Biological Control最新文献

{"title":"A genetically safe Burkholderia cenocepacia strain P3 suppresses Cercospora zeina via siderophore-mediated iron deprivation","authors":"Lu Zheng,&nbsp;Rui Chen,&nbsp;Xin Pu,&nbsp;Ting Zheng,&nbsp;Bing He","doi":"10.1016/j.biocontrol.2025.105896","DOIUrl":"10.1016/j.biocontrol.2025.105896","url":null,"abstract":"<div><div>Gray leaf spot (GLS), caused by <em>Cercospora zeina</em>, is a devastating fungal disease of maize worldwide. This study reports the isolation of a novel rhizobacterium, strain P3, from the maize rhizosphere, which was identified as <em>Burkholderia cenocepacia</em> based on phylogenetic analysis of 16S rRNA and dnaG genes. Strain P3 exhibited broad-spectrum antifungal activity against multiple pathogens and demonstrated plant growth-promoting traits. Genomic analysis confirmed the absence of major virulence genes, supporting its environmental safety. Fermentation conditions for siderophore production were optimized, achieving a yield of 85.5 % siderophore units under optimal conditions (sucrose 5 g/L, asparagine 3 g/L, pH 7.0, 32 °C). The fermentation supernatant of P3 inhibited <em>C. zeina</em> growth dose-dependently, causing hyphal membrane damage and reactive oxygen species accumulation. Two siderophores, pyochelin and ornibactin, were identified and shown to synergistically suppress fungal growth under iron-limited conditions. Greenhouse trials demonstrated that pretreatment with strain P3 significantly reduced lesion area and disease severity in maize plants challenged with <em>C. zeina</em>. These results indicate that B. cenocepacia P3 is a promising, environmentally friendly biocontrol agent with strong potential for integrated management of gray leaf spot.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105896"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entomopathogenic fungi from paddy soils suppress a major insect pest and enhance rice growth under greenhouse conditions","authors":"Noppol Kobmoo ,&nbsp;Suchada Mongkolsamrit ,&nbsp;Artit Khonsanit ,&nbsp;Wasana Noisripoom ,&nbsp;Non Sawangkaew ,&nbsp;Donnaya Thanakitpipattana ,&nbsp;Cattarin Theerawitaya ,&nbsp;Suriyan Cha-um ,&nbsp;Janet Jennifer Luangsa-ard ,&nbsp;Jintana Unartngam","doi":"10.1016/j.biocontrol.2025.105894","DOIUrl":"10.1016/j.biocontrol.2025.105894","url":null,"abstract":"<div><div>Intensive agrochemical use in rice cultivation poses environmental and health risks, emphasizing the need for sustainable alternatives. The development of biocontrol agents that can simultaneously suppress pests and enhance rice growth and production is particularly needed. In this study, we investigated the functionality of Hypocrealean entomopathogenic fungi (EPF) isolated from paddy soils across Thailand to suppress a major insect pest, the brown planthopper (<em>Nilaparvata lugens</em>) and to promote rice growth. Seventy-five EPF strains were isolated from paddy soils, primarily identified as belonging to <em>Metarhizium</em> and <em>Purpureocillium</em> genera. Two <em>Metarhizium</em> strains (MY13317.02 and MY13317.32), belonging to <em>M. pingshaense</em> sensu lato complex, exhibited strong virulence against <em>N. lugens</em> in vitro and demonstrated high phosphate-solubilizing activity, significantly enhancing rice growth, yield and photosynthetic performance under greenhouse conditions. The findings highlight the promising potential of these <em>Metarhizium</em> strains as multifunctional bioinoculants for sustainable rice agriculture, combining plant growth promotion with biocontrol efficacy.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105894"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional response and control efficacy of Plesiochrysa ramburi and Apertochrysa roseusfrontata (Neuroptera: Chrysopidae) on the Grey Pineapple Mealybug","authors":"Ziyuan Li ,&nbsp;Linlin Shi ,&nbsp;Jian Wen ,&nbsp;Yanmei Zhang ,&nbsp;Kewei Chen","doi":"10.1016/j.biocontrol.2025.105899","DOIUrl":"10.1016/j.biocontrol.2025.105899","url":null,"abstract":"<div><div>The gray pineapple mealybug, <em>Dysmicoccus neobrevipes</em> Beardsley (Hemiptera: Pseudococcidae), causes damage through sap-sucking, honeydew secretion, and potentially transmission of pineapple mealybug wilt-associated virus (PMWaV), thereby threatening the sustainability of China’s sisal industry. This study assessed the biological control potential of two predatory lacewings, <em>Plesiochrysa ramburi</em> (Schneider) and <em>Apertochrysa roseusfrontata</em> Wang &amp; Li &amp; Liu, collected from sisal plantations in Zhanjiang (Guangdong Province, China), by evaluating their functional responses and greenhouse control efficacy against <em>D. neobrevipes</em>. Laboratory assays showed that all larval instars of <em>P. ramburi</em> exhibited Type II functional response, whereas <em>A. roseusfrontata</em> displayed such responses only in the 2nd and 3rd instars. Across all stages, <em>P. ramburi</em> exhibited significantly higher predation rates than <em>A. roseusfrontata</em>. Greenhouse trials further revealed that releases of the 3rd instar larvae from both species at predator-pest ratios of 1:10, 1:15, and 1:20 reduced <em>D. neobrevipes</em> populations by over 80 % within 14 days. Specifically, inundative releases after mealybug infestation at a ratio of 1:10 reduced populations by 98.58 % for <em>P. ramburi</em> and 96.52 % for <em>A. roseusfrontata</em>. Overall, all larval stages of both species were effective predators of <em>D. neobrevipes</em>, with <em>P. ramburi</em> showing superior performance. Both inoculative and inundative release of <em>P. ramburi</em> larvae effectively controlled <em>D. neobrevipes</em> in greenhouses, highlighting its promise as a candidate for biological control.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105899"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genomics reveals global patterns of genetic diversity and population structure associated with human-mediated spread of the invasive vine, Cape ivy (Delairea odorata Lem.): Implications for biological control research","authors":"Gavin C. Hunter ,&nbsp;Daniella Egli ,&nbsp;Francisco Encinas-Viso ,&nbsp;Kent F. McCue ,&nbsp;Scott L. Portman ,&nbsp;Isabel Zeil-Rolfe ,&nbsp;Patrick J. Moran ,&nbsp;Ben Gooden","doi":"10.1016/j.biocontrol.2025.105893","DOIUrl":"10.1016/j.biocontrol.2025.105893","url":null,"abstract":"<div><div>We investigated global patterns of population genetic diversity and structure for the clonal invasive scrambler, Cape ivy (<em>Delairea odorata</em> Lem., family Asteraceae), to help optimise exploratory surveys of candidate biocontrol agents in its native range, South Africa. We collected more than 1000 Cape ivy samples from 122 populations throughout South Africa and its introduced ranges (Australia, New Zealand, mainland USA, Hawai’i and southern France), and performed population genetic diversity and structure analyses based on single nucleotypic polymorphisms (SNPs). On average, population genetic diversity values were six times higher in South Africa than all introduced populations, although these values were three times higher for the mainland USA populations than those in Australia, Hawai’i and New Zealand. Populations were strongly differentiated with limited gene flow between the native and introduced ranges (intercontinental scale) and between sites within each range (regional scale). All Australian, New Zealand, Hawai’ian and some Californian populations had Fst values approaching zero, likely arising from a single clonal accession derived from the same ancestral population, likely from the Western Cape Province of South Africa. The remaining mainland USA populations showed signs of multiple introductions and admixture patterns. Our results show that human-assisted dispersal of Cape ivy through the ornamental plant trade can overcome genetic (bottleneck) barriers to global invasion, especially for clonal accessions of the plant. We recommend that host-specificity testing of candidate biocontrol agents for Cape ivy in the Australian and New Zealand contexts should source agents from the Western Cape Province of South Africa.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105893"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of Fusarium spp. associated with red stele root rot of strawberry (Fragaria ananassa) in northern Zhejiang, China","authors":"Yiming Zhang ,&nbsp;Minyan Song ,&nbsp;Shiliu Zhu ,&nbsp;Yehua Si","doi":"10.1016/j.biocontrol.2025.105876","DOIUrl":"10.1016/j.biocontrol.2025.105876","url":null,"abstract":"<div><div>In recent years, strawberry red–stele root rot has caused significant economic losses for growers in northern Zhejiang, China. Isolates were identified using morphological traits and molecular biological analysis,Koch’s postulates was established by re-isolation of strains from the inoculated roots. Biological characteristics were determined, and fungicide sensitivity was assessed <em>in vitro</em> with the mycelial–growth‐rate assay. Two species—<em>Fusarium oxysporum</em> and <em>F. solani</em>—were confirmed as the main pathogens causing strawberry red–stele root rot. For both fungi, optimal mycelial growth occurred at 25 °C under a 12-h light/12-h dark photoperiod; the optimal pH values were 7.0 for <em>F. oxysporum</em> and 6.0 for <em>F. solani</em>. Optimal conidial production for <em>F. oxysporum</em> was observed at 25 °C, pH 7.0, and 24 h light, whereas <em>F. solani</em> sporulated best at 30 °C under a 12  h light/12  h dark cycle. <em>In vitro</em>, 300 million spores/g <em>Trichoderma harzianum</em> and 0.3 % matrine emulsifiable concentrate (EC) strongly inhibited pathogens, the EC₅₀ values of 300 million spores/g <em>Trichoderma harzianum</em> for <em>F. oxysporum</em> and <em>F. solani</em> are 6.42 ± 1.40 mg L⁻¹ and 8.58 ± 2.39 mg L⁻¹, respectively, while the EC₅₀ values of 0.3 % matrine for <em>F. oxysporum</em> and <em>F. solani</em> are 2.50 ± 1.31 mg L⁻¹ and 0.73 ± 0.21 mg L⁻¹, respectively.Field efficacy tests showed that 300 million spores/g <em>Trichoderma harzianum</em> and 0.3 % matrine EC provided the best control effect, both reducing disease incidence to 35.00 %, lowering the disease index to 27.67 and 26.33, and achieving a controlled efficacy of 63.59 and 65.36 %, These findings clarify the etiology of strawberry red–stele root rot in northern Zhejiang and offer practical guidance for its diagnosis and control.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105876"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-organic fertilizers containing potential biocontrol strains suppress bacterial soft rot and reshape soil microbial communities in cucumbers","authors":"Zhongqiang Gao ,&nbsp;Xiaoting Wang ,&nbsp;Xiwu Ding ,&nbsp;Xia Gao ,&nbsp;Yanan Han ,&nbsp;Biao Gong ,&nbsp;Jian Wang ,&nbsp;Weiqiang Li ,&nbsp;Fenghui Wu","doi":"10.1016/j.biocontrol.2025.105891","DOIUrl":"10.1016/j.biocontrol.2025.105891","url":null,"abstract":"<div><div>Bacterial soft rot disease poses a serious threat to cucumber production. Bio-organic fertilizers containing antagonistic beneficial microorganisms have emerged as promising approaches for enhancing plant disease resistance. However, the underlying mechanisms by which these fertilizers suppress bacterial soft rot disease remain unclear. This study aimed to explore the fundamental patterns of bio-organic fertilizer regulation of bacterial and fungal community assembly in cucumbers and their relationships with bacterial soft rot resistance through a field experiment involving four different types of bio-organic fertilizer treatments. The plant health and soil fertility increased significantly under all treatments. The combination of Chinese medicine residue with bio-organic fertilizer demonstrated the most pronounced effects among all treatments. Shifts in bacterial and fungal community structure induced by bio-organic fertilizers are crucial in suppressing bacterial soft rot disease. In particular, bio-organic fertilizers recruit more beneficial microorganisms with antimicrobial activity and promote plant growth traits. Following bio-organic fertilizer application, the bacterial network displays greater complexity than the fungal network. Structural equation models have demonstrated the influence of bio-organic fertilizer application on specific microflora that drives modifications in soil physicochemical properties. Altering key soil physicochemical factors such as total nitrogen and total phosphorus is vital for promoting the suppressive effect of bio-organic fertilizers on bacterial soft rot. Thus, the effectiveness of bio-organic fertilizers stems from a combination of the actual antagonistic activities of the inoculated biocontrol agents and the promotion of indigenous beneficial microbial groups. This dual mechanism not only suppresses the growth of pathogens directly but also strengthens the soil’s overall microbial ecosystem, thereby promoting plant health and resistance to diseases.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105891"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moth plant Araujia sericifera, an exotic smothering vine in New Zealand: Surveys for candidate biocontrol agents in South America, agent prioritization and safety testing","authors":"Quentin Paynter ,&nbsp;Freda E. Anderson ,&nbsp;Jane Barton ,&nbsp;María Virginia Bianchinotti ,&nbsp;Angela Bownes ,&nbsp;Diego Carpintero ,&nbsp;Soledad Delgado ,&nbsp;Rolf Delhey ,&nbsp;A.Hugh Gourlay ,&nbsp;Lynley Hayes ,&nbsp;Mirta Kiehr ,&nbsp;Zane McGrath ,&nbsp;Gustavo H. Ramírez ,&nbsp;Daniel Testoni ,&nbsp;C.B. Villamil ,&nbsp;Soledad C. Villamil","doi":"10.1016/j.biocontrol.2025.105901","DOIUrl":"10.1016/j.biocontrol.2025.105901","url":null,"abstract":"<div><div>Surveys in the native range of moth plant <em>Araujia sericifera</em> revealed a rich natural enemy biota attacking the plant, including damaging primary pathogens and root-boring and seed-feeding arthropod guilds, contrasting with surveys in New Zealand (NZ) that found only generalist defoliating and sucking arthropod herbivores and two mildly pathogenic fungi. A chrysomelid beetle with root boring larvae <em>Freudeita cupripennis</em>, a fruit fly <em>Anastrepha nigrotaenia,</em> and a defoliating rust fungus <em>Puccinia araujiae</em> were prioritised as potential biocontrol agents. Host-range testing indicated that all three species are sufficiently host-specific to be released in NZ. Approval was obtained to release an Argentinian population of <em>P. araujiae</em> and <em>F. cupripennis</em>, collected in Uruguay. Importation of <em>P. araujiae</em> into NZ was deferred until issues associated with the implementation of the Convention on Biological Diversity and associated development of Access and Benefit Sharing legislation in Argentina were overcome. It is hoped that this will proceed soon. <em>Freudeita cupripennis</em> was first released in 2019 and is established in NZ. An application to release <em>A. nigrotaenia</em> sourced from Uruguay was submitted in 2023. However, several other <em>Anastrepha</em> species are pests of fruit crops and some of NZ’s major fresh produce trading partners require declarations stating <em>Anastrepha</em> spp. are absent from NZ produce. This has delayed approval for the release of <em>A. nigrotaenia</em> as exporters will no longer be able to declare that the genus <em>Anastrepha</em> is absent from NZ if <em>A. nigrotaenia</em> is released to control moth plant. Consequently, trade agreements will require amendments to accommodate this.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105901"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and biocontrol of Planococcus citri Risso (Hemiptera: Pseudococcidae), a new pest of licorice (Glycyrrhiza uralensis Fisch.) using cuticle-degrading enzymes of Bacillus velezensis CE 100","authors":"Ju-Yeol Yun ,&nbsp;Jae-Hyun Moon ,&nbsp;Henry B. Ajuna ,&nbsp;Sang-Jae Won ,&nbsp;Choub Vantha ,&nbsp;Su-In Choi ,&nbsp;Su-Yeon Lee ,&nbsp;Iksoo Kim ,&nbsp;Tae-Joon Kim ,&nbsp;Young Sang Ahn","doi":"10.1016/j.biocontrol.2025.105904","DOIUrl":"10.1016/j.biocontrol.2025.105904","url":null,"abstract":"<div><div>Licorice is a valuable medicinal and industrial crop, but is highly prone to pest damage, especially to polyphagous sap-sucking mealybugs (Hemiptera) insect pests. This study reports for the first the identification citrus mealybugs as insect pests of tremendous economic importance in licorice production. The insect pests collected were identified as <em>Planococcus citri</em> based on analysis of the morphological features and DNA barcode sequencing of mitochondrial Cytochrome Oxidase subunit I (<em>COI</em>) gene. Due to the hazardous effects of chemical insecticides, this study investigated the entomopathogenic potential <em>Bacillus velezensis</em> CE 100 against <em>P. citri</em>. The bacterium produced cuticle-degrading enzymes: lipase, chitinase and protease in a pattern corresponding with cell growth. The maximum lipase, chitinase and protease activity was 32.7 unit/mL, 96.5 unit/mL and 35.7 unit/mL, respectively, which corresponded to the highest cell growth of 25.6 × 10⁷ colony-forming units, observed after 6 days of incubation. Treatment of <em>P. citri</em> with bacterial culture caused the hydrolysis of the cuticular wax layer, chitin laminae and structural proteins of <em>P. citri</em>, resulting in 80.0 % mortality within 6 h after treatment. The control and PB media treatment did not cause insect mortality. The scanning electron microscopy analysis of the treated insects revealed severe cuticle degradation, with deep cracks extending from the epicuticle to the procuticle, and the erosion of the wax layer and broken setae. This study identified <em>P. citri</em> as an insect pest of economic importance in licorice cultivation, for the first time in the world. It also demonstrates the biopesticides potential of <em>B. velezensis</em> CE 100 against <em>P. citri,</em> through the lipolytic, chitinolytic and proteolytic activities, which could enhance licorice production and quality.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105904"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol effect and mechanism of endophytic fungus Alternaria alternata against root rot of Lycium barbarum","authors":"Bin Wang ,&nbsp;Caixia Hou ,&nbsp;Wei Chen ,&nbsp;Yuyan Sun ,&nbsp;Xia Wu ,&nbsp;Chongqing Zhang ,&nbsp;Jing He","doi":"10.1016/j.biocontrol.2025.105900","DOIUrl":"10.1016/j.biocontrol.2025.105900","url":null,"abstract":"<div><div>Root rot caused by <em>Fusarium solani</em> is a severe soil-borne disease affecting <em>Lycium barbarum</em> (goji berry), which has long been managed through chemical control. However, prolonged reliance on chemical agents can lead to pathogen resistance and environmental risks. Initially isolated from healthy <em>L. barbarum</em>, the strain YBG8 exhibited antagonistic properties against <em>F. solani</em> and was identified as <em>Alternaria alternata</em>. However, the precise antifungal mechanism of YBG8 remained unclear. This study aimed to elucidate the antifungal mechanism and biocontrol effect of the fermentation filtrate of the antagonistic strain YBG8. The findings indicated that the YBG8 fermentation filtrate treatment significantly impeded the radial growth of <em>F. solani</em>, altered its spore surface morphology, and triggered an upsurge in alkaline phosphatase activity. Additionally, YBG8 fermentation filtrate treatment also resulted in heightened levels of malondialdehyde (MDA), increased electrical conductivity, and notable leakage of intracellular proteins and nucleic acids. Assessment with propidium iodide staining validated the disruption of cell membrane integrity. Liquid chromatography-mass spectrometry (LC-MS) analysis of the YBG8 fermentation filtrate unveiled 252 metabolites, encompassing coumarins, carboxylic acid derivatives, purine nucleosides, and others, with 15 constituents exhibiting concentrations exceeding 10 μg/mL. Subsequent analysis identified alternariol methyl ether as the predominant polyketide compound, with a concentration of 130.086 μg/mL. Additionally, altenusin, a diphenyl ether derivative, and altenuene, a cell wall synthesis inhibitor, were also detected at concentrations of 26.664 μg/mL and 18.291 μg/mL, respectively. These findings collectively suggest that the YBG8 fermentation filtrate inhibits the growth of <em>F. solani</em> through multiple pathways, such as cell wall disruption, oxidative stress induction, and membrane function interference. Alternariol methyl ether, altenusin, and altenuene are presumed to be the primary antimicrobial agents in the fermentation filtrate. This investigation illustrates that the YBG8 fermentation filtrate exerts potent antimicrobial effects through a dual-targeting strategy that disrupts both cell wall and membrane systems. These findings establish a theoretical framework and material basis for the development of environmentally friendly biopesticides.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105900"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus velezensis SBB80 affects the development of Fusarium and Neocosmospora species and controls Diatraea saccharalis (Lepidoptera: Crambidae) larvae","authors":"Marcelo Tavares de Castro ,&nbsp;Antônia Débora Camila de Lima Ferreira ,&nbsp;Izabela Nunes do Nascimento ,&nbsp;Gabriela Teodoro Rocha ,&nbsp;Ítalo Alves Freire ,&nbsp;Giovana Cidade Gomes ,&nbsp;Breno Beda dos Reis Cunha ,&nbsp;Sandro Coelho Linhares Montalvão ,&nbsp;Flávia Melo Moreira ,&nbsp;Rosiane Andrade da Costa ,&nbsp;Rose Gomes Monnerat","doi":"10.1016/j.biocontrol.2025.105888","DOIUrl":"10.1016/j.biocontrol.2025.105888","url":null,"abstract":"<div><div><em>Bacillus velezensis</em> is a species of gram-positive spore-forming bacteria that has gained prominence in agricultural use, especially to control fungi and phytopathogenic nematodes. This work aimed to: i) carry out the morphological, molecular, biochemical and physiological characterization of a Brazilian <em>B. velezensis</em> strain (SBB80); ii) evaluate its antagonistic potential against eight isolates of <em>Fusarium</em> spp. and two of <em>Neocosmospora</em> spp.; iii) and determine its toxic effect on larvae of six insect pests from the order Lepidoptera. As a result, the SBB80 strain was able to synthesize siderophores, antimicrobial lipopeptides as surfactin and fengycin, exopolysaccharides at 28 °C, and the enzymes protease, lipase, cellulase, catalase and phosphatase, inhibiting the growth of the eight <em>Fusarium</em> isolates (51.01 % to 61.82 % inhibition), the two <em>Neocosmospora</em> isolates (57.84 % and 73.80 % inhibition), and control <em>Diatraea saccharalis</em> caterpillars (77.08 % mortality) after 7 days. These results show that the <em>B. velezensis</em> SBB80 strain has great potential to be used in the field for the management of pests and diseases and greenhouse and field studies must be conducted to validate its efficiency.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105888"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}