Biochemistry Insights最新文献_第5页

Enzymatic Synthesis and Anti-Allergic Activities of Curcumin Oligosaccharides 姜黄素低聚糖的酶促合成及其抗过敏活性研究

Biochemistry Insights Pub Date : 2010-01-01 DOI: 10.4137/BCI.S2768

K. Shimoda, H. Hamada

引用次数: 12

Synthesis of Capsaicin Glycosides and 8-Nordihydrocapsaicin Glycosides as Potential Weight-Loss Formulations 辣椒素糖苷和8-去甲二氢辣椒素糖苷作为潜在减肥制剂的合成

Biochemistry Insights Pub Date : 2010-01-01 DOI: 10.4137/BCI.S2676

H. Katsuragi, K. Shimoda, E. Kimura, H. Hamada

引用次数: 3

Preparation of Chiral 2-chloromandelamide: Stereoselective Reduction of an Aromatic α-keto Amide with Actinomycete Strains 手性2-氯曼酰胺的制备:放线菌立体选择性还原芳族α-酮酰胺

Biochemistry Insights Pub Date : 2010-01-01 DOI: 10.4137/BCI.S4231

K. Ishihara, M. Nishimura, K. Nakashima, Noriko Machii, F. Miyake, M. Nishi, Momoko Yoshida, N. Masuoka, N. Nakajima

{"title":"Preparation of Chiral 2-chloromandelamide: Stereoselective Reduction of an Aromatic α-keto Amide with Actinomycete Strains","authors":"K. Ishihara, M. Nishimura, K. Nakashima, Noriko Machii, F. Miyake, M. Nishi, Momoko Yoshida, N. Masuoka, N. Nakajima","doi":"10.4137/BCI.S4231","DOIUrl":"https://doi.org/10.4137/BCI.S4231","url":null,"abstract":"The stereoselective reduction of an aromatic α-keto amide with actinomycete strains was investigated. It was found that 2-chlorobenzoylformamide was reduced to the corresponding 2-chloromandelamide by mesophilic and thermophilic strains of actinomycetes. Among the strains tested, the reduction of 2-chlorobenzoylformamide by Streptomyces thermocyaneoviolaceus (one of thermophilic strains) in the presence of glycerol as an additive produced only (S)-2-chloromandelamide in >99% conversion with >99% enantiomeric excess (e.e.). On the other hand, the reduction by Streptomyces thermocarboxydovorans NBRC16324 at 45 °C or Thermoactinomyces vulgaris NBRC15851 cultivated in a soluble starch-based medium gave the corresponding (R)-hydroxy amide (conversion, 99%; >99% e.e.). Mesophilic and other thermophilic actinomycete strains also catalyzed the reduction to the corresponding (R)-hydroxy amide with 85%–>99% e.e. Thus, the syntheses of both enantiomers of 2-chloromandelamide was achieved though the reduction of 2-chlorobenzoylformamide with different actinomycete strains.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S4231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70693742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Collagen Racemization and Deposition in the Lungs of Aged Rats 老年大鼠肺内胶原消旋化及沉积

Biochemistry Insights Pub Date : 2010-01-01 DOI: 10.4137/BCI.S4210

A. Kasai, N. Yamashita, N. Utsunomiya‐Tate

{"title":"Collagen Racemization and Deposition in the Lungs of Aged Rats","authors":"A. Kasai, N. Yamashita, N. Utsunomiya‐Tate","doi":"10.4137/BCI.S4210","DOIUrl":"https://doi.org/10.4137/BCI.S4210","url":null,"abstract":"Isomerization of amino acids in proteins has recently been identified as a part of the aging process. Increases in D-amino acids as a consequence of isomerization influence the function and structure of proteins. Senescence-related pulmonary diseases, such as chronic obstructive pulmonary disease, are thought to be caused by reductions of lung function with age. We hypothesized that changes of protein structure in lung tissue induced by the isomerization of amino acids could result in decreased lung function. Therefore, we examined whether isomerization of amino acids takes place in the lungs of rats as they age. We measured the content of L- and D-amino acids in collagen 1 by HPLC using a chiral column. We found that collagen 1 was increasingly racemized with age, so that significantly higher proportions of D-Ser were present in 12- and 24-month-old rats than in 8-week-old rats. D-Asp increased slightly but not significantly. We also investigated the localization of collagen 1 in lung tissue. Stacks of collagen 1 were observed in the parenchyma and airway wall, and age-dependent changes were especially prominent in the airway wall. Racemization of collagen 1 could therefore influence lung function and contribute to pulmonary diseases.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S4210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70694094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli 活性牛末端脱氧核苷酸转移酶(TdT)在大肠杆菌中的产生

Biochemistry Insights Pub Date : 2010-01-01 DOI: 10.4137/BCI.S5123

Wee Liang Kuan, J. Joy, Ng Fui Mee, Kwek Zekui Perlyn, Then Siew Wen, T. Nguen, Joanne James, E. Chai, H. Flotow, Sharon C. Crasta, Kelvin Chua, Ng Sok Peng, Jeffrey Hill

引用次数: 0

Article Commentary: Chelation Therapy for Mercury Poisoning 文章评论:汞中毒的螯合治疗

Biochemistry Insights Pub Date : 2009-01-01 DOI: 10.4137/BCI.S3036

R. Guan, H. Dai

引用次数: 2

On Message Ribonucleic Acids Targeting to Mitochondria 靶向线粒体的信息核糖核酸

Biochemistry Insights Pub Date : 2009-01-01 DOI: 10.4137/BCI.S3745

Di Ding, K. Dave, S. Bhattacharya

引用次数: 3

Production of (2R,3S)-2-Benzamidomethyl-3-Hydroxybutanoates by Immobilized Plant Cells of Parthenocissus Tricuspidata 固定化孤女植物细胞生产(2R,3S)-2-苄基甲基-3-羟基丁酸盐

Biochemistry Insights Pub Date : 2009-01-01 DOI: 10.4137/BCI.S961

K. Shimoda, N. Kubota, H. Hamada, Tatsunari Kobayashi, H. Hamada, S. M. Shafi, N. Nakajima

引用次数: 4

Article Commentary: A Role for IR-β in the Free Fatty Acid Mediated Development of Hepatic Insulin Resistance? 文章评论:IR-β在游离脂肪酸介导的肝脏胰岛素抵抗中的作用?

Biochemistry Insights Pub Date : 2009-01-01 DOI: 10.4137/BCI.S2996

Samit Shah, A. Cox

引用次数: 0

A Novel Mechanism in Regulating the Alpha-Subunit of the Epithelial Sodium Channel (α ENaC) by the Alternatively Spliced Form α ENaC-b α ENaC-b选择性剪接调控上皮钠通道α亚基的新机制

Biochemistry Insights Pub Date : 2009-01-01 DOI: 10.4137/BCI.S880

M. Shehata

{"title":"A Novel Mechanism in Regulating the Alpha-Subunit of the Epithelial Sodium Channel (α ENaC) by the Alternatively Spliced Form α ENaC-b","authors":"M. Shehata","doi":"10.4137/BCI.S880","DOIUrl":"https://doi.org/10.4137/BCI.S880","url":null,"abstract":"Introduction In Dahl rats’ kidney cortex, the alternatively spliced form of the epithelial sodium channel α subunit (α ENaC-b) is the most abundant mRNA transcript (32+/-3 fold > α ENaC-wt) as was investigated by quantitative RT-PCR analysis. α ENaC-b mRNA levels were significantly higher in Dahl R versus S rats, and were further augmented by high salt diet. Objectives In the present study, we described the molecular cloning and searched for a possible role of α ENaC-b by testing its potential expression in COS7 cells as well as its impact on α ENaC-wt expression levels when co-expressed in COS7 cells in a dose-dependent manner. Methods Using RT-PCR strategy, the full-length wildtype α ENaC transcript and the alternatively spliced form α ENaC-b were amplified, sequenced, cloned, subcloned into PCMV-sport6 expression vector, expressed and co-expressed into COS7 cells in a dose-dependent manner. A combination of denaturing and native western blotting techniques was employed to examine the expression of α ENaC-b in vitro, and to determine if an interaction between α ENaC-b and α ENaC-wt occurs in vitro, and finally to demonstrate if degradation of α ENaC-wt protein does occur. Results α ENaC-b is translated in COS7 cells. Co-expression of α ENaC-b together with α ENaC-wt reduced α ENaC-wt levels in a dose-dependent manner. α ENaC-wt and α ENaC-b appear to form a complex that enhances the degradation of α ENaC-wt. Conclusions Western blots suggest a novel mechanism in α ENaC regulation whereby α ENaC-b exerts a dominant negative effect on α ENaC-wt expression. This is potentially by sequestering α ENaC-wt, enhancing its proteolytic degradation, and possibly explaining the mechanism of salt-resistance in Dahl R rats.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70685214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2