Biochemistry Insights最新文献_第3页

Combined Efficacy of Gallic Acid and MiADMSA with Limited Beneficial Effects Over MiADMSA Against Arsenic-induced Oxidative Stress in Mouse. 没食子酸与MiADMSA联合抗砷诱导小鼠氧化应激作用及对MiADMSA的有限有益作用

Biochemistry Insights Pub Date : 2015-08-26 eCollection Date: 2015-01-01 DOI: 10.4137/BCI.S30505

Vidhu Pachauri, Sjs Flora

{"title":"Combined Efficacy of Gallic Acid and MiADMSA with Limited Beneficial Effects Over MiADMSA Against Arsenic-induced Oxidative Stress in Mouse.","authors":"Vidhu Pachauri, Sjs Flora","doi":"10.4137/BCI.S30505","DOIUrl":"https://doi.org/10.4137/BCI.S30505","url":null,"abstract":"Gallic acid is an organic acid known for its antioxidant and anticancer properties. The present study is focused on evaluating the role of gallic acid in providing better therapeutic outcomes against arsenic-induced toxicity. Animals pre-exposed to arsenic were treated with monoisoamyl meso-2,3-dimercaptosuccinic acid (MiADMSA), a new chelating drug, alone and in combination with gallic acid, consecutively for 10 days. The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic. Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA. We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study. ","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S30505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34147767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System. 水/有机双相体系中红球菌胆固醇氧化酶产胆-4-烯-3-酮的制备、纯化和鉴定

Biochemistry Insights Pub Date : 2015-02-16 eCollection Date: 2015-01-01 DOI: 10.4137/BCI.S21580

Ke Wu, Wei Li, Jianrui Song, Tao Li

{"title":"Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System.","authors":"Ke Wu, Wei Li, Jianrui Song, Tao Li","doi":"10.4137/BCI.S21580","DOIUrl":"https://doi.org/10.4137/BCI.S21580","url":null,"abstract":"Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD) using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus) instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product. ","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S21580","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33422962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves 用组织培养的甜菊叶鉴定甜菊苷

Biochemistry Insights Pub Date : 2015-01-01 DOI: 10.4137/BCI.S30378

M. Karim, Daisuke Uesugi, Noriyuki Nakayama, M. M. Hossain, K. Ishihara, H. Hamada

引用次数: 7

Carbamylated Erythropoietin: A Prospective Drug Candidate for Neuroprotection 氨甲酰化促红细胞生成素:一种神经保护的前瞻性候选药物

Biochemistry Insights Pub Date : 2015-01-01 DOI: 10.4137/BCI.S30753

Jianmin Chen, Zhengqian Yang, Xiao Zhang

引用次数: 33

A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments 监测不同细胞区室中病毒蛋白水解裂解的单细胞平台

Biochemistry Insights Pub Date : 2015-01-01 DOI: 10.4137/BCI.S30379

Darin Abbadessa, Cameron A. Smurthwaite, Connor Reed, R. Wolkowicz

{"title":"A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments","authors":"Darin Abbadessa, Cameron A. Smurthwaite, Connor Reed, R. Wolkowicz","doi":"10.4137/BCI.S30379","DOIUrl":"https://doi.org/10.4137/BCI.S30379","url":null,"abstract":"Infectious diseases affect human health despite advances in biomedical research and drug discovery. Among these, viruses are especially difficult to tackle due to the sudden transfer from animals to humans, high mutational rates, resistance to current treatments, and the intricacies of their molecular interactions with the host. As an example of these interactions, we describe a cell-based approach to monitor specific proteolytic events executed by either the viral-encoded protease or by host proteins on the virus. We then emphasize the significance of examining proteolysis within the subcellular compartment where cleavage occurs naturally. We show the power of stable expression, highlighting the usefulness of the cell-based multiplexed approach, which we have adapted to two independent assays previously developed to monitor (a) the activity of the HIV-1-encoded protease or (b) the cleavage of the HIV-1-encoded envelope protein by the host. Multiplexing was achieved by mixing cells each carrying a different assay or, alternatively, by engineering cells expressing two assays. Multiplexing relies on the robustness of the individual assays and their clear discrimination, further enhancing screening capabilities in an attempt to block proteolytic events required for viral infectivity and spread.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S30379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70685024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Arabinosylation Plays a Crucial Role in Extensin Cross-linking In Vitro 阿拉伯糖基化在体外扩展蛋白交联中起关键作用

Biochemistry Insights Pub Date : 2015-01-01 DOI: 10.4137/BCI.S31353

Yuning Chen, W. Dong, Li Tan, Michael A. Held, M. Kieliszewski

{"title":"Arabinosylation Plays a Crucial Role in Extensin Cross-linking In Vitro","authors":"Yuning Chen, W. Dong, Li Tan, Michael A. Held, M. Kieliszewski","doi":"10.4137/BCI.S31353","DOIUrl":"https://doi.org/10.4137/BCI.S31353","url":null,"abstract":"Extensins (EXTs) are hydroxyproline-rich glycoproteins (HRGPs) that are structural components of the plant primary cell wall. They are basic proteins and are highly glycosylated with carbohydrate accounting for >50% of their dry weight. Carbohydrate occurs as monoga-lactosyl serine and arabinosyl hydroxyproline, with arabinosides ranging in size from ~1 to 4 or 5 residues. Proposed functions of EXT arabinosylation include stabilizing the polyproline II helix structure and facilitating EXT cross-linking. Here, the involvement of arabinosylation in EXT cross-linking was investigated by assaying the initial cross-linking rate and degree of cross-linking of partially or fully dearabinosylated EXTs using an in vitro cross-linking assay followed by gel permeation chromatography. Our results indicate that EXT arabinosylation is required for EXT cross-linking in vitro and the fourth arabinosyl residue in the tetraarabinoside chain, which is uniquely α-linked, may determine the initial cross-linking rate. Our results also confirm the conserved structure of the oligoarabinosides across species, indicating an evolutionary significance for EXT arabinosylation.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S31353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70685147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

LIGAND-RECEPTOR INTERACTIONS AND DRUG DESIGN 配体-受体相互作用和药物设计

Biochemistry Insights Pub Date : 2015-01-01 DOI: 10.4137/BCI.S37978

Yanling Zhang, Jianrui Song, Xiaojun Zhang, Yu-Zhi Xiao

引用次数: 4

La3+ Alters the Response Properties of Neurons in the Mouse Primary Somatosensory Cortex to Low-Temperature Noxious Stimulation of the Dental Pulp La3+改变小鼠初级体感皮层神经元对牙髓低温有害刺激的反应特性

Biochemistry Insights Pub Date : 2015-01-01 DOI: 10.4137/BCI.S30752

Yanjiao Jin

{"title":"La3+ Alters the Response Properties of Neurons in the Mouse Primary Somatosensory Cortex to Low-Temperature Noxious Stimulation of the Dental Pulp","authors":"Yanjiao Jin","doi":"10.4137/BCI.S30752","DOIUrl":"https://doi.org/10.4137/BCI.S30752","url":null,"abstract":"Although dental pain is a serious health issue with high incidence among the human population, its cellular and molecular mechanisms are still unclear. Transient receptor potential (TRP) channels are assumed to be involved in the generation of dental pain. However, most of the studies were conducted with molecular biological or histological methods. In vivo functional studies on the role of TRP channels in the mechanisms of dental pain are lacking. This study uses in vivo cellular electrophysiological and neuropharmacological method to directly disclose the effect of LaCl3, a broad spectrum TRP channel blocker, on the response properties of neurons in the mouse primary somatosensory cortex to low-temperature noxious stimulation of the dental pulp. It was found that LaCl3 suppresses the high-firing-rate responses of all nociceptive neurons to noxious low-temperature stimulation and also inhibits the spontaneous activities in some nonnociceptive neurons. The effect of LaCl3 is reversible. Furthermore, this effect is persistent and stable unless LaCl3 is washed out. Washout of LaCl3 quickly revitalized the responsiveness of neurons to low-temperature noxious stimulation. This study adds direct evidence for the hypothesis that TRP channels are involved in the generation of dental pain and sensation. Blockade of TRP channels may provide a novel therapeutic treatment for dental pain.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S30752","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70684840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Withaferin A Inhibits STAT3 and Induces Tumor Cell Death in Neuroblastoma and Multiple Myeloma. Withaferin A抑制STAT3并诱导神经母细胞瘤和多发性骨髓瘤肿瘤细胞死亡。

Biochemistry Insights Pub Date : 2014-11-09 eCollection Date: 2014-01-01 DOI: 10.4137/BCI.S18863

Lisette P Yco, Gabor Mocz, John Opoku-Ansah, André S Bachmann

{"title":"Withaferin A Inhibits STAT3 and Induces Tumor Cell Death in Neuroblastoma and Multiple Myeloma.","authors":"Lisette P Yco, Gabor Mocz, John Opoku-Ansah, André S Bachmann","doi":"10.4137/BCI.S18863","DOIUrl":"https://doi.org/10.4137/BCI.S18863","url":null,"abstract":"Signal transducer and activator of transcription 3 (STAT3) is an oncogenic transcription factor that has been implicated in many human cancers and has emerged as an ideal target for cancer therapy. Withaferin A (WFA) is a natural product with promising antiproliferative properties through its association with a number of molecular targets including STAT3. However, the effect of WFA in pediatric neuroblastoma (NB) and its interaction with STAT3 have not been reported. In this study, we found that WFA effectively induces dose-dependent cell death in high-risk and drug-resistant NB as well as multiple myeloma (MM) tumor cells, prevented interleukin-6 (IL-6)-mediated and persistently activated STAT3 phosphorylation at Y705, and blocked the transcriptional activity of STAT3. We further provide computational models that show that WFA binds STAT3 near the Y705 phospho-tyrosine residue of the STAT3 Src homology 2 (SH2) domain, suggesting that WFA prevents STAT3 dimer formation similar to BP-1-102, a well-established STAT3 inhibitor. Our findings propose that the antitumor activity of WFA is mediated at least in part through inhibition of STAT3 and provide a rationale for further drug development and clinical use in NB and MM. ","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S18863","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32862451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Molecular Differences and Similarities Between Alzheimer's Disease and the 5XFAD Transgenic Mouse Model of Amyloidosis. 阿尔茨海默病与5XFAD转基因小鼠淀粉样变模型的分子异同

Biochemistry Insights Pub Date : 2013-11-21 eCollection Date: 2013-01-01 DOI: 10.4137/BCI.S13025

Chera L Maarouf, Tyler A Kokjohn, Charisse M Whiteside, MiMi P Macias, Walter M Kalback, Marwan N Sabbagh, Thomas G Beach, Robert Vassar, Alex E Roher

{"title":"Molecular Differences and Similarities Between Alzheimer's Disease and the 5XFAD Transgenic Mouse Model of Amyloidosis.","authors":"Chera L Maarouf, Tyler A Kokjohn, Charisse M Whiteside, MiMi P Macias, Walter M Kalback, Marwan N Sabbagh, Thomas G Beach, Robert Vassar, Alex E Roher","doi":"10.4137/BCI.S13025","DOIUrl":"https://doi.org/10.4137/BCI.S13025","url":null,"abstract":"Transgenic (Tg) mouse models of Alzheimer's disease (AD) have been extensively used to study the pathophysiology of this dementia and to test the efficacy of drugs to treat AD. The 5XFAD Tg mouse, which contains two presenilin-1 and three amyloid precursor protein (APP) mutations, was designed to rapidly recapitulate a portion of the pathologic alterations present in human AD. APP and its proteolytic peptides, as well as apolipoprotein E and endogenous mouse tau, were investigated in the 5XFAD mice at 3 months, 6 months, and 9 months. AD and nondemented subjects were used as a frame of reference. APP, amyloid-beta (Aβ) peptides, APP C-terminal fragments (CT99, CT83, AICD), β-site APP-cleaving enzyme, and APLP1 substantially increased with age in the brains of 5XFAD mice. Endogenous mouse tau did not show age-related differences. The rapid synthesis of Aβ and its impact on neuronal loss and neuroinflammation make the 5XFAD mice a desirable paradigm to model AD. ","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S13025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32659268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47