Effect of delayed blood centrifugation on cytokine quantitation in serum and plasma.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-07-01 Epub Date: 2025-08-12 DOI:10.1080/17576180.2025.2544521

Xiaoyun Yang, Trinidad Arceo, Saloumeh K Fischer

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引用次数: 0

Abstract

Background: Cytokines are critical biomarkers, but their accurate measurement is susceptible to pre-analytical variables. This study investigated the effect of delayed blood processing on the quantitation of eight cytokines (CCL2, CXCL10, IFN-γ, IL-2Ra, IL-6, IL-7, IL-18, TNFα) in matched serum and plasma from healthy volunteers. Methods: Blood was processed immediately or after 3, 6, 24, and 48 hours, stored at room temperature or 4°C. Cytokines were quantified using a multiplexed Ella assay.

Results: Our data showed only IL-2Ra remained stable across all conditions. The remaining cytokine concentrations were significantly impacted by processing delay and storage temperature, with changes evident as early as 3 hours and more pronounced at 24 and 48 hours (p < 0.05). Storing blood at 4°C generally mitigated changes compared to room temperature, but analyte-specific and matrix-dependent variations persisted.

Conclusion: These findings emphasize delayed processing significantly alters cytokine levels, highlighting the importance of prompt, standardized processing and careful consideration of pre-analytical factors for reliable cytokine quantitation in clinical studies.

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延迟血液离心对血清和血浆细胞因子定量的影响。

背景：细胞因子是重要的生物标志物，但其准确测量容易受到分析前变量的影响。本研究探讨了延迟血液处理对健康志愿者匹配血清和血浆中8种细胞因子（CCL2、CXCL10、IFN-γ、IL-2Ra、IL-6、IL-7、IL-18、TNFα）定量的影响。方法：立即或3、6、24、48 h后处理血液，室温或4℃保存。细胞因子定量使用多重Ella试验。结果：我们的数据显示，在所有条件下，只有IL-2Ra保持稳定。剩余的细胞因子浓度受加工延迟和储存温度的显著影响，变化早在3小时就明显，在24和48小时时更为明显(p结论：这些发现强调延迟加工显著改变细胞因子水平，强调及时、标准化处理和仔细考虑分析前因素对临床研究中可靠的细胞因子定量的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.