载脂蛋白ob：脂蛋白生物学中细胞内蛋白检测的困境。

IF 3.3 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2025-09-13 DOI:10.1016/j.bbalip.2025.159690

Ankia Visser , Floris A. Feiner , Willemien Heerema-van Zwol , Marieke Smit , Nicolette C.A. Huijkman , Niels J. Kloosterhuis , Henry N. Ginsberg , Bart van de Sluis , Jan Albert Kuivenhoven

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引用次数: 0

摘要

•研究极低密度脂蛋白（VLDL）的细胞内生物学仍然是一个挑战。因此，我们产生了apoB100- flag小鼠，其血浆中显示正常的脂质水平和flag标记的apoB100浓度。然而，我们无法使用免疫荧光检测原代肝细胞中的载脂蛋白ob或FLAG。•单独阻断蛋白酶体降解或联合抑制分泌途径仅能略微提高载脂蛋白ob的检测。•我们的研究表明，分离肝细胞中载脂蛋白100的生理浓度过低且不均匀，无法进行载脂蛋白b亚细胞定位的研究。

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ApoB: The intracellular protein detection dilemma in lipoprotein biology

•
Investigating the intracellular biology of very low-density lipoproteins (VLDL) remains a challenge.
•
We therefore generated apoB100-FLAG mice, which display normal lipid levels and concentration of FLAG-tagged apoB100 in plasma. However, we were unable to detect either apoB or FLAG in primary hepatocytes using immunofluorescence.
•
Blocking proteasomal degradation alone or combined with inhibition of the secretory pathway only marginally improved apoB detection.
•
Our study suggests that the physiological concentration of apoB100 in isolated hepatocytes is too low and heterogeneous to allow for studies into the subcellular localisation of apoB.

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来源期刊

Biochimica et biophysica acta. Molecular and cell biology of lipids 生物-生化与分子生物学

CiteScore

11.00

自引率

2.10%

发文量

109

审稿时长

53 days

期刊介绍： BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.