Wee Liang Kuan, J. Joy, Ng Fui Mee, Kwek Zekui Perlyn, Then Siew Wen, T. Nguen, Joanne James, E. Chai, H. Flotow, Sharon C. Crasta, Kelvin Chua, Ng Sok Peng, Jeffrey Hill
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Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli
A synthetic gene encoding bovine terminal deoxynucleotidyl transferase (TdT) was generated, cloned into an expression vector and expressed in E.coli. The effects of altering culture and induction conditions on the nature of recombinant protein production were investigated. This led to the expression of active recombinant bovine TdT in E.coli. After purification and characterisation, the activity of the enzyme was assessed in a biological assay for apoptosis. The process described in this report enables the economical production of TdT for high throughput applications.
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