstract":"<div><div>Honeydew produced by insects is not only recognized as a nutrient-rich substance for natural enemies, influencing their survival, development, and reproduction but also a source of volatiles that regulate the foraging behavior of natural enemies. <em>Hippodamia variegata</em> (Coleoptera: Coccinellidae) is an effective predatory natural enemy of <em>Aphis gossypii</em> (Hemiptera: Aphididae) in Xinjiang, China. However, the functional significance of honeydew volatiles from <em>A. gossypii</em> for <em>H. variegata</em> remains largely unclear. Here we reported that <em>H. variegata</em> adults were attracted by the crude honeydew of <em>A. gossypii</em>, and we identified fifteen volatiles using headspace solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS). Electroantennogram (EAG) results showed that <em>H. variegata</em> exhibits a significant dose-dependent response to nine volatiles from honeydew. Y-tube olfactometer and cage predation tests showed that 1-heptanol, 2-ethyl-1-hexanol, 2-phenylethanol, and 2-methyl-1-propanol attracted <em>H. variegata</em> adults. Furthermore, our study also elucidated the relationship between honeydew compounds and microorganisms. The findings demonstrated that the compounds 2-ethyl-1-hexanol, 2-phenylethanol, and 2-methyl-1-propanol originate from five distinct cultivable bacteria identified using the 16S rRNA markers. Our results provide potential eco-friendly strategies for enhancing biological control of aphids.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"202 ","pages":"Article 105728"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing strain-specific TaqMan assays for Trichoderma asperellum detection in commercial agricultural settings","authors":"Manfredini Andrea ,&nbsp;Pugliese Massimo ,&nbsp;Valfrè Paolo ,&nbsp;Canfora Loredana","doi":"10.1016/j.biocontrol.2025.105723","DOIUrl":"10.1016/j.biocontrol.2025.105723","url":null,"abstract":"<div><div>The global agricultural sector is facing significant challenges in achieving higher sustainability, which has increased interest in using biological control agents (BCAs) to manage plant diseases. However, it is essential to ensure that microbial-based products, such as BCAs, are utilised in a manner that does not harm soil quality and fertility while decreasing reliance on synthetic pesticides. To accomplish this, it is crucial to monitor the fate and persistence of bioinoculants in the soil, which is essential for optimising their application over time, as well as for regulatory and commercial purposes and environmental risk assessment. A qPCR detection method utilising TaqMan chemistry is proposed, which has demonstrated high specificity and sensitivity in detecting <em>Trichoderma asperellum,</em> a common BCA species, in soil. The primers and probe were designed based on the βtubulin2 gene. The TaqMan-based assay was applied and validated on soils where tomatoes and strawberries were grown after a previous application of <em>T. asperellum</em> FC80 strain over three years. The TaqMan-based assay was able to detect the target strain accurately, meeting the stringent requirements for commercial and regulatory applications.</div></div><div><h3>Significance and impact of the study</h3><div>The TaqMan assay developed here has the potential to impact the agricultural sector significantly. It can be used for regulatory, commercial, and scientific purposes to track, monitor, and determine the presence and fate of <em>T. asperellum</em> under field crop conditions, thereby contributing to adopting more sustainable and efficient agricultural practices.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"202 ","pages":"Article 105723"},"PeriodicalIF":3.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Puccinia punctiformis, a biological control agent, into Cirsium arvense management in semi-arid organic agriculture","authors":"Christian Larson,&nbsp;Daniel Chichinsky,&nbsp;Fabian Menalled,&nbsp;Tim Seipel","doi":"10.1016/j.biocontrol.2025.105724","DOIUrl":"10.1016/j.biocontrol.2025.105724","url":null,"abstract":"<div><div><em>Puccinia punctiformis</em> is an obligate fungal pathogen of <em>Cirsium arvense</em>, a perennial rhizomatous weed common in temperate ecosystems. <em>Cirsium arvense</em> is difficult to manage in organic agriculture and integrated management is necessary to reduce its abundance and impact on crops. <em>Puccinia punctiformis</em> limits <em>C. arvense</em> growth but its effect has not been quantified in cropland field studies. In a four-year organic annual crop sequence at a semi-arid site in Montana, USA, we evaluated (1) <em>P. punctiformis</em> (occurrence, symptomatic stem density) and <em>C. arvense</em> (stem density, relative growth rate [RGR]) in response to tillage (standard vs. reduced), and the relationship between these two variables, and (2) final year crop stem density in response to tillage and <em>P. punctiformis</em> patch infection. Reduced tillage increased <em>P. punctiformis</em> occurrence and symptomatic stem density through time. <em>Cirsium arvense</em> stem density decreased through time in reduced tillage patches that displayed symptomatic infection. <em>Cirsium arvense</em> RGR decreased with increasing years that a patch had <em>P. punctiformis</em> symptomatic infection, and in the reduced tillage treatment regardless of symptoms. Final year crop stem density was higher under standard tillage but increased under reduced tillage with increasing years that a patch had <em>P. punctiformis</em> symptomatic infection. Reduced tillage practices complemented the impact of existing <em>P. punctiformis</em> by increasing its spread and symptomatic infection, two challenges previously identified with using <em>P. punctiformis</em> as a biocontrol agent. When coupled with complementary tactics, producers can integrate <em>P. punctiformis</em> into semi-arid organic agriculture weed management, potentially helping reduce <em>C. arvense</em>’s impact on crops.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"202 ","pages":"Article 105724"},"PeriodicalIF":3.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green guardians: The biocontrol potential of Pseudomonas-derived metabolites for sustainable agriculture","authors":"Ruihuan Yang ,&nbsp;Xiangning Du ,&nbsp;Moein Khojasteh ,&nbsp;Syed Mashab Ali Shah ,&nbsp;Yongzheng Peng ,&nbsp;Zhangfei Zhu ,&nbsp;Zhengyin Xu ,&nbsp;Gongyou Chen","doi":"10.1016/j.biocontrol.2025.105699","DOIUrl":"10.1016/j.biocontrol.2025.105699","url":null,"abstract":"<div><div><em>Pseudomonas</em> is a diverse genus found in various ecological environments, ranging from soil and water to plant surfaces and animal tissues. These bacteria are notable for producing a wide array of bioactive antimicrobial metabolites (AMs), which give them a competitive advantage by inhibiting rival microorganisms and serve as a primary mechanism for the biocontrol of plant diseases, thus supporting sustainable agriculture. This review comprehensively covers recent advancements in the study of <em>Pseudomonas</em>-derived AMs. It discusses the chemical diversity of these metabolites, their broad-spectrum bioactivities against pathogenic bacteria and fungi, oomycetes, nematodes, and their modes of action. The genetic and enzymatic processes involved in AMs production, and the regulatory mechanisms that control these pathways in response to environmental signals. Practical applications of <em>Pseudomonas</em> AMs are also explored, particularly in plant disease management, where they function as biopesticides, reducing dependency on chemical pesticides and promoting sustainable farming practices. This review aims to encourage further exploration into AMs, inspiring new explorations into their production and applications. By highlighting recent progress and emerging trends, it seeks to promote the use of AMs in plant disease management and inspire the development of new green agrochemicals, contributing to more sustainable and environmentally friendly agricultural practices.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"201 ","pages":"Article 105699"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae","authors":"Pavan Kumar ,&nbsp;Giandomenico Corrado ,&nbsp;Girigowda Manjunatha ,&nbsp;Suma Ramegowda ,&nbsp;Pradeep Kumar ,&nbsp;Boris Basile ,&nbsp;Ganadalu Puttaswamy Mutthuraju ,&nbsp;Baggana Girish Jasmitha","doi":"10.1016/j.biocontrol.2024.105686","DOIUrl":"10.1016/j.biocontrol.2024.105686","url":null,"abstract":"<div><div>Plant growth-promoting bacteria (PGPB) have emerged as sustainable tool for managing plant diseases. This study investigates the potential of a <em>Pseudomonas</em>-based biocontrol agent to manage bacterial blight (BB) in pomegranate. This major disease is caused by <em>Xanthomonas axonopodis</em> pv. <em>punicae</em> (Xap) and it is traditionally controlled with antibiotics. Of the 151 bacterial isolates obtained from the pomegranate rhizosphere, three (UHSPS15A, UHSPS33, and UHSPS54) demonstrated the strongest inhibitory effects against Xap <em>in vitro</em>, and their identification as <em>Pseudomonas</em> was confirmed through DNA analysis. Greenhouse trials with Xap-inoculated plants revealed that preventive application of each of the three isolates was more effective than curative, with UHSPS15A providing the highest protection. A talc-based formulation was developed using UHSPS15A. After evaluating its stability and efficacy in greenhouse Xap-inoculated pomegranates plants, open-fields trials indicated that among the three different treatment modes tested, the combined soil and foliar application achieved the highest disease protection and fruit yield, topping the standard antibiotic control. These findings recommend that integrating <em>Pseudomonas</em>-based bio-formulations into disease management strategies could significantly reduce reliance on synthetic chemicals, offering a sustainable alternative for controlling BB in pomegranate.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"201 ","pages":"Article 105686"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective control of southern blight and root rot of Aconitum carmichaelii Debeaux by endophytic Bacillus velezensis YN-2-6S","authors":"Qian Wang ,&nbsp;Yujie Qiu ,&nbsp;Siyu Wang,&nbsp;Yumei Gou,&nbsp;Hongji Hou,&nbsp;Tianwei Su,&nbsp;Lan Zou,&nbsp;Jing Huang","doi":"10.1016/j.biocontrol.2024.105690","DOIUrl":"10.1016/j.biocontrol.2024.105690","url":null,"abstract":"<div><div>Southern blight and root rot were two main soil-borne diseases of <em>Aconitum carmichaelii</em> Debeaux, a famous medicinal plant rich in alkaloids. Beneficial microbe based biological control has been a promising and sustainable alternative for disease control. In this study, an endophytic bacterium YN-2-6S was isolated from the stem of <em>A. carmichaelii</em>. YN-2-6S was identified as <em>Bacillus velezensis</em> by phylogenetic analysis based on concatenated sequence of <em>gyrA</em>, <em>rpoB</em>, and <em>recA</em> genes. YN-2-6S showed strong antagonistic capacity against <em>Sclerotium rolfsii</em> and <em>Fusarium oxysporum</em> both <em>in vitro</em> and on root slices of <em>A. carmichaelii</em> by dual-culture assay. Field experiment revealed that YN-2-6S culture significantly reduced southern blight and root rot disease occurrences on <em>A. carmichaelii</em>, with 91.00 % and 85.71 % biocontrol efficiency respectively. The biocontrol activity persisted at least for 54 days. In addition, YN-2-6S inoculation significantly increased fresh and dry weight of stems, main roots and lateral roots of <em>A. carmichaelii</em> compared with non-inoculation and commercial <em>B. subtilis</em> treatments. YN-2-6S inoculation significantly promoted the accumulation of total alkaloids content of <em>A. carmichaelii</em> lateral roots to 17.36 mg/g. Cell-free culture filtrate of YN-2-6S showed strong inhibitory effect against <em>S. rolfsii</em> and <em>F. oxysporum in vitro</em>. The presence of <em>ituC</em>, <em>fenA</em>, <em>fenB</em>, <em>fenD</em>, <em>srfAA</em>, <em>bmyB</em>, <em>bacA</em>, <em>baeA</em>, <em>mnlA</em>, and <em>dfnA</em> genes by PCR amplification indicated that YN-2-6S was able to produce antifungal lipopeptides and polyketides. Furthermore, volatile compounds released by YN-2-6S also significantly inhibited the growth of <em>S. rolfsii</em> and <em>F. oxysporum in vitro</em>. In addition, YN-2-6S was able to produce glucanase, cellulase, protease, indole-3-acetic acid with growth-promoting ability. Increase of peroxidase (POD) and catalase (CAT) enzyme activity of <em>A. carmichaelii</em> leaves was detected upon inoculation of YN-2-6S, which indicated that YN-2-6S may induce systemic resistance on <em>A. carmichaelii</em>. Taken together, this study proved that YN-2-6S was a promising biocontrol agent against southern blight and root rot of <em>A. carmichaelii</em> and the underline mechanism may involve the production of antifungal lipopeptides, polyketides, volatile compounds, hydrolysis and induction of host systemic resistance.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"201 ","pages":"Article 105690"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol of gray spot disease on Polygonatum cyrtonema Hua by Bacillus velezensis GX1","authors":"L. Sun ,&nbsp;G.L. Deng ,&nbsp;H.L. Wu ,&nbsp;Q. He ,&nbsp;S.H. Hu ,&nbsp;J.Y. Li ,&nbsp;X.Y. Li ,&nbsp;S. Meng ,&nbsp;A.N. He ,&nbsp;Y.Q. Tian ,&nbsp;X. Chen ,&nbsp;S.H. Li","doi":"10.1016/j.biocontrol.2025.105704","DOIUrl":"10.1016/j.biocontrol.2025.105704","url":null,"abstract":"<div><div>This study evaluated the biocontrol efficacy of <em>Bacillus velezensis</em> GX1 against gray spot disease (<em>Pestalotiopsis xuefengensis</em>) on the horticultural species, <em>Polygonatum cyrtonema</em> Hua. Results indicated that GX1 effectively inhibited <em>P. xuefengensis</em> HJHB1, inhibiting growth of the pathogen by 74.97 % <em>in vitro</em> and inhibiting disease development by 87.13 % in field experiments. Notably, a 10 % (v/v) concentration of cell-free supernatant (CFS) of GX1 fully inhibited mycelial growth. An extract of lipopeptides (LPs) prepared at 60 μg/ml strongly inhibited mycelial growth and spore germination and caused morphological deformation of the mycelia. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that strain GX1 produced significantly higher levels of surfactin A, surfactin B and plipastatin, relative to strain GX8, an ineffective strain. Commercial preparations of plipastatin and surfactin indicated that plipastatin exhibited strong antifungal activity but surfactin did not. A bacterial solution of GX1 had no significant effect on superoxide dismutase (SOD) activity in <em>P. cyrtonema</em> but did enhance the activity of peroxidase (POD) and decreased catalase (CAT) activity, which are associated with increased resistance of <em>P. cyrtonema</em> to gray spot disease.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"201 ","pages":"Article 105704"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Legume-derived phenolic acids influence Fusarium oxysporum f.sp. strigae compatibility and biocontrol potential in a Striga push–pull system","authors":"Mekuria Wolde Assena ,&nbsp;Jochen Schöne ,&nbsp;Frank Rasche","doi":"10.1016/j.biocontrol.2025.105721","DOIUrl":"10.1016/j.biocontrol.2025.105721","url":null,"abstract":"<div><div>Biological control of <em>Striga hermonthica</em> (Del.) Benth. includes push–pull, intercropping using legumes, and soil-borne fungi such as <em>Fusarium oxysporum</em> f.sp. <em>strigae</em> (FOS). Efficacy of both technologies is compromised by various environmental factors. Combining them could potentially overcome their limitations and enhance control of <em>S. hermonthica</em>. It was hypothesized that FOS inoculation is compatible with push–pull intercropping, allowing consistent <em>S. hermonthica</em> suppression. Effects of selected phenolic acids and crude root exudates from three legume species (<em>Mucuna pruriens</em>, <em>Desmodium uncinatum</em>, <em>Crotalaria juncea</em>) on FOS mycelial growth and spore germination were studied. Bioassays showed that most phenolic acids (e.g., caffeic, <em>p</em>-hydroxybenzoic, syringic) had no effect on FOS development, except for piperonylic (80 %, 38 %) and <em>t</em>-cinnamic (53 %, 33 %) acids, which strongly inhibited growth at concentrations of 1 and 0.5 mM, respectively. Crude root exudates from <em>Crotalaria</em> inhibited FOS mycelial growth at 1 (44 %) and 0.5 (32 %) mg ml^-1. <em>Desmodium</em> and <em>Mucuna</em> exudates showed no inhibition. Greenhouse results showed that legume intercropping and FOS inoculation reduced <em>S. hermonthica</em> emergence, with their integration achieving over 95 % suppression and boosting maize biomass. Legume presence enhanced FOS proliferation, with <em>Mucuna</em> and <em>Desmodium</em> increasing FOS gene copy numbers by over 44 %. LC-MS analysis identified nine phenolic acids, with <em>p</em>-coumaric (85–128 µg g⁻¹) and <em>p</em>-hydroxybenzoic (50–97 µg g⁻¹) acid being most abundant; the latter positively correlated with FOS abundance. In this setting, legume intercropping with maize did not impair FOS proliferation in rhizosphere soil and effectively suppressed <em>S. hermonthica</em>, demonstrating compatibility of FOS inoculation with push–pull for effective <em>S. hermonthica</em> management.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"202 ","pages":"Article 105721"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae","authors":"Cristiana Correia ,&nbsp;Antonio Cellini ,&nbsp;Irene Donati ,&nbsp;Panagiotis Voulgaris ,&nbsp;Adebayo Ebenezer Obafemi ,&nbsp;Elia Soriato ,&nbsp;Elodie Vandelle ,&nbsp;Conceição Santos ,&nbsp;Francesco Spinelli","doi":"10.1016/j.biocontrol.2025.105706","DOIUrl":"10.1016/j.biocontrol.2025.105706","url":null,"abstract":"<div><div><em>Pseudomonas syringae</em> pv. <em>actinidiae</em> (Psa), the etiological agent of the bacterial canker in <em>Actinidia</em> plants, remains the main threat to kiwifruit orchards worldwide. Though <em>e</em>nvironment-friendly disease control methods based on biological control agents (BCAs) represent a promising alternative to xenobiotic pesticides, their efficacy in field conditions has often resulted erratic. The selection of beneficial microorganisms directly from the phyllosphere of the host plant is a promising approach to overcome this limitation since it ensures the adaptation of the isolates to the environment in which they are going to be applied. This work reports the screening of the kiwifruit epiphytic bacterial community from three Psa infected orchards in Portugal to identify potential bacterial BCAs capable of inhibiting Psa growth or interfering with its virulence. Strains of <em>Pseudomonas putida</em> and <em>Pseudomonas azotoformans</em> efficiently antagonized Psa on flowers and leaves and colonized all susceptible organs with high surviving rates in glasshouse conditions. <em>In vitro</em> metabolic analysis together with genome sequencing and annotation revealed siderophore production, in particular pyoverdine, which may limit iron availability to the pathogen. Moreover, several biosynthetic gene clusters of secondary metabolites, were predicted in the genome of both strains, including non-ribosomal peptides, and the bacteriocin pyocin was predicted in the genome of BG1. Overall, these results open new perspectives to develop commercial products for Psa management based on kiwifruit-native bacteria, well-adapted to common orchard management practices, with a high efficiency of host plant colonization, at Psa-conducive temperatures, and point out possible mechanisms of action for these two BCA candidates, supporting further steps to assess their effectiveness in orchard conditions.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"201 ","pages":"Article 105706"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}