Antoine Zboralski , Renée St-Onge , Marie Ciotola , Mélanie Cadieux , Martin Filion
{"title":"Mycin and peptin lipopeptides are major contributors to the biocontrol of Sclerotinia sclerotiorum by Pseudomonas mediterranea","authors":"Antoine Zboralski , Renée St-Onge , Marie Ciotola , Mélanie Cadieux , Martin Filion","doi":"10.1016/j.biocontrol.2025.105808","DOIUrl":null,"url":null,"abstract":"<div><div>The fungal pathogen <em>Sclerotinia sclerotiorum</em> is the causal agent of the white mold disease in several economically important crops. Control methods are currently limited to the use of synthetic fungicides and resistance to these fungicides is increasing. Biological control may prove to be a more sustainable control strategy for this disease, as well as other fungal diseases. In a previous study, our team identified <em>Pseudomonas</em> strains that can reduce white mold symptom severity in lettuce. The exact mechanisms underlying this biocontrol were not explored, but the biocontrol activity of these <em>Pseudomonas</em> strains strongly correlated with the presence of three gene clusters involved in the biosynthesis of the lipopeptides brabantamides, corpeptin, and thanamycin. In this study, to better understand the mechanisms at play and progress towards the development of a <em>Pseudomonas</em>-based biocontrol inoculant against <em>S. sclerotiorum</em>, key genes involved in the biosynthesis of these lipopeptides were knocked out in the genome of <em>P. mediterranea</em> B21-060. The impact that the loss of function had on the ability of <em>P. mediterranea</em> to inhibit the growth or <em>S. sclerotiorum in vitro</em> and control white mold development <em>in planta</em> was evaluated. Production of corpeptin and thanamycin by <em>P. mediterranea</em> was required to repress the growth of <em>S. sclerotiorum</em> and suppress white mold symptom development in lettuce. Furthermore, corpeptin and thanamycin were also identified as key inhibitory molecules against <em>Botrytis cinerea</em> and <em>Rhizoctonia solani</em>, two other important fungal pathogens. This study highlights the potential of corpeptin and thanamycin in <em>Pseudomonas</em>-mediated control of different fungal plant diseases.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"207 ","pages":"Article 105808"},"PeriodicalIF":3.7000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Control","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1049964425001185","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The fungal pathogen Sclerotinia sclerotiorum is the causal agent of the white mold disease in several economically important crops. Control methods are currently limited to the use of synthetic fungicides and resistance to these fungicides is increasing. Biological control may prove to be a more sustainable control strategy for this disease, as well as other fungal diseases. In a previous study, our team identified Pseudomonas strains that can reduce white mold symptom severity in lettuce. The exact mechanisms underlying this biocontrol were not explored, but the biocontrol activity of these Pseudomonas strains strongly correlated with the presence of three gene clusters involved in the biosynthesis of the lipopeptides brabantamides, corpeptin, and thanamycin. In this study, to better understand the mechanisms at play and progress towards the development of a Pseudomonas-based biocontrol inoculant against S. sclerotiorum, key genes involved in the biosynthesis of these lipopeptides were knocked out in the genome of P. mediterranea B21-060. The impact that the loss of function had on the ability of P. mediterranea to inhibit the growth or S. sclerotiorum in vitro and control white mold development in planta was evaluated. Production of corpeptin and thanamycin by P. mediterranea was required to repress the growth of S. sclerotiorum and suppress white mold symptom development in lettuce. Furthermore, corpeptin and thanamycin were also identified as key inhibitory molecules against Botrytis cinerea and Rhizoctonia solani, two other important fungal pathogens. This study highlights the potential of corpeptin and thanamycin in Pseudomonas-mediated control of different fungal plant diseases.
期刊介绍:
Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents.
The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.