Biological Control最新文献

{"title":"Discovery of novel non-live bacterial bioinsecticides for mosquito control through a high-throughput screening pipeline","authors":"Madhavinadha Prasad Kona ,&nbsp;Vandana Vandana ,&nbsp;Jenny Wang ,&nbsp;Qiran Amy Dong ,&nbsp;F. Elektra Maridaki ,&nbsp;George Dimopoulos","doi":"10.1016/j.biocontrol.2026.105987","DOIUrl":"10.1016/j.biocontrol.2026.105987","url":null,"abstract":"<div><div>The growing threat of insecticide resistance and ecological harm from synthetic insecticides underscores the urgent need for new sustainable mosquito control strategies. In this study, we implemented a systematic high-throughput screening (HTS) of non-live bacterial cultures to identify novel bacterial strains with potent mosquito larvicidal activity. From 48 environmental samples collected in a tropical environment on the southeast coast of Puerto Rico, 486 bacterial isolates were obtained, of which 30 exhibited significant killing-activity as non-live desiccated cultures, against <em>Aedes aegypti</em> larvae. Notably, three isolates achieved 100% larval mortality within 24 h. Further bioassays confirmed dose-dependent efficacy across <em>Aedes aegypti</em>, <em>Culex quinquefasciatus</em>, and <em>Anopheles stephensi</em>, indicating broad-spectrum larvicidal activity. Taxonomic analysis revealed diverse genera, including <em>Enterobacter</em>, <em>Bacillus</em>, <em>Serratia</em>, <em>Acinetobacter</em>, <em>Pseudomonas</em>, <em>Chromobacterium</em>, <em>Pantoea</em>, <em>Staphylococcus</em>, and <em>Raoultella</em>. Crucially, the larvicidal effect was retained in non-live bacterial biomass after heat inactivation, highlighting practical advantages with regard to stability, safety, and deployment over live biocontrol agents. Comparative assays of different formulations further indicated that delivering bacterial biomass in pellet form can enhance larval mortality compared to suspended powder in the larval breeding water, likely due to improved retention and localized concentration of active metabolites. Collectively, this study establishes a robust discovery pipeline for environmentally friendly, microbe-derived non-live bioinsecticides to support integrated mosquito vector management.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105987"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographic origin of a factitious host shapes parasitoid performance in Trichogramma chilonis–Antheraea pernyi systems","authors":"Talha Tariq ,&nbsp;Zhen Shen ,&nbsp;Haneef Tariq ,&nbsp;Yong-Ming Chen ,&nbsp;Nicolas Desneux ,&nbsp;Lian-Sheng Zang","doi":"10.1016/j.biocontrol.2026.105991","DOIUrl":"10.1016/j.biocontrol.2026.105991","url":null,"abstract":"<div><div>Geographical variation can profoundly influence host-parasitoid compatibility and mass-rearing performance in biological control programs. We assessed how the geographical origin of the Chinese oak silkworm (<em>Antheraea pernyi</em>) affects the reproductive performance of the egg parasitoid <em>Trichogramma chilonis</em>. Two <em>A. pernyi</em> populations from Jilin (JCOS) and Guizhou (GCOS) provinces, and two <em>T. chilonis</em> populations from the same regions (JL-TC and GZ-TC), were reciprocally tested to evaluate host suitability. Morphometric analyses revealed that JCOS exhibited a larger body size, a longer wingspan, and thicker eggshells than GCOS, indicating regional morphological differentiation. Parasitism and emergence rates were significantly higher on GCOS eggs (82–86% and 67–76%, respectively) than on JCOS eggs (70–75% and 41–47%), primarily because their thinner eggshells facilitated oviposition and adult emergence. Development duration was shorter on GCOS eggs, while the proportion of female progeny remained consistently high (&gt; 85%) across treatments. Although <em>T. chilonis</em> populations showed comparable parasitism levels, the JL-TC strain produced slightly more female offspring than GZ-TC, suggesting minor adaptive divergence. Regression analyses demonstrated a strong negative correlation between eggshell thickness and parasitoid emergence, confirming eggshell structure as a critical determinant of host suitability. These findings highlight that the geographical origin and morphological traits of <em>A. pernyi</em> influence the reproductive success of <em>T. chilonis</em>. Selecting optimal host populations, such as GCOS, can enhance parasitoid production efficiency and reliability, improving the sustainability of mass-rearing systems and biological control programs.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"214 ","pages":"Article 105991"},"PeriodicalIF":3.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147403541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating astigmatid mites as alternative diets for Orius laevigatus: Effects on life history parameters","authors":"Amador Rodríguez-Gómez ,&nbsp;Ana Belén Abelaira ,&nbsp;María del Carmen Reche ,&nbsp;Virginia Balanza ,&nbsp;Pablo Bielza","doi":"10.1016/j.biocontrol.2025.105956","DOIUrl":"10.1016/j.biocontrol.2025.105956","url":null,"abstract":"<div><div>Integrated pest management (IPM) in greenhouse cultivation has benefited significantly from the use of predators such as <em>Orius laevigatus</em>. This beneficial insect, a natural enemy of thrips, can feed on pollen, facilitating preventive releases in flowering crops. However, in the absence of quality pollen, a supplementary food source is required to facilitate establishment. This study compared the performance of different populations of <em>O. laevigatus</em>, including wild and commercial populations and selected strains adapted to suboptimal pollen diets (1POL and 2POL), all fed with various species of astigmatid mites, a more economical alternative to traditional foods like <em>Ephestia kuehniella</em> eggs and <em>Artemia</em> cysts. The selected strains of <em>O. laevigatus</em> exhibited higher survival during nymphal development and greater fecundity when fed astigmatid mites compared to commercial populations. Among the astigmatid mites, <em>Acarus siro</em> was the best-performing diet, supporting high survival and acceptable development; however, it did not match the fecundity obtained with <em>Ephestia kuehniella</em> eggs. <em>Acarus siro</em> therefore represents a cost-effective supplementary or maintenance food, particularly suitable for sustaining populations in crops and for reducing reliance on <em>Ephestia</em>, especially when using genetically selected <em>O. laevigatus</em> strains that show enhanced performance on suboptimal diets.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105956"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sugar supplementation affects ant-mealybug mutualism and promotes natural enemies for mealybug control in pineapple systems","authors":"Marie Bourel ,&nbsp;Eva Faustin ,&nbsp;Bernard Raymond Abufera ,&nbsp;Capucine Perrey ,&nbsp;Camille Stasiolojc ,&nbsp;Anaïs Chailleux ,&nbsp;Felix Wäckers ,&nbsp;Philippe Tixier ,&nbsp;Dominique Carval","doi":"10.1016/j.biocontrol.2026.105975","DOIUrl":"10.1016/j.biocontrol.2026.105975","url":null,"abstract":"<div><div>Honeydew-producing hemipterans establish mutualistic interactions with ants, which collect honeydew while providing protection against natural enemies and other services. Although this association benefits hemipterans, it often exacerbates crop damage by increasing pest abundance. Providing alternative sugar resources has been proposed as a strategy to disrupt these associations, but its effectiveness remains poorly tested in tropical agrosystems. Here, we investigated whether sugar feeders could reduce ant-mealybug mutualism in pineapple (<em>Ananas comosus</em>) fields on La Réunion, where the mealybug <em>Dysmicoccus brevipes</em>, a vector of Pineapple Mealybug Wilt-associated viruses (PMWaVs), poses a major threat to production. We deployed sugar feeders in replicated field plots and monitored ant, mealybug and predator abundances over eleven months, complemented by sentinel prey assays to quantify ant-mealybug interaction rates and mealybug removal. Mealybug abundance was positively associated with ant presence, while sugar supplementation reduced ant abundance by 42% on pineapple plants compared to controls. Predator abundance was 105% higher in supplemented zones, likely due to both direct nutritional benefits and lower ant interference, but declined with distance from feeders. Three ant species, <em>Solenopsis geminata</em>, <em>Pheidole megacephala</em> and <em>Brachymyrmex cordemoyi</em> accounted for most interactions with mealybugs, with species-specific temporal dynamics but no response to sugar supplementation. Sugar supplementation represents a promising management lever, but its effect on mealybug remains context-dependent. Integrating sugar provisioning with complementary agroecological practices, such as biological control and habitat diversification, may maximize its impact and contribute to sustainable management of pineapple mealybug wilt disease.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105975"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biocontrol potential of Streptomyces sp. B2008 through mechanistic insights into the antifungal effect of actinomycin D","authors":"Wenjin Hu ,&nbsp;Yuying Huang ,&nbsp;Xiaochun Wang ,&nbsp;Jinling Qin ,&nbsp;Wenjun Li ,&nbsp;Baoshen Li ,&nbsp;Xinli Pan","doi":"10.1016/j.biocontrol.2026.105968","DOIUrl":"10.1016/j.biocontrol.2026.105968","url":null,"abstract":"<div><div>Our previous study revealed that a mangrove-sediments derived actinomycete, <em>Streptomyces</em> sp. B2008, could produce antibiotic actinomycin D and effectively inhibit the growth of <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> tropical race 4 (<em>Foc</em> TR4)<em>,</em> the most destructive pathogen of banana worldwide. Here, the growth rate of banana plants was significantly accelerated after the application of strain B2008. Actinomycin D isolated from the strain displayed potent antifungal activity against <em>Foc</em> TR4 with an EC₅₀ value of 26 μg mL⁻¹ that may contribute to its antagonist activity. Transcriptomic and molecular docking analysis revealed that actinomycin D was able to regulate gene expressions of <em>FOIG_09283, FOIG_02707</em> and <em>FOIG_05942</em> in <em>Foc</em> TR4<em>.</em> Deletion of these genes led to phenotype featured with severe defects in hyphae growth and colony morphology, while weakening environmental stress tolerance. Furthermore, lack of <em>FOIG_02707</em> and <em>FOIG_05942</em> showed a defect in conidiation and remarkably affected the pathogenicity of <em>Foc</em> TR4. These findings suggested that actinomycin D inhibited <em>Foc</em> TR4 by affecting expression of <em>FOIG_02707</em> and <em>FOIG_05942</em>, further confirming the application potential of <em>Streptomyces</em> sp. B2008 as a biological control agent.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105968"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control of Bacillus licheniformis PR2 against Fusarium oxysporum and growth promotion of Pinus strobus L. as an urban greening strategy","authors":"Jae-Hyun Moon,&nbsp;Henry B. Ajuna,&nbsp;Su-In Choi,&nbsp;Ju-Yeol Yun,&nbsp;Su-Yeon Lee,&nbsp;Vantha Choub,&nbsp;Young Sang Ahn","doi":"10.1016/j.biocontrol.2026.105966","DOIUrl":"10.1016/j.biocontrol.2026.105966","url":null,"abstract":"<div><div>Eastern white pine (<em>Pinus strobus</em> L.) is an evergreen conifer with a beautiful rounded pyramidal canopy, growing up to 80 feet tall and 40 feet wide, with a lifespan of approximately 200 years, making it an excellent tree for urban greenery, shade, windbreak, living fence/hedge, as well as for crafts and outdoor Chrismas decorations worldwide. However, it is highly susceptible to notorious fungal pathogens such as <em>Fusarium oxysporum</em> at various of growth, from nursery seedlings to mature trees in the field, causing lasting effects such as growth retardation or death. Therefore, this study aimed to evaluate the potential of <em>Bacillus licheniformis</em> PR2 as an alternative to chemical fungicides and fertilizers in the management of <em>Fusarium</em> infections and the promotion of growth of <em>P. strobus</em> seedlings in the nursery. <em>B. licheniformis</em> PR2 produced fungal cell wall-degrading enzymes such as chitinase, β-1,3-glucanase and protease, which degraded chitin, β-glucan and glycoprotein polymers that constitute the cell wall of <em>F. oxysporum</em>. Consequently, the bacterial treatment inhibited spore germination and mycelial growth of <em>F. oxysporum</em> by 85.9% and 61.5%, respectively, at 100% concentration of the crude enzyme fraction. The nursery experiment, treatment of <em>P. strobus</em> seedlings with <em>B. licheniformis</em> PR2 culture increased the survival rate of seedlings by 2.3- and 2.1-fold compared to chemical fertilizer treatment and the control groups, respectively. In addition, <em>B. licheniformis</em> PR2 produced up to 108.9 mg/mL of indole-3-acetic acid (IAA), a plant hormone that promotes root growth and lateral root formation, thereby promoting the uptake of water and nutrients by seedlings. Consequently, treatment of <em>P. strobus</em> seedlings with the bacterial culture of <em>B. licheniformis</em> PR2 improved the root collar diameter, dry weight of shoots and roots compared to the chemical fertilizer treatment and control group, respectively. Therefore, this study demonstrates that <em>B. licheniformis</em> PR2 could be applied as an eco-friendly alternative to chemical fungicides and fertilizers in the sustainable development of urban green spaces, since it can effectively suppress fungal pathogens and promote seedling growth without leaving harmful environmental residues or posing a healthy risk to urban residents.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105966"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pleiotropic regulatory function of two component system CbrAB in rhizosphere bacterium Pseudomonas protegens FD6","authors":"Jieyu Hu,&nbsp;Yinyin Liu,&nbsp;Cheng Wang,&nbsp;Tao Wu,&nbsp;Qingxia Zhang","doi":"10.1016/j.biocontrol.2026.105958","DOIUrl":"10.1016/j.biocontrol.2026.105958","url":null,"abstract":"<div><div><em>Pseudomonas protegens</em> FD6 is an effective biocontrol agent that confers protection against tomato bacterial wilt disease caused by <em>Ralstonia solanacearum</em>. In a two-component system, CbrAB regulated different physiological functions to adapt to diverse environments in pseudomonads and <em>Azotobacter</em> spp.. Here, we explored the global regulatory function of CbrAB on biocontrol-associated traits in <em>P. protegens</em> FD6. CbrAB is involved in several functions, such as antibiotic production, motility, biofilm formation and stress response. Mutation in CbrAB system leads to a loss of the ability to control tomato bacterial wilt and growth defects in LB broth during the logarithmic phase. Additionally, CbrAB might regulate 2,4-diacetylphloroglucinol (2,4-DAPG) and pyoluteorin (PLT) production. The luminescence of luciferase reporter system data suggested that CbrA positively modulates the transcription of the antibiotic synthesis genes <em>pltL</em> and <em>phlA.</em> CbrB differentially regulates the expression of <em>pltL</em> and <em>phlA</em> at the transcriptional level, which is consistent with the results of the quantitative analysis of antibiotics. In this study, we also show that <em>cbrA</em> and <em>cbrB</em> contribute to the efficient colonization of tomato roots by <em>P. protegens</em> FD6. Compared with the wild type, both <em>cbrA</em> and <em>cbrB</em> mutants were also less competitive on tomato roots. We further revealed <em>cbrB</em> positively regulated transcription of <em>algD</em> and <em>flgB,</em> responsible for alginate synthesis and swimming motility<em>,</em> respectively, and sRNAs <em>crcY</em> and <em>crcZ</em> by directly binding to their upstream sequences. Overall, CbrAB in <em>P. protegens</em> contributes to its biological control activity, persistence during root colonization and adaptation to complicated environments.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105958"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-target effects of Bacillus thuringiensis and Metarhizium anisopliae on egg parasitoids of Spodoptera frugiperda","authors":"Yuanyuan Cheng ,&nbsp;Hongmei Li ,&nbsp;Xianming Yang ,&nbsp;Suqin Shang ,&nbsp;Abdul Aziz Bukero ,&nbsp;Feng Zhang","doi":"10.1016/j.biocontrol.2026.105957","DOIUrl":"10.1016/j.biocontrol.2026.105957","url":null,"abstract":"<div><div>Biological control is regarded as the most important green measures to control agricultural pests. It is valuable to understand the compatibility between microbial pesticides and natural enemies. This study aimed to assess the non-target effects on <em>Trichogramma chilonis</em>, a predominant egg parasitoid of the major maize pest <em>Spodoptera frugiperda</em> (fall armyworm, FAW) by two commonly used maize field biopesticides: <em>Bacillus thuringiensis</em> G033A (<em>Bt</em>) and <em>Metarhizium anisopliae</em> CQMa421. In laboratory and semi-field experiments guided by IOBC protocols, neither biopesticide interfered with the parasitism efficiency or developmental duration of <em>T. chilonis</em> when applied before parasitism. Similarly, post-parasitism application didn’t affect the parasitoid’s host-seeking behavior. However, <em>Bt</em> application on parasitized eggs resulted in a 23.5 % reduction in adult parasitoid emergence rate, though the magnitude of this effect remained below the IOBC safety threshold (&lt;30 %). There was no harm found on the parasitoids by <em>M. anisopliae</em> under all tested conditions. Thus, both biopesticides are largely compatible with <em>T. chilonis</em> for FAW control. The study disclosed that it is necessary to consider the optimal timing for the use of microbial pesticides and the release of natural enemies to enhance their synergistic pest control efficacy in field conditions. <em>Bt</em> application should be avoided immediately after parasitoids release.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105957"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indirect effects of beneficial fungal treatments on pest insects can vary with plant genotype and spore viability","authors":"Cesar U. Valencia ,&nbsp;Janaina C.S. Cunha ,&nbsp;Loren Rivera-Vega ,&nbsp;Gregory A. Sword","doi":"10.1016/j.biocontrol.2025.105953","DOIUrl":"10.1016/j.biocontrol.2025.105953","url":null,"abstract":"<div><div>Fungal entomopathogens as biopesticides are typically applied as foliar sprays and assessed for their insecticidal activity. However, plant responses to these microbial treatments remain poorly understood. Additionally, environmental conditions such as desiccation and UV exposure can reduce spore viability, resulting in both live and dead spores serving as potential elicitors of plant responses. Whether foliar treatments with viable or dead fungal spores influence cotton defenses against the cotton aphid, <em>Aphis gossypii</em>, was studied. Spores of <em>Beauveria bassiana</em>, <em>Phialemonium inflatum</em>, and <em>Chaetomium globosum</em> were tested on two cotton genotypes for effects on aphid reproduction. Viable spores did not alter aphid population growth on either genotype. In contrast, dead spores significantly increased aphid populations on one genotype. Treatments with plant defense elicitors, Methyl Jasmonate and Actigard 50WG, increased aphid populations to levels observed with heat-killed spore treatments, suggesting that dead spores may activate plant defense pathways that inadvertently benefit aphids. Foliar application of chitosan, a proxy for fungal chitin, further indicated that plant genotype-specific responses to Microbe-Associated Molecular Patterns (MAMPs) likely underlie the observed differential aphid responses. Together, these findings demonstrate that heat-killed fungal spores can modulate plant defenses, thereby influencing the performance of insect herbivores. Our results highlight how beneficial fungal applications may alter host plant suitability by inducing plant defense pathways, potentially with unintended consequences for insect pest populations.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105953"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tempo-defined resilience: Early Bacillus amyloliquefaciens F11 application reshapes rhizosphere microbiome to suppress tobacco bacterial wilt","authors":"Ji Yang ,&nbsp;Hainian Chen ,&nbsp;Chaowen Li ,&nbsp;Benfu Cao ,&nbsp;Xianghong Zeng ,&nbsp;Xiang Li ,&nbsp;Shengliang Zhang ,&nbsp;Yingang Lu ,&nbsp;Li Liu ,&nbsp;Sanwei Yang","doi":"10.1016/j.biocontrol.2025.105954","DOIUrl":"10.1016/j.biocontrol.2025.105954","url":null,"abstract":"<div><div>Therapeutic application of Bacillus amyloliquefaciens F11 during initial symptom onset suppressed tobacco bacterial wilt with 32 % control efficacy, outperforming both diseased controls and streptomycin treatment. STAMP analysis revealed that F11 treatments (B and I) substantially reduced key pathogens including <em>Ralstonia</em> and <em>Acidobacterium,</em> a suppression not achieved by streptomycin. The biocontrol mechanism likely combined direct pathogen suppression targeting Ralstonia solanacearum and Aquicella with enrichment of beneficial taxa including Stenotrophomonas and Rhizobium, leading to structural reorganization of the rhizosphere microbiome as confirmed by β-diversity shifts (PERMANOVA, P = 0.001). Our comparative indicator taxa analysis identified eight microbial indicators, including disease-suppressive members (Stenotrophomonas, Rhizobium, and Sphingobacterium), and disease-conducive guild (Ralstonia, Metallibacterium, Rhizomicrobium, Asticcacaulis, and Rhodocista), overcoming the limitations of conventional biomarker approaches LEfSe. Notably, F11 application disrupted the co-occurrence pattern between Ralstonia and Metallibacterium, indicating its capacity to interfere with potentially pathogenic associations. These findings demonstrate that temporally optimized F11 application rebuilds disease-suppressive soils through targeted microbiome restructuring, providing a sustainable alternative to chemical dependent disease management.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"213 ","pages":"Article 105954"},"PeriodicalIF":3.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145908798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}