Biological Control最新文献

{"title":"Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana","authors":"Qikai Zhang ,&nbsp;Dengjie Wang ,&nbsp;Xinghui Liang ,&nbsp;Haihong Wang ,&nbsp;Baoqian Lyu","doi":"10.1016/j.biocontrol.2025.105824","DOIUrl":"10.1016/j.biocontrol.2025.105824","url":null,"abstract":"<div><div>The leafminer <em>Liriomyza sativae</em> Blanchard (Diptera: Agromyzidae) is a significant pest damaging various agricultural and ornamental crops. Control is hindered by its resistance to multiple insecticides. This study aimed to develop and optimize an autoinoculation device specifically for controlling <em>L. sativae</em> and to assess the device’s efficacy in field applications. The initial phase involved screening to identify a strain of <em>Beauveria bassiana</em> (Balsamo-Crivelli) Vuillemin that was highly virulent to <em>L. sativae</em>. After selection of the optimal strain, the attractant formulation was refined and the device design was optimized to maximize pest attraction and infection rates. Short- and long-term field trials were then conducted to evaluate the device’s effectiveness in the control of pest populations. Results showed that the XJWLMQ-1 strain of <em>B. bassiana</em> had the highest rates of spore production, pest infection, and lethality to the targeted leafminer. The most effective attractant was a blend of <em>trans</em>-2-hexenal, <em>cis</em>-3-hexenol, 1-octen-3-ol, <em>cis</em>-3-hexenyl acetate, and α-ionone in a 4:1:1:3:1 ratio at 0.8 g/L concentration. Optimal efficacy was achieved by positioning the attractant 20 cm from the fungal spores and 20 cm above the cowpea canopy. Both short- and long-term trials confirmed that the autoinoculation device was effective in controlling <em>L. sativae</em> populations, suggesting its effective use for biological control of the target pest.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105824"},"PeriodicalIF":3.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)","authors":"Aline Nunes-Silva ,&nbsp;Camila Costa Moreira ,&nbsp;Janaína Brandão Seibert ,&nbsp;Jonathan Rodríguez ,&nbsp;Italo Delalibera-Júnior","doi":"10.1016/j.biocontrol.2025.105823","DOIUrl":"10.1016/j.biocontrol.2025.105823","url":null,"abstract":"<div><div>Entomopathogenic fungi have been widely used for sustainable agriculture as biological pest control agents. However, commercially available isolates were not efficient in controlling the stink bug <em>Euschistus heros</em> (Hemiptera: Pentatomidae), one of the most destructive species in soybean cultivation. There is an increasing demand for exploring new microorganisms that have the potential to control this insect. The present study evaluated the susceptibility of <em>E. heros</em> to 15 isolates of different species of the genus <em>Metarhizium</em>. A mortality rate of over 95 % was observed in isolates of <em>M. pingshaense</em>, <em>M. humberi,</em> and <em>M. robertsii</em> in 8 days of experiment. The aerial conidia yield of ESALQ 4395 and ESALQ 3364 isolates stood out with a concentration above 1x10⁹ conidia/g of dry rice, which is similar to commercial isolates. The average lethal concentration of these isolates was 5.1x10⁵ conidia/mL for adult insects, with a 95 % confidence interval of 4.7x10⁵ to 5.4x10⁵ conidia/mL for ESALQ 4395 and 4.8x10⁵ to 5.5x10⁵ conidia/mL for ESALQ 3364. However, no age-dependent mortality was observed since mortality among adults was more pronounced than in immature insects. Although low efficiency of entomopathogenic fungi for controlling stink bugs has been associated with the fungistatic and fungicidal action of the volatile compounds present in the alarm pheromone, the data obtained here demonstrated that this insect is highly susceptible to <em>Metarhizium,</em> especially to species that are not explored commercially in Brazil such as <em>M. humberi, M. pinghaense</em> and <em>M. robertsii</em>.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105823"},"PeriodicalIF":3.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass release of susceptible males dilutes Bollgard II® Bt resistance in pink bollworm: A semi-field validated strategy","authors":"Suresh R. Jambagi ,&nbsp;M. Mohan ,&nbsp;K. Muralimohan ,&nbsp;T. Venkatesan ,&nbsp;Satya Nand Sushil","doi":"10.1016/j.biocontrol.2025.105822","DOIUrl":"10.1016/j.biocontrol.2025.105822","url":null,"abstract":"<div><div>The pink bollworm, <em>Pectinophora gossypiella</em> (Lepidoptera: Gelechiidae), poses a significant threat to Bt cotton cultivation in India due to field-level resistance against Cry1Ac and Cry2Ab toxins. Existing pest management tactics have proven inadequate. To address this, homozygous susceptible (RR) male moths were mass-reared in the laboratory and released into Bt Bollgard II® cotton fields. Success was enhanced by trapping and removing feral resistant males with pheromone traps prior to releasing susceptible males. The effectiveness was assessed using bioassays, resistance dilution genetics, fitness cost estimation, field validation, and gene expression analysis. Resistance dilution was evident in both laboratory back-cross progenies and field progenies from feral females mated with released susceptible males. Resistance was autosomal with recessive inheritance for both Cry toxins. In treated plots, boll damage and larval numbers were significantly reduced. Genes linked to Bt resistance, including <em>PgCad1</em>, <em>PgABCA2</em>, <em>APN</em>, and <em>ABCG8</em>, showed significant down regulation (−4.93, −2.63, −1.73, and −1.49 fold, respectively), indicating partial restoration of susceptibility in field-collected larvae following mating (Field-R ♀ × Lab-S ♂). These findings highlight the potential of mass-releasing susceptible males as an effective and sustainable strategy for managing Bt-resistant pink bollworm, providing an alternative to traditional insecticide-based approaches.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105822"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of a novel broad-spectrum antifungal strain Bacillus velezensis Shannan.BV80-12 isolated from the Tibetan Plateau","authors":"Yali Tan ,&nbsp;Feiyan Dong ,&nbsp;Rong Wang ,&nbsp;Xiao Li ,&nbsp;Jialan Zhang ,&nbsp;Tong Gu ,&nbsp;Li Li ,&nbsp;Mengxiang Gao","doi":"10.1016/j.biocontrol.2025.105821","DOIUrl":"10.1016/j.biocontrol.2025.105821","url":null,"abstract":"<div><div>Plant diseases caused by pathogenic fungi are major contributors to global crop loss. Although chemical fungicides are effective for disease control, concerns regarding their adverse effects on human health and the environment have driven interest in sustainable alternatives. Beneficial microorganism-based biological controls have attracted considerable attention in this context. In this study, a novel bacterial strain, <em>Bacillus velezensis</em> Shannan.BV80-12, was isolated from the high-altitude Tibetan Plateau. The strain demonstrated significant inhibitory effects against 21 plant pathogenic fungi across 12 genera, showing inhibition rates between 61.9% and 97.5% in dual-culture assays and between 40.2% and 89.0% in poison agar assays with 2% cell-free supernatant. It also effectively controlled fungal plant diseases in detached leaf assays. Genome sequencing and analysis revealed the genetic basis for its biocontrol efficacy, including 19 biosynthetic gene clusters responsible for secondary metabolites and 23 glycoside hydrolase genes involved in degrading phytopathogen cell walls. UPLC-MS analysis of cell-free supernatants identified six distinct families of antimicrobial metabolites: fengycins (C14–C17), surfactins (C12–C16), iturins (C14–C16), bacillibactin, plantazolicin and bacilysin, suggesting that strain Shannan.BV80-12 possesses the capability to co-produce a variety of bioactive metabolites that synergistically contribute to its strong antimicrobial effects. Genome annotation and comparative genomics further revealed multiple genes associated with bacteria-plant interactions that can enhance plant health and resistance to pathogens, as well as genes crucial for adaptation to harsh environments, which may contribute to the stability and efficacy of the strain in controlling phytopathogens under adverse conditions. These findings elucidate the biocontrol mechanisms of <em>B. velezensis</em> strain Shannan.BV80-12 and support its potential application as a broad-spectrum biocontrol agent against phytopathogenic fungi in sustainable agriculture.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105821"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycin and peptin lipopeptides are major contributors to the biocontrol of Sclerotinia sclerotiorum by Pseudomonas mediterranea","authors":"Antoine Zboralski ,&nbsp;Renée St-Onge ,&nbsp;Marie Ciotola ,&nbsp;Mélanie Cadieux ,&nbsp;Martin Filion","doi":"10.1016/j.biocontrol.2025.105808","DOIUrl":"10.1016/j.biocontrol.2025.105808","url":null,"abstract":"<div><div>The fungal pathogen <em>Sclerotinia sclerotiorum</em> is the causal agent of the white mold disease in several economically important crops. Control methods are currently limited to the use of synthetic fungicides and resistance to these fungicides is increasing. Biological control may prove to be a more sustainable control strategy for this disease, as well as other fungal diseases. In a previous study, our team identified <em>Pseudomonas</em> strains that can reduce white mold symptom severity in lettuce. The exact mechanisms underlying this biocontrol were not explored, but the biocontrol activity of these <em>Pseudomonas</em> strains strongly correlated with the presence of three gene clusters involved in the biosynthesis of the lipopeptides brabantamides, corpeptin, and thanamycin. In this study, to better understand the mechanisms at play and progress towards the development of a <em>Pseudomonas</em>-based biocontrol inoculant against <em>S. sclerotiorum</em>, key genes involved in the biosynthesis of these lipopeptides were knocked out in the genome of <em>P. mediterranea</em> B21-060. The impact that the loss of function had on the ability of <em>P. mediterranea</em> to inhibit the growth or <em>S. sclerotiorum in vitro</em> and control white mold development <em>in planta</em> was evaluated. Production of corpeptin and thanamycin by <em>P. mediterranea</em> was required to repress the growth of <em>S. sclerotiorum</em> and suppress white mold symptom development in lettuce. Furthermore, corpeptin and thanamycin were also identified as key inhibitory molecules against <em>Botrytis cinerea</em> and <em>Rhizoctonia solani</em>, two other important fungal pathogens. This study highlights the potential of corpeptin and thanamycin in <em>Pseudomonas</em>-mediated control of different fungal plant diseases.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105808"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation and mechanisms of Bacillus velezensis AX22 against rice bacterial blight","authors":"Xin Shan,&nbsp;Jiawei Dai,&nbsp;Zhaofeng Xu,&nbsp;Yuting Diao,&nbsp;Ning Yang,&nbsp;Yongjie Fan,&nbsp;Meiqi Ma,&nbsp;Jiahui Zhao,&nbsp;Xiang Li,&nbsp;Shenghan Gao,&nbsp;Ming Xiao,&nbsp;Junmin Pei","doi":"10.1016/j.biocontrol.2025.105820","DOIUrl":"10.1016/j.biocontrol.2025.105820","url":null,"abstract":"<div><div>Rice bacterial blight (BB), caused by <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> (<em>Xoo</em>) poses a serious threat to global rice production, leading to substantial yield losses. Although biocontrol strategies utilizing antagonistic microorganisms have shown potential, systematic evaluations of their comprehensive efficacy and underlying mechanisms remain limited. In this study, a promising biocontrol agent, AX22, was isolated from the rhizosphere soil of BB-infected rice and evaluated through a novel multi-index assessment framework that integrates direct antagonistic activity with indirect plant growth-promoting and systemic resistance-inducing traits, offering a more comprehensive evaluation compared to conventional single-trait or dual-trait screening methods. AX22 exhibited strong antagonistic activity, along with the ability to produce exopolysaccharides (EPS), siderophores (SID), indole-3-acetic acid (IAA), and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Pot experiments confirmed that AX22 significantly enhanced rice disease resistance, reducing the BB index by 63.53 %, promoting plant growth, and inducing systemic resistance. Whole-genome sequencing identified AX22 as <em>Bacillus velezensis</em> and revealed multiple biocontrol-related genes, particularly a key difficidin biosynthetic gene cluster. Antibacterial assays showed that AX22 cell-free supernatant and purified difficidin effectively disrupted <em>Xoo</em> cellular morphology, resulting in nucleic acid and protein leakage and suppressing pathogenic traits such as biofilm formation, EPS production, and extracellular enzyme activity. These findings establish AX22 as a potent biocontrol agent against <em>Xoo</em>, with difficidin playing a critical role in its antagonistic effects. This study presents a multi-parameter evaluation framework for biocontrol agents and provides a foundation for their application in sustainable rice bacterial blight management.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105820"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-associated cues and their effects on the jumping behavior of Steinernema siamkayai","authors":"Puping Ta-oun ,&nbsp;Toyoshi Yoshiga","doi":"10.1016/j.biocontrol.2025.105804","DOIUrl":"10.1016/j.biocontrol.2025.105804","url":null,"abstract":"<div><div>Entomopathogenic nematodes are insect parasites with infective juveniles (IJs) employing host-seeking behaviors from ambush to cruise foraging. In ambush-foraging species, IJs often exhibit jumping behavior for attachment to host insects. <em>Steinernema siamkayai</em>, an ambush forager, exhibits both standing and jumping behaviors. However, the factors that enhance this behavior<!--> <!-->in <em>S. siamkayai</em> and how these factors differ among ambush foragers remain poorly understood. We investigated the influence of insect-associated cues on the jumping behavior of the Thai strain of <em>S. siamkayai</em> compared with <em>S. carpocapsae.</em> In the absence of stimuli, both species jumped 5.5 ± 1.9 mm and 3.8 ± 0.7 mm, respectively, with no observable effect of stimuli on jump distance. However, insect odors and CO₂ triggered directional jumping toward the source, while the proportion of jumps toward insect odors decreased when CO₂ was removed for both species. A higher percentage of <em>S. siamkayai</em> responded to insect odors by jumping compared with <em>S. carpocapsae</em>. Notably, air movement insignificantly affected <em>S. siamkayai</em> jumping, unlike in <em>S. carpocapsae</em>. An increase in stable standing behavior was observed in <em>S. siamkayai</em> populations when the absence of host cues. Furthermore, when stimulated by insect odors and CO₂, <em>S. siamkayai</em> jumped twice as quickly as when stimulus was absent. These findings suggest that <em>S. siamkayai</em> is more sensitive to insect-related odors and behaves as an active ambush forager. This high responsiveness appears to be a key behavioral trait for infecting insect pests that actively move near the soil surface in tropical regions.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105804"},"PeriodicalIF":3.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting Trichoderma antagonism: Role of strain identity, volatiles, biomass, and morphology in suppressing cacao pathogens","authors":"Insuck Baek ,&nbsp;Jishnu Bhatt ,&nbsp;Jae Hee Jang ,&nbsp;Seunghyun Lim ,&nbsp;Amelia Lovelace ,&nbsp;Minhyeok Cha ,&nbsp;Dilip Lakshman ,&nbsp;Moon S. Kim ,&nbsp;Lyndel W. Meinhardt ,&nbsp;Sunchung Park ,&nbsp;Ezekiel Ahn","doi":"10.1016/j.biocontrol.2025.105807","DOIUrl":"10.1016/j.biocontrol.2025.105807","url":null,"abstract":"<div><div>Cacao farming worldwide suffers from damaging fungal infections, including those caused by <em>Colletotrichum gloeosporioides</em> and <em>Pestalotiopsis</em> spp., which prioritizes the development of sustainable control solutions. This study evaluated the <em>in vitro</em> antagonistic potential of three <em>Trichoderma</em> strains (<em>T. virens</em> 11C-65-1, <em>T. virens</em> 29-8, <em>Trichoderma</em> spp. RC) against six cacao pathogen isolates from Ghana. Dual culture assays, detailed morphological analysis of both antagonist and pathogen, volatile organic compound (VOC) assays (comparing standard plug vs. pre-grown biomass methods), and the effect of UVC pretreatment were investigated. Multivariate statistics and machine learning were employed to analyze interaction dynamics and predict outcomes. All tested <em>Trichoderma</em> strains significantly inhibited pathogen growth, with efficacy varying notably: 11C-65-1 &gt; RC &gt; 29-8. Significant morphological changes were observed in both interacting fungi. Machine learning models predicted pathogen colony size with high accuracy (test set R² up to 0.94), identifying the specific <em>Trichoderma</em>-pathogen pair identity and <em>Trichoderma</em> circularity as the most crucial predictors. VOCs contributed to inhibition, and using a larger <em>Trichoderma</em> biomass drastically increased antagonistic effects, likely through combined VOC action and physical interaction. UVC pretreatment induced statistically significant but minimal morphological changes under the tested conditions. Multivariate analyses linked <em>Trichoderma</em> strain identity strongly with the resulting circularity of both antagonist and pathogen. Key takeaways from this research are the pronounced strain specificity governing <em>Trichoderma</em> antagonism, the indication of multiple active mechanisms, the relevance of morphology in these fungal battles, and the identification of <em>T. virens</em> 11C-65-1 as a strong prospect for biocontrol application against cacao pathogens, offering a data-driven approach for selecting effective biocontrol agents in other agricultural systems.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105807"},"PeriodicalIF":3.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aurantiaca ST-TJ4 against crown gall caused by Agrobacterium tumefaciens by inducing systemic resistance in cherry blossom","authors":"Qiao-Qiao Zhang ,&nbsp;Tong-Yue Wen ,&nbsp;Wei-Liang Kong ,&nbsp;Xiao-Qin Wu","doi":"10.1016/j.biocontrol.2025.105813","DOIUrl":"10.1016/j.biocontrol.2025.105813","url":null,"abstract":"<div><div>Cherry blossom crown gall has caused serious damage to plant growth, and is highly contagious and extremely difficult to control. The antagonism of pathogens by rhizosphere bacteria has attracted widespread attention. However, there is still limited research on the cherry blossom crown gall. In this study, we explored the control effect of rhizosphere bacteria <em>Pseudomonas aurantiaca</em> ST-TJ4 on cherry blossom crown gall. We also investigated the long-term survival status of ST-TJ4 in the cherry blossom roots and the induction of plant defense resistance. The results showed that ST-TJ4 had obvious inhibition effect on the population of <em>Agrobacterium tumefaciens</em>, which could reduce the number of <em>A. tumefaciens</em> by 70% to 90%, and its population kept the advantage in the rhizosphere soil and cherry blossom roots. The incidence of crown gall in the therapy group and the prevention group was reduced by 37.5% and 50%, respectively, and the disease index was reduced from 80 to 20 and 10, respectively. At the 150th day, ST-TJ4 could still be isolated from the rhizosphere soil and root surface, indicating that ST-TJ4 could survive in soil for a long time and had long-term performance. Compared with the control group, the therapy group and prevention group could reduce the levels of H₂O₂, malondialdehyde (MDA) and the oxidative damage, and up-regulated the expression of active oxygen-related genes <em>DHAR1</em>, <em>SOD1</em>, <em>GR1</em> and <em>CAT</em> to activate defense response. On the other hand, it could up-regulate the expression of <em>SA1</em>, <em>SA2</em> and <em>JA1</em> genes related to the induction of salicylic acid (SA) and jasmonic acid (JA), and lead to the increase of SA hormone level. Collectively, <em>P. aurantiaca</em> ST-TJ4 had the potential to be applied for biocontrol of cherry blossom crown gall by reducing root pathogen colonization and inducing plant resistance.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105813"},"PeriodicalIF":3.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in planta testing of microbial agents for dual biological control of granary weevil and storage fungi on stored wheat grain","authors":"Gülçin Ercan ,&nbsp;Anthony O. Adesemoye ,&nbsp;Gary Y. Yuen ,&nbsp;Sydney Everhart ,&nbsp;James F. Campbell ,&nbsp;Julie A. Peterson","doi":"10.1016/j.biocontrol.2025.105812","DOIUrl":"10.1016/j.biocontrol.2025.105812","url":null,"abstract":"<div><div>The granary weevil <em>Sitophilus granarius</em> is a serious pest that causes large economic losses to stored cereals. Similarly, storage fungi in the genera <em>Aspergillus, Fusarium</em>, and <em>Penicillium</em> can infest stored grain, causing deterioration of grain quality and contamination with mycotoxins that threaten animal and human health. The impact of these pests is magnified when they co-occur. Current management includes chemical pesticides and the manipulation of storage conditions, but undesirable pesticide residues, evolution of resistance, and practical and economic limitations may reduce their effectiveness and adoption. The aim of this study was to identify biological control agents (BCAs) that show dual effects against both granary weevil and three important storage fungi in stored grain. <em>In vitro</em> and <em>in planta</em> bioassays tested the effects of five bacterial and ten fungal strains of BCAs on granary weevil and three storage fungi: <em>Aspergillus parasiticus</em>, <em>Fusarium graminearum</em>, and <em>Penicillium chrysogenum</em>. The <em>in vitro</em> bioassays challenged storage fungi or weevils directly with BCAs in Petri plates while the <em>in planta</em> bioassays subjected pests to wheat seeds treated with BCAs. All BCAs exhibited some inhibitory effects against all pests, although <em>Trichoderma gamsii</em> (strains E1032 and E1064) and <em>Bacillus amyloliquefaciens</em> C415 were the most effective in causing mortality of granary weevil and suppressing growth of storage fungi. <em>Metarhizium anisopliae</em> E213 and <em>Bacillus thuringiensis</em> C423 showed strong sublethal effects on granary weevil by reducing oviposition and feeding damage. These studies reveal the potential for dual biological control of critical insect and storage fungi pests in stored cereal grains.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105812"},"PeriodicalIF":3.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}