Biochimica et biophysica acta. Molecular and cell biology of lipids最新文献

{"title":"Gm15441 improves adipogenesis and insulin sensitivity by TXNIP regulation in white adipose tissue","authors":"Yinze Shi ,&nbsp;Liying Huang ,&nbsp;Xueyang Yang ,&nbsp;Jiaoyue Zhang ,&nbsp;Lulu Chen","doi":"10.1016/j.bbalip.2026.159718","DOIUrl":"10.1016/j.bbalip.2026.159718","url":null,"abstract":"<div><div><em>Gm15441</em>, a long non-coding RNA antisense to thioredoxin interacting protein (TXNIP) mRNA, exhibits undefined roles in adipogenesis and insulin resistance. This study aimed to explore its functions and mechanisms in white adipose tissue (WAT). <em>Gm15441</em> expression was assessed in 3T3-L1 cells and WAT of insulin-resistant mice. Stable <em>Gm15441</em> overexpression and knockdown 3T3-L1 cell models were established, followed by differentiation induction and analysis of lipid accumulation and differentiation markers. A subcutaneous adipose-specific <em>Gm15441</em> overexpression mouse model was fed high-fat diets (HFD) and evaluated for metabolic parameters, adipogenesis, and insulin signaling. Subcellular localization in vitro was determined via fluorescence in situ hybridization, while transcriptome sequencing, TXNIP expression analysis, and RNA-RNA pull-down assays were performed. Results showed that <em>Gm15441</em> expression increased during cell differentiation and decreased in insulin-resistant WAT. <em>Gm15441</em> overexpression promoted adipogenesis in vitro, while knockdown suppressed it. In HFD-fed mice, adipose-specific <em>Gm15441</em> overexpression enhanced adipogenesis, reduced blood glucose, and improved insulin sensitivity. Although PPARγ expression increased with cell differentiation, <em>Gm15441</em> probes did not pull down PPARγ mRNA. Conversely, TXNIP protein levels decreased in <em>Gm15441</em>-overexpressing cells without corresponding changes in mRNA levels, but <em>Gm15441</em> probes successfully pulled down TXNIP mRNA. These results suggested that <em>Gm15441</em> may promote adipogenesis and enhance insulin sensitivity by inhibiting TXNIP expression.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159718"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substituents of the polysaccharide region of LPS in Legionella pneumophila sg1 modulate interactions with host cells","authors":"Bożena Kowalczyk ,&nbsp;Agnieszka Szuster-Ciesielska ,&nbsp;Zbigniew Kaczyński ,&nbsp;Markus Petzold ,&nbsp;Christina E. Galuska ,&nbsp;Beate Fuchs ,&nbsp;Rafal Luchowski ,&nbsp;Wiesław I. Gruszecki ,&nbsp;Piotr Koper ,&nbsp;Adam Choma ,&nbsp;Jacek Tarasiuk ,&nbsp;Marta Palusińska-Szysz","doi":"10.1016/j.bbalip.2026.159720","DOIUrl":"10.1016/j.bbalip.2026.159720","url":null,"abstract":"<div><div><em>Legionella pneumophila</em> is a Gram-negative bacterium commonly found in natural freshwater reservoirs, where it exists as an intracellular parasite of cohabiting protozoa, primarily of the genus <em>Acanthamoeba</em>. Inhalation of aerosol contaminated with the bacterium leads to its proliferation within pulmonary macrophages, ultimately resulting in pneumonia known as Legionnaires' disease. The lipopolysaccharide (LPS) of <em>L. pneumophila</em> sg1 constitutes the most exposed component of the bacterial cell wall, playing a key role in every stage of its developmental cycle associated with host cells. The <em>orf8</em> gene encodes an <em>N</em>-methyltransferase responsible for the methylation of the 5-acetimidoylamino group in legionaminic acid of LPS of <em>L. pneumophila</em> sg1 strain 130b. Mutants impaired in synthesizing <em>N</em>-methyl groups of legionaminic acid produce neutral lipids, sphingolipids, and ceramides with an altered composition compared to the wild-type strain. <em>N</em>-methyl groups in legionaminic acid enhance bacterial adhesion to <em>Acanthamoeba castellanii</em> cells and THP-1-derived macrophages. Their occurrence, however, reduces the adhesion capacity of <em>L. pneumophila</em> sg1 strain 130b to epithelial cells of the A549 and BEAS-2B lines.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159720"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145986713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palmitoylated STX11 suppresses AMPK to drive lipogenesis and colorectal cancer","authors":"Bao Li ,&nbsp;Zhongkang Yan ,&nbsp;Wenyuan Dang ,&nbsp;Jianxiong Han ,&nbsp;Hongli Xue ,&nbsp;Feifei Wang ,&nbsp;Lili Wang ,&nbsp;Xueqing Yang ,&nbsp;Xingyuan Yang","doi":"10.1016/j.bbalip.2026.159730","DOIUrl":"10.1016/j.bbalip.2026.159730","url":null,"abstract":"<div><div>Syntaxin 11(STX11), a SNARE family protein, regulates vesicular trafficking and cytokinesis, yet its functional role in colorectal cancer (CRC) pathogenesis remains poorly understood. Here, we identify STX11 as a critical regulator that potentiates CRC progression in vivo and in vitro. Mechanistically, STX11 modulates the AMPK signaling pathway in a palmitoylation-dependent manner, attenuating ACC phosphorylation to enhance its enzymatic activity and stimulate de novo lipogenesis. Genetic ablation of STX11 significantly impedes tumorigenesis in an AOM/DSS-induced CRC mouse model. Our findings establish STX11 as a critical regulator of lipid metabolism in CRC progression and nominate it as a promising therapeutic target.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159730"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear phosphoinositides: An exploration into their regulation, roles and physico-chemical environment","authors":"Charlotte L. Collier ,&nbsp;Palita Udomjarumanee ,&nbsp;Lee Morgan ,&nbsp;Joaquin Cardenas Rodriguez ,&nbsp;Muhammad Bilal Qasim ,&nbsp;Bhavwanti Sheth ,&nbsp;Imogen Taylor ,&nbsp;Sonakshi Gehlot ,&nbsp;Stefano Leto ,&nbsp;Roberta Fiume ,&nbsp;Nullin Divecha","doi":"10.1016/j.bbalip.2026.159725","DOIUrl":"10.1016/j.bbalip.2026.159725","url":null,"abstract":"<div><div>Polyphosphoinositides are phospholipids consisting of a diacylglycerol backbone linked to an inositol headgroup that can be phosphorylated at three positions, generating seven distinct lipid species. Their levels are tightly regulated by coordinated kinase, phosphatase, and phospholipase activities, each responsive to diverse cellular cues. The functional diversity of phosphoinositides arises from their ability to bind specific protein domains, thereby influencing protein localisation, activity, and interaction networks. Although traditionally associated with membrane-bound compartments, phosphoinositides are also present within the nucleus, not only at the nuclear envelope but also within a variety of membrane-less nuclear structures. The identification of nuclear phosphoinositide-binding proteins has revealed that phosphoinositides contribute to the regulation of multiple nuclear processes, including transcription, RNA maturation and export, DNA damage responses, and broader nuclear stress signalling. In this review, we summarise the mechanisms by which nuclear phosphoinositides are generated, spatially organised, and interpreted by downstream effectors, and we highlight key outstanding questions that remain to be resolved.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159725"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidomic analysis of phospholipids and transcript expression of lipid metabolism genes in the liver and muscle of Atlantic salmon fed microbial oil and canola oil","authors":"Mohamed Emam ,&nbsp;JuDong Yeo ,&nbsp;Stefanie M. Colombo ,&nbsp;Nigel Guerra ,&nbsp;Khalil Eslamloo ,&nbsp;Albert Caballero-Solares ,&nbsp;Matthew L. Rise ,&nbsp;Christopher C. Parrish","doi":"10.1016/j.bbalip.2026.159723","DOIUrl":"10.1016/j.bbalip.2026.159723","url":null,"abstract":"<div><div>The increasing global demand for aquaculture products requires sustainable fish feed strategies. This study investigated how replacing fish oil with microbial oil (MO) and canola oil (CO) combinations affects lipid metabolism and gene expression in Atlantic salmon (<em>Salmo salar</em>), correlating these changes with phospholipid profiles. Four isonitrogenous, isoenergetic diets were tested: 20% fish oil (FO); 10% fish oil +10% CO (FO/CO); 15% CO + 5% MO (CO/MO); and 10% CO + 10% MO (MO-10). After 16 weeks, liver and skeletal muscle tissues were sampled for lipidomics and gene expression analysis. Multivariate analysis revealed distinct dietary group separations. The CO/MO diet induced the highest hepatic expression of <em>de novo</em> lipogenesis gene <em>fatty acid synthase b</em> while suppressing fatty acid oxidation marker <em>acyl-CoA oxidase 1</em>, indicating lipid storage promotion. Inflammatory marker <em>arachidonate 12-lipoxygenase</em> was associated with groups with reduced ω3 LC-PUFA (FO/CO, CO/MO). Muscle tissue showed subtler but diet-specific gene expression patterns, with <em>de novo</em> lipogenesis genes (<em>stearoyl-CoA desaturase b</em>, <em>ATP citrate lyase 2</em>) associated with decreased ω3 LC-PUFA (i.e., EPA and DHA) and correlating with monounsaturated fatty acids. The MO-10 group mirrored FO-fed fish, demonstrating successful fish oil replacement at 10% inclusion. Lipidomic pathway analysis revealed diet-induced phospholipid remodelling, for example, enhanced PE-to-PC conversion in the FO/CO and CO/MO groups, suggesting membrane-fluidity and inflammatory modulation. These results demonstrate tissue-specific metabolic adaptations to alternative lipid sources, with 10% CO + 10% MO effectively substituting for fish oil while maintaining metabolic profiles in the trial timeline. The findings advance our understanding of lipid metabolism regulation in salmon and support sustainable feed development for aquaculture and enhanced nutritional quality of aquaculture products.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159723"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting peroxisomal fatty acid β-oxidation lowers fasting glucose by suppressing gluconeogenesis","authors":"Wei Zhang ,&nbsp;Yicong Li ,&nbsp;Yida Zhang,&nbsp;Haoya Yao,&nbsp;Yaoqing Wang,&nbsp;Ping Li,&nbsp;Xiao Zhang,&nbsp;Jia Zeng","doi":"10.1016/j.bbalip.2026.159727","DOIUrl":"10.1016/j.bbalip.2026.159727","url":null,"abstract":"<div><div>Fatty acids play an important role in regulating gluconeogenesis through the metabolite acetyl-CoA, however, the underlying mechanisms on how fatty acid oxidation provides acetyl-CoA for the stimulation of pyruvate carboxylase and gluconeogenesis are not fully demonstrated. As a fatty acid β-oxidation system exists in peroxisomes and the acetyl-CoA derived from peroxisomal β-oxidation can be transported into mitochondria through the intermediate acetyl-carnitine, we hypothesize that this β-oxidation system might play a role in regulating pyruvate carboxylase and gluconeogenesis. The study demonstrates a mechanism by which fatty acids activate pyruvate carboxylase through the acetyl-CoA derived from peroxisomal β-oxidation. Induction of peroxisomal fatty acid β-oxidation results in excessive generation of acetyl-carnitine, which significantly elevates liver acetyl-CoA level and stimulates pyruvate carboxylase and gluconeogenesis in fasting mice. Specific inhibition of peroxisomal β-oxidation suppresses glucose production and lowers fasting glucose by reducing acetyl-CoA generation in the liver of diabetic mice. It is proposed that induction of peroxisomal β-oxidation serves as a pathogenic mechanism for fatty acids induced hyperactivation of pyruvate carboxylase and gluconeogenesis and targeting peroxisomal β-oxidation might be a potential pathway in treating diabetes through reducing acetyl-CoA generation and suppressing gluconeogenesis.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159727"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat therapy attenuates hepatic lipid accumulation via CSAD-driven metabolic reprogramming of fatty acid oxidation","authors":"Ang Li ,&nbsp;Yaru Dai ,&nbsp;Yijun Luo ,&nbsp;Ge Liang ,&nbsp;Luolan Gui ,&nbsp;Xinyi Hu ,&nbsp;Xin Li ,&nbsp;Dingkun Zhang ,&nbsp;Wen Zheng ,&nbsp;Lu Zhang ,&nbsp;Yuanping Gao ,&nbsp;Meng Gong","doi":"10.1016/j.bbalip.2026.159722","DOIUrl":"10.1016/j.bbalip.2026.159722","url":null,"abstract":"<div><h3>Objective</h3><div>Non-alcoholic fatty liver disease (NAFLD) represents a significant global health challenge, predominantly managed through non-pharmacological interventions. Heat therapy (HT) has been shown to effectively reduce hepatic lipid accumulation; however, the underlying mechanisms remain incompletely understood. This study aims to elucidate the mechanism by liver metabolomics.</div></div><div><h3>Methods</h3><div>This animal study utilized a high-fat diet to induce hepatic lipid accumulation in mice, combined with long-term HT intervention. Subsequently, metabolomic profiling and Western blot analysis were performed to assess hepatic metabolic alterations and elucidate the underlying mechanisms of HT action, followed by in vitro validation using L02 human hepatocytes.</div></div><div><h3>Results</h3><div>HT significantly decreased body weight, fat levels, and hepatic lipid accumulation while preventing excessive glycogen depletion in HFD-fed mice. Metabolomics analysis revealed that HT reduced diacylglycerol (DAG), triacylglycerol (TAG), free fatty acids (FFA), and saturated ceramides (CER); it also increased taurine levels, enhanced the utilization of vitamins B2, B5, and B6, and corrected amino acid imbalances. Furthermore, HT upregulated the expression of CSAD, ACADL, and ATP5A. In vitro studies demonstrated that heat treatment increased CSAD levels in L02 cells. Overexpression of CSAD elevated taurine levels and, under palmitic acid induction, reduced FFA levels while upregulating VDAC1 and ATP5A.</div></div><div><h3>Conclusions</h3><div>HT enhances mitochondrial function and promotes fatty acid oxidation by upregulating taurine synthesis via CSAD, thereby inhibiting hepatic lipid accumulation and correcting metabolic disorders, ultimately improving NAFLD.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159722"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palmitoylation of C4BPA in porcine epididymosomes mitigates complement C4-mediated damage to sperm","authors":"Heran Cao ,&nbsp;Long Li ,&nbsp;Shujuan Liu ,&nbsp;Hongmei Mo ,&nbsp;Yan Li ,&nbsp;Huihui Gao ,&nbsp;Tianhao Yang ,&nbsp;Mengqi Huang ,&nbsp;Ye Gong ,&nbsp;Zifang Wu ,&nbsp;Tianqi Jin ,&nbsp;Yang Wang ,&nbsp;Weibing Qin ,&nbsp;Wuzi Dong","doi":"10.1016/j.bbalip.2026.159729","DOIUrl":"10.1016/j.bbalip.2026.159729","url":null,"abstract":"<div><div>Previous studies have revealed the expression of anti-complement factors on the surface of sperm across various species to resist attacks from complement components in reproductive tracts. Here we show that the anti-complement factor The caput of the porcine epididymis specifically expresses C4BPA, which is then transported to the surface of sperm via epididymosomes. The presence of C4BPA in epididymosomes depends on its palmitoylation modification, specifically at the Cys13 and Cys23 residues. ZDHHC8 has been pinpointed as the palmitoyl transferase that carries out this modification. Palmitoylated C4BPA in epididymosomes significantly resists attacks by complement C4 on sperm, maintaining porcine sperm motility. Our findings reveal a critical role for palmitoylated C4BPA in mitigating C4-mediated damage to sperm, highlighting its physiological relevance in preserving sperm motility and viability.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 3","pages":"Article 159729"},"PeriodicalIF":3.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146096792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of macroautophagy and microautophagic lipophagy by phosphatidylserine synthase Cho1 and external ethanolamine","authors":"Nanaru Mineoka ,&nbsp;Rikako Konishi ,&nbsp;Yuri Nakashima ,&nbsp;Moe Muramoto ,&nbsp;Kayoko Fukuda ,&nbsp;Sayuri Kuriyama ,&nbsp;Tatsunori Masatani ,&nbsp;Akikazu Fujita","doi":"10.1016/j.bbalip.2025.159702","DOIUrl":"10.1016/j.bbalip.2025.159702","url":null,"abstract":"<div><div>Phospholipids play crucial roles in autophagy; however, the underlying mechanisms remain elusive. We previously found that the phosphatidylserine (PtdSer) transporter Osh5 is critical for autophagosome formation. Therefore, in this study, we aimed to investigate the impact of the knockout of <em>cho1</em>, which encodes PtdSer synthase, on autophagy. Green fluorescent protein-autophagy-related gene 8 (GFP-Atg8) processing assay revealed a significant defect in the macroautophagic activity of the <em>cho1</em>∆ mutant, regardless of the presence or absence of ethanolamine (Etn). Notably, autophagosomes were absent in the cytosol, and macroautophagic bodies were not observed in the vacuoles of the starved <em>cho1</em>∆ mutant, underscoring the essential role of PtdSer synthesized using Cho1 in autophagosome biogenesis. In contrast, numerous microautophagic vesicles containing lipid droplets were observed in the vacuoles of <em>cho1</em>∆ mutants starved in the presence of Etn, suggesting the crucial role of phosphatidylethanolamine (PtdEtn) synthesized via the Kennedy pathway in microautophagic lipophagy when PtdSer synthesis using Cho1 is disrupted. Given recent evidence pointing to the involvement of the ubiquitination system in various autophagy-related processes, we also examined the role of ubiquitin-conjugating enzyme E2 gene <em>ubc4</em>. In addition, deletion of <em>ubc4</em> gene led to a pronounced reduction in microautophagic lipophagy in starved <em>cho1</em>∆ cells, but not in wild-type cells. Together, these observations highlight an essential role for Ubc4-mediated ubiquitination in driving vacuolar microautophagic lipophagy specifically under Cho1-deficient conditions.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 2","pages":"Article 159702"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145426360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphoinositides at 50: From minor lipids to master organizers of polarity","authors":"Ama Gassama-Diagne","doi":"10.1016/j.bbalip.2025.159707","DOIUrl":"10.1016/j.bbalip.2025.159707","url":null,"abstract":"<div><div>Phosphoinositides (PIs) have been at the center of cell signaling and membrane biology since Michell's proposal of the PI-cycle in 1975. Over the past fifty years, these quantitatively minor lipids have emerged as versatile molecular organizers, defining membrane identity, generating spatial cues, coordinating cytoskeletal dynamics and thus playing pivotal role in polarity. Studies in yeast, amoebae, and neutrophils demonstrated how localized PI pools regulate polarity and chemotaxis, providing a conceptual advance to tissue-level organization. In epithelia, PIs now stand as central determinants of apico-basal polarity and lumen morphogenesis through their cooperation with Rho GTPases, polarity complexes, and septins. This review highlights how these lipids shape our understanding of membrane dynamics and epithelial tissue organization. To conclude, this review underscores how alterations in the activities of these polarity regulators lead to abnormal epithelial morphogenesis and severe diseases such as cancer.</div></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1871 2","pages":"Article 159707"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145548064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}