Biochimica et biophysica acta. Molecular and cell biology of lipids最新文献

{"title":"Sex modulates the diet-induced changes to the plasma lipidome in a rat model of cardiorespiratory fitness","authors":"Johanna Y. Fleischman ,&nbsp;James L. Casey ,&nbsp;Jennifer L. Meijer ,&nbsp;Mary K. Treutelaar ,&nbsp;Thekkelnaycke M. Rajendiran ,&nbsp;Tanu Soni ,&nbsp;Charles R. Evans ,&nbsp;Charles F. Burant","doi":"10.1016/j.bbalip.2024.159451","DOIUrl":"10.1016/j.bbalip.2024.159451","url":null,"abstract":"<div><h3>Objective</h3><p>Individuals with higher intrinsic cardiorespiratory fitness (CRF) experience decreased rates of cardiometabolic disease and mortality, and high CRF is associated with increased utilization of fatty acids (FAs) for energy. Studies suggest a complex relationship between CRF, diet, and sex with health outcomes, but this interaction is understudied. We hypothesized that FA utilization differences by fitness and sex could be detected in the plasma metabolome when rats or humans were fed a high carbohydrate (HC) or high fat (HF) diet.</p></div><div><h3>Methods</h3><p>Male and female rats selectively bred for low (LCR) and high (HCR) CRF were fed a chow diet or a sucrose-free HF (45 % fat) or HC (10 % fat) diet. Plasma samples were collected at days 0, 3, and 14. Human plasma data was collected from male and female participants who were randomized into a HC or HF diet for 21 days. Samples were analyzed using liquid chromatography-mass spectrometry and regression statistics were used to quantify the effect of diet, CRF, and sex on the lipidome.</p></div><div><h3>Results</h3><p>In rats, the baseline lipidome is more significantly influenced by sex than by CRF, especially as elevated diglycerides, triglycerides, phosphatidylcholines, and lysophosphatidylcholines in males. A dynamic response to diet was observed 3 days after diet, but after 14 days of either diet, the lipidome was modulated by sex with a larger effect size than by diet. Data from the human study also suggests a sex-dependent response to diet with opposite directionality of affect compared to rats, highlighting species-dependent responses to dietary intervention.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 3","pages":"Article 159451"},"PeriodicalIF":4.8,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139373793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing and regulation of long-chain polyunsaturated fatty acids pool in marine mollusks: Characterization of UBXD8 from the razor clam Sinonovacula constricta","authors":"Zhaoshou Ran ,&nbsp;Haixuan Xie ,&nbsp;Xuxu Tian ,&nbsp;Fei Kong ,&nbsp;Kai Liao ,&nbsp;Xiaojun Yan ,&nbsp;Jilin Xu","doi":"10.1016/j.bbalip.2023.159448","DOIUrl":"10.1016/j.bbalip.2023.159448","url":null,"abstract":"<div><p>The razor clam <em>Sinonovacula constricta</em><span> is known for its richness in long-chain polyunsaturated fatty acids (LC-PUFA, C ≥ 20). Previously, we demonstrated that it possesses a complete LC-PUFA biosynthetic pathway. However, the mechanisms by which it senses the LC-PUFA pool to regulate their biosynthesis remain unclear. Here, we presented the LC-PUFA sensor UBXD8 as a critical molecule in this intriguing process. The </span><em>S. constricta</em> UBXD8 (<em>Sc</em><span>UBXD8) shared all characteristic features of its mammalian counterpart and exhibited high mRNA levels in digestive tissues, suggesting its functional role in this bivalve species. By purification of </span><em>Sc</em>UBXD8 protein <em>in vitro</em><span>, we discovered its ability to sense unsaturated fatty acids (UFA, C ≥ 14) but not saturated ones, as evidenced by polymerization detection. Furthermore, the intensity of </span><em>Sc</em>UBXD8 polymerization increased progressively with longer acyl chain lengths, greater unsaturation degrees, and higher UFA concentrations. Exceptionally, for those located at the same node in LC-PUFA biosynthetic pathway, <em>Sc</em>UBXD8 displayed a stronger sensitivity to n-6 UFA compared to n-3 UFA. These results suggested a critical role for <em>Sc</em><span>UBXD8 in balancing fatty acids composition and ratio of n-6/n-3 UFA in </span><em>S. constricta</em>. Moreover, the UAS domain was confirmed essential for <em>Sc</em>UBXD8 polymerization. Through knockdown of <em>Sc</em>Ubxd8 gene <em>in vivo</em>, there were significant shifts in expression patterns of genes related to LC-PUFA biosynthesis, concurrently influencing fatty acids compositions. These results suggested that <em>Sc</em>UBXD8 likely plays a regulatory role in LC-PUFA biosynthesis, possibly through the INSIG-SREBP pathway. Collectively, this study proposed that <em>S. constricta</em><span> might maintain LC-PUFA homeostasis through UBXD8 to regulate their biosynthesis.</span></p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159448"},"PeriodicalIF":4.8,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139092846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of novel diagnostic biomarkers for Sjögren-Larsson syndrome by untargeted lipidomics","authors":"Frédéric M. Vaz ,&nbsp;Pippa Staps ,&nbsp;Jan Bert van Klinken ,&nbsp;Henk van Lenthe ,&nbsp;Martin Vervaart ,&nbsp;Ronald J.A. Wanders ,&nbsp;Mia L. Pras-Raves ,&nbsp;Michel van Weeghel ,&nbsp;Gajja S. Salomons ,&nbsp;Sacha Ferdinandusse ,&nbsp;Ron A. Wevers ,&nbsp;Michèl A.A.P. Willemsen","doi":"10.1016/j.bbalip.2023.159447","DOIUrl":"10.1016/j.bbalip.2023.159447","url":null,"abstract":"<div><h3>Aim</h3><p>Sjögren-Larsson syndrome (SLS) is a rare neurometabolic disorder that mainly affects brain, eye and skin and is caused by deficiency of fatty aldehyde dehydrogenase. Our recent finding of a profoundly disturbed brain tissue lipidome in SLS prompted us to search for similar biomarkers in plasma as no functional test in blood is available for SLS.</p></div><div><h3>Methods and results</h3><p>We performed plasma lipidomics and used a newly developed bioinformatics tool to mine the untargeted part of the SLS plasma and brain lipidome to search for SLS biomarkers. Plasma lipidomics showed disturbed ether lipid metabolism in known lipid classes. Untargeted lipidomics of both plasma and brain (white and grey matter) uncovered two new endogenous lipid classes highly elevated in SLS. The first biomarker group were alkylphosphocholines/ethanolamines containing different lengths of alkyl-chains where some alkylphosphocholines were &gt; 600-fold elevated in SLS plasma. The second group of biomarkers were a set of 5 features of unknown structure. Fragmentation studies suggested that they contain ubiquinol and phosphocholine and one feature was also found as a glucuronide conjugate in plasma. The plasma features were highly distinctive for SLS with levels &gt;100–1000-fold the level in controls, if present at all. We speculate on the origin of the alkylphosphocholines/ethanolamines and the nature of the ubiquinol-containing metabolites.</p></div><div><h3>Conclusions</h3><p>The metabolites identified in this study represent novel endogenous lipid classes thus far unknown in humans. They represent the first plasma metabolite SLS-biomarkers and may also yield more insight into SLS pathophysiology.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159447"},"PeriodicalIF":4.8,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198123001713/pdfft?md5=28cc58c350aaf496cf5c9ec6eb1b54e0&pid=1-s2.0-S1388198123001713-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139092817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human ApoC3 overexpression aggravates hyperlipidemia but mitigates diet-induced coronary atherosclerotic disease in SR-BI and LDL receptor double knockout mice","authors":"Jiawei Liao ,&nbsp;Yuhui Wang ,&nbsp;Yao Wang ,&nbsp;Jinjin Zhang ,&nbsp;Feng Wu ,&nbsp;George Liu ,&nbsp;Wei Huang ,&nbsp;Ying Zhang","doi":"10.1016/j.bbalip.2023.159449","DOIUrl":"10.1016/j.bbalip.2023.159449","url":null,"abstract":"","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 3","pages":"Article 159449"},"PeriodicalIF":4.8,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139064526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in bile acid composition are correlated with reduced intestinal cholesterol uptake in intestine-specific WASH-deficient mice","authors":"Andries Heida ,&nbsp;Theo van Dijk ,&nbsp;Marieke Smit ,&nbsp;Martijn Koehorst ,&nbsp;Mirjam Koster ,&nbsp;Niels Kloosterhuis ,&nbsp;Rick Havinga ,&nbsp;Vincent W. Bloks ,&nbsp;Justina C. Wolters ,&nbsp;Alain de Bruin ,&nbsp;Jan Albert Kuivenhoven ,&nbsp;Jan Freark de Boer ,&nbsp;Folkert Kuipers ,&nbsp;Bart van de Sluis","doi":"10.1016/j.bbalip.2023.159445","DOIUrl":"10.1016/j.bbalip.2023.159445","url":null,"abstract":"<div><p>The Wiskott-Aldrich syndrome protein and SCAR homolog (WASH) complex is a pentameric protein complex localized at endosomes, where it facilitates the transport of numerous receptors from endosomes toward the plasma membrane. Recent studies have shown that the WASH complex plays an essential role in cholesterol and glucose homeostasis in humans and mice. To investigate the physiological importance of intestinal WASH, we ablated the WASH component WASHC1 specifically in murine enterocytes. Male and female intestine-specific WASHC1-deficient mice (<em>Washc1</em>^IKO) were challenged with either a standard chow diet or a high-cholesterol (1.25 %) diet (HCD). <em>Washc1</em>^IKO mice fed a standard diet did not present any apparent phenotype, but when fed an HCD, their hepatic cholesterol levels were ~ 50 % lower compared to those observed in control mice. The intestinal cholesterol absorption was almost 2-fold decreased in <em>Washc1</em>^IKO mice, which translated into increased fecal neutral sterol loss. The intestinal expression of cholesterogenic genes, such as <em>Hmgcs1</em>, <em>Hmgcr</em>, and <em>Ldlr</em>, was significantly higher in <em>Washc1</em>^IKO mice than in control mice and correlated with increased whole-body de novo cholesterol synthesis, likely to compensate for impaired intestinal cholesterol absorption. Unexpectedly, the ratio of biliary 12α−/non-12α-hydroxylated bile acids (BAs) was decreased in <em>Washc1</em>^IKO mice and reversing this reduced ratio by feeding the mice with the HCD supplemented with 0.5 % (<em>w</em>/w) sodium cholate normalized the improvement of hepatic cholesterol levels in <em>Washc1</em>^IKO mice. Our data indicate that the intestinal WASH complex plays an important role in intestinal cholesterol absorption, likely by modulating biliary BA composition.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159445"},"PeriodicalIF":4.8,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198123001695/pdfft?md5=85fb9a3ec7f964341f8d00bd741080ae&pid=1-s2.0-S1388198123001695-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138569898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatic oxylipin profiles in mouse models of Wilson disease: New insights into early hepatic manifestations","authors":"Tagreed A. Mazi ,&nbsp;Noreene M. Shibata ,&nbsp;Gaurav V. Sarode ,&nbsp;Valentina Medici","doi":"10.1016/j.bbalip.2023.159446","DOIUrl":"10.1016/j.bbalip.2023.159446","url":null,"abstract":"<div><p>Hepatic inflammation is commonly identified in Wilson disease (WD), a genetic disease of hepatic and brain copper accumulation. Copper accumulation is associated with increased oxidative stress and reactive oxygen species generation which may result in non-enzymatic oxidation of membrane-bound polyunsaturated fatty acids (PUFA). PUFA can be oxidized enzymatically via lipoxygenases (LOX), cyclooxygenases (COX), and cytochrome P450 monooxygenases (CYP). Products of PUFA oxidation are collectively known as oxylipins (OXL) and are bioactive lipids that modulate hepatic inflammation. We examined hepatic OXL profiles at early stages of WD in two mouse models, the toxic milk mouse from The Jackson Laboratory (tx-j) and the <em>Atp7b</em> knockout on a C57Bl/6 background (<em>Atp7b</em>^−/−B6). Targeted lipidomic analysis performed by ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry showed that in both tx-j and <em>Atp7b</em>^−/−B6 mice, hepatic OXL profiles were altered with higher thromboxane and prostaglandins levels. The levels of oxidative stress marker, 9-HETE were increased more markedly in tx-j mice. However, both genotypes showed upregulated transcript levels of many genes related to oxidative stress and inflammation. Both genotypes showed higher prostaglandins, thromboxin along with higher PUFA-derived alcohols, diols, and ketones with altered epoxides; the expression of <em>Alox5</em> was upregulated and many CYP-related genes were dysregulated. Pathway analyses show dysregulation in arachidonic acid and linoleic acid metabolism characterizes mice with WD. Our findings indicate alterations in hepatic PUFA metabolism in early-stage WD and suggest the upregulation of both, non-enzymatic ROS-dependent and enzymatic PUFA oxidation, which could have implications for hepatic manifestations in WD and represent potential targets for future therapies.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159446"},"PeriodicalIF":4.8,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198123001701/pdfft?md5=6c8ea604dc4bc9bb5a06e2e63f5c1d61&pid=1-s2.0-S1388198123001701-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138569897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ceramide regulation of autophagy: A biophysical approach","authors":"Yaiza R. Varela,&nbsp;Marina N. Iriondo,&nbsp;Félix M. Goñi,&nbsp;Alicia Alonso,&nbsp;L. Ruth Montes","doi":"10.1016/j.bbalip.2023.159444","DOIUrl":"10.1016/j.bbalip.2023.159444","url":null,"abstract":"<div><p>Specific membrane lipids play unique roles in (macro)autophagy. Those include phosphatidylethanolamine, to which LC3/GABARAP autophagy proteins become covalently bound in the process, or cardiolipin, an important effector in mitochondrial autophagy (or mitophagy). Ceramide (Cer), or N-acyl sphingosine, is one of the simplest sphingolipids, known as a stress signal in the apoptotic pathway. Moreover, Cer is increasingly being recognized as an autophagy activator, although its mechanism of action is unclear. In the present review, the proposed Cer roles in autophagy are summarized, together with some biophysical properties of Cer in membranes. Possible pathways for Cer activation of autophagy are discussed, including specific protein binding of the lipid, and Cer-dependent perturbation of bilayer properties. Cer generation of lateral inhomogeneities (domain formation) is given special attention. Recent biophysical results, including fluorescence and atomic force microscopy data, show Cer-promoted enhanced binding of LC3/GABARAP to lipid bilayers. These observations could be interpreted in terms of the putative formation of Cer-rich nanodomains.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159444"},"PeriodicalIF":4.8,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1388198123001683/pdfft?md5=7e64144e53370668f533480c94d9ca62&pid=1-s2.0-S1388198123001683-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138497726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis","authors":"Milena Krajewska ,&nbsp;Mariusz Możajew ,&nbsp;Sławomir Filipek ,&nbsp;Piotr Koprowski","doi":"10.1016/j.bbalip.2023.159443","DOIUrl":"10.1016/j.bbalip.2023.159443","url":null,"abstract":"<div><p><span>In this study, we utilized enzyme-catalyzed proximity labeling with the engineered promiscuous biotin<span> ligase<span> Turbo-ID to identify the proxisome of the ROMK2 channel. This channel resides in various cellular membrane compartments of the cell including the plasma membrane, endoplasmic reticulum and mitochondria. Within mitochondria, ROMK2 has been suggested as a pore-forming subunit of mitochondrial ATP-regulated potassium channel (mitoK</span></span></span>_ATP<span><span>). We found that ROMK2 proxisome in addition to previously known protein partners included two lipid<span><span> kinases: acylglycerol kinase (AGK) and diacylglycerol kinase ε (DGKE), which are localized in mitochondria and the endoplasmic reticulum, respectively. Through co-immunoprecipitation, we confirmed that these two kinases are present in complexes with ROMK2 channels. Additionally, we found that the products of AGK and DGKE, </span>lysophosphatidic acid (LPA) and </span></span>phosphatidic acid<span><span><span> (PA), stimulated the activity of ROMK2 channels in artificial lipid bilayers. Our </span>molecular docking studies revealed the presence of acidic lipid binding sites in the ROMK2 channel, similar to those previously identified in Kir2 channels. Based on these findings, we propose a model wherein localized </span>lipid synthesis, mediated by channel-bound lipid kinases, contributes to the regulation of ROMK2 activity within distinct intracellular compartments, such as mitochondria and the endoplasmic reticulum.</span></span></p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159443"},"PeriodicalIF":4.8,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138497727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMP deaminase: A crucial regulator in nitrogen stress and lipid metabolism in Mucor circinelloides","authors":"Shaoqi Li ,&nbsp;Junhuan Yang ,&nbsp;Hassan Mohamed ,&nbsp;Xiuwen Wang ,&nbsp;Wenyue Shi ,&nbsp;Futing Xue ,&nbsp;Sergio López-García ,&nbsp;Qing Liu ,&nbsp;Yuanda Song","doi":"10.1016/j.bbalip.2023.159434","DOIUrl":"10.1016/j.bbalip.2023.159434","url":null,"abstract":"<div><p><span>Lipid biosynthesis is a significant metabolic response to nitrogen starvation in oleaginous fungi. The oleaginous fungus </span><span><em>Mucor circinelloides</em></span><span><span> copes with nitrogen stress by degrading AMP through AMP deaminase (AMPD). However, the mechanism of AMPD in regulating lipogenesis remains largely unclear. To elucidate the mechanism of AMPD in </span>lipid synthesis in this </span><em>M. circinelloides</em>, we identified two genes (<em>ampd1</em> and <em>ampd2</em>) encoding AMPD and constructed an <em>ampd</em> double knockout mutant. The engineered <em>M. circinelloides</em><span><span><span> strain elevated cell growth and lipid accumulation, as well as the content of </span>oleic acid (OA) and gamma-linolenic acid (GLA). In addition to the expected increase in transcription levels of genes associated with lipid and </span>TAG<span><span><span> synthesis, we observed suppression of lipid degradation<span> and reduced amino acid biosynthesis. This suggested that the deletion of AMPD genes induces the redirection of carbon towards lipid synthesis pathways. Moreover, the pathways related to </span></span>nitrogen metabolism, including </span>nitrogen assimilation<span> and purine metabolism<span> (especially energy level), were also affected in order to maintain homeostasis. Further analysis discovered that the transcription factors (TFs) related to lipid accumulation were also regulated. This study provides new insights into lipid biosynthesis in </span></span></span></span><em>M. circinelloides</em>, indicating that the trigger for lipid accumulation is not entirely AMPD-dependent and suggest that there may be additional mechanisms involved in the initiation of lipogenesis.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159434"},"PeriodicalIF":4.8,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138486597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deficiency of Brummer lipase disturbs lipid mobilization and locomotion, and impairs reproduction due to defects in the eggshell ultrastructure in the insect vector Rhodnius prolixus","authors":"Daniela Saar Arêdes ,&nbsp;Thamara Rios ,&nbsp;Luiz Fernando Carvalho-Kelly ,&nbsp;Valdir Braz ,&nbsp;Luciana O. Araripe ,&nbsp;Rafaela V. Bruno ,&nbsp;José Roberto Meyer-Fernandes ,&nbsp;Isabela Ramos ,&nbsp;Katia C. Gondim","doi":"10.1016/j.bbalip.2023.159442","DOIUrl":"10.1016/j.bbalip.2023.159442","url":null,"abstract":"<div><p><span><em>Rhodnius prolixus</em></span><span> is a hematophagous insect, which feeds on large and infrequent blood meals, and is a vector of trypanosomatids<span><span><span> that cause Chagas disease. After feeding, lipids derived from blood meal are stored in the fat body as </span>triacylglycerol, which is recruited under conditions of energy demand by </span>lipolysis<span>, where the first step is catalyzed by the Brummer lipase (Bmm), whose orthologue in mammals is the adipose triglyceride lipase (ATGL). Here, we investigated the roles of Bmm in adult </span></span></span><em>Rhodnius prolixus</em> under starvation, and after feeding. Its gene (<em>RhoprBmm</em>) was expressed in all the analyzed insect organs, and its transcript levels in the fat body were not altered by nutritional status. RNAi-mediated knockdown of <em>RhoprBmm</em><span> caused triacylglycerol retention in the fat body during starvation, resulting in larger lipid droplets and lower ATP levels compared to control females. The silenced females showed decreased flight capacity and locomotor activity. When </span><em>RhoprBmm</em><span><span> knockdown occurred before the blood meal and the insects were fed, the females laid fewer eggs, which collapsed and showed low hatching rates. Their hemolymph had reduced diacylglycerol<span> content and vitellogenin concentration. The </span></span>chorion<span> (eggshell) of their eggs had no difference in hydrocarbon amounts or in dityrosine crosslinking levels compared to control eggs. However, it showed ultrastructural defects. These results demonstrated that Bmm activity is important not only to guarantee lipid mobilization to maintain energy homeostasis during starvation, but also for the production of viable eggs after a blood meal, by somehow contributing to the right formation of the egg chorion.</span></span></p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159442"},"PeriodicalIF":4.8,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138476678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}