Biochimica et biophysica acta. Molecular and cell biology of lipids最新文献

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TG-interacting factor 1 regulates mitotic clonal expansion during adipocyte differentiation TG 交互因子 1 在脂肪细胞分化过程中调控有丝分裂克隆扩增
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-04-02 DOI: 10.1016/j.bbalip.2024.159492
Yu-Hao Chang , Yu-Hua Tseng , Ju-Ming Wang , Yau-Sheng Tsai , Huei-Sheng Huang
{"title":"TG-interacting factor 1 regulates mitotic clonal expansion during adipocyte differentiation","authors":"Yu-Hao Chang ,&nbsp;Yu-Hua Tseng ,&nbsp;Ju-Ming Wang ,&nbsp;Yau-Sheng Tsai ,&nbsp;Huei-Sheng Huang","doi":"10.1016/j.bbalip.2024.159492","DOIUrl":"https://doi.org/10.1016/j.bbalip.2024.159492","url":null,"abstract":"<div><p>Obesity is one of the significant health challenges in the world and is highly associated with abnormal adipogenesis. TG-interacting factor 1 (TGIF1) is essential for differentiating murine adipocytes and human adipose tissue-derived stem cells. However, the mode of action needs to be better elucidated. To investigate the roles of TGIF1 in differentiation in-depth, CRISPR/Cas9 knockout technology was performed to generate TGIF1-silenced preadipocytes. The absence of TGIF1 in 3 T3-F442A preadipocytes abolished lipid accumulation throughout the differentiation using Oil Red O staining. Conversely, we established 3 T3-F442A preadipocytes stably expressing TGIF1 and doxycycline-inducible TGIF1 in TGIF1-silenced 3 T3-F442A preadipocytes. Remarkably, the induction of TGIF1 by doxycycline during the initial differentiation phase successfully promoted lipid accumulation in TGIF1-silenced 3 T3-F442A cells. We further explored the mechanisms of TGIF1 in early differentiation. We demonstrated that TGIF1 promoted the mitotic clonal expansion via upregulation of CCAAT/enhancer-binding proteins <em>β</em> expression, interruption with peroxisome proliferators activated receptor <em>γ</em> downstream regulation, and inhibition of p27<sup>kip1</sup> expression. In conclusion, we strengthen the pivotal roles of TGIF1 in early differentiation, which might contribute to resolving obesity-associated metabolic syndromes.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 5","pages":"Article 159492"},"PeriodicalIF":4.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140347191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tracing the lipidome in inborn errors of metabolism 追踪先天性代谢错误中的脂质体
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-03-31 DOI: 10.1016/j.bbalip.2024.159491
Martina Zandl-Lang
{"title":"Tracing the lipidome in inborn errors of metabolism","authors":"Martina Zandl-Lang","doi":"10.1016/j.bbalip.2024.159491","DOIUrl":"https://doi.org/10.1016/j.bbalip.2024.159491","url":null,"abstract":"<div><p>Inborn errors of metabolism (IEM) represent a heterogeneous group of more than 1800 rare disorders, many of which are causing significant childhood morbidity and mortality. More than 100 IEM are linked to dyslipidaemia, but yet our knowledge in connecting genetic information with lipidomic data is limited. Stable isotope tracing studies of the lipid metabolism (STL) provide insights on the dynamic of cellular lipid processes and could thereby facilitate the delineation of underlying metabolic (patho)mechanisms. This mini-review focuses on principles as well as technical limitations of STL and describes potential clinical applications by discussing recently published STL focusing on IEM.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 5","pages":"Article 159491"},"PeriodicalIF":4.8,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198124000416/pdfft?md5=08565a2d87213d62e0e1cd4bdf4bf8d7&pid=1-s2.0-S1388198124000416-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140347192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The unusual substrate specificity of Escherichia coli cardiolipin synthase C does not require the product of the transcriptionally engaged ymdB gene 大肠杆菌心磷脂合成酶 C 不同寻常的底物特异性并不需要转录参与的 ymdB 基因的产物。
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-03-26 DOI: 10.1016/j.bbalip.2024.159483
Katsuhiro Sawasato, Mikhail Bogdanov
{"title":"The unusual substrate specificity of Escherichia coli cardiolipin synthase C does not require the product of the transcriptionally engaged ymdB gene","authors":"Katsuhiro Sawasato,&nbsp;Mikhail Bogdanov","doi":"10.1016/j.bbalip.2024.159483","DOIUrl":"10.1016/j.bbalip.2024.159483","url":null,"abstract":"<div><p>Polycistronic transcription and translation of <em>ymdB</em>-<em>clsC</em> have been thought to be required for full activity of ClsC.</p><p>The authentic initiation codon of the <em>clsC</em> gene is present within the open reading frame of the upstream located <em>ymdB</em> gene.</p><p>ClsC translated from authentic initiation codon drives cardiolipin (CL) synthesis without transcriptionally paired YmdB.</p><p>YmdB is not necessary for the substrate specificity of ClsC utilizing phosphatidylethanolamine (PE) as a co-substrate.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 5","pages":"Article 159483"},"PeriodicalIF":4.8,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Imaging of perivascular adipose tissue in cardiometabolic diseases by Raman spectroscopy: Towards single-cell analysis 利用拉曼光谱对心脏代谢疾病中的血管周围脂肪组织进行成像:实现单细胞分析。
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-03-21 DOI: 10.1016/j.bbalip.2024.159484
Ewa Stanek , Krzysztof Czamara
{"title":"Imaging of perivascular adipose tissue in cardiometabolic diseases by Raman spectroscopy: Towards single-cell analysis","authors":"Ewa Stanek ,&nbsp;Krzysztof Czamara","doi":"10.1016/j.bbalip.2024.159484","DOIUrl":"10.1016/j.bbalip.2024.159484","url":null,"abstract":"<div><p>Perivascular adipose tissue (PVAT) has emerged as a dynamic organ influencing vascular function and cardiovascular health. In this brief review, an overview of the recent research in the investigation of PVAT is presented, ranging from in vivo studies to single-cell methodologies, in particular those based on Raman spectroscopy. The strengths and limitations of each, emphasizing their contributions to the current understanding of PVAT biology were discussed. Ultimately, the integration of these diverse methodologies promises to uncover new therapeutic targets and diagnostic biomarkers, including those emerging from simple Raman spectroscopy-based measurements of alterations in lipid unsaturation degree, invariably associated with PVAT dysfunction.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 5","pages":"Article 159484"},"PeriodicalIF":4.8,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SC5D is the sixth enzyme in cholesterol biosynthesis targeted by the E3 ubiquitin ligase MARCHF6 SC5D 是 E3 泛素连接酶 MARCHF6 针对的胆固醇生物合成过程中的第六种酶。
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-03-18 DOI: 10.1016/j.bbalip.2024.159482
Nicole M. Fenton, Lydia Qian, Nicola A. Scott , Eloise G. Paine, Laura J. Sharpe, Andrew J. Brown
{"title":"SC5D is the sixth enzyme in cholesterol biosynthesis targeted by the E3 ubiquitin ligase MARCHF6","authors":"Nicole M. Fenton,&nbsp;Lydia Qian,&nbsp;Nicola A. Scott ,&nbsp;Eloise G. Paine,&nbsp;Laura J. Sharpe,&nbsp;Andrew J. Brown","doi":"10.1016/j.bbalip.2024.159482","DOIUrl":"10.1016/j.bbalip.2024.159482","url":null,"abstract":"","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 4","pages":"Article 159482"},"PeriodicalIF":4.8,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198124000325/pdfft?md5=d1027cde48fc4ece20c71d7ac42444af&pid=1-s2.0-S1388198124000325-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140179340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “PNPLA-mediated lipid hydrolysis and transacylation – At the intersection of catabolism and anabolism” [Biochim. Biophys. Acta (BBA) – Mol. Cell Biol. Lipids volume 1869, issue 2, March 2024, 159,410] 更正:"PNPLA 介导的脂质水解和酰化--分解代谢和合成代谢的交叉点" [Biochim.Biophys.Acta (BBA) - Mol.细胞生物学。脂质》第 1869 卷第 2 期,2024 年 3 月,159,410 页]
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-03-15 DOI: 10.1016/j.bbalip.2024.159481
Mariana Colaço-Gaspar , Peter Hofer , Monika Oberer , Rudolf Zechner
{"title":"Corrigendum to “PNPLA-mediated lipid hydrolysis and transacylation – At the intersection of catabolism and anabolism” [Biochim. Biophys. Acta (BBA) – Mol. Cell Biol. Lipids volume 1869, issue 2, March 2024, 159,410]","authors":"Mariana Colaço-Gaspar ,&nbsp;Peter Hofer ,&nbsp;Monika Oberer ,&nbsp;Rudolf Zechner","doi":"10.1016/j.bbalip.2024.159481","DOIUrl":"https://doi.org/10.1016/j.bbalip.2024.159481","url":null,"abstract":"","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 4","pages":"Article 159481"},"PeriodicalIF":4.8,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198124000313/pdfft?md5=0c9da09098f7f5137c523131a8fc86d2&pid=1-s2.0-S1388198124000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140134450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular matrix hyaluronan modulates fat cell differentiation and primary cilia dynamics 细胞外基质透明质酸调节脂肪细胞分化和初级纤毛动力学
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-02-28 DOI: 10.1016/j.bbalip.2024.159470
Krzysztof Drygalski , Romane Higos , Fatiha Merabtene , Patrycja Mojsak , Kamil Grubczak , Michal Ciborowski , Hady Razak , Karine Clément , Isabelle Dugail
{"title":"Extracellular matrix hyaluronan modulates fat cell differentiation and primary cilia dynamics","authors":"Krzysztof Drygalski ,&nbsp;Romane Higos ,&nbsp;Fatiha Merabtene ,&nbsp;Patrycja Mojsak ,&nbsp;Kamil Grubczak ,&nbsp;Michal Ciborowski ,&nbsp;Hady Razak ,&nbsp;Karine Clément ,&nbsp;Isabelle Dugail","doi":"10.1016/j.bbalip.2024.159470","DOIUrl":"10.1016/j.bbalip.2024.159470","url":null,"abstract":"<div><p>Hyaluronan is an important extracellular matrix component, with poorly documented physiological role in the context of lipid-rich adipose tissue. We have investigated the global impact of hyaluronan removal from adipose tissue environment by <em>in vitro</em> exposure to exogenous hyaluronidase (or heat inactivated enzyme). Gene set expression analysis from RNA sequencing revealed downregulated adipogenesis as a main response to hyaluronan removal from human adipose tissue samples, which was confirmed by hyaluronidase-mediated inhibition of adipocyte differentiation in the 3T3L1 adipose cell line. Hyaluronidase exposure starting from the time of induction with the differentiation cocktail reduced lipid accumulation in mature adipocytes, limited the expression of terminal differentiation marker genes, and impaired the early induction of co-regulated <em>Cebpa</em> and <em>Pparg</em> mRNA. Reduction of <em>Cebpa</em> and <em>Pparg</em> expression by exogenous hyaluronidase was also observed in cultured primary preadipocytes from subcutaneous, visceral or brown adipose tissue of mice. Mechanistically, inhibition of adipogenesis by hyaluronan removal was not caused by changes in osmotic pressure or cell inflammatory status, could not be mimicked by exposure to threose, a metabolite generated by hyaluronan degradation, and was not linked to alteration in endogenous Wnt ligands expression. Rather, we observed that hyaluronan removal associated with disrupted primary cilia dynamics, with elongated cilium and higher proportions of preadipocytes that remained ciliated in hyaluronidase-treated conditions. Thus, our study points to a new link between ciliogenesis and hyaluronan impacting adipose tissue development.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 4","pages":"Article 159470"},"PeriodicalIF":4.8,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198124000209/pdfft?md5=af2dcee5ee75818f2cca33be87edf727&pid=1-s2.0-S1388198124000209-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-definition FT-IR reveals a synergistic effect on lipid accumulation in prostate cancer cells induced by a combination of X-rays and radiosensitizing drugs 高清傅立叶变换红外光谱揭示了 X 射线和放射增敏药物联合诱导前列腺癌细胞脂质积累的协同效应。
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-02-24 DOI: 10.1016/j.bbalip.2024.159468
Maciej Roman , Tomasz P. Wrobel , Agnieszka Panek , Wojciech M. Kwiatek
{"title":"High-definition FT-IR reveals a synergistic effect on lipid accumulation in prostate cancer cells induced by a combination of X-rays and radiosensitizing drugs","authors":"Maciej Roman ,&nbsp;Tomasz P. Wrobel ,&nbsp;Agnieszka Panek ,&nbsp;Wojciech M. Kwiatek","doi":"10.1016/j.bbalip.2024.159468","DOIUrl":"10.1016/j.bbalip.2024.159468","url":null,"abstract":"<div><p>Radiotherapy is one of the most commonly used cancer therapies with many benefits including low toxicity to healthy tissues. However, a major problem in radiotherapy is cancer radioresistance. To enhance the effect of this kind of therapy several approaches have been proposed such as the use of radiosensitizers. A combined treatment of radiotherapy and radiosensitizing drugs leads to a greater effect on cancer cells than anticipated from the addition of both responses (synergism). In this study, high-definition FT-IR imaging was applied to follow lipid accumulation in prostate cancer cells as a response to X-ray irradiation, radiosensitizing drugs, and a combined treatment of X-rays and the drugs. Lipid accumulation induced in the cells by an increasing X-ray dose and the presence of the drugs was analyzed using Principal Component Analysis and lipid staining. Finally, the synergistic effect of the combined therapy (X-rays and radiosensitizers) was confirmed by calculations of the integral intensity of the 2850 cm<sup>−1</sup> band.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 4","pages":"Article 159468"},"PeriodicalIF":4.8,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells 维生素 D 状态会改变麝鼠颗粒细胞中参与卵巢类固醇生成的基因
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-02-23 DOI: 10.1016/j.bbalip.2024.159469
Wenjing Lu, Yuan Chen, María Daniela Artigas Ramírez, Yuning Liu, Haolin Zhang, Zhengrong Yuan, Yingying Han, Qiang Weng
{"title":"Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells","authors":"Wenjing Lu,&nbsp;Yuan Chen,&nbsp;María Daniela Artigas Ramírez,&nbsp;Yuning Liu,&nbsp;Haolin Zhang,&nbsp;Zhengrong Yuan,&nbsp;Yingying Han,&nbsp;Qiang Weng","doi":"10.1016/j.bbalip.2024.159469","DOIUrl":"10.1016/j.bbalip.2024.159469","url":null,"abstract":"<div><p>This study aims to explore the relationship between altered vitamin D (VitD<sub>3</sub>) status and ovarian steroidogenesis in muskrats during the breeding and non-breeding seasons. During the breeding season, the ovaries of muskrats were observably enlarged and increased in weight, accompanied by elevated serum and ovarian VitD<sub>3</sub> status. Vitamin D receptor (VDR), VitD<sub>3</sub> metabolic molecules (CYP2R1, CYP27B1, and CYP24A1), and steroidogenic enzymes were immunolocalized in the ovarian cells of muskrats. The mRNA levels of <em>VDR</em>, <em>CYP2R1</em>, <em>CYP27B1</em>, and steroidogenic enzymes were considerably higher during the breeding season compared to the non-breeding season. RNA-seq analysis revealed a prominent enrichment of vitamin-related and ovarian steroidogenesis pathways. Furthermore, the addition of 1,25(OH)<sub>2</sub>D<sub>3</sub> to the muskrat granulosa cells <em>in vitro</em> increased VDR and steroidogenic enzymes mRNA levels and enhanced the 17β-estradiol level. Overall, these findings supported that VitD<sub>3</sub> promotes the secretion of steroid hormones, thereby affecting seasonal changes in ovarian function in the muskrats.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 4","pages":"Article 159469"},"PeriodicalIF":4.8,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139946901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specific labeling of newly synthesized lipopolysaccharide via metabolic incorporation of azido-galactose 通过叠氮半乳糖的代谢结合对新合成的脂多糖进行特异性标记
IF 4.8 2区 生物学
Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-02-19 DOI: 10.1016/j.bbalip.2024.159467
Yang Xu , Xiaoqi Wang , Esther A. Zaal , Celia R. Berkers , Joseph H. Lorent , Torben Heise , Ruud Cox , Roland J. Pieters , Eefjan Breukink
{"title":"Specific labeling of newly synthesized lipopolysaccharide via metabolic incorporation of azido-galactose","authors":"Yang Xu ,&nbsp;Xiaoqi Wang ,&nbsp;Esther A. Zaal ,&nbsp;Celia R. Berkers ,&nbsp;Joseph H. Lorent ,&nbsp;Torben Heise ,&nbsp;Ruud Cox ,&nbsp;Roland J. Pieters ,&nbsp;Eefjan Breukink","doi":"10.1016/j.bbalip.2024.159467","DOIUrl":"10.1016/j.bbalip.2024.159467","url":null,"abstract":"<div><p>Gram-negative bacteria possess an asymmetric outer membrane (OM) primarily composed of lipopolysaccharides (LPS) on the outer leaflet and phospholipids on the inner leaflet. The outer membrane functions as an effective permeability barrier to compounds such as antibiotics. Studying LPS biosynthesis is therefore helpful to explore novel strategies for new antibiotic development. Metabolic glycan labeling of the bacterial surface has emerged as a powerful method to investigate LPS biosynthesis. However, the previously reported methods of labeling LPS are based on radioactivity or difficult-to-produce analogs of bacterial sugars. In this study, we report on the incorporation of azido galactose into the LPS of the Gram-negative bacteria <em>Escherichia coli</em> and <em>Salmonella typhi</em> via metabolic labeling. As a common sugar analog, azido galactose successfully labeled both O-antigen and core of <em>Salmonella</em> LPS, but not <em>E. coli</em> LPS. This labeling of <em>Salmonella</em> LPS, as shown by SDS-PAGE analysis and fluorescence microscopy, differs from the previously reported labeling of either O-antigen or core of LPS. Our findings are useful for studying LPS biogenesis pathways in Gram-negative bacteria like <em>Salmonella</em>. In addition, our approach is helpful for screening for agents that target LPS biosynthesis as it allows for the detection of newly synthesized LPS that appears in the OM. Furthermore, this approach may also aid in isolating chemically modified LPS for vaccine development or immunotherapy.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 4","pages":"Article 159467"},"PeriodicalIF":4.8,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198124000179/pdfft?md5=4a82ed614aa7ec06eb7307a18b9cceeb&pid=1-s2.0-S1388198124000179-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139918605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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