口服氢溴化山莨菪片对大鼠生理及脑缺血再灌注损伤的药动学-药效学及组织分布研究。

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-08-30 DOI:10.1080/17576180.2025.2554567

Yujie Yu, Yanfang Liu, Jianlan Zhang, Shu Dai, Rui Wu, Feng Wan, Chenhao Yao, Yuxin Yao, Feng Nan, Yunxia Li

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引用次数: 0

摘要

目的：建立快速、灵敏的高效液相色谱-串联质谱（UPLC-MS/MS）分析口服氢溴山莨菪碱（AH）片药动学的方法。方法：比较两组大鼠给药剂量分别为5、10、20 mg/kg的AH（体内以山莨菪碱形式存在）及其代谢物NOAT3的药代动力学差异。此外，以血药浓度为药动学参数，以白细胞介素-1β (IL-1β)和乳酸脱氢酶（LDH）为药理学指标，建立生理模型大鼠和CIRI模型大鼠药动学-药动力学（PK-PD）联合模型，分析AH对CIRI的保护作用。结果与结论：本研究表明，AH可迅速、广泛地分布于大鼠的各组织中。AH是一种很有前景的治疗大鼠CIRI模型的药物。

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Pharmacokinetic-pharmacodynamic and tissue distribution studies in physiological and cerebral ischemia-reperfusion injury rats after oral administration of anisodine hydrobromide tablets.

Aim: We aim to establish a rapid and sensitive UPLC-MS/MS method to analyze the pharmacokinetics of oral anisodine hydrobromide (AH) tablets.

Methods: Comparison of pharmacokinetic differences between AH (present in vivo as anisodine) and its metabolite NOAT3 in two groups of rats after administration of AH at doses of 5, 10, and 20 mg/kg. In addition, plasma concentrations of AH were used as a pharmacokinetic parameter, and interleukin-1β (IL-1β) and lactic dehydrogenase (LDH) were selected as pharmacodynamic indicators to establish a pharmacokinetics-pharmacodynamics (PK-PD) combined model in physiological and CIRI model rats, to analyze the protective effect of AH against CIRI.

Results & conclusion: The study demonstrated that AH could be rapidly and extensively distributed to various tissues in rats. AH is a promising drug for the treatment of rat models of CIRI.

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.