Biologia futuraPub Date : 2025-09-01Epub Date: 2025-05-02DOI: 10.1007/s42977-025-00256-4
Krisztián Sepp, Péter Hausinger, Béla Hegedűs, Péter Sándor Kiss, Edina Cseszkó, Miklós Mózes, Marianna Radács, Zsuzsanna Valkusz, Márta Gálfi, Zsolt Molnár
{"title":"Effects of in vivo chlorobenzene exposure on bone tissue in a rat model.","authors":"Krisztián Sepp, Péter Hausinger, Béla Hegedűs, Péter Sándor Kiss, Edina Cseszkó, Miklós Mózes, Marianna Radács, Zsuzsanna Valkusz, Márta Gálfi, Zsolt Molnár","doi":"10.1007/s42977-025-00256-4","DOIUrl":"10.1007/s42977-025-00256-4","url":null,"abstract":"<p><p>Calcipaenic bone disorders (e.g., osteoporosis) are becoming an epidemic as a significant public health concern. The underlying genetic, epigenetic, and homeostatic factors and the determinants of bone tissue expression are triggered by environmental exposures. Endocrine disruptor compounds are important in the development of pathological bone alterations. The aim of this study is to design an in vivo subtoxic chlorobenzene exposure model that can be used to explore certain bone changes and their consequences. Male Wistar rats were treated via gastric tube with a 1:1 mixture of hexachlorobenzene + 1,2,4-trichlorobenzene at a dose of 1.0 μg/kg bw; in a final volume of 1 mL, for 30, 60 and 90 days. Blood serum and bone samples were obtained from the femur diaphysis. The results of the treatments (n = 10/group) were interpreted as related to the controls. Serum levels of γGT, SGOT, SGPT were determined, along with bone tissue morphology, as well as the total mineral content of the bone and the mobilizable anorganic content. ANOVA was used to analyze the measurement data. As a result of the treatment protocol, histological examinations of bone morphology showed osteoid degeneration, as well as an altered state of the bone matrix. These findings are supported by the DEXA images, which showed a time-dependent decrease in surface mineral content, in parallel, an increase in the mobilizable anorganic content of the bone was detected. These results suggest that chlorobenzene administered may be a causal factor and changes in bone tissue structure can be traced.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"359-370"},"PeriodicalIF":1.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-09-01Epub Date: 2025-06-06DOI: 10.1007/s42977-025-00261-7
Tian Zhang, Yang Liu, Junde Li, Mingjun Yuan, Chunhan Qiao, Xiao Huang, Xueyao Yang, Bo Gao, Chuan Lou, Yan Yang, Yu Cao
{"title":"Toad's survivability and soil microbiome alterations impacted via individual abundance.","authors":"Tian Zhang, Yang Liu, Junde Li, Mingjun Yuan, Chunhan Qiao, Xiao Huang, Xueyao Yang, Bo Gao, Chuan Lou, Yan Yang, Yu Cao","doi":"10.1007/s42977-025-00261-7","DOIUrl":"10.1007/s42977-025-00261-7","url":null,"abstract":"<p><p>Artificial breeding is a valid strategy for the reverse of current extinction tendency in wild population of amphibian like toads. Considering public health, an alternative to antibiotics is demanded for ameliorating survival of toads during the culture period. Relying on the cognition of probiotics or antagonistic bacteria, the present work investigated viability and soil microorganism variations induced by distribution characteristic on toads using high-throughput sequencing technology. Comparison and analysis of soil metagenome from clustered and depopulated groups distinguished by toad behavior showed differences of bacterial community composition (e.g., Proteobacteria bacterium TMED72 and Nannocystis exedens) and antibiotic resistance genes involving antibiotic efflux and inactivation (e.g., mdtB and acrF). There were 18 and 10 distribution-typical genes independently enriched in Proteobacteria bacterium TMED72 and bacterium TMED88 of clustered group and Nannocystis exedens of depopulated group. In Nannocystis exedens, one of the distribution-typical genes was annotated as 6-phosphogluconate dehydrogenase acting role on bacterial growth restriction. It implied that, compared with the group emerging rare traces, the reduction of soil bacteria which possess genes retarding bacterial growth putatively impairs competitiveness to pathogenic bacteria and results in poor survivability of toads under clustering behavior. With the co-occurrence of virulence genes, more evidences are needed on the antagonistic bacteria Nannocystis exedens as antibiotic substitute.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"399-411"},"PeriodicalIF":1.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-09-01Epub Date: 2025-06-17DOI: 10.1007/s42977-025-00259-1
Bendegúz Mihalik, Nóra Ágh, Ivett Pipoly, Edina Nemesházi, Krisztián Szabó, Gábor Seress, András Liker
{"title":"Low genetic differentiation and symmetric migration between urban and forest populations of great tits.","authors":"Bendegúz Mihalik, Nóra Ágh, Ivett Pipoly, Edina Nemesházi, Krisztián Szabó, Gábor Seress, András Liker","doi":"10.1007/s42977-025-00259-1","DOIUrl":"10.1007/s42977-025-00259-1","url":null,"abstract":"<p><p>Gene flow may be limited between urban and non-urban populations of wild animals that can influence their landscape-level genetic structure and potential to adapt to new ecological conditions. To test this idea, we genetically characterized great tit (Parus major) populations breeding in an urban and a forest area 3.5 km apart, differing in several phenotypic traits some of which may contribute to adaptation to urban living. We used 16 microsatellite markers to genotype 189 breeding adult individuals (119 urban and 70 forest birds) and (1) tested whether the two populations are genetically differentiated, and (2) estimated the rate and direction of migration between the sites. Heterozygosity tended to be lower in the urban than in the forest habitat. Genetic population structure analyses did not show a consistent clustering of breeding birds between the urban and forest sites, and this conclusion was not affected by the inclusion of phenotypic data in the analyses. The pairwise fixation index (F<sub>st</sub>) was low (0.009) and only 1% of the total genetic variance was explained by variation between populations. Finally, there was detectable gene flow between the two areas, and its estimated values did not suggest asymmetry in the direction of migration. We conclude that great tits living in the city are genetically connected to the nearby forest population by reciprocal migration, which may explain the low level of genetic differentiation.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"371-381"},"PeriodicalIF":1.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-09-01Epub Date: 2025-06-27DOI: 10.1007/s42977-025-00268-0
Marco d'Agostino, Emanuele Luigi Zenga, Manuela Giovanetti, Fortunato Fulvio Bitonto, Marta Galloni, Marino Quaranta, Laura Bortolotti
{"title":"A BEE indicator for monitoring wild bee diversity in agricultural systems.","authors":"Marco d'Agostino, Emanuele Luigi Zenga, Manuela Giovanetti, Fortunato Fulvio Bitonto, Marta Galloni, Marino Quaranta, Laura Bortolotti","doi":"10.1007/s42977-025-00268-0","DOIUrl":"10.1007/s42977-025-00268-0","url":null,"abstract":"<p><p>Given the growing importance of assessing the condition of wild bees in agroecosystems, we focused on developing a BEE indicator that could be easily used by non-expert taxonomists to score bee diversity. Our goal was to create a tool suitable for farm-level use, one that does not require taxonomic expertise nor heavy field work. Since existing literature has emphasized the significance of environmental features surrounding any investigated site, we incorporated this aspect into our design. We began by identifying a user-friendly field tool to differentiate among bee morphogenera, then proceeded by developing a process for data analyses and interpretation. A protocol is also shared. Further on, we run a case-study testing this tool at 13 sites in three countries, differentiating farms based on farming practices (conventional or organic, as proxy of opposite conditions). The results confirmed that (a) a diagnostic table based on morphological similarities is a practical field tool that, in almost all cases, allows for accurate classification of a bee individual into a specific group (morphogenus); (b) the process of analysis, based on number of morphogenera and landscape composition reflects variability among bees and across sites; (c) evaluating environmental features is essential for placing the results of bee variability into the appropriate context.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"437-449"},"PeriodicalIF":1.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Essential oils of Citrus limon, Cymbopogon citratus, and Lavandula officinalis disrupt E. coli biofilms by inducing cellular damage.","authors":"Aalia Khanem, Naheed Karim, Ikram Ullah, Farhan Younas","doi":"10.1007/s42977-025-00255-5","DOIUrl":"10.1007/s42977-025-00255-5","url":null,"abstract":"<p><p>Biofilms by E. coli is not only the primary cause of recurrent urinary tract infections, but also lead to medical device-associated infections. Ever increasing antibiotic resistance due to biofilms has sparked a search for plant-based replacements. This study examines anti-biofilm potential of essential oils from lemon (Citrus limon), lemongrass (Cymbopogon citratus), and lavender (Lavandula officinalis) against 26 clinical isolates of E. coli. Essential oils (EOs) were extracted by using hydro-distillation and characterized by GC-MS and FT-IR. Biofilms were quantitatively and qualitatively evaluated. The extracted essential oils had yields ranging from 1.37 to 1.45 (w/v). The main constituents were limonene (94%) in lemon, citral (42%) and ketones (9%) in lemongrass, and linalool (35%) and linalyl anthranilate (20%) in lavender EO. MICs and MBCs of EOs were in the range of 3.13-50 µg/ml. The oils under investigation exhibited notable biofilm inhibition and eradication properties. The percent inhibition and eradication varied between 6-89% and 3-82.25% (p < 0.0001), respectively. These findings suggest that essential oils from lemon, lemongrass, and lavender may offer a promising natural approach to combat E. coli biofilms and associated infections. Further research is warranted to elucidate the underlying mechanisms of action and to explore their clinical applications.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"343-358"},"PeriodicalIF":1.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-09-01Epub Date: 2025-04-17DOI: 10.1007/s42977-025-00254-6
Yue Li, Chuang Li, Zhongyi Xu, Yingsheng Liu, Shanshan Zhong, Zhelun Xu, Jun Liu, Congyan Wang, Daolin Du
{"title":"Soil bacterial community could be affected by Amaranthus retroflexus L.","authors":"Yue Li, Chuang Li, Zhongyi Xu, Yingsheng Liu, Shanshan Zhong, Zhelun Xu, Jun Liu, Congyan Wang, Daolin Du","doi":"10.1007/s42977-025-00254-6","DOIUrl":"10.1007/s42977-025-00254-6","url":null,"abstract":"<p><p>Invasive plants can disrupt the growth performance of native plants by releasing allelochemicals affecting on litter decomposition. Furthermore, these invaders can establish a plant-soil feedback loop with soil microorganisms, which promotes their continued successful invasion primarily through decomposition process. Consequently, it is of the utmost importance to conduct research that analyzes the impacts of invasive plants' allelopathy on their interaction with soil microorganisms. This study aims to investigate the effects of Amaranthus retroflexus L., an invasive Amaranthaceae plant's allelopathy, on its interaction with soil bacterial communities, compared to the native plant A. tricolor L., and also the impacts of the allelopathy of Amaranthus retroflexus on soil enzyme activities. The research was conducted via an indoor planting experiment in which a gradient of Amaranthus retroflexus leaf litter was added. In particular, Amaranthus retroflexus leaf litter resulted in an increase in pH, electrical conductivity, total nitrogen, and neutral protease activity in soil under certain treatments. The amount of Amaranthus retroflexus leaf litter and the form of incubation condition may be the primary determinants of the composition of bacterial communities in soil and the number of functional gene pathways of soil bacteria involved in the decomposition process (especially the decomposition of carbon-containing substances), rather than the alpha diversity of soil bacteria. Consequently, Amaranthus retroflexus may predominantly modify the composition of bacterial communities in soil and the number of functional gene pathways of soil bacteria involved in the decomposition process, rather than the alpha diversity of soil bacteria, to facilitate its subsequent invasion.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"329-341"},"PeriodicalIF":1.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-06-01Epub Date: 2025-07-08DOI: 10.1007/s42977-025-00272-4
Roszik Sára, Miklósi Ádám
{"title":"Reasons to avoid using weasel words in ethology: establishing a more technical terminology may facilitate synthesis in the behavioural sciences.","authors":"Roszik Sára, Miklósi Ádám","doi":"10.1007/s42977-025-00272-4","DOIUrl":"10.1007/s42977-025-00272-4","url":null,"abstract":"<p><p>The term 'weasel word' is used for vague, ambiguous, or misleading language when the speaker creates an impression of meaning or certainty without committing to specific facts. In academic writings, clear presentation of the declarant's thoughts is inevitable for the transmission and/or sharing of knowledge with high fidelity; hence, weasel words and phrases should be avoided. So far, the topic has received little attention in the behavioural sciences, including ethology, although research in fields focusing on behaviour and the mind provide fertile ground where such rhetorical strategies may go unnoticed. By presenting examples of weasel words used in ethology, we aim to demonstrate how they can lead to misunderstandings among scientists, as well as to prolonged and unproductive debates. We argue that the use of weasel words may stem equally from ingrained bad habits, knowledge gaps, anthropomorphism and inconsistent jargon. Instead of referring to mental states, the description of the behaviours should be in focus from a functional point of view. This allows more precise behaviour measurement and greater agreement in the use of terminology. Adopting a more descriptive and precise terminology may facilitate interdisciplinary discussions and lay the groundwork for novel approaches, such as synthetic or computational ethology.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"181-192"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-06-01Epub Date: 2025-07-08DOI: 10.1007/s42977-025-00275-1
D J Ujfalussy
{"title":"Do dogs understand?-Contrasting anecdotal reports, neuroimaging, and behavioural evidence on language comprehension.","authors":"D J Ujfalussy","doi":"10.1007/s42977-025-00275-1","DOIUrl":"10.1007/s42977-025-00275-1","url":null,"abstract":"<p><p>Dogs are the earliest domesticated species which still live alongside humans in unprecedented numbers. While various aspects of their socio-cognition have been shown to have adapted to the human environment during domestication, evidence on language comprehension is contradicting and inconclusive. In this review article, in an attempt to answer the hypothetical question: \"Do dogs understand human language?\" I try to summarize all recent behavioural, neurocognitive, as well as anecdotal reports we have on language comprehension in dogs. I assess the contradictions, suggest a set of possible explanations, and propose a new, systematic and more naturalistic approach to consolidate anecdotal and neuroimaging evidence with behavioural evidence.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"249-257"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-06-01Epub Date: 2025-08-11DOI: 10.1007/s42977-025-00278-y
Enikő Kubinyi, Ádám Miklósi
{"title":"Celebrating Professor Vilmos Csányi-Pioneer of Hungarian Ethology.","authors":"Enikő Kubinyi, Ádám Miklósi","doi":"10.1007/s42977-025-00278-y","DOIUrl":"10.1007/s42977-025-00278-y","url":null,"abstract":"","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"117-118"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biologia futuraPub Date : 2025-06-01Epub Date: 2025-08-11DOI: 10.1007/s42977-025-00279-x
Henrietta Bolló, Anna Kis, József Topál
{"title":"A functionalist approach to social status in social animals, humans and the dog-human community. The prestige-based social system hypothesis.","authors":"Henrietta Bolló, Anna Kis, József Topál","doi":"10.1007/s42977-025-00279-x","DOIUrl":"10.1007/s42977-025-00279-x","url":null,"abstract":"<p><p>Social status plays a crucial role in shaping interactions. Despite its broad significance, research on status hierarchies remains largely fragmented across disciplines and results relating to dominance in the field of biology versus results relating to prestige in the field of human sciences are not integrated in a unified framework. In this paper, we examine how dominance and prestige contribute to hierarchical structures, considering both functionalist and critical perspectives on social status, by reviewing empirical findings from primates, human societies, and the dog-human bond. While dominance is a common strategy for establishing status across species, here we argue that prestige-based hierarchies-characterized by social learning and cooperation-are particularly influential in both human and dog-human interactions. We propose extending the dominance-prestige model by introducing the concept of \"super-prestige\" which describes the dependency-driven relationship between dogs and humans. This perspective challenges traditional dominance-based frameworks and offers a novel approach to understanding interspecies social structures.</p>","PeriodicalId":8853,"journal":{"name":"Biologia futura","volume":" ","pages":"221-231"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}