Medical laser application : international journal for laser treatment and research最新文献

{"title":"Congresses 2011/2012","authors":"","doi":"10.1016/j.mla.2011.08.009","DOIUrl":"https://doi.org/10.1016/j.mla.2011.08.009","url":null,"abstract":"","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 4","pages":"Page 190"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.08.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138383473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"532 nm laser treatment of molluscum contagiosum","authors":"Gergely Klinda,&nbsp;Peter Urban,&nbsp;Carsten M. Philipp,&nbsp;H.-Peter Berlien","doi":"10.1016/j.mla.2011.08.005","DOIUrl":"10.1016/j.mla.2011.08.005","url":null,"abstract":"<div><h3>Objective</h3><p>This retrospective study was undertaken to examine the efficiency of a 532<!--> <!-->nm laser device in the treatment of molluscum contagiosum.</p></div><div><h3>Material and methods</h3><p>The medical records were analyzed of 32 patients with molluscum contagiosum who had been treated in our clinic with a 532<!--> <!-->nm laser (IDAS; Quantel Derma GmbH) in the time period 09/2005 to 04/2011.</p></div><div><h3>Results</h3><p>Of all the patients treated, a total of 84% required only one treatment, 13% required two and only one patient (3%) needed three laser sessions. The treatment was well-tolerated and only a few of the patients developed side effects, such as hypopigmentation and atrophic skin texture changes.</p></div><div><h3>Conclusion</h3><p>The efficiency of the 532<!--> <!-->nm laser is comparable with the dye laser, but the 532<!--> <!-->nm laser is lower in price and more commonly available than the dye laser. We recommend further prospective studies about the 532<!--> <!-->nm laser in the treatment of molluscum contagiosum.</p></div><div><h3>Ziel</h3><p>In dieser retrospektiven Studie wurde die Effektivität eines 532<!--> <!-->nm-Lasers (IDAS; Quantel Derma GmbH) in der Behandlung von Dellwarzen (Molluscum contagiosum) untersucht.</p></div><div><h3>Material und Methoden</h3><p>Es wurden die Behandlungsdokumentationen von 32 Patienten, die in unserer Klinik zwischen 09/2005 und 04/2011 wegen Molluscum contagiosum behandelt wurden, retrospektiv analysiert.</p></div><div><h3>Ergebnisse</h3><p>84% der Patienten benötigte eine Behandlung, 13% zwei und ein Patient (3%) drei Behandlungen. Die Behandlung wurde gut toleriert, die Komplikationsrate war gering. Die Komplikationen bestanden in Hypopigmentierungen und atrophischen Hautveränderungen.</p></div><div><h3>Schlussfolgerung</h3><p>Die Effektivität des 532<!--> <!-->nm-Lasers ist vergleichbar mit der des Farbstofflasers. Der 532<!--> <!-->nm-Laser ist weiter verbreitet und preiswerter als der Farbstofflaser. Wir empfehlen weitere prospektive Studien zur Beurteilung des Stellenwertes des 532<!--> <!-->nm-Lasers bei der Behandlung von Molluscum contagiosum.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 4","pages":"Pages 172-175"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.08.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wide-field, high-resolution two-photon tissue mapping of human skin ex vivo","authors":"Daniela Göppner ,&nbsp;Norma Mechow ,&nbsp;Julia Liebscher ,&nbsp;Erik Thiel ,&nbsp;Gunter Seewald ,&nbsp;Harald Gollnick ,&nbsp;Carsten M. Philipp ,&nbsp;Karl-Heinz Schönborn","doi":"10.1016/j.mla.2011.08.003","DOIUrl":"10.1016/j.mla.2011.08.003","url":null,"abstract":"<div><h3>Background and objectives</h3><p>Two-photon microscopy (TPM) generates high definition images from biological samples such as human skin in a non-invasive way. The focus of an ultrashort pulsed laser is scanned through the tissue and the fluorescence photons induced by two-photon excitation of endogeneous fluorophores are collected, measured and displayed as a scanning image. So far, one of the main disadvantages of TPM is the limitation of the scanning area to tenths of a millimeter by the field of view of the focusing optics. Essential pieces of information, such as the differentiation between benign or malign changes in biological tissue, are therefore not captured. Within a BMBF-supported research project “FluoTOM”, a new modified TPM scanning technique was developed to overcome these shortcomings, and pilot tests have been made on benign and malignant samples of human skin <em>ex vivo</em>.</p></div><div><h3>Material and methods</h3><p>The field of view of the new TPM was extended from the current standard of &lt;500<!--> <!-->μm to &gt;5<!--> <!-->mm. The spectral band option allows the synchronous recording of one to four images with exact local congruence, using photons from a choice of different spectral windows. These images are finally merged into a false-color presentation for interpretation. The spectral wavelengths can be chosen in the range of 350–650<!--> <!-->nm according to a specific diagnostic question. Real-time photography is used as an adjunctive method to locate the scan position with respect to the sample to be examined. Samples under investigation were unstained tissue slides or full paraffin-embedded biopsy/tissue blocks. The images obtained by our new method were subsequently compared with standard H&amp;E histology.</p></div><div><h3>Results</h3><p>Vertical laser scan images of superior quality from unstained histological slides as well as from the paraffin blocks of different benign and malignant cutaneous samples were made. When directly compared to the routine H&amp;E histology, which is the gold standard, relevant tissue structures and changes in the normal architecture could be clearly identified and confirmed respectively.</p></div><div><h3>Conclusion</h3><p>A new non-invasive TPM scanning technique has been established that provides high-resolution imaging of biological tissue, such as human skin, in a distortion-free way and with a diagnostic relevant imaging size, thus allowing the assessment of tissue-significant medical issues in greater detail. Current use of our new method <em>ex vivo</em> shows that it is particularly appropriate for diagnosis and research applications of human skin.</p></div><div><h3>Hintergrund</h3><p>Zwei-Photonen-Mikroskopie (2PM) erzeugt bekanntlich nicht-invasiv hochaufgelöste Bilder aus dem Volumen von biologischem Gewebe. Der Fokus eines ultrakurz gepulsten Lasers führt hierbei zur sogenannten Zweiphotonenanregung endogener Fluorophore, deren Emissionen gesammelt, gemess","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 4","pages":"Pages 158-165"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.08.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Announcement of Deutsche Gesellschaft für Lasermedizin (DGLM) e.V.","authors":"","doi":"10.1016/j.mla.2011.09.001","DOIUrl":"https://doi.org/10.1016/j.mla.2011.09.001","url":null,"abstract":"","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 4","pages":"Page 151"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138418630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of dermal water content determination by spatially resolved reflectance","authors":"Stefan Andree ,&nbsp;Henrike Wilms ,&nbsp;Jürgen Helfmann","doi":"10.1016/j.mla.2011.05.003","DOIUrl":"10.1016/j.mla.2011.05.003","url":null,"abstract":"<div><h3>Background</h3><p>The skin protects the body against environmental stress, for example contact with biological or chemical substances. The protection must be effective for substances soluble in both polar solvents such as water, and non-polar solvents such as oil. Therefore a specific balance of water and lipids contained in the skin is necessary to maintain the protection against both of these chemicals. Potential applications of assessing the water concentration in skin range from determining the effect of soap-like substances on the water balance in the skin over the efficiency of hydrating cosmetics to detecting water deposition in skin due to heart or kidney diseases.</p></div><div><h3>Objective</h3><p>To our knowledge, there is currently no accepted <em>in vivo</em> method for quantitative determination of the water content in skin. Our aim was therefore to apply the spatially resolved reflectance (SRR) method to assess <em>in vivo</em> the water concentration in the skin.</p></div><div><h3>Materials and methods</h3><p><span>An overview on the advantages and disadvantages of our set-up is presented compared to other SRR set-ups and other (invasive) methods to assess the water concentration in skin. The set-up is described and the procedure for the evaluation of the absorption coefficient, from which the water concentration is determined. The performance of our set-up was examined by using fluid phantoms containing </span>dimethyl sulfoxide (DMSO), water and scattering particles at different concentrations.</p></div><div><h3>Results</h3><p>The determined water concentration correlates linearly to the expected concentration with a correlation coefficient <em>R</em>² of 0.977. The derived water concentration, taken from the spatially and spectrally resolved skin measurements presented here, is comparable to the concentration expected from the literature and found to be a realistic figure of 80%.</p></div><div><h3>Conclusion</h3><p>The optical non-invasive determination of water concentration in the skin is feasible using SRR.</p></div><div><h3>Hintergrund</h3><p>Die Haut schützt den Körper vor Stress durch Umwelteinflüsse, zum Beispiel durch Kontakt mit biologischen oder chemischen Substanzen. Der Schutz muss sowohl für fett- als auch wasserlösliche Substanzen funktionieren. Daher ist es notwendig, dass sich eine Balance zwischen Wasser und Lipiden in der Haut ausbildet, um gegen beide Arten von Substanzen zu schützen.</p><p>Die Bestimmung der Wasserkonzentration der Haut spielt daher eine besondere Rolle, wenn beispielsweise Effekte von Seifen oder ähnlichen Substanzen auf den Wasserhaushalt der Haut oder die Effizienz feuchtigkeitsspendender Kosmetika untersucht oder aber Wassereinlagerungen verursacht durch Herz- oder Nierenkrankheiten bestimmt werden sollen.</p></div><div><h3>Zielsetzung</h3><p>Zurzeit sind uns keine Verfahren für die nicht-invasive, quantitative <em>In-vivo</em>-Messung des Wassergehaltes bekannt, die ","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 3","pages":"Pages 109-118"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reply to Prof. Bäumler's letter to the editor entitled “Powerful medical light sources such as lasers or IPL in the hands of laypersons?”","authors":"H.-Peter Berlien","doi":"10.1016/j.mla.2011.05.007","DOIUrl":"10.1016/j.mla.2011.05.007","url":null,"abstract":"","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 3","pages":"Pages 145-146"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.05.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy","authors":"Marieke Pudlas ,&nbsp;Daniel Alejandro Carvajal Berrio ,&nbsp;Miriam Votteler ,&nbsp;Steffen Koch ,&nbsp;Sibylle Thude ,&nbsp;Heike Walles ,&nbsp;Katja Schenke-Layland","doi":"10.1016/j.mla.2011.05.004","DOIUrl":"10.1016/j.mla.2011.05.004","url":null,"abstract":"<div><h3>Background</h3><p>Human bone marrow-derived mesenchymal stem cells (BM-MSCs) are a promising cell source for regenerative medical applications because they can be easily isolated and expanded <em>ex vivo</em>. However, due to the lack of specific cell surface markers, it is difficult to identify whether <em>ex vivo</em> isolated BM-MSC populations are free of stromal fibroblasts. Bright-field microscopical analyses are insufficient to determine fibroblastic contaminations since these two cell types have similar cell morphologies.</p></div><div><h3>Materials and methods</h3><p>We employed and compared traditional flow cytometric (FACS) analysis, <em>in vitro</em> differentiation assays, and Raman spectroscopy to distinguish between human BM-MSCs and fibroblasts.</p></div><div><h3>Results</h3><p>We found that FACS analysis, utilizing previously described fibroblast-identifying antibodies, was inadequate in separating stromal fibroblasts from BM-MSCs as over 75% of the BM-MSCs shared these antigens. <em>In vitro</em> differentiation assays revealed that, in contrast to fibroblasts, BM-MSCs could be successfully differentiated into adipocytes, osteoblasts and chondrocytes. Using this method it was possible to discriminate between the two cell types. However, the need for prolonged <em>in vitro</em> culture periods of up to 4 weeks is a major disadvantage of this test method. Raman spectroscopy, a non-contact technique measuring the wavelength and intensity of inelastic scattered light from molecules by employing high-power near-infrared lasers, distinguished ultra-fast between BM-MSCs and fibroblasts (integration time of 100<!--> <!-->s/cell).</p></div><div><h3>Conclusion</h3><p>Based on the results, we conclude that Raman spectroscopy is a suitable tool for the rapid detection of fibroblastic contaminations in BM-MSC cultures.</p></div><div><h3>Hintergrund</h3><p>Aus dem Knochenmark gewonnene mesenchymale Stammzellen (BM-MSCs) sind eine vielversprechende Zellquelle für Anwendungen innerhalb der regenerativen Medizin. Es ist jedoch schwierig zu bestimmen, ob isolierte BM-MSC-Populationen frei von Fibroblasten oder anderen, die Stammzellen kontaminierenden Zellen sind. Ziel der vorliegenden Studie war es festzustellen, ob sich die Raman-Spektroskopie zur nicht-invasiven, kontaktfreien Unterscheidung humaner BM-MSCs und Fibroblasten eignet.</p></div><div><h3>Material und Methoden</h3><p>Es wurden verschiedene Verfahren (Durchflusszytometrie, <em>In-vitro</em>-Differenzierungsassays, Raman-Spektroskopie) hinsichtlich der Differenzierbarkeit humaner BM-MSCs und Fibroblasten untersucht und verglichen.</p></div><div><h3>Ergebnisse</h3><p>Unsere Daten zeigen, dass Hellfeld-mikroskopische Analysen allein nicht ausreichen, um Fremdzellkontaminationen zu bestimmen, da BM-MSCs und Fibroblasten sehr ähnliche Zellmorphologien aufweisen. Auch die traditionelle durchflusszytometrische (FACS)-Analyse unter Verwendung zuvor beschriebener zell-populationsspez","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 3","pages":"Pages 119-125"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.05.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calibration standards and phantoms for fluorescence optical measurements","authors":"Uwe J. Netz,&nbsp;Jan Toelsner,&nbsp;Uwe Bindig","doi":"10.1016/j.mla.2011.05.002","DOIUrl":"10.1016/j.mla.2011.05.002","url":null,"abstract":"<div><p>We report on the development and production of tissue-like optical phantoms for detection and characterization of fluorescent objects close to the tissue surface. The phantoms mimic bulk optical properties such as light scattering and absorption of female breast tissue and human skin. Fluorescence sources are embedded for a given well-defined localization and volume to simulate pathologic lesions that are marked by specific molecular substances labeled with a fluorescent dye. The phantoms were made of epoxy resin with added titanium dioxide and black epoxy color paste for adjustment of the tissue-like scattering and absorbing properties. Indocyanine green was used as a fluorescent dye. The phantoms were evaluated with an experimental set-up for a new method for <em>in vivo</em> laser fluorescence tomography in the frequency domain.</p></div><div><p>Wir berichten in diesem Artikel über die Entwicklung und Herstellung von Phantomen mit gewebeähnlichen optischen Eigenschaften für die Detektion und Charakterisierung von oberflächennahen Fluoreszenzquellen. Die Phantome simulieren die optischen Parameter bezügliche Lichtstreuung und –absorption von weiblichem Brustgewebe bzw. Hautgewebe. Zur Simulation von pathologischen Gewebearealen, die mit einem spezifischen molekularen Marker belegt sind, an den ein Fluoreszenzfarbstoff gekoppelt ist, wurden in die Phantome Fluoreszenzquellen mit definierter Position und Ausdehnung integriert. Die Phantome bestehen aus Epoxidharz sowie Titandioxid und einer schwarzen Epoxid-Farbpaste zum Einstellen der gewebeähnlichen Streu- und Absorptionseigenschaften. Als Fluoreszenzfarbstoff wurde Indocyaningrün verwendet. Die Phantome wurden mit einem Experimentalaufbau für ein neues Verfahren einer <em>In-vivo</em>-Laserfluoreszenztomografie in der Frequency-Domain evaluiert.</p></div>","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 3","pages":"Pages 101-108"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Content","authors":"","doi":"10.1016/S1615-1615(11)00040-8","DOIUrl":"https://doi.org/10.1016/S1615-1615(11)00040-8","url":null,"abstract":"","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 3","pages":"Page iii"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1615-1615(11)00040-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138361010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcellular co-localization of aluminum (III) phthalocyanine chloride tetrasulphonate with fluorescent markers in the human melanoma cell-line HT-144","authors":"Ivy Ndhundhuma ,&nbsp;Carmen Hauser ,&nbsp;Claudia Scalfi-Happ ,&nbsp;Angelika Rück ,&nbsp;Rudolf Steiner","doi":"10.1016/j.mla.2011.04.001","DOIUrl":"10.1016/j.mla.2011.04.001","url":null,"abstract":"<div><h3>Objective</h3><p>The exposure of a photosensitizer to visible light, preferably laser light in the red region, in the presence of molecular oxygen results in a photochemical reaction described as photodynamic therapy (PDT). Photodynamic therapy is increasingly used for the treatment of skin cancers. The subcellular localization of the photosensitizer has been shown to be a key factor in the outcome of PDT. Mitochondrial localized photosensitizers are able to induce apoptosis very rapidly. Lysosomal localized photosensitizers can elicit either a necrotic or an apoptotic response. The present work contributes to the classification of the subcellular targets and cell death pathway of PDT using hydrophilic aluminum (III) phthalocyanine chloride tetrasulfonate (AlPcS₄).</p></div><div><h3>Material and methods</h3><p>The localization of AlPcS₄ in metastatic human melanoma (HT-144) cells was studied by means of fluorescent probes (Rh-123, LysoTracker^® and Fluo-3) and a laser scanning microscope (LSM).</p></div><div><h3>Results</h3><p>The laser sources of the LSM induced a PDT effect with a redistribution of AlPcS₄ inside the entire cell, followed by the increased fluorescence of AlPcS₄ in the cytoplasm of HT-144 cells.</p></div><div><h3>Conclusion</h3><p>The results suggest that AlPcS₄ is a potential sensitizer for melanoma skin cancer treatments. However, a comparison of its cytotoxicity, uptake and accumulation in normal and cancer cells deserve further investigation.</p></div><div><h3>Hintergrund</h3><p>Die Bestrahlung eines Photosensibilisators mit sichtbarem Licht, vorzugsweise Laserlicht im roten Spektralbereich, und in Gegenwart von molekularem Sauerstoff führt zu einer photochemischen Reaktion, die als photodynamische Therapie (PDT) bezeichnet wird. Die PDT wird zunehmend für die Behandlung von Hauttumoren eingesetzt. Für den Erfolg der PDT ist die subzelluläre Lokalisation des Photosensibilisators ausschlaggebend. In Mitochondrien lokalisierte Photosensibilisatoren können beschleunigt Apoptose auslösen. In Lysosomen lokalisierte Photosensibilisatoren können entweder Nekrose oder Apoptose hervorrufen. Die vorliegende Arbeit trägt zur Klassifikation subzellulärer Zielstrukturen bei, die über entsprechende Signalwege zum Zelltod führen.</p></div><div><h3>Material und Methoden</h3><p>Die Lokalisation von hydrophilem, tetra-sulfoniertem Aluminium(III)-Phthalocyanin-Chlorid (AlPcS₄) in metastasierenden humanen Melanom-Zellen (HT-144) wurde mit Hilfe von Fluoreszenzmarkern (Rhodamin 123, LysoTracker^® und Fluo-3) und dem Laser-Scan-Mikroskop (LSM) untersucht.</p></div><div><h3>Ergebnisse</h3><p>Die Laserstrahlquellen im LSM erzeugten einen PDT-Effekt, der zu einer Umverteilung des AlPcS₄ in den Zellen führte, gefolgt von einem Fluoreszenzanstieg des AlPcS₄ im Zytoplasma der HT-144 Zellen.</p></div><div><h3>Zusammenfassung</h3><p>AlPcS<s","PeriodicalId":88584,"journal":{"name":"Medical laser application : international journal for laser treatment and research","volume":"26 3","pages":"Pages 93-100"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mla.2011.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54921066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}