Biomedical reports最新文献

{"title":"Clinical application of the HALP score for predicting early neurological deterioration in older patients with acute cerebral infarction.","authors":"Yong-Juan Lin, Ai-Bin Guo, Ming-Min Huang, Xue Liang, Yu Xie, Ling-Ling Li","doi":"10.3892/br.2025.2097","DOIUrl":"10.3892/br.2025.2097","url":null,"abstract":"<p><p>The present study aimed to examine the relationship between the composite biomarker hemoglobin, albumin, lymphocyte and platelet (HALP) score and early neurological deterioration (END) to identify patients predisposed to poor neurological outcomes. A total of 148 older patients with acute ischemic stroke (AIS) who developed END and 283 without END were included. END was defined as an increase of at least two points in the National Institutes of Health Stroke Scale (NIHSS) score within 7 days. Multivariate logistic regression was performed to assess predictors of the HALP score. Receiver Operating Characteristic (ROC) curves and calibration plots were employed to evaluate model performance. The results demonstrated that END occurred in 34.34% of participants. Univariate analysis demonstrated significant differences between the two groups in age, baseline NIHSS score, white blood cell count, lymphocyte count, HALP score, levels of hemoglobin, triglycerides, C-reactive protein, homocysteine, lipoprotein phospholipase A2, and the incidence of infections and mortality (all P<0.05). In multivariate logistic regression, both the HALP score [odds ratio (OR): 0.965; 95% confidence interval (CI): 0.943-0.988; P=0.003) and baseline NIHSS score (OR: 1.169; 95% CI: 1.119-1.220; P<0.001) emerged as independent predictors of END. The areas under the ROC curve for a HALP score <27.69 and NIHSS score >5.5 were 0.727 (95% CI: 0.676-0.778) and 0.868 (95% CI: 0.834-0.903), respectively, with a combined AUC of 0.883 (95% CI: 0.850-0.916). The HALP score demonstrated a significant negative correlation with baseline NIHSS (r=-0.411, P<0.001) and NIHSS at day 7 (r=-0.348, P<0.001). Patients with lower HALP scores exhibited higher rates of END and poorer 90-day functional outcomes. In conclusion, reduced HALP scores were independently associated with END in older patients with AIS, suggesting that HALP may serve as an effective biomarker for the early identification of individuals at elevated risk of unfavorable neurological progression.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"24 2","pages":"24"},"PeriodicalIF":2.5,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145802848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial properties of Maillard reaction products: Molecular mechanisms and influencing factors (Review).","authors":"Zhongshuang Hu, Jiao Li, Xianghuan Wu, Yongzhong Wei, Xudong Li, Jing Ji","doi":"10.3892/br.2025.2019","DOIUrl":"10.3892/br.2025.2019","url":null,"abstract":"<p><p>The Maillard reaction, a non-enzymatic process involving the interaction between carbonyl and amino groups, has attracted substantial interest in biomedical research due to the antibacterial properties of its products. Maillard reaction products (MRPs) exhibit broad-spectrum activity against both foodborne bacteria and pathogenic strains, including antibiotic-resistant strains. These compounds inhibit microbial growth, regulate virulence and disrupt biofilm formation through mechanisms such as metal chelation, membrane disruption and interference with bacterial metabolism. As a result, MRPs emerge as promising alternatives to conventional antibiotics. In the biomedical field, MRPs hold potential for applications in medical device coatings and gut microbiota modulation, where they can prevent microbial colonization and influence host-microbe interactions. However, concerns regarding their safety, cytotoxicity and structural complexity pose significant challenges for broader implementation. Future research should focus on elucidating the structure-activity relationships of MRPs, optimizing their production and evaluating their long-term effects in biological systems. The present review provides an in-depth examination of the antibacterial mechanisms of MRPs, with a particular focus on identifying the bioactive molecules responsible for their activity. Additionally, it explores the potential biomedical applications of MRPs as sustainable antimicrobial agents.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"23 3","pages":"141"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of immunostimulation on autoinflammatory disease. CRMO case presentation and literature review: A case report.","authors":"Andrei-Ioan Munteanu, Delia-Maria Nicoară, Iulius Jugănaru, Meda-Ada Bugi, Otilia Mărginean","doi":"10.3892/br.2025.1993","DOIUrl":"10.3892/br.2025.1993","url":null,"abstract":"<p><p>Chronic recurrent multifocal osteomyelitis (CRMO) is a rare bone condition included in the spectrum of autoinflammatory pediatric rheumatological diseases. In the present case report, a 9-year-old female patient with a personal history of psoriasis since the age of 5 presented with left clavicle pain persisting for ~2 months. Following thorough anamnesis and investigations carried out, a diagnosis of CRMO was established. The present case report discusses a rare pediatric autoinflammatory pathology with autoimmune implications, emphasizing the role of immunostimulation/immune dysfunction. It is possible that immunostimulation with <i>Propionibacterium acnes</i> (also known as <i>Cutibacterium acnes</i>) for psoriasis management may underlie the development of autoinflammatory-destructive bone lesions. Establishing causal relationships between immunological, bacterial and autoinflammatory factors was challenging during the patient's evaluation in our service.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"23 1","pages":"115"},"PeriodicalIF":2.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Both asparagine and proline are required to decrease <i>Sestrin2</i> mRNA levels via ATF4 reduction and regulate collagen type I alpha 1 chain production and the proliferation of quiescent RI?T hepatic stellate cells.","authors":"Ran Sawa, Manami Ogawa, Hana Suzuki, Yasuhiko Okimura","doi":"10.3892/br.2025.1971","DOIUrl":"10.3892/br.2025.1971","url":null,"abstract":"<p><p>Sestrin 2 (SESN2) is a conserved protein whose expression is upregulated under various cellular stresses including hepatic injury. In the injured liver, hepatic stellate cells (HSCs) become activated and produce collagen, contributing to fibrosis; however, SESN2 overexpression has been shown to suppress collagen synthesis. Amino acids are known to influence SESN2 expression; however, their specific effects remain unclear. In the present study, it was investigated whether specific amino acids regulate SESN2 expression and the function of quiescent RI-T HSCs, which are responsible for collagen production, using reverse transcription-quantitative PCR, western blotting, and cell proliferation assay. It was found that supplementation with asparagine (Asn) and proline (Pro) (AP), both non-essential amino acids, led to a complete reduction in <i>SESN2</i> and <i>activating transcription factor 4 (ATF4)</i> mRNA levels after 5 h of incubation. Additionally, AP partially reduced <i>collagen type I α1 (COL1A1)</i> mRNA levels. However, knockdown of SESN2 or ATF4 resulted in a more substantial reduction in <i>COL1A1</i> mRNA levels than the supplementation with AP. These results suggest that SESN2, which is induced by amino acid insufficiency, contributes to the upregulation of <i>COL1A1</i> mRNA levels and that AP may increase <i>COL1A1</i> mRNA levels through pathways independent of SESN2. The COL1A1-inducing effect of SESN2 contrasted with the inhibitory effect of SESN2 on activated HSCs. In long-term cultures, AP supplementation increased <i>COL1A1</i> mRNA and protein levels, as well as RI-T cell proliferation, while <i>SESN2</i> and <i>ATF4</i> mRNA levels remained suppressed. These findings suggested that the absence of AP induces relative amino acid starvation, leading to increased ATF4/SESN2 expression. By contrast, long-term AP supplementation alleviated this stress, promoting cell proliferation and COL1A1 synthesis. The present results indicate that SESN2 function in quiescent HSCs may differ from its role in activated cells, providing new insights into its regulatory mechanisms in collagen production.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"22 6","pages":"93"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early detection of cardiac impairment and prediction of right ventricular hypertrophy in patients with connective tissue disease.","authors":"Jiayan Shen, Guangrong Xiao, Shuqing Fang, Haohao Yang, Zheren Zhao, Yun Xu, Meng Jiang, Song Zhong","doi":"10.3892/br.2025.1970","DOIUrl":"https://doi.org/10.3892/br.2025.1970","url":null,"abstract":"<p><p>Progressive right ventricle (RV) failure and death in connective tissue disease (CTD) are related to RV hypertrophy (RVH) and dilation, irrespective of pulmonary arterial hypertension (PAH). Therefore, detecting cardiac impairment before RVH and determining RVH predictors is crucial for timely intervention. The present prospective cohort study aimed to identify cardiac markers that occur before RVH and to investigate predictors of RVH. CTD was diagnosed based on clinical features, laboratory findings and imaging data. The cardiac functions of patients with CTD were evaluated using echocardiography, cardiovascular magnetic resonance (CMR) and multi-modality cardiac imaging studies, including RV wall thickness, systolic functions, late gadolinium enhancement, T1 maps and biventricular strain analysis. A total of 52 patients with CTD with non-right ventricular hypertrophy (non-RVH), 34 patients with RVH and 50 healthy individuals were prospectively included. The impaired cardiac indices in patients with RVH included RV ejection fraction, ventricular dimensions, global myocardial deformation, late gadolinium enhancement and ventricular extracellular volume (ECV). The cardiac death rate did not differ significantly between the RVH and non-RVH groups (P=0.14). Conventional parameters, including serum cardiac markers and the left ventricular ejection fraction, showed no significant changes in the non-RVH group compared with the control group. Regarding fibrosis assessment using CMR, an elevated native T1 value (1,362±72 msec in the non-RVH group vs. 1,268±42 in the control group; P<0.001) and ECV (31±4% in the non-RVH group vs. 25±3% in the control group; P<0.001) were observed. By contrast, T1 myocardium/msec 15 min post-contrast of the left ventricle in the RVH group was significantly decreased compared with that in the non-RVH group, indicating an increase in the extracellular matrix at this stage. RVH was predicted by pulmonary arterial pressure (PAP) in patients in the non-RVH group (t-statistic, 2.84; P=0.01), whereas after RVH presentation, RV end-systolic volume (RVESV) became a progression predictor of RVH (t-statistic, 7.98; P<0.0001). No other cardiac imaging or laboratory findings predicted RVH. To the best of our knowledge, the present study was the first to highlight the non-invasive detection of cardiac tissue impairment using CMR and provide support for cardiac treatment initiation before RVH detection. The predictors of RVH vary with the heart disease stage. PAP in the non-RVH stage and incompetence of RVESV in the RVH stage predicted the progression of RVH. The present study was part of a clinical trial (NCT03271385), which was registered on July 1, 2017, and started on September 1, 2017.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"22 5","pages":"92"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLE1 corepressor promotes gefitinib resistance in lung cancer A549 cells via E‑cadherin silencing.","authors":"Xin Yao, Nasir Roberts, Prince Iheukwumere, Alana Carmouche, Renwei Chen, Ma Carmela Dela Cruz, Hector Biliran","doi":"10.3892/br.2024.1914","DOIUrl":"10.3892/br.2024.1914","url":null,"abstract":"<p><p>As a putative lung specific oncogene, the transducin-like enhancer of split 1 (TLE1) corepressor drives an anti-apoptotic and pro-epithelial-mesenchymal transition (EMT) gene transcriptional programs in human lung adenocarcinoma (LUAD) cells, thereby promoting anoikis resistance and tumor aggressiveness. Through its survival- and EMT-promoting gene regulatory programs, TLE1 may impact drug sensitivity and resistance in lung cancer cells. In the present study, a novel function of TLE1 was uncovered as an inhibitor of the antitumor effects of the epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) gefitinib in the human LUAD cell line A549, which exhibits moderate sensitivity to EGFR-TKI. While upregulation of TLE1 expression potently inhibited the proliferation inhibitory and apoptotic effects of gefitinib in A549 cells, downregulation of endogenous TLE1 in these cells enhanced their sensitivity to gefitinib. In experimentally derived gefitinib-resistant A549 cells (A549GR) that have acquired EMT, TLE1 expression is upregulated as compared with parental A549 cells, and acute ablation of TLE1 expression is sufficient to partially restore gefitinib sensitivity and attenuate EMT phenotype. Mechanistic studies showed that TLE1 confers gefitinib resistance in A549 cells in part via downregulation of E-cadherin, a known potentiator of EGFR-TKI sensitivity and apoptosis induction. Importantly, the TLE1/E-cadherin transcriptional axis is negatively regulated by gefitinib to trigger apoptosis via the Bcl-2-inhibitor of transcription 1 cell death pathway. In conclusion, these results indicate a novel role of TLE1 in modulating EGFR-TKI sensitivity in lung cancer cells via regulation of E-cadherin expression, and its upregulation may potentiate EGFR-TKI resistance in LUAD.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"22 3","pages":"36"},"PeriodicalIF":1.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rifaximin prophylaxis in MASLD‑hepatocellular carcinoma: Lessons from a negative animal model.","authors":"Larisse Longo, Gabriel Tayguara Silveira Guerreiro, Luiza Behrens, Matheus Henrique Mariano Pereira, Carlos Eduardo Pinzon, Carlos Thadeu Schmidt Cerski, Carolina Uribe-Cruz, Mário Reis Álvares-da-Silva","doi":"10.3892/br.2024.1882","DOIUrl":"10.3892/br.2024.1882","url":null,"abstract":"<p><p>The incidence of hepatocellular carcinoma (HCC) has been rising, particularly among individuals diagnosed with metabolic dysfunction-associated steatotic liver disease. In the present study, the prophylactic effects of rifaximin (RIF) on HCC, inflammatory markers and cardiovascular risk (CVR) were investigated in an animal model. Adult Sprague-Dawley rats were randomly allocated into three groups (n=10, each): Control [standard diet/water plus gavage with vehicle (Veh)], HCC [high-fat choline deficient diet (HFCD)/diethylnitrosamine (DEN) in drinking water/Veh gavage] and RIF [HFCD/DEN/RIF (50 mg/kg/day) gavage] groups. After euthanasia at week 16, biochemical/inflammatory markers and the liver histology were assessed. The results demonstrated that the HCC and RIF animals had a significant increase in fresh liver weight, liver weight/body weight ratio, serum total cholesterol (TC), high-density lipoprotein-cholesterol, triglycerides, hepatic lipid accumulation and hepatic concentration of triglycerides and TC, relative to the controls (P<0.001, for all). Additionally, the HCC and RIF animals had higher plasminogen activator inhibitor, intercellular adhesion molecule-1, E-selectin and CVR scores than the controls (P<0.001, for all). The HCC animals had higher interleukin (IL)-1β (P=0.011), IL-10 (P<0.001), toll-like receptor-2 (P=0.012), lipopolysaccharide-binding protein (P=0.018) and metalloproteinase-2 (P=0.003) levels than the RIF animals. Furthermore, liver steatosis, inflammation and fibrosis, along with increased collagen fiber deposition occurred in the HCC and RIF groups. However, HCC occurred only in 2 RIF rats. In conclusion, although most animals did not develop HCC in the present study, RIF positively affected liver inflammation markers involved in steatohepatitis pathogenesis.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"22 1","pages":"4"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active fraction of ground cherry (<i>Physalis angulata</i> L.) calyces attenuates azoxymethane dextran sulfate sodium‑induced colon carcinogenesis in mice.","authors":"Yanet Ocampo, Daneiva Caro, David Rivera, Jenny Castro, Indira Pájaro, Rubén Salas, Luis Franco","doi":"10.3892/br.2024.1876","DOIUrl":"10.3892/br.2024.1876","url":null,"abstract":"<p><p><i>Physalis angulata</i> L., commonly known as wild tomato or ground cherry, is widely used in tropical and subtropical areas to treat health disorders including inflammation, hepatitis, dermatitis, cancer and diabetes. In Colombia, anti-cancer and anti-inflammatory activity are the most common ethnopharmacological applications of <i>P. angulata</i> calyces. <i>P. angulata</i> dichloromethane fraction (PADF) has significant anti-inflammatory activity. The present study assessed the pharmacological effect of PADF on colorectal cancer (CRC) using cancer and normal human cells and an azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model. MTT and clonogenic assay, cell cycle and apoptosis analysis and mitochondrial membrane potential measurement were employed to evaluate <i>in vitro</i> activity of PADF. PADF selectively induced a cytotoxic effect against CRC cells via apoptosis and G2/M arrest. In the AOM/DSS model, treatment with PADF diminished tumor number and size, affected area and expression of proliferating cell nuclear antigen and promoted colon tissue repair. These effects might be related to the increased expression of p38 pro-apoptotic protein in addition to anti-inflammatory activity of PADF demonstrated by decreased levels of TNF-α, IL-6, and IL-1β. PADF may serve as a potential treatment for CRC. Further investigation is warranted to identify the bioactive components in PADF.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"21 6","pages":"188"},"PeriodicalIF":2.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11484217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of HSV‑1 shedding as a predictor of cerebral vasospasm severity in patients with subarachnoid hemorrhage.","authors":"Melanie Walker, Emma Federico, Joseph R Zunt, Michael R Levitt, Christine M Johnston","doi":"10.3892/br.2024.1865","DOIUrl":"10.3892/br.2024.1865","url":null,"abstract":"<p><p>Cerebral vasospasm (CV) is a critical determinant of outcomes in patients with aneurysmal subarachnoid hemorrhage (aSAH). Despite advances in neurocritical care, modifiable risk factors for CV remain poorly understood, and identifying them could significantly enhance patient management and treatment strategies. The present study explored the potential link between the reactivation of herpes simplex virus type 1 (HSV-1), a common resident virus in cranial nerves, and CV severity. It was hypothesized that higher HSV-1 viral load in saliva may be associated with increased CV severity. Saliva samples were collected on days 4, 7, 10 and 14 post-aSAH, and HSV-1 DNA levels were measured using quantitative PCR. CV severity was assessed using the Lindegaard ratio (LR), with an LR >3 considered the diagnostic threshold for CV. A total of 36 patients were enrolled, and 139 saliva samples were collected. HSV-1 DNA was detected in 19.4% of samples (27/139), and 44% of patients (16/36) developed CV. HSV-1 seropositive patients made up 88.9% (32/36) of the cohort, with 50% exhibiting viral shedding during the study period. None of the HSV-1 seronegative patients (11.1%, 4/36) exhibited viral shedding or developed CV. Regression analysis showed a positive association between HSV-1 viral load and CV severity, with viral load explaining 27.8% of the variability (P=0.005). Age was also significant, with older patients experiencing less severe CV (P<0.001). Supervised machine learning identified viral load thresholds that aligned with standard LR values for moderate and severe CV. While the small sample size and observational design limit the generalizability of the results, these findings suggested that earlier detection and intervention for CV could be informed by assessing HSV-1 serostatus and monitoring viral activity through saliva samples or other non-invasive methods, highlighting the need for larger, controlled studies to validate these results.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"21 6","pages":"177"},"PeriodicalIF":1.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallein increases prostaglandin F2α‑induced osteoprotegerin and IL‑6 secretion in osteoblasts.","authors":"Gen Kuroyanagi, Tomoyuki Hioki, Rie Matsushima-Nishiwaki, Takuya Omura, Osamu Kozawa, Haruhiko Tokuda","doi":"10.3892/br.2024.1835","DOIUrl":"10.3892/br.2024.1835","url":null,"abstract":"<p><p>Gallein is a known Gβγ subunit inhibitor, but its function in bone metabolism, especially in osteoblasts, and its molecular mechanism remains to be elucidated. Osteoprotegerin (OPG), which is secreted from osteoblasts, binds to nuclear factor kB receptor activator (RANK) ligand (RANKL) as a decoy receptor, prevents RANKL-RANK binding, and inhibits bone resorption. IL-6 is not only a bone resorption factor but also as a bone metabolism regulator. Prostaglandin F2α (PGF2α) promotes p44/p42 MAPK, p38 MAPK and stress-activated protein kinase/JNK phosphorylation in osteoblast-like MC3T3-E1 cells. In MC3T3-E1 cells, activated p44/p42 and p38 MAPKs promote IL-6 secretion and activated p44/p42 and p38 MAPKs and JNK promote OPG secretion. The present study aimed to investigate the effect and mechanism of gallein on PGF2α-induced OPG and IL-6 secretion using an osteoblastic MC3T3-E1 cell line. It was found that gallein significantly increased PGF2α-induced OPG and IL-6 secretion in the MC3T3-E1 cell. By contrast, fluorescein, which is a gallein-like compound that does not bind to Gβγ, did not affect PGF2α-induced OPG and IL-6 secretion. Gallein significantly improved the PGF2α-induced OPG and IL-6 mRNA expression levels. Gallein did not affect the PGF2α-activated phosphorylation of p44/p42 and p38 MAPKs and JNK. Gallein increased PGF2α-induced OPG and IL-6 secretion in osteoblasts, indicating that gallein may regulate bone remodeling via OPG/IL-6 in bone metabolism.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"21 5","pages":"147"},"PeriodicalIF":2.3,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}