Dominik Šťastný , Alena Balleková , Dana Tahotná , Lucia Pokorná , Roman Holič , Jana Humpolíčková , Peter Griač
{"title":"两种恶性疟原虫脂质转移蛋白(Sec14/CRAL-TRIO 家族)的特征。","authors":"Dominik Šťastný , Alena Balleková , Dana Tahotná , Lucia Pokorná , Roman Holič , Jana Humpolíčková , Peter Griač","doi":"10.1016/j.bbalip.2024.159572","DOIUrl":null,"url":null,"abstract":"<div><div>Invasion of human red blood cells by the malaria parasite <em>Plasmodium falciparum</em> is followed by dramatic modifications of erythrocytes properties, including <em>de novo</em> formation of new membrane systems. Lipid transfer proteins from both the parasite and the host cell are most likely an important part of those membrane remodeling processes. Using bioinformatics and <em>in silico</em> structural analysis, we have identified five <em>P. falciparum</em> potential lipid transfer proteins containing cellular retinaldehyde binding – triple functional domain (CRAL-TRIO). Two of these proteins, <span><span>C6KTD4</span><svg><path></path></svg></span>, encoded by the PF3D7_0629900 gene and <span><span>Q8II87</span><svg><path></path></svg></span>, encoded by the PF3D7_1127600 gene, were studied in more detail. <em>In vitro</em> lipid transfer assays using recombinant <span><span>C6KTD4</span><svg><path></path></svg></span> and <span><span>Q8II87</span><svg><path></path></svg></span> confirmed that these proteins are indeed <em>bona fide</em> lipid transfer proteins. <span><span>C6KTD4</span><svg><path></path></svg></span> transfers sterols, phosphatidylinositol 4,5 bisphosphate, and, to some degree, also phosphatidylcholine between two membrane compartments. <span><span>Q8II87</span><svg><path></path></svg></span> possesses phosphatidylserine transfer activity <em>in vitro</em>. In the yeast model, the expression of <em>P. falciparum</em> <span><span>Q8II87</span><svg><path></path></svg></span> protein partially complements the absence of Sec14p and its closest homologue, Sfh1p. <span><span>C6KTD4</span><svg><path></path></svg></span> protein can substitute for the collective essential function of oxysterol-binding related proteins. According to published whole genome studies in <em>P. falciparum</em>, absence of <span><span>C6KTD4</span><svg><path></path></svg></span> and <span><span>Q8II87</span><svg><path></path></svg></span> proteins has severe consequences for parasite viability. Therefore, CRAL-TRIO lipid transfer proteins of <em>P. falciparum</em> are potential targets of novel antimalarials, in search for which the yeast model expressing these proteins could be a valuable tool.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1870 1","pages":"Article 159572"},"PeriodicalIF":3.9000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of two Plasmodium falciparum lipid transfer proteins of the Sec14/CRAL-TRIO family\",\"authors\":\"Dominik Šťastný , Alena Balleková , Dana Tahotná , Lucia Pokorná , Roman Holič , Jana Humpolíčková , Peter Griač\",\"doi\":\"10.1016/j.bbalip.2024.159572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Invasion of human red blood cells by the malaria parasite <em>Plasmodium falciparum</em> is followed by dramatic modifications of erythrocytes properties, including <em>de novo</em> formation of new membrane systems. Lipid transfer proteins from both the parasite and the host cell are most likely an important part of those membrane remodeling processes. Using bioinformatics and <em>in silico</em> structural analysis, we have identified five <em>P. falciparum</em> potential lipid transfer proteins containing cellular retinaldehyde binding – triple functional domain (CRAL-TRIO). Two of these proteins, <span><span>C6KTD4</span><svg><path></path></svg></span>, encoded by the PF3D7_0629900 gene and <span><span>Q8II87</span><svg><path></path></svg></span>, encoded by the PF3D7_1127600 gene, were studied in more detail. <em>In vitro</em> lipid transfer assays using recombinant <span><span>C6KTD4</span><svg><path></path></svg></span> and <span><span>Q8II87</span><svg><path></path></svg></span> confirmed that these proteins are indeed <em>bona fide</em> lipid transfer proteins. <span><span>C6KTD4</span><svg><path></path></svg></span> transfers sterols, phosphatidylinositol 4,5 bisphosphate, and, to some degree, also phosphatidylcholine between two membrane compartments. <span><span>Q8II87</span><svg><path></path></svg></span> possesses phosphatidylserine transfer activity <em>in vitro</em>. In the yeast model, the expression of <em>P. falciparum</em> <span><span>Q8II87</span><svg><path></path></svg></span> protein partially complements the absence of Sec14p and its closest homologue, Sfh1p. <span><span>C6KTD4</span><svg><path></path></svg></span> protein can substitute for the collective essential function of oxysterol-binding related proteins. According to published whole genome studies in <em>P. falciparum</em>, absence of <span><span>C6KTD4</span><svg><path></path></svg></span> and <span><span>Q8II87</span><svg><path></path></svg></span> proteins has severe consequences for parasite viability. Therefore, CRAL-TRIO lipid transfer proteins of <em>P. falciparum</em> are potential targets of novel antimalarials, in search for which the yeast model expressing these proteins could be a valuable tool.</div></div>\",\"PeriodicalId\":8815,\"journal\":{\"name\":\"Biochimica et biophysica acta. 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Characterization of two Plasmodium falciparum lipid transfer proteins of the Sec14/CRAL-TRIO family
Invasion of human red blood cells by the malaria parasite Plasmodium falciparum is followed by dramatic modifications of erythrocytes properties, including de novo formation of new membrane systems. Lipid transfer proteins from both the parasite and the host cell are most likely an important part of those membrane remodeling processes. Using bioinformatics and in silico structural analysis, we have identified five P. falciparum potential lipid transfer proteins containing cellular retinaldehyde binding – triple functional domain (CRAL-TRIO). Two of these proteins, C6KTD4, encoded by the PF3D7_0629900 gene and Q8II87, encoded by the PF3D7_1127600 gene, were studied in more detail. In vitro lipid transfer assays using recombinant C6KTD4 and Q8II87 confirmed that these proteins are indeed bona fide lipid transfer proteins. C6KTD4 transfers sterols, phosphatidylinositol 4,5 bisphosphate, and, to some degree, also phosphatidylcholine between two membrane compartments. Q8II87 possesses phosphatidylserine transfer activity in vitro. In the yeast model, the expression of P. falciparumQ8II87 protein partially complements the absence of Sec14p and its closest homologue, Sfh1p. C6KTD4 protein can substitute for the collective essential function of oxysterol-binding related proteins. According to published whole genome studies in P. falciparum, absence of C6KTD4 and Q8II87 proteins has severe consequences for parasite viability. Therefore, CRAL-TRIO lipid transfer proteins of P. falciparum are potential targets of novel antimalarials, in search for which the yeast model expressing these proteins could be a valuable tool.
期刊介绍:
BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.