金假单胞菌ST-TJ4诱导抗农杆菌引起的樱花冠瘿病

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2025-06-02 DOI:10.1016/j.biocontrol.2025.105813

Qiao-Qiao Zhang , Tong-Yue Wen , Wei-Liang Kong , Xiao-Qin Wu

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引用次数: 0

摘要

樱花冠瘿对植株生长造成严重危害，具有高度传染性，极难控制。根际细菌对病原菌的拮抗作用引起了广泛的关注。然而，对樱花冠瘿的研究仍然有限。本研究探讨了根际细菌金假单胞菌ST-TJ4对樱花花冠瘿病的防治作用。我们还研究了ST-TJ4在樱花根部的长期存活状况及其对植物防御抗性的诱导作用。结果表明，ST-TJ4对农杆菌种群有明显的抑制作用，可使农杆菌种群数量减少70% ~ 90%，且其种群在根际土壤和樱花根部保持优势。治疗组和预防组的冠瘿发生率分别降低了37.5%和50%，疾病指数分别从80降低到20和10。在第150天，ST-TJ4仍能从根际土壤和根表面分离出来，说明ST-TJ4能在土壤中存活较长时间，具有长期的性能。与对照组相比，治疗组和预防组可降低H2O2、丙二醛（MDA）水平，降低氧化损伤，上调活性氧相关基因DHAR1、SOD1、GR1、CAT的表达，激活防御反应。另一方面，上调与水杨酸（SA）和茉莉酸（JA）诱导相关的SA1、SA2和JA1基因的表达，导致SA激素水平升高。综上所述，P. aurantiaca ST-TJ4具有减少病原菌定植和诱导植株抗性的生物防治潜力。

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Pseudomonas aurantiaca ST-TJ4 against crown gall caused by Agrobacterium tumefaciens by inducing systemic resistance in cherry blossom

Cherry blossom crown gall has caused serious damage to plant growth, and is highly contagious and extremely difficult to control. The antagonism of pathogens by rhizosphere bacteria has attracted widespread attention. However, there is still limited research on the cherry blossom crown gall. In this study, we explored the control effect of rhizosphere bacteria Pseudomonas aurantiaca ST-TJ4 on cherry blossom crown gall. We also investigated the long-term survival status of ST-TJ4 in the cherry blossom roots and the induction of plant defense resistance. The results showed that ST-TJ4 had obvious inhibition effect on the population of Agrobacterium tumefaciens, which could reduce the number of A. tumefaciens by 70% to 90%, and its population kept the advantage in the rhizosphere soil and cherry blossom roots. The incidence of crown gall in the therapy group and the prevention group was reduced by 37.5% and 50%, respectively, and the disease index was reduced from 80 to 20 and 10, respectively. At the 150th day, ST-TJ4 could still be isolated from the rhizosphere soil and root surface, indicating that ST-TJ4 could survive in soil for a long time and had long-term performance. Compared with the control group, the therapy group and prevention group could reduce the levels of H₂O₂, malondialdehyde (MDA) and the oxidative damage, and up-regulated the expression of active oxygen-related genes DHAR1, SOD1, GR1 and CAT to activate defense response. On the other hand, it could up-regulate the expression of SA1, SA2 and JA1 genes related to the induction of salicylic acid (SA) and jasmonic acid (JA), and lead to the increase of SA hormone level. Collectively, P. aurantiaca ST-TJ4 had the potential to be applied for biocontrol of cherry blossom crown gall by reducing root pathogen colonization and inducing plant resistance.

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.