Biochimica et biophysica acta. Molecular and cell biology of lipids最新文献

Specific activity of mouse liver desaturases and elongases: Time course effects using n-3 and n-6 PUFA substrates and inhibitory responses of delta-6 desaturase.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2025-01-10 DOI: 10.1016/j.bbalip.2025.159594

Rodrigo Valenzuela, Adam H Metherel, Giulia Cisbani, Mackenzie E Smith, Raphaël Chouinard-Watkins, Brinley J Klievik, Camila Farias, Luis A Videla, Richard P Bazinet

{"title":"Specific activity of mouse liver desaturases and elongases: Time course effects using n-3 and n-6 PUFA substrates and inhibitory responses of delta-6 desaturase.","authors":"Rodrigo Valenzuela, Adam H Metherel, Giulia Cisbani, Mackenzie E Smith, Raphaël Chouinard-Watkins, Brinley J Klievik, Camila Farias, Luis A Videla, Richard P Bazinet","doi":"10.1016/j.bbalip.2025.159594","DOIUrl":"https://doi.org/10.1016/j.bbalip.2025.159594","url":null,"abstract":"The synthesis of n-3 and n-6 polyunsaturated acids (PUFAs) is associated with physiological functions in mammals, being catalyzed by Δ-5D and Δ-6D desaturases and elongases Elovl-2 and Elovl-5. In this context, we aimed to study the chief kinetic features of PUFA liver anabolism, looking upon (i) the time-dependency for the specific activity of Δ-6D, Δ-5D, Elovl2, Elovl2/5 and Elovl5, using n-3 and n-6 precursors between 0 and 240 min ex vivo in mouse liver.; and (ii) the specific activity-substrate (α-linolenic acid; ALA) concentration responses of Δ-6D in the absence and presence of linoleic acid (LA), arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), an enzyme regarded as the rate-limiting step in PUFA anabolism. Mouse liver was obtained from eight-week-old Balb/c mice fed a chow diet (expressed as % of total calories: 18 % fat, 24 % protein, and 58 % carbohydrate, with a caloric value of 3.1 kcal/g) for eight weeks, and used for preparation of the microsomal fraction. Enzymatic activities assayed under the addition of specific PUFA precursors or LA, ARA, EPA and DHA, identifying the respective PUFA products as fatty acid methyl esters by gas chromatographic analysis. Data described corroborate that (i) PUFA metabolism mainly occurs in the liver, with the participating enzymes preferring n-3 than n-6 substrates; and show that (ii) the rate-limiting step of PUFA metabolism relies on the second reaction of Δ-6D (24:5n-3 transformed to 24:6n-3); and (iii) LA, ARA, EPA and DHA act as non-competitive inhibitors with respect to ALA in the reaction catalyzed by Δ-6D. These results are relevant for future studies concerning the metabolic and nutritional implications of changes in desaturation and elongation of PUFAs.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1870 2","pages":"159594"},"PeriodicalIF":3.9,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the potential contribution of DHHC2 in cancer biology via untargeted metabolomics.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2025-01-08 DOI: 10.1016/j.bbalip.2025.159593

Suchi Chaturvedi, S Sibi Karthik, Sushabhan Sadhukhan, Avinash Sonawane

{"title":"Unraveling the potential contribution of DHHC2 in cancer biology via untargeted metabolomics.","authors":"Suchi Chaturvedi, S Sibi Karthik, Sushabhan Sadhukhan, Avinash Sonawane","doi":"10.1016/j.bbalip.2025.159593","DOIUrl":"10.1016/j.bbalip.2025.159593","url":null,"abstract":"DHHC-mediated protein-S-palmitoylation is recognized as a distinct and reversible lipid modification, playing a pivotal role in the progression and prevention of multiple diseases, including cancer and neurodegenerative disorders. Over the past decade, growing evidence indicated the crucial role of DHHC2 in preventing tumorigenesis by palmitoylation of various protein substrates. However, a comprehensive understanding of the specific impact of DHHC2 on cancer cell metabolic regulation remains unclear. To investigate the metabolic changes by DHHC2, we conducted untargeted metabolomic profiling on the HEK-293T cell line with DHHC2-Knockdown (DHHC2-KD), DHHC2-Overexpression (DHHC2-OE) and empty vector control (Ctrl) conditions via LC-MS/MS-based analysis. Our dataset revealed the identification of a total of 73 metabolites encompassing all the conditions, with only 22 showing significant differences in univariate analysis. Furthermore, we performed pathway analysis with metabolites having VIP ≥ 0.7, P value ≤ 0.05, and fold change (FC) > 2 in DHHC2-OE (upregulated) and FC < 0.5 in DHHC2-OE or FC > 2 in DHHC2-KD condition (downregulated). We unveiled significant expression of the pyrimidine metabolism, urea cycle, and aspartate metabolism due to the abundance of onco-metabolites such as glutamine, uridine, and glutamic acid in the DHHC2-KD condition. However, DHHC2 overexpression resulted in a higher expression of metabolites previously reported to be associated with anti-cancer activity, such as betaine and 5'-methylthioadenosine (5'-MTA). Overall, this study sheds light on the changes mediated by DHHC2 in a cancer cell metabolome and suggests avenues for further investigation into other DHHC isoforms and their metabolic aspects.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159593"},"PeriodicalIF":3.9,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay of transcriptional regulator SREBP1 with AMPK promotes lipid biosynthesis in Mucor circinelloides WJ11.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-27 DOI: 10.1016/j.bbalip.2024.159592

Tahira Naz, Xiang Yu Zhao, Shaoqi Li, Tariq Saeed, Samee Ullah, Yusuf Nazir, Qing Liu, Hassan Mohamed, Yuanda Song

{"title":"The interplay of transcriptional regulator SREBP1 with AMPK promotes lipid biosynthesis in Mucor circinelloides WJ11.","authors":"Tahira Naz, Xiang Yu Zhao, Shaoqi Li, Tariq Saeed, Samee Ullah, Yusuf Nazir, Qing Liu, Hassan Mohamed, Yuanda Song","doi":"10.1016/j.bbalip.2024.159592","DOIUrl":"10.1016/j.bbalip.2024.159592","url":null,"abstract":"SREBP1 is a transcription factor that influences lipogenesis by regulating key genes associated with lipid biosynthesis, while AMPK, modulates lipid metabolism by regulating acetyl-CoA carboxylase. The exact role of these metabolic regulators in oleaginous microbes remains unclear. This study identified and manipulated the genes encoding SREBP1 (sre1) and α1 subunit of AMPK (ampk-α1) in Mucor circinelloides WJ11. Individual overexpression of sre1 yielded 32.5 % lipids and 21 g/L biomass, while ampk-α1 deletion combined with sre1 overexpression yielded 42.5 % lipids and 25 g/L biomass in mutant strains. This increase correlated with upregulated expression of key lipogenic genes and enzyme activity, enhancing lipid production and biomass. These surges were correlated with the increased mRNA levels of key genes (acl, acc1, acc2, cme1, fas1, g6pdh1, g6pdh2 and 6pgdh2). Enzyme activity analysis further showed that upregulation of ACL, ACC, ME, FAS, G6PDH and 6PGDH might provide more precursors and NADPH for lipid biosynthesis in sre1 overexpressing strains. Conversely, the activities of these genes and enzymes were markedly downregulated in sre1 deleted mutants consistent with lower lipid production and biomass than the control. These findings open new avenues for research by exploring the coordinated role of sre1 and ampk-α1 in lipid metabolism in M. circinelloides.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159592"},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of SUR2 alters the composition of ceramides and shortens chronological lifespan of Saccharomyces cerevisiae.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-22 DOI: 10.1016/j.bbalip.2024.159591

Zhitao Deng, Qianqian Wang, Rongbin Ding, Weiwei Nie, Xiaoyan Chen, Yu Chen, Yanlu Wang, Jingjing Duan, Zhenying Hu

{"title":"Loss of SUR2 alters the composition of ceramides and shortens chronological lifespan of Saccharomyces cerevisiae.","authors":"Zhitao Deng, Qianqian Wang, Rongbin Ding, Weiwei Nie, Xiaoyan Chen, Yu Chen, Yanlu Wang, Jingjing Duan, Zhenying Hu","doi":"10.1016/j.bbalip.2024.159591","DOIUrl":"10.1016/j.bbalip.2024.159591","url":null,"abstract":"Sphingolipids are crucial components of cell membranes and serve as important signaling molecules. Ceramide, as the central hub of sphingolipid metabolism, plays a significant role in various biological processes, including the cell cycle, apoptosis, and cellular aging. Alterations in sphingolipid metabolism are implicated in cellular aging, however, the specific sphingolipid components and intrinsic mechanisms that mediate this process remain largely uncharacterized. In this study, we established a targeted sphingolipidomics approach and employed LC-MS/MS to quantitatively analyze changes in ceramide levels during chronological aging and in sur2Δ strains, aiming to elucidate the role of ceramides in regulating chronological lifespan. Our study revealed that in Saccharomyces cerevisiae, the C4 hydroxylase Sur2 and its product, phytoceramide, increase during chronological aging. While the loss of SUR2 function leads to a near-complete loss of phytoceramides and an accumulation of dihydroceramides, resulting in a significant reduction of total ceramide content to about half of that in wild-type cells. This ceramide profile alteration impairs both mitochondrial morphology and function, ultimately shortening the chronological lifespan. The knockout of SIT4 restores mitochondrial morphology and function, and rescues the chronological lifespan of SUR2-deficient yeast. Our findings highlight the critical role of dihydroceramide and phytoceramide in chronological aging in yeast and suggest that an imbalance between these two metabolites may trigger downstream ceramide signaling pathways. These insights could help elucidate potential mechanisms through which ceramide imbalance contributes to disease development in higher organisms.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159591"},"PeriodicalIF":3.9,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hippo pathway activation causes multiple lipid derangements in a murine model of cardiomyopathy.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-19 DOI: 10.1016/j.bbalip.2024.159590

Wei Wu, Kevin Huynh, Jin-Chan Du, Gang She, Thy Duong, Mark Ziemann, Wei-Bo Zhao, Xiu-Ling Deng, Peter J Meikle, Xiao-Jun Du

{"title":"Hippo pathway activation causes multiple lipid derangements in a murine model of cardiomyopathy.","authors":"Wei Wu, Kevin Huynh, Jin-Chan Du, Gang She, Thy Duong, Mark Ziemann, Wei-Bo Zhao, Xiu-Ling Deng, Peter J Meikle, Xiao-Jun Du","doi":"10.1016/j.bbalip.2024.159590","DOIUrl":"10.1016/j.bbalip.2024.159590","url":null,"abstract":"Metabolic reprogramming occurs in cardiomyopathy and heart failure contributing to progression of the disease. Activation of cardiac Hippo pathway signaling has been implicated in mediating mitochondrial dysfunction and metabolic reprogramming in cardiomyopathy, albeit influence of Hippo pathway on lipid profile is unclear. Using a dual-omics approach, we determined alterations of cardiac lipids in a mouse model of cardiomyopathy due to enhanced Hippo signaling and explored molecular mechanisms. Lipidomic profiling discovered multiple alterations in lipid classes, notably reduction of triacylglycerol, diacylglycerol, phospholipids and ether lipids, and elevation of sphingolipids and lysophosphatidylcholine. Mechanistically, we found downregulated expression of PPARα and PGC-1α at mRNA and protein levels, and downregulated expression of PPARα-target genes, indicating attenuated transcriptional activity of PPARα/PGC-1α. Lipidomics-guided transcriptomic analysis revealed dysregulated expression of gene sets that were responsible for enhanced biosynthesis of ceramides, suppression of TG biosynthesis, storage, hydrolysis and mitochondrial fatty acid oxidation, and reduction of peroxisome-localized biosynthesis of ether lipids. Collectively, Hippo pathway activation with attenuated PPARα/PGC-1α signaling is the underlying mechanism for alterations in cardiac lipids in cardiomyopathy and failing heart.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159590"},"PeriodicalIF":3.9,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression, purification and characterization of a dual function α-dioxygenase/peroxidase from Mycolicibacterium smegmatis. 烟曲霉中一种具有双重功能的 α-二氧化酶/过氧化物酶的表达、纯化和特征描述。

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-18 DOI: 10.1016/j.bbalip.2024.159587

Teresa Rotolo, Anna Kaye, Lauren Fahrenkrog, Kate Flynn, Elisabeth C Ford, Barry S Selinsky

{"title":"Expression, purification and characterization of a dual function α-dioxygenase/peroxidase from Mycolicibacterium smegmatis.","authors":"Teresa Rotolo, Anna Kaye, Lauren Fahrenkrog, Kate Flynn, Elisabeth C Ford, Barry S Selinsky","doi":"10.1016/j.bbalip.2024.159587","DOIUrl":"10.1016/j.bbalip.2024.159587","url":null,"abstract":"An open reading frame from the actinobacterium Mycolicibacterium smegmatis annotated as a Prostaglandin H Synthase (PGHS) was expressed with an N-terminal (his)6 tag and purified to homogeneity. The enzyme has a monomeric molecular weight of 68.3 kD and exists as a dimer in the presence of nonionic detergent. The enzyme uses saturated and unsaturated fatty acids as substrates and catalyzes two reactions: the addition of molecular oxygen alpha to the carboxylate group to form the 2-hydroperoxy fatty acid, followed by reduction to the 2-hydroxy fatty acid. The initial reduction reaction does not require a source of electrons, but electrons must be provided from an appropriate donor such as epinephrine for the reduction reaction to go to completion. Minor reaction products one carbon atom shorter than the original fatty acid substrate are also observed; These most likely arise from the spontaneous decarboxylation of the 2-hydroperoxy fatty acid product to form an aldehyde. This dual function dioxygenase/peroxidase is unusual among the lipid dioxygenases and may represent a bacterial precursor to mammalian PGHS.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159587"},"PeriodicalIF":3.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of glucose selenol on hepatic lipid metabolism disorder induced by heavy metal cadmium in male rats. 葡萄糖硒醇对重金属镉诱导的雄性大鼠肝脂代谢紊乱的影响

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-13 DOI: 10.1016/j.bbalip.2024.159589

Xinyi Yang, Jinzhou Huang, Juan Wang, Huimin Sun, JinJin Li, Shunfeng Li, Yun-E Tang, Zhi Wang, Qisheng Song

{"title":"Effect of glucose selenol on hepatic lipid metabolism disorder induced by heavy metal cadmium in male rats.","authors":"Xinyi Yang, Jinzhou Huang, Juan Wang, Huimin Sun, JinJin Li, Shunfeng Li, Yun-E Tang, Zhi Wang, Qisheng Song","doi":"10.1016/j.bbalip.2024.159589","DOIUrl":"10.1016/j.bbalip.2024.159589","url":null,"abstract":"This study used 24 male rats to determine the protective effects of a new selenium molecule (glucose selenol) on cadmium (Cd) induced hepatic toxicity. The rats were randomly divided into four groups: control group, Cd group, Cd + 0.15 Se group, and Cd + 0.4 Se group. The results showed that glucose selenol supplementation alleviated the adverse impact of Cd on lipid metabolism, including decreased serum triacylglycerol and cholesterol levels. Transcriptome analysis revealed that, compared to the control group, Cd changed the expression of 1379 genes - discernibly affecting lipid metabolism pathways. Proteomic analysis primarily indicated alterations in lipid metabolism-related pathways. In conclusion, glucose selenol restored lipid metabolism disorders induced by Cd, thus rescuing hepatic damage. This integrated analysis identified the influence of glucose selenol on Cd-induced hepatic toxicity and provided its potential application prospects in alleviating the impact of heavy metal pollution, such as Cd, on human health.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159589"},"PeriodicalIF":3.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of N-glycosylation on secretion, degradation and lipoprotein distribution of human serum amyloid A4. N-糖基化对人血清淀粉样蛋白 A4 的分泌、降解和脂蛋白分布的影响。

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-11 DOI: 10.1016/j.bbalip.2024.159588

Toru Takarada, Rikako Fujinaka, Masaki Shimada, Masakazu Fukuda, Toshiyuki Yamada, Masafumi Tanaka

{"title":"Effect of N-glycosylation on secretion, degradation and lipoprotein distribution of human serum amyloid A4.","authors":"Toru Takarada, Rikako Fujinaka, Masaki Shimada, Masakazu Fukuda, Toshiyuki Yamada, Masafumi Tanaka","doi":"10.1016/j.bbalip.2024.159588","DOIUrl":"10.1016/j.bbalip.2024.159588","url":null,"abstract":"Serum amyloid A (SAA) is a family of apolipoproteins predominantly synthesized and secreted by the liver. Human SAA4 is constitutively expressed and contains an N-glycosylation site that is not present in other SAA subtypes. SAA4 proteins are not fully glycosylated, resulting in the presence of both glycosylated and non-glycosylated forms in human plasma. The efficiency of N-glycosylation in SAA4 is known to be influenced by some reasons such as genetic polymorphism and metabolic disorders. However, the specific role of N-glycosylation in SAA4 remains largely unexplored. This study aimed to investigate how N-glycosylation affects the secretion, degradation, and lipoprotein distribution of SAA4. Initially, we designed and constructed an SAA4 plasmid vector to compare with the expression pattern of endogenous SAA4. The exogenous SAA4 was partially N-glycosylated, analogous to endogenous SAA4 in human hepatocellular carcinoma cells. Subsequently, we created a non-glycosylated mutant by replacing asparagine 76 with glutamine. Immunoblotting assays showed that the disruption of N-glycans did not affect the secretion and degradation of SAA4. Furthermore, we analyzed the lipoprotein profiles of SAA4 in the conditioned medium derived from transfected cells. The results revealed that non-glycosylated mutant SAA4 exhibited a distinct lipoprotein distribution compared to wild-type SAA4. Our findings suggest that N-glycosylation may be a key regulator of the distribution of SAA4 in lipoproteins, shedding light on the previously unknown physiological activities of human SAA4.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159588"},"PeriodicalIF":3.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Origin and evolution of yeast carotenoid pathways.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-10 DOI: 10.1016/j.bbalip.2024.159586

Gerhard Sandmann

{"title":"Origin and evolution of yeast carotenoid pathways.","authors":"Gerhard Sandmann","doi":"10.1016/j.bbalip.2024.159586","DOIUrl":"10.1016/j.bbalip.2024.159586","url":null,"abstract":"Carotenoid pathways exist in nature in all domains. Comparison of the genes involved and their distribution allowed the elucidation of the origin and evolution of carotenoid biosynthesis from an early common ancestor of prokaryotes to Bacteria and Archaea. From the latter domain, carotenogenic genes are inherited by fungi as the only phylum of Eukarya. Carotenoid biosynthesis in the algal-plant lineage emerged independently by endosymbiotic gene transfer from an engulfed carotenogenic cyanobacterium. The early set of carotenogenic genes included crtB of phytoene synthase, the desaturase gene crtI, and the lycopene cyclase gene crtYcd for the synthesis of β-carotene. This carotenoid is further metabolised either to zeaxanthin and retinal due to the presence of crtZ and ccd or elongated to a C50 carotenoids by the crtEb gene product. The diversified pathways, especially in bacteria and fungi, result from gene modifications altering the substrate and product specificities of the corresponding enzymes or from the acquisition of novel genes. This was highlighted in more detail for the carotenoid pathways in the red yeasts of Basidiomycota leading to torularhodin, 2'-plectaniaxanthin, and astaxanthin.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159586"},"PeriodicalIF":3.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptomic analysis and luteolin treatment reveal three adipogenic genes, including Aspn, Htra1 and Efemp1.

IF 3.9 2区生物学

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-12-09 DOI: 10.1016/j.bbalip.2024.159585

Tao Tao, Yanting Xu, Cheng-Hui Zhang, Xian Zhang, Juan Chen, Jian Liu

{"title":"Single-cell transcriptomic analysis and luteolin treatment reveal three adipogenic genes, including Aspn, Htra1 and Efemp1.","authors":"Tao Tao, Yanting Xu, Cheng-Hui Zhang, Xian Zhang, Juan Chen, Jian Liu","doi":"10.1016/j.bbalip.2024.159585","DOIUrl":"10.1016/j.bbalip.2024.159585","url":null,"abstract":"A comparative transcriptomic analysis in adipose stem and progenitor cells (ASPCs) between obese and lean mice might facilitate the identification of novel adipogenic genes. Here, we compare transcriptomic differences in the ASPCs of subcutaneous adipose tissue (SAT) between the mice fed on a high-fat-diet (HFD) and the chow diet (CD)-fed mice by analyzing three independent single-cell RNA sequencing datasets. Six differential genes, including three up-regulated genes Aspn, Rrbp1, Fbln2 and three down-regulated genes Htra1, Plpp3, Efemp1, are identified and confirmed in HFD-fed mice. Further, the expression of these genes is found to be significantly diminished in the differentiated 3T3-L1 cells. Treatment with luteolin, a dietary flavonoid known to inhibit 3T3-L1 adipogenesis, reverses the decreased expression of Aspn, Htra1 and Efemp1. Furthermore, knockdown of Aspn, Htra1 and Efemp1 significantly facilitates 3T3-L1 adipogenesis. Together, these genes not only are differential in ASPCs between obese and lean mice, but also are the adipogenic inhibitory genes that can be up-regulated by luteolin treatment.","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":" ","pages":"159585"},"PeriodicalIF":3.9,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0