Evaluating the efficacy of the VAMS Mitra microsampler for whole blood trace element analysis.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-08-22 DOI:10.1080/17576180.2025.2549239

Oludesola Ogunesan, Christa Dahman Zaborske, Cassandra Newsom, Martin M Shafer, Kristina M Zierold, Jeffrey K Wickliffe

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引用次数: 0

Abstract

Background: Volumetric Absorptive Microsampling (VAMS) is a blood sample collection method proposed as an alternative to venipuncture for metals/elements biomonitoring. However, the microsampler background concentration of metals and small blood volume remains critical limitations, particularly for metals or trace element analysis in environmental health and epidemiological research.

Materials & methods: Trace element analysis was performed to measure metal concentration in blood samples collected via VAMS and venipuncture using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). VAMS blanks showed elevated background concentration, and cleaning was attempted using a multi-step cleaning protocol. Detection limits and efficacy of background concentration reduction were evaluated.

Results: Initial analysis showed elevated background metal concentrations in the VAMS blank samplers, at or exceeding levels found in venous blood samples. VAMS blank with background concentration post-cleaning result indicated reductions in concentration for some metals; however, the concentration for most detected metals remained persistent.

Conclusions: The efficacy of VAMS in environmental health and epidemiological biomarker research demonstrates both the promising potential and limitations, and the effectiveness of a rigorous cleaning protocol in reducing or eliminating background metal concentrations on microsampler tips.

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评价VAMS Mitra微采样器用于全血微量元素分析的有效性。

背景：体积吸收微采样（VAMS）是一种血液样本采集方法，被提议作为静脉穿刺金属/元素生物监测的替代方法。然而，金属的微采样器背景浓度和小血容量仍然是关键的限制，特别是在环境卫生和流行病学研究中的金属或微量元素分析方面。材料与方法：微量元素分析采用电感耦合等离子体质谱（ICP-MS）测定VAMS和静脉穿刺采集的血液样品中的金属浓度。VAMS空白显示背景浓度升高，并尝试使用多步骤清洗方案进行清洗。评价了本底浓度降低的检出限和效果。结果：初步分析显示，VAMS空白样本中的背景金属浓度升高，达到或超过静脉血样本中的水平。VAMS空白与背景浓度清洗后的结果表明，一些金属的浓度降低；然而，大多数检测到的金属的浓度仍然持续存在。结论：VAMS在环境健康和流行病学生物标志物研究中的有效性显示了其良好的潜力和局限性，以及严格的清洁方案在降低或消除微采样器尖端的背景金属浓度方面的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.