Biochemistry Insights最新文献_第4页

Plant-based Paste Fermented by Lactic Acid Bacteria and Yeast: Functional Analysis and Possibility of Application to Functional Foods. 乳酸菌和酵母发酵植物膏的功能分析及其在功能食品中的应用

Biochemistry Insights Pub Date : 2012-10-31 eCollection Date: 2012-01-01 DOI: 10.4137/BCI.S10529

Shinsuke Kuwaki, Nobuyoshi Nakajima, Hidehiko Tanaka, Kohji Ishihara

{"title":"Plant-based Paste Fermented by Lactic Acid Bacteria and Yeast: Functional Analysis and Possibility of Application to Functional Foods.","authors":"Shinsuke Kuwaki, Nobuyoshi Nakajima, Hidehiko Tanaka, Kohji Ishihara","doi":"10.4137/BCI.S10529","DOIUrl":"https://doi.org/10.4137/BCI.S10529","url":null,"abstract":"A plant-based paste fermented by lactic acid bacteria and yeast (fermented paste) was made from various plant materials. The paste was made of fermented food by applying traditional food-preservation techniques, that is, fermentation and sugaring. The fermented paste contained major nutrients (carbohydrates, proteins, and lipids), 18 kinds of amino acids, and vitamins (vitamin A, B1, B2, B6, B12, E, K, niacin, biotin, pantothenic acid, and folic acid). It contained five kinds of organic acids, and a large amount of dietary fiber and plant phytochemicals. Sucrose from brown sugar, used as a material, was completely resolved into glucose and fructose. Some physiological functions of the fermented paste were examined in vitro. It was demonstrated that the paste possessed antioxidant, antihypertensive, antibacterial, anti-inflammatory, anti-allergy and anti-tyrosinase activities in vitro. It was thought that the fermented paste would be a helpful functional food with various nutrients to help prevent lifestyle diseases. ","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"5 ","pages":"21-9"},"PeriodicalIF":0.0,"publicationDate":"2012-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S10529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32578021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Chemo-enzymatic synthesis of ester-linked 2-phenylindole-3-carboxaldehyde-monosaccharide conjugate as potential prodrug. 作为潜在前药的酯连接的2-苯基吲哚-3-羧醛-单糖缀合物的化学酶合成。

Biochemistry Insights Pub Date : 2012-08-27 eCollection Date: 2012-01-01 DOI: 10.4137/BCI.S9961

Kei Shimoda, Manabu Hamada, Hiroshi Yokoi, Hiroki Hamada

引用次数: 1

Chemo-enzymatic synthesis of glycolyl-ester-linked taxol-monosaccharide conjugate and its drug delivery system using hepatitis B virus envelope L bio-nanocapsules. 乙型肝炎病毒包膜L型生物纳米胶囊的化学酶合成及其给药系统。

Biochemistry Insights Pub Date : 2012-07-09 eCollection Date: 2012-01-01 DOI: 10.4137/BCI.S9824

Kei Shimoda, Manabu Hamada, Masaharu Seno, Tadakatsu Mandai, Hiroki Hamada

引用次数: 1

Dying for Good: Virus-Bacterium Biofilm Co-evolution Enhances Environmental Fitness. 为善而死:病毒-细菌生物膜共同进化增强环境适应性。

Biochemistry Insights Pub Date : 2012-07-03 eCollection Date: 2012-01-01 DOI: 10.4137/BCI.S9553

Hongjun Jin, Thomas C Squier, Philip E Long

{"title":"Dying for Good: Virus-Bacterium Biofilm Co-evolution Enhances Environmental Fitness.","authors":"Hongjun Jin, Thomas C Squier, Philip E Long","doi":"10.4137/BCI.S9553","DOIUrl":"https://doi.org/10.4137/BCI.S9553","url":null,"abstract":"Commonly used in biotechnology applications, filamentous M13 phage are non-lytic viruses that infect E. coli and other bacteria, with the potential to promote horizontal gene transfer in natural populations with synthetic biology implications for engineering community systems. Using the E. coli strain TG1, we have investigated how a selective pressure involving elevated levels of toxic chromate, mimicking that found in some superfund sites, alters population dynamics following infection with either wild-type M13 phage or an M13-phage encoding a chromate reductase (Gh-ChrR) capable of the reductive immobilization of chromate (ie, M13-phageGh-ChrR). In the absence of a selective pressure, M13-phage infection results in a reduction in bacterial growth rate; in comparison, in the presence of chromate there are substantial increases in both cellular killing and biomass formation following infection of E. coli strain TG1with M13-phageGh-ChrR that is dependent on chromate-reductase activity. These results are discussed in terms of community structures that facilitate lateral gene transfer of beneficial traits that enhance phage replication, infectivity, and stability against environmental change. ","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"5 ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2012-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S9553","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32578018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Bioremediation of Benzophenone by Glycosylation with Immobilized Marine Microalga Chrysocampanulla spinifera and Amphidinium crassum 固定化海洋微藻金钟藻和沙蚌糖基化生物修复二苯甲酮

Biochemistry Insights Pub Date : 2011-01-01 DOI: 10.4137/BCI.S8212

K. Shimoda, Yushi Uchimura, Hiroya Imai, M. Kitagawa, H. Hirano, H. Hamada, H. Hamada

引用次数: 1

Glycosylation of Capsaicin Derivatives and Phenylpropanoid Derivatives Using Cultured Plant Cells 辣椒素衍生物和苯丙类衍生物在植物细胞中的糖基化

Biochemistry Insights Pub Date : 2011-01-01 DOI: 10.4137/BCI.S6682

H. Katsuragi, K. Shimoda, R. Yamamoto, K. Ishihara, H. Hamada

{"title":"Glycosylation of Capsaicin Derivatives and Phenylpropanoid Derivatives Using Cultured Plant Cells","authors":"H. Katsuragi, K. Shimoda, R. Yamamoto, K. Ishihara, H. Hamada","doi":"10.4137/BCI.S6682","DOIUrl":"https://doi.org/10.4137/BCI.S6682","url":null,"abstract":"Biotransformations of capsaicinoids such as capsaicin and 8-nordihydrocapsaicin and phenylpropanoids such as cinnamic acid, p-coumaric acid, caffeic acid, and ferulic acid have been investigated using cultured plant cells. Capsain and 8-nordihydrocapsaicin were converted into the corresponding glycosides which are three glycosides respectively using the cultured cells of Catharanthus roseus. In a time-course study under sterile conditions, the changes in amounts of their reaction products were determined. Furthermore phenypropanoid, such as cinnamic acid, p-coumaric acid, caffeic acid and ferulic acid have been biotransformed using the cultured cells of the Eucalyptus perriniana, and then cinnamic acid was converted into two glycosides. In addition, p-coumaric acid, caffeic acid and ferulic acid were converted into four, four and three glycosides respectively. Then in time-course study under sterile conditions, the change in amounts of their reaction products were determined. Finally it was found that the cultured plant cells have the ability to glycosylate the phenolic group of capsacinoids and phenylpropanoids regioselectively.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S6682","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70694103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Stereoselective Reduction of α-Keto Ester and α-Keto Amide with Marine Actinomycetes, Salinispora strains, as Novel Biocatalysts 新型生物催化剂海洋放线菌对α-酮酯和α-酮酰胺的立体选择性还原

Biochemistry Insights Pub Date : 2011-01-01 DOI: 10.4137/BCI.S7877

K. Ishihara, H. Nagai, Kazunari Takahashi, M. Nishiyama, N. Nakajima

引用次数: 8

Persistent Inflammatory Pathways Associated with Early Onset Myocardial Infarction in a Medicated Multiethnic Hawaiian Cohort 持续炎症途径与早发性心肌梗死相关的多种族用药夏威夷队列

Biochemistry Insights Pub Date : 2011-01-01 DOI: 10.4137/BCI.S6976

K. M. Szauter, M. Jansen, Gordon Okimoto, Michael Loomis, J. Kimura, M. Heller, Tercia L Ku, M. Tiirikainen, C. Boyd, K. Csiszȧr, R. Girton

{"title":"Persistent Inflammatory Pathways Associated with Early Onset Myocardial Infarction in a Medicated Multiethnic Hawaiian Cohort","authors":"K. M. Szauter, M. Jansen, Gordon Okimoto, Michael Loomis, J. Kimura, M. Heller, Tercia L Ku, M. Tiirikainen, C. Boyd, K. Csiszȧr, R. Girton","doi":"10.4137/BCI.S6976","DOIUrl":"https://doi.org/10.4137/BCI.S6976","url":null,"abstract":"In spite of current standard therapies to target the major pathomechanisms in myocardial infarction (MI), inflammatory gene expression patterns have been consistently revealed in MI patients. In a multiethnic cohort, we aimed to identify MI-associated pathomechanisms that may be unresponsive to medical treatment to improve diagnosis and therapy. Gene expression profiles in whole blood were analyzed in medicated Asian, African American and Caucasian patients living in Hawaii with a history of early MI and age, ethnicity, risk factor and medication-matched controls. PANTHER ontological and Ingenuity Pathway analysis and functional evaluation of the consistently differentially expressed genes identified coordinated up-regulation of genes for inflammation (LGALS3, PTX3, ZBTB32, BCL2L1), T-cell activation (IL12RB1, VAV3, JAG1, CAMP), immune imbalance (IL-8, IL2RA, CCR7, AHNAK), and active atherosclerosis (NR1H4, BIN1, GSTT1, MARCO) that persist in MI patients in spite of concerted treatment efforts to control vascular pathology. Furthermore, significant ethnic differences appear to exist within the active disease mechanisms that need to be further investigated to identify key targets for effective medical intervention.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S6976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70685230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Morphological Changes and Immunohistochemical Expression of RAGE and its Ligands in the Sciatic Nerve of Hyperglycemic Pig (Sus Scrofa). 高血糖猪坐骨神经RAGE及其配体的形态变化及免疫组织化学表达。

Biochemistry Insights Pub Date : 2010-09-01 DOI: 10.4137/BCI.S5340

Judyta K Juranek, Alexey Aleshin, Eileen M Rattigan, Lynne Johnson, Wu Qu, Fei Song, Radha Ananthakrishnan, Nosirudeen Quadri, Shi Du Yan, Ravichandran Ramasamy, Ann Marie Schmidt, Matthew S Geddis

{"title":"Morphological Changes and Immunohistochemical Expression of RAGE and its Ligands in the Sciatic Nerve of Hyperglycemic Pig (Sus Scrofa).","authors":"Judyta K Juranek, Alexey Aleshin, Eileen M Rattigan, Lynne Johnson, Wu Qu, Fei Song, Radha Ananthakrishnan, Nosirudeen Quadri, Shi Du Yan, Ravichandran Ramasamy, Ann Marie Schmidt, Matthew S Geddis","doi":"10.4137/BCI.S5340","DOIUrl":"https://doi.org/10.4137/BCI.S5340","url":null,"abstract":"The aim of our project was to study the effect of streptozotocin (STZ)-induced hyperglycemia on sciatic nerve morphology, blood plasma markers and immunohistochemical expression of RAGE (the Receptor for Advanced Glycation End-products), and its ligands-S100B and Carboxymethyl Lysine (CML)-advanced glycation endproduct (AGE) in the laboratory pig. Six months after STZ-injections, blood plasma measurements, morphometric analysis of sciatic nerve fiber density, immunofluorescent distribution of potential molecular neuropathy contributors, ELISA measurement of plasma AGE level and HPLC analysis of sciatic nerve levels of one of the pre-AGE and the glycolysis intermediate products-methyl-glyoxal (MG) were performed. The results of our study revealed that STZ-injected animals displayed elevated levels of plasma glucose, gamma glutamyl transferase (GGT) and triglycerides. The sciatic nerve of STZ-injected pigs revealed significantly lower numbers of small-diameter myelinated fibers, higher immunoreactivity for RAGE and S100B and increased levels of MG as compared to control animals. Our results correspond to clinical findings in human patients with hyperglycemia/diabetes-evoked peripheral neuropathy and suggest that the domestic pig may be a suitable large animal model for the study of mechanisms underlying hyperglycemia-induced neurological complications in the peripheral nerve and may serve as a relevant model for the pre-clinical assessment of candidate drugs in neuropathy.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"2010 3","pages":"47-59"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S5340","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30086495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

The Use of Soluble Transferrin Receptor in the Detection of rHuEPO abuse in Sports 应用可溶性转铁蛋白受体检测运动中rHuEPO滥用

Biochemistry Insights Pub Date : 2010-01-01 DOI: 10.4137/BCI.S3943

D. McGrowder, P. Brown, R. Alexander-Lindo, Shirley Budall, R. Irving, L. Gordon

{"title":"The Use of Soluble Transferrin Receptor in the Detection of rHuEPO abuse in Sports","authors":"D. McGrowder, P. Brown, R. Alexander-Lindo, Shirley Budall, R. Irving, L. Gordon","doi":"10.4137/BCI.S3943","DOIUrl":"https://doi.org/10.4137/BCI.S3943","url":null,"abstract":"Erythropoietin (EPO) increases the number of circulating erythrocytes and muscle oxygenation. The recombinant forms of EPO have indiscriminately been used by athletes, mainly in endurance sports to increase their erythrocytes concentration, thus generating a better delivery of oxygen to the muscle tissue. The administration of recombinant human erythropoietin (rHuEPO) except for therapeutic use was prohibited by the International Olympic Committee (IOC) and its unauthorized use considered as doping. In the last few years, a number of studies using parameters indicative of accelerated erythropoiesis have investigated a number of indirect methods for the detection of rHuEPO abuse. No single indirect marker has been found that can satisfactorily demonstrated rHuEPO misuse. Soluble transferrin receptor (sTfR) is a new marker of iron status and erythropoietic activity. It has been included in multivariable blood testing models for the detection of performance enhancing EPO abuse in sports. Indirect markers of altered erythropoiesis give reliable evidence of current or discontinued rHuEPO usage. This review describes the physical, biological and pharmacokinetic properties of endogenous EPO and its recombinant form. It also discusses the available strategies for the detection of rHuEPO abuse in sports, involving the use of sTfR concentration directly or in mathematical multivariate models.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S3943","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70693920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1