Bioanalysis最新文献_第2页

Quantifying skin permeation of a novel metformin lotion using modified hydrophilic interaction liquid chromatography. 利用改良亲水相互作用液相色谱法量化新型二甲双胍乳液的皮肤渗透性。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-10-16 DOI: 10.1080/17576180.2024.2412438

Derek J Maloney, Jianying Zhang, Satyaj Bhargava, MaCalus V Hogan, James H-C Wang

{"title":"Quantifying skin permeation of a novel metformin lotion using modified hydrophilic interaction liquid chromatography.","authors":"Derek J Maloney, Jianying Zhang, Satyaj Bhargava, MaCalus V Hogan, James H-C Wang","doi":"10.1080/17576180.2024.2412438","DOIUrl":"https://doi.org/10.1080/17576180.2024.2412438","url":null,"abstract":"Aim: This study aimed to quantify the permeation of metformin (Met) lotion through pig ear skin using high-performance liquid chromatography, specifically hydrophilic interaction liquid chromatography (HILIC), to separate Met from biological contaminants and effectively measure its permeation through skin similar to human skin.Materials & methods: A Franz cell permeation assay was used to assess the permeation kinetics of 6% Met lotion through pig ear skin. Samples were collected at various time points and prepared for high-performance liquid chromatography analysis by removing large biological contaminants. The permeated Met was quantified by monitoring its retention time (RT) at 9 min using HILIC, with an acidic, polar mobile phase and a normal-phase column.Results: A distinct Met peak with a RT of approximately 9 min was observed in the 6% Met lotion, which was absent in the permeation samples from the 0% Met lotion. This peak (RT 9 min) was distinct from the 'biological-contaminants' peaks at RT 2-3 min and increased linearly over time, reaching 36.8% of the total applied Met at 24 h.Conclusion: These findings demonstrate that the HILIC method effectively separates Met from biological components in pig ear skin, allowing accurate quantification of Met despite the presence of skin lipids and proteins.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An immunoassay for the quantification of phosphorylated α-synuclein at serine 129 in human cerebrospinal fluid. 定量检测人脑脊液中丝氨酸 129 磷酸化 α-突触核蛋白的免疫测定法。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-10-15 DOI: 10.1080/17576180.2024.2407718

Achani M Silva, Elizabeth S Hickford, Paul Cutler

引用次数: 0

Development and validation of an ultra-performance liquid chromatography with tandem mass spectrometry method for determination of soluble repulsive guidance molecule A in human serum and cerebrospinal fluid. 开发并验证测定人血清和脑脊液中可溶性斥导分子 A 的超高效液相色谱-串联质谱法。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-10-10 DOI: 10.1080/17576180.2024.2403241

Patrick Schreier, Lili Huang, Emma Fung, Jennifer Mollon, Bernhard Sielaff, Marc R Lake, Michael Schulz, Khader Awwad

引用次数: 0

Assessment of mirvetuximab soravtansine immunogenicity in patients with folate receptor alpha-positive ovarian cancer. 评估叶酸受体α阳性卵巢癌患者的米尔韦妥昔单抗索拉坦辛免疫原性。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-10-08 DOI: 10.1080/17576180.2024.2407228

Fengying Zhu, Ya-Ping Tu, Callum Sloss, Yuemei Wang

{"title":"Assessment of mirvetuximab soravtansine immunogenicity in patients with folate receptor alpha-positive ovarian cancer.","authors":"Fengying Zhu, Ya-Ping Tu, Callum Sloss, Yuemei Wang","doi":"10.1080/17576180.2024.2407228","DOIUrl":"https://doi.org/10.1080/17576180.2024.2407228","url":null,"abstract":"Aim: The aim of this research was to evaluate the immunogenicity of mirvetuximab soravtansine (MIRV), an antibody-drug conjugate in patients with folate receptor alpha-positive ovarian cancer across four clinical studies.Materials & methods: An assay was developed and validated for the detection of antidrug antibodies (ADAs) against MIRV. A cell-based method was also developed and validated for the detection of neutralizing anti-MIRV antibodies (NAbs). Both ADAs and NAbs were assessed across four clinical studies in 734 patients.Results: Across studies, MIRV demonstrated low immunogenicity with 7.8% of patients with treatment-emergent ADAs, 7.2% with treatment-unaffected ADAs, and 0.5% with treatment-enhanced ADAs. MIRV trough concentrations were comparable in ADA-negative and ADA-positive individuals. Limited data suggest that MIRV ADAs may be associated with decreased efficacy. Due to the very limited number of NAb-positive individuals, no conclusions could be drawn on the effect of NAb on efficacy.Conclusion: Both the validation tests and the data from the MIRV clinical studies demonstrated that these assays were suitable and reliable for the detection of MIRV ADAs and NAbs. These validated assays will continue to be used to monitor MIRV immunogenicity in future clinical trials.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of MDR-TB drug JBD0131 and its metabolite in plasma via UPLC-MS/MS: application in first-in-human study. 通过 UPLC-MS/MS 对血浆中的 MDR-TB 药物 JBD0131 及其代谢物进行定量：在首次人体试验中的应用。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-10-07 DOI: 10.1080/17576180.2024.2404311

Tiantao Gao, Xiaoxue Ou, Jia Miao, Yongping Qin

{"title":"Quantification of MDR-TB drug JBD0131 and its metabolite in plasma via UPLC-MS/MS: application in first-in-human study.","authors":"Tiantao Gao, Xiaoxue Ou, Jia Miao, Yongping Qin","doi":"10.1080/17576180.2024.2404311","DOIUrl":"https://doi.org/10.1080/17576180.2024.2404311","url":null,"abstract":"Aim: JBD0131, a novel anti-multidrug-resistant tuberculosis (MDR-TB) drug, can target and inhibit the synthesis of mycolic acids, which are crucial components of the cell wall of the Mycobacterium tuberculosis complex. To support the results of this clinical trial in healthy subjects, development of a specific and accurate quantification method for detecting JBD0131 and its metabolite DM131 in human plasma is needed.Materials & methods: Samples with prior added stabilizer were pretreated by protein precipitation method and the extracts were subjected to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The m/z transitions for the precursor/product ion pairs were 402.1/273 for JBD0131, 333.1/273 for DM131 and 386.1/257 for the internal standard (IS).Results: This method showed good linearity from 1 to 2000 ng/ml for JBD0131 and 0.25 to 500 ng/ml for DM131 and was validated in terms of selectivity, linearity, accuracy, precision, matrix effect, recovery of pretreament and stability.Conclusion: This method was sensitive and specific for measuring the plasma concentrations of JBD0131 and its metabolites. And it was applied for the investigation of the pharmacokinetics of JBD0131 and DM131 in a clinical trial.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of drugs of abuse and metabolites in plasma microsamples by LC-MS/MS after microQuEChERS extraction. 微量 QuEChERS 萃取后通过 LC-MS/MS 测定血浆微量样本中的滥用药物和代谢物。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-27 DOI: 10.1080/17576180.2024.2404383

Mariana Smidt, Marcos Frank Bastiani, Roberta Zilles Hahn, Lilian de Lima Feltraco Lizot, Amanda Pacheco Bondan, Giovana Piva Peteffi, Náthaly Cbf Dos Santos, José Luiz da Costa, Rafael Lanaro, Rafael Linden

{"title":"Determination of drugs of abuse and metabolites in plasma microsamples by LC-MS/MS after microQuEChERS extraction.","authors":"Mariana Smidt, Marcos Frank Bastiani, Roberta Zilles Hahn, Lilian de Lima Feltraco Lizot, Amanda Pacheco Bondan, Giovana Piva Peteffi, Náthaly Cbf Dos Santos, José Luiz da Costa, Rafael Lanaro, Rafael Linden","doi":"10.1080/17576180.2024.2404383","DOIUrl":"https://doi.org/10.1080/17576180.2024.2404383","url":null,"abstract":"Aim: Identifying drugs of abuse and their metabolites in plasma is vital in both forensic and clinical toxicology. While the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method offers an efficient approach to sample preparation, its application is complex due to the wide-ranging properties of target analytes and the challenges posed by biological matrix interferences. This study aims to develop a microQuEChERS approach for the quantification of 14 drugs of abuse and metabolites utilizing minimal sample and solvent volumes.Methods: The microQuEChERS method involved using 10 μl plasma samples, 25 mg of a salt mixture and 150 μl of acetonitrile. Extracts were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), with a 7.5 min run. The assay was validated according to bioanalytical guidelines.Results: The accuracy was 96.8-112.4%. The within-assay precision was within 2.0-8.9% and the between-assay precision was within 3.2-8.2%. Matrix effects were found to range from -5.7 to 13.5%. The extraction yield was higher than 74.7%.Conclusion: This study described a microQuEChERS sample preparation approach for determining drugs of abuse and metabolites using plasma microsamples and LC-MS/MS. The approach is efficient, environmentally friendly and suitable for scenarios with limited amounts of biological samples.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-residue analysis method based on QuEChERS followed by ultra-high performance liquid chromatography coupled with diode-array detector for pesticides in human serum and breast milk. 基于 QuEChERS 的多残基分析方法，然后采用超高效液相色谱法和二极管阵列检测器检测人血清和母乳中的农药。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-25 DOI: 10.1080/17576180.2024.2403206

Prapha Arnnok, Rodjana Burakham

引用次数: 0

High-performance liquid chromatography-tandem mass spectrometry was used to measure 20 antidepressants in human serum. 采用高效液相色谱-串联质谱法测定了人体血清中的 20 种抗抑郁药物。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-24 DOI: 10.1080/17576180.2024.2401283

Huanchang Luo, Jianwei Zhou, Guanfeng Lin, Xiaojing Huang, Yuhang Yang, Yingsong Wu

{"title":"High-performance liquid chromatography-tandem mass spectrometry was used to measure 20 antidepressants in human serum.","authors":"Huanchang Luo, Jianwei Zhou, Guanfeng Lin, Xiaojing Huang, Yuhang Yang, Yingsong Wu","doi":"10.1080/17576180.2024.2401283","DOIUrl":"10.1080/17576180.2024.2401283","url":null,"abstract":"Aim: This study used high performance liquid chromatography-tandem mass spectrometry to quantify the blood concentrations of 20 antidepressants, such as bupropion and fluoxetine, in human serum samples.Methods: After direct precipitation with a 1:9 protein precipitant of methanol and acetonitrile, serum samples were examined using high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS). The material was separated using a Poroshell 120 EC-C18 column (3.0 mm × 50 mm, 2.7 μm) and gradient elution. The mobile phases were phase A 0.01% formic acid aqueous solution (containing 2 mmol/ml ammonium acetate) and phase B methanol solution. A 0.45 ml/min flow rate was used to divide the sample and inject 5 μl. Electrospray ionization source positive ion mode and multiple reaction monitoring modes were used for analysis. Measurement was quantified using an internal standard technique.Results: Accuracy ranged from 90.3 to 114.3%, intra-day precision from 100.1 to 112.3%, inter-day precision from 100.4 to 112.6%, extraction recoveries from 85.5 to 114.5% and matrix effect from 85.6 to 98.7%.Conclusion: This approach is fast, accurate, sensitive and repeatable. It can identify 20 antidepressants in blood simultaneously. This can be used to monitor blood drug levels and medication metabolism.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highlights of the 15th Japan Bioanalysis Forum Symposium. 第 15 届日本生物分析论坛研讨会要点。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-12 DOI: 10.1080/17576180.2024.2395715

Makoto Niwa, Tomoko Arakawa, Koji Arai, Noriyuki Koyama, Hisanori Hara, Katsutomo Hata, Masayo Hashimoto, Eiichi Shimada, Hiroyuki Shimizu, Yuki Takamatsu, Hitoshi Uchiyama, Takeru Yamaguchi, Kenichi Yamamoto, Masaaki Kakehi, Yuu Moriya, Hironosuke Kurisu, Hisao Nagaya, Tsunenori Nakazawa, Shohei Noguchi, Yuki Oda, Hiromi Okamoto, Shinsuke Uchihashi

引用次数: 0

Interference from anti-drug antibodies on the quantification of insulin: a comparison of an LC-MS/MS assay and immunoassays. 抗药性抗体对胰岛素定量的干扰：LC-MS/MS 检测法与免疫测定法的比较。

IF 1.9 4区医学

Bioanalysis Pub Date : 2024-09-12 DOI: 10.1080/17576180.2024.2389637

Georgina Marta Varga, Manca Spendal, Jens Sigh, Tue Søeborg, Nikoline Juul Nielsen

引用次数: 0