Bioanalysis最新文献_第3页

UPLC-MS/MS method for fluconazole determination in plasma and its application in Mexican patients with candidaemia. 测定血浆中氟康唑含量的 UPLC-MS/MS 方法及其在墨西哥念珠菌血症患者中的应用

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-05 DOI: 10.1080/17576180.2024.2387452

Karla Paulina Valero Rivera, Martin Magaña Aquino, Julia Sagahón Azúa, María Del Carmen Romero Méndez, Susanna Edith Medellín Garibay, Rosa Del Carmen Milán Segovia, Fidel Martínez Gutiérrez, Silvia Romano Moreno

{"title":"UPLC-MS/MS method for fluconazole determination in plasma and its application in Mexican patients with candidaemia.","authors":"Karla Paulina Valero Rivera, Martin Magaña Aquino, Julia Sagahón Azúa, María Del Carmen Romero Méndez, Susanna Edith Medellín Garibay, Rosa Del Carmen Milán Segovia, Fidel Martínez Gutiérrez, Silvia Romano Moreno","doi":"10.1080/17576180.2024.2387452","DOIUrl":"https://doi.org/10.1080/17576180.2024.2387452","url":null,"abstract":"Aim: An accurate and fast ultra-high performance liquid chromatography coupled with tandem mass spectrometry analytical method was developed and validated for quantifying fluconazole levels in human plasma according to the US FDA guidelines.Materials & methods: A simple protein precipitation by acetonitrile was employed for the sample preparation. The chromatographic separation was carried out using isocratic elution of water (0.1% formic acid) and acetonitrile on an Acquity ultra-high performance liquid chromatography HSS T3 column. Samples from ten adult patients diagnosed with candidemia who received fluconazole treatment were analyzed.Results & conclusion: The method demonstrated excellent linearity and stability within the 1-50 μg/ml range (r2 >0.999). The intraday and interday precision were determined with coefficient of variation values ranging from 1.4 to 4.38% and 2.8 to 6.6%, respectively. This rapid and selective method has successfully analyzed 27 plasma samples. The straightforward sample preparation in a single step and the reduced analysis time make this method suitable for adult patients with candidemia, leading to improved clinical outcomes.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing prognosis by quantifying FcγRIIa on fixed platelets. 通过量化固定血小板上的 FcγRIIa 评估预后。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-04 DOI: 10.1080/17576180.2024.2395706

David J Schneider, Heidi S Taatjes-Sommer, Peter M DiBattiste, Kanwal S Palla, Tyler Shovah, Subhanip Biswas, Jeanne Ohrnberger

{"title":"Assessing prognosis by quantifying FcγRIIa on fixed platelets.","authors":"David J Schneider, Heidi S Taatjes-Sommer, Peter M DiBattiste, Kanwal S Palla, Tyler Shovah, Subhanip Biswas, Jeanne Ohrnberger","doi":"10.1080/17576180.2024.2395706","DOIUrl":"https://doi.org/10.1080/17576180.2024.2395706","url":null,"abstract":"Introduction: FcγRIIa amplifies platelet activation and higher platelet FcγRIIa identifies patients at greater risk of subsequent cardiovascular events. We report the accuracy and precision of a modified test to quantify FcγRIIa on previously fixed platelets (pFCG test).Methods & results: An antibody clone (5G1) was developed after exposure of mice to formaldehyde treated FcγRIIa. Accuracy and precision of the modified test was evaluated with biologic specimens (platelets) and engineered synthetic cells conjugated with FcγRIIa (Slingshot Biosciences). The modified pFCG test on fixed platelets (using 5G1) consistently identified modestly more (∼300 molecules) of FcγRIIa on platelets compared with the pFCG test on nonfixed platelets (using clone FL18.26). With biologic specimens, the intra-assay coefficient of variation (CV) was 2.1 ± 0.1% (standard error of the mean, n = 750). The interassay CV was assessed intraday (4.5 ± 1%) and interday (up to 5 days after fixation, 6.5 ± 0.4%, n = 50). The pFCG test performed on Slingshot Synthetic cells conjugated with FcγRIIa demonstrated accuracy, linearity (R2 = 0.984) and similar interassay CV both intraday (2% ± 0.6%) and interday (20 nonconsecutive days, 9.9% ± 2.1%).Conclusion: In summary, modification of the pFCG test to be performed on fixed platelets allows accurate quantification of pFCG with high precision.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-08-09 DOI: 10.1080/17576180.2024.2391240

引用次数: 0

Evaluating the "Blueness" of a method: the new drift to assess practicality in (green) bioanalysis. 评估方法的 "蓝度"：评估（绿色）生物分析实用性的新漂移。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-07-17 DOI: 10.1080/17576180.2024.2374670

Victoria Samanidou

引用次数: 0

Role of miRNA-21 in cancer and its application in electrochemical bioanalysis. miRNA-21 在癌症中的作用及其在电化学生物分析中的应用。

IF 1.9 4区医学