{"title":"姜黄素低聚糖的酶促合成及其抗过敏活性研究","authors":"K. Shimoda, H. Hamada","doi":"10.4137/BCI.S2768","DOIUrl":null,"url":null,"abstract":"Curcumin 4‘-O-glucooligosaccharides were synthesized by a two step-enzymatic method using almond β-glucosidase and cyclodextrin glucanotransferase (CGTase). Curcumin was glucosylated to curcumin 4‘-O-β-D-glucopyranoside by almond β-glucosidase in 19% yield. Curcumin 4‘-O-β-D-glucopyranoside was converted into curcumin 4‘-O-β-glucooligosaccharides, i.e. 4‘-O-β-maltoside (51%) and 4‘-O-β-maltotrioside (25%), by further CGTase-catalyzed glycosylation. Curcumin 4‘-O-β-glycosides showed suppressive action on IgE antibody formation and inhibitory effects on histamine release from rat peritoneal mast cells.","PeriodicalId":8791,"journal":{"name":"Biochemistry Insights","volume":"3 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/BCI.S2768","citationCount":"12","resultStr":"{\"title\":\"Enzymatic Synthesis and Anti-Allergic Activities of Curcumin Oligosaccharides\",\"authors\":\"K. Shimoda, H. Hamada\",\"doi\":\"10.4137/BCI.S2768\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Curcumin 4‘-O-glucooligosaccharides were synthesized by a two step-enzymatic method using almond β-glucosidase and cyclodextrin glucanotransferase (CGTase). Curcumin was glucosylated to curcumin 4‘-O-β-D-glucopyranoside by almond β-glucosidase in 19% yield. Curcumin 4‘-O-β-D-glucopyranoside was converted into curcumin 4‘-O-β-glucooligosaccharides, i.e. 4‘-O-β-maltoside (51%) and 4‘-O-β-maltotrioside (25%), by further CGTase-catalyzed glycosylation. Curcumin 4‘-O-β-glycosides showed suppressive action on IgE antibody formation and inhibitory effects on histamine release from rat peritoneal mast cells.\",\"PeriodicalId\":8791,\"journal\":{\"name\":\"Biochemistry Insights\",\"volume\":\"3 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.4137/BCI.S2768\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry Insights\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4137/BCI.S2768\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4137/BCI.S2768","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
摘要
以杏仁β-葡萄糖苷酶和环糊精葡聚糖转移酶(CGTase)为原料，采用两步法合成姜黄素4′- o -低聚葡萄糖。用杏仁β-葡萄糖苷酶将姜黄素糖化为姜黄素4′-O-β- d -葡萄糖苷，产率19%。姜黄素4′-O-β- d -葡萄糖吡喃苷经cgase进一步糖基化转化为姜黄素4′-O-β-葡萄糖低聚糖，即4′-O-β-麦芽糖苷(51%)和4′-O-β-麦芽糖三苷(25%)。姜黄素4′-O-β-糖苷对大鼠腹膜肥大细胞的IgE抗体形成有抑制作用，对组胺释放有抑制作用。
Enzymatic Synthesis and Anti-Allergic Activities of Curcumin Oligosaccharides
Curcumin 4‘-O-glucooligosaccharides were synthesized by a two step-enzymatic method using almond β-glucosidase and cyclodextrin glucanotransferase (CGTase). Curcumin was glucosylated to curcumin 4‘-O-β-D-glucopyranoside by almond β-glucosidase in 19% yield. Curcumin 4‘-O-β-D-glucopyranoside was converted into curcumin 4‘-O-β-glucooligosaccharides, i.e. 4‘-O-β-maltoside (51%) and 4‘-O-β-maltotrioside (25%), by further CGTase-catalyzed glycosylation. Curcumin 4‘-O-β-glycosides showed suppressive action on IgE antibody formation and inhibitory effects on histamine release from rat peritoneal mast cells.
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