脂质成像质谱:迈向新的分子组织学。

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ibai Calvo , Olatz Fresnedo , Lorena Mosteiro , José I. López , Gorka Larrinaga , José A. Fernández
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引用次数: 0

摘要

脂质研究正受到越来越多的关注,因为这些分子是了解细胞新陈代谢以及基因型与表型之间联系的关键组成部分。脂质研究也得到了新的强大技术的推动,这些技术能够在一次运行中鉴定越来越多的物种,而且浓度越来越低。其中一项重要发展是图像技术,它能以细胞分辨率观察脂质在组织中的分布。得益于此类技术所报告的空间信息,可以将脂质体特征与处于固定代谢阶段的单个细胞联系起来,这大大有助于了解与癌症等各种病理情况相关的代谢变化。此外,脂质图像正在成为一种新的分子组织学,很有可能在医院诊断部门产生影响。在此,我们研究了该技术的现状,并分析了将其引入诊断部门的下一步措施。为了说明这项技术的潜力和挑战,我们介绍了一个关于透明细胞肾细胞癌的案例研究,由于其显著的细胞和分子异质性,透明细胞肾细胞癌是分析恶性肿瘤的良好模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lipid imaging mass spectrometry: Towards a new molecular histology
Lipid research is attracting greater attention, as these molecules are key components to understand cell metabolism and the connection between genotype and phenotype. The study of lipids has also been fueled by the development of new and powerful technologies, able to identify an increasing number of species in a single run and at decreasing concentrations. One of such key developments has been the image techniques that enable the visualization of lipid distribution over a tissue with cell resolution. Thanks to the spatial information reported by such techniques, it is possible to associate a lipidome trait to individual cells, in fixed metabolic stages, which greatly facilitates understanding the metabolic changes associated to diverse pathological conditions, such as cancer. Furthermore, the image of lipids is becoming a kind of new molecular histology that has great chances to make an impact in the diagnostic units of the hospitals. Here, we examine the current state of the technology and analyze what the next steps to bring it into the diagnosis units should be. To illustrate the potential and challenges of this technology, we present a case study on clear cell renal cell carcinoma, a good model for analyzing malignant tumors due to their significant cellular and molecular heterogeneity.
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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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