北京大学学报（医学版）最新文献

{"title":"[Perioperative hyperglycemia predicts poorer prognosis of esophageal squamous cell carcinoma patients treated with esophagectomy].","authors":"Bo Peng, Fangfang Liu, Wei Yang, Ruiping Xu, Lei Chen, Baozhong Li, Xinjia Wang, Ji Ke, Wenlei Yang, Yu He, Zhen Liu, Bolin Hou, Liqun Zhang, Miaoping Lin, Lixin Zhang, Fan Zhang, Fen Cai, Huawen Xu, Mengfei Liu, Ying Liu, Yaqi Pan, Zhonghu He, Yang Ke","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To systematically evaluate the association between perioperative hyperglycemia and postoperative prognosis in esophageal squamous cell carcinoma (ESCC) patients using large-scale, multicenter real-world data.</p><p><strong>Methods: </strong>A total of 5 952 patients with ESCC who underwent radical esophagectomy were consecutively included in this retrospective cohort study from the Anyang Cancer Hospital in Anyang, Henan Province (January 2012 to December 2017) and the Cancer Hospital of Shantou University Medical College in Shantou, Guangdong Province (August 2009 to December 2018). Perioperative fasting glucose data were obtained from the hospital information system. The perioperative period was divided into preoperative and postoperative phases: Preoperative hyperglycemia was defined as a mean fasting glucose level ≥7.0 mmol/L from day 14 to day 2 before surgery, and postoperative hyperglycemia was defined as a mean fasting glucose level ≥7.0 mmol/L from day 2 to day 14 after surgery. The primary outcome was overall survival (OS), and secondary outcomes included 30 d/90 d postoperative mortality and in-hospital complications. Multivariable Cox proportional hazards models were used to assess the association between perioperative hyperglycemia and OS, with adjusted hazard ratios (<i>HR</i>) and 95% confidence intervals (<i>CI</i>) calculated.</p><p><strong>Results: </strong>The maximum follow-up period was 12 years. The prevalence of preoperative and postoperative hyperglycemia was 6.7% and 18.3%, respectively. Patients with preoperative hyperglycemia had a lower 5-year OS rate than those without (57.3% <i>vs.</i> 65.0%), with an adjusted <i>HR</i> of 1.41 (95%<i>CI</i>: 1.19-1.68). The patients with postoperative hyperglycemia also had reduced 5-year survival (61.8% <i>vs.</i> 66.4%), with an adjusted <i>HR</i> of 1.39 (95%<i>CI</i>: 1.22-1.58). Joint analysis showed that compared with patients without hyperglycemia, those with hyperglycemia in either the preoperative or postoperative phase alone had an elevated mortality risk (<i>HR</i>=1.24, 95%<i>CI</i>: 1.07-1.43), while the patients with hyperglycemia in both phases had the highest mortality risk (<i>HR</i>=1.86, 95%<i>CI</i>: 1.49-2.32). Stratified analysis revealed that BMI significantly modified the association between hyperglycemia and adverse prognosis (<i>P</i>_interaction=0.010), with the association being particularly pronounced in patients with BMI ≥24.0 kg/m². Additionally, perioperative hyperglycemia was associated with poorer short-term postoperative outcomes.</p><p><strong>Conclusion: </strong>Perioperative hyperglycemia is an independent risk factor for long-term survival in ESCC patients undergoing curative esophagectomy. These findings suggest that enhanced routine glucose monitoring and control during perioperative management of ESCC may help improve long-term patient outcomes.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"567-574"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Price effects of global vaccine pooled procurement].","authors":"Yuxin Huang, Xiaoyi Yu, Ming Xu","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To evaluate the impact of international pooled procurement mechanisms, which are primarily represented by the United Nations Children ' s Fund (UNICEF) and the Pan American Health Organization (PAHO), on the procurement prices of vaccines.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Based on 14 497 vaccine procurement transaction records collected from 188 different countries spanning the period from 2013 to 2024, this research employed a high-dimensional fixed effects model along with an event study metho-dology to accurately identify the price effects and dynamic temporal trends, while simultaneously conducting a multidimensional heterogeneity analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;(1) The baseline model demonstrated that, when compared to the independent self-procurement conducted by individual nations, utilizing pooled procurement through the UNICEF significantly reduced the average vaccine prices by 27.6% (&lt;i&gt;β&lt;/i&gt;=-0.323, &lt;i&gt;P&lt;/i&gt; &lt; 0.01). The PAHO mechanism similarly exhibited an initial price reduction potential of approximately 30.9% (&lt;i&gt;β&lt;/i&gt;=-0.370, &lt;i&gt;P&lt;/i&gt;=0.052). (2) The event study method strictly validated the parallel trend assumption (joint significance test of pre-treatment coefficients: &lt;i&gt;F&lt;/i&gt;=0.27, &lt;i&gt;P&lt;/i&gt;=0.845). Dynamic tracking revealed that a price reduction of approximately 30.0% (&lt;i&gt;β&lt;/i&gt;=-0.356, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) was achieved exactly in the year of transitioning from self-procurement to pooled procurement, and this reduction effect remained persistently stable in subsequent years. (3) Heterogeneity tests revealed a significant \"pro-poor\" effect of pooled procurement: the price reduction margin obtained by small-scale buyers (38.5%, &lt;i&gt;β&lt;/i&gt;=-0.487, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) was significantly higher than that of large-scale buyers (22.5%, &lt;i&gt;β&lt;/i&gt;=-0.255, &lt;i&gt;P&lt;/i&gt; &lt; 0.01). The marginal price reduction coefficient for non-Global Alliance for Vaccines and Immunization (GAVI) eligible countries (&lt;i&gt;β&lt;/i&gt;=-0.418, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) was substantially larger than that for GAVI eligible countries (&lt;i&gt;β&lt;/i&gt;=-0.118, &lt;i&gt;P&lt;/i&gt; &lt; 0.05). The high-income country group experienced the most substantial price drop (&lt;i&gt;β&lt;/i&gt;=-0.475, &lt;i&gt;P&lt;/i&gt; &lt; 0.01). (4) The supply-side moderating effect analysis indicated that UNICEF' s collective bargaining power maintained robustness across diverse market structures, showing no statistically significant attenuation despite increases in market concentration (interaction term &lt;i&gt;β&lt;/i&gt;=0.095, &lt;i&gt;P&lt;/i&gt;&gt;0.10).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Institutionalized pooled procurement mechanisms are capable of significantly reducing vaccine prices. For those countries that are currently in the immunization financing transition period, as well as those facing high self-procurement benchmark prices, participating in an efficient international pooled procurement platform serves as a critical institutional arrangement to replace external financial aid and to effectively maintain the long-term affordab","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"437-445"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cessation intention and behavior of e-cigarettes and associated factors among 18-44 Chinese adults: Based on protection motivation theory].","authors":"Xiaoyue Zhang, Xiaochen Yang, Haoxiang Lin, Ying Ji, Wangnan Cao, Chun Chang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Based on protection motivation theory (PMT), this study aimed to examine the current status of e-cigarette cessation intention and cessation behavior, as well as their associated factors, among adult e-cigarette users in China.</p><p><strong>Methods: </strong>This was a cross-sectional study. The data were derived from a nationwide \"E-cigarette Use Survey\" conducted in 2023, covering 31 provinces (municipality, autonomous region) in China. A total of 550 current e-cigarette users aged 18-44 years were selected as the study population. After adjusting for sociodemographic variables, multiple linear regression models were used to identify factors associated with e-cigarette cessation intention, while binary Logistic regression models were applied to examine factors associated with e-cigarette cessation behavior. In addition, subgroup analyses were conducted according to smoking status to explore potential differences among exclusive e-cigarette users and dual users of e-cigarettes and combustible cigarettes.</p><p><strong>Results: </strong>The mean score of e-cigarette cessation intention was 3.52±0.96, and 31.64% of participants reported having attempted to quit e-cigarettes. After adjustment for covariates, cessation intention was associated with both the threat appraisal and coping appraisal pathways within the PMT framework. Specifically, in the threat appraisal pathway, severity (<i>β</i>=0.14) and vulnerability (<i>β</i>=0.12) were positively associated with cessation intention, whereas perceived extrinsic rewards (<i>β</i>=-0.12) were negatively associated. In the coping appraisal pathway, both response efficacy (<i>β</i>=0.26) and self-efficacy (<i>β</i>=0.28) were positively associated with cessation intention. Regarding cessation behavior, only severity (<i>OR</i>=1.58) in the threat appraisal pathway was positively associated, and cessation intention was also positively associated with cessation behavior. Subgroup analyses further showed that, regardless of smoking status, cessation intention was positively associated with both the threat appraisal pathway (<i>β</i>=0.20, <i>β</i>=0.31) and the coping appraisal pathway (<i>β</i>=0.23, <i>β</i>=0.21).</p><p><strong>Conclusion: </strong>PMT provides a valuable theoretical framework for understanding e-cigarette cessation among adults. Factors related to the threat appraisal pathway are associated not only with cessation intention but also with actual cessation behavior. More-over, the associations of PMT-related factors vary across different smoking status subgroups. These findings suggest that future e-cigarette control policies and interventions could focus on enhancing risk perception and cognitive awareness, and develop more targeted cessation strategies for specific subgroups.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"575-583"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Association between wearable-derived physical activity patterns and gut microbiota in older adults].","authors":"Jiaqi Gao, Wenpeng Li, Xiaoyi Li, Yinxi Tan, Yixin Li, Liping Duan, Tao Wu, Dafang Chen, Yonghua Hu, Mengying Wang","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To identify real-world physical activity patterns in older adults using objective measurements from wearable devices, and to analyze the associations between these patterns and gut microbiota composition.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Based on data collected from a real-world health management project, a total of 743 participants from Eastern, Central, and Northern China were enrolled between January 2018 and June 2025. A 180-day objective physical activity dataset prior to fecal sampling was collected via smart wearable devices to extract features including mean daily steps, coefficient of variation of steps, and the proportion of active days. Fecal samples underwent 16S ribosomal RNA (rRNA) gene (V3-V4 region) amplicon sequencing to obtain genus-level relative abundance matrices. Covariates, including demographics, lifestyle, and chronic disease history, were collected via questionnaires and physical examinations. The discriminative dimensionality reduction via learning a tree (DDRTree) algorithm combined with K-means clustering was applied to identify physical activity phenotypes. Alpha diversity was evaluated using the Shannon index (Kruskal-Wallis test), and beta diversity was assessed using covariate-adjusted permutational multivariate analysis of variance (PERMANOVA) based on Bray-Curtis distance. Multivariable linear regression with false discovery rate (FDR) correction was used to screen differential taxa. A microbial risk score (MRS) was constructed based on taxa with a raw &lt;i&gt;P&lt;/i&gt; &lt; 0.05, defined as the difference between the standardized abundance of beneficial and harmful taxa. Co-occurrence networks were constructed to evaluate micro-ecological topological structures.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The cohort comprised 381 (51.3%) individuals aged 60-74 years and 362 (48. 7%) aged ≥75 years. Compared with the 60-74 group, the ≥75 group had higher prevalences of hypertension (45.9% &lt;i&gt;vs&lt;/i&gt;. 36.7%, &lt;i&gt;P&lt;/i&gt;=0.045) and heart disease (34.0% &lt;i&gt;vs&lt;/i&gt;. 25.2%, &lt;i&gt;P&lt;/i&gt;=0.032), higher systolic blood pressure (median 130 mmHg &lt;i&gt;vs&lt;/i&gt;. 120 mmHg, &lt;i&gt;P&lt;/i&gt; &lt; 0.001), and fewer mean daily steps (median 6 200 steps &lt;i&gt;vs&lt;/i&gt;. 7 000 steps, &lt;i&gt;P&lt;/i&gt; &lt; 0.001). Clustering identified three activity patterns: active group (&lt;i&gt;n&lt;/i&gt;=143, 19.2%; high steps, low variation, high adherence), moderate group (&lt;i&gt;n&lt;/i&gt;=429, 57.7%), and irregular group (&lt;i&gt;n&lt;/i&gt;=171, 23.0%; low steps, high variation, low adherence). The active group exhibited the lowest prevalences of hypertension (35.0%) and heart disease (21.7%), and the lowest systolic blood pressure (mean 124.4 mmHg), whereas the irregular group showed the highest values (51.5%, 40.4%, and 127.6 mmHg, respectively). Alpha diversity showed no significant differences among the groups. After adjusting for covariates, physical activity patterns showed no statistically significant effect on beta diversity (&lt;i&gt;R&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt;=0.003 7, &lt;i&gt;P&lt;/i&gt;=0.115). Compared with the irr","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"551-559"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of LncRNA DANCR on the immune microenvironment of glioma cells by regulating the miR-656/BMPR1A axis].","authors":"Ouyang Wang, Penglei Zhu, Jie Lin, Hao Wu","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the effect of long non-coding RNA (LncRNA) differentiation antagonizing non-protein coding RNA (DANCR) on the immune microenvironment of glioma cells by regulating the miR-656/bone morphogenetic protein receptor type 1A (BMPR1A) axis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The expression levels of DANCR, miR-656 and BMPR1A in glioma cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The U87 cells were transfected or co-transfected to form the following groups: sh-DANCR (transfected with sh-DANCR), overexpression (pcDNA 3.1) DANCR (transfected with pcDNA 3.1 DANCR), NC sh (transfected with negative control sh), pcDNA 3.1 (transfected with pcDNA 3.1 vector), sh-DANCR + miR-656 inhibitor (co-transfected with sh-DANCR and miR-656 inhibitor), sh-DANCR + NC inhibitor (co-transfected with sh-DANCR and NC inhibitor), sh-DANCR + pcDNA 3.1 BMPR1A (co-transfected with sh-DANCR and pcDNA 3.1 BMPR1A), and sh-DANCR + pcDNA 3.1 (co-transfected with sh-DANCR and pcDNA 3.1 BMPR1A). The untreated U87 cells were used as the blank group. The proliferation of U87 cells was detected by CCK-8; invasion and migration were detected by Transwell assay; apoptosis was detected by flow cytometry; the expression of DANCR, miR-656, and BMPR1A mRNA in cells was detected by qRT-PCR; the targeting relationship between DANCR and miR-656 was verified by dual-luciferase; and BMPR1A and immune escape factors [programmed death receptor 1 (PD-1) and programmed death-ligand 1 (PD-L1)] were detected by Western blot.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The mRNA expressions of DANCR and BMPR1A in U87, A172, LN229 and U251 cells were significantly increased, while the expression of miR-656 was significantly decreased compared with those in NHA cells (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). Compared with the blank group and sh-DANCR group, the proliferation rate, invasion, migration number, DANCR, BMPR1A mRNA, BMPR1A, PD-1, PD-L1 expression of U87 cells in the sh-DANCR group were obviously reduced, while the apoptosis rate and miR-656 expression were obviously increased (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). Compared with the pcDNA 3.1 group, the proliferation rate, invasion, migration number, DANCR, BMPR1A mRNA, BMPR1A, PD-1, and PD-L1 expression of U87 cells in the pcDNA 3.1 DANCR group were obviously increased, while the apoptosis rate and miR-656 expression were obviously reduced (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). Compared with the sh-DANCR +NC inhibitor group, the proliferation rate, invasion, migration number, BMPR1A mRNA, BMPR1A, PD-1, and PD-L1 expression of U87 cells in the sh-DANCR+miR-656 inhibitor group were obviously increased, and the apoptosis rate and miR-656 expression were obviously reduced (&lt;i&gt;P&lt;/i&gt; &lt; 0.05), while the expression of DANCR was not obvious (&lt;i&gt;P&lt;/i&gt;&gt;0.05). Compared with the sh-DANCR+pcDNA 3.1 group, the proliferation rate, invasion, migration number, BMPR1A mRNA, BMPR1A, PD-1, and PD-L1 expression of U87 cells in the sh-DANCR+pcDNA 3.1 BMPR1A group o","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"616-623"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular characteristics for poor prognosis related renal cell carcinoma with lymph metastases].","authors":"Fan Shu, Liyuan Ge, Hanzhang Deng, Haoming Yin, Junyong Ou, Shaohui Deng, Yichang Hao, Min Lu, Zhanyi Zhang, Peichen Duan, Shudong Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Next-generation sequencing (NGS) technology was used to analyze the gene mutation profile of lymph node metastases in renal cell carcinoma, and the molecular characteristics associated with poor prognosis were found, providing new ideas for mechanism research and treatment.</p><p><strong>Methods: </strong>Retrospective clinical data collection was conducted on 31 patients with lymphoid metastatic renal cell carcinoma and 21 patients with non-metastatic renal cell carcinoma. A total of 81 formalin-fixed paraffin-embedded tissue samples were retrieved from the Department of Pathology, including primary tumor, lymph node metastasis, and distant metastasis samples. The gene mutation profiles of the patients were examined using next-generation sequencing technology. The patients were followed up to analyze the correlation between lymph node metastasis and patient prognosis.</p><p><strong>Results: </strong>The lymph node metastasis group showed differences in tumor size (<i>P</i>=0.006), World Health Organization (WHO)/International Society of Urological Pathology (ISUP) grade (<i>P</i>=0.002), T stage (<i>P</i>=0.003) and tumor thrombus (<i>P</i>=0.025) compared with non-metastatic renal cell carcinoma. The most commonly mutated genes in our cohort were the tumor suppressor genes <i>VHL</i> (38%), <i>PBRM1</i> (22%), and <i>SETD2</i> (20%). More-over, copy number variations were associated with tumor metastasis, and some mutation features were highly similar to known mutation patterns. There was a difference in mutation frequency between the patients in the metastasis group and samples in the non-metastasis group. The mutation frequency of most genes in the metastasis group was higher, however, Reactome pathway enrichment analysis did not show statistically significant differences in the shared enriched pathways between the two groups. There was a strong degree of concordance between the tumor' s primary and metastatic foci in the same patient, and genomic indicators [such as purity, ploidy, weighted-genomic integrity index (WGII), and intra-tumor heterogeneity (ITH)] as well as clonal and subclonal composition analysis further supported this consistency. The overall survival (OS) was higher in the patients without metastases (<i>P</i>=0.041), and specific gene mutations (such as <i>IGF2R</i>, <i>JUN</i>, <i>EPHA5</i>, and <i>FH</i>) were associated with poorer prognosis. To facilitate distant metastasis, lymph nodes might function as a \"metastatic pool\".</p><p><strong>Conclusion: </strong>The multigene NGS evaluates multiple relevant markers simultaneously, revealing several genetic alterations in the patients with lymphatic metastatic renal cell carcinorma. NGS-based molecular analysis can assist clinicians in assessing a patient' s prognosis and identifying novel, potentially therapeutic mechanisms.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"631-640"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A new method for extracting adult mouse cardiac fibroblasts more efficiently and stably].","authors":"Xiaojuan Ma, Hao Wang, Xueqin Ma, Ying Song, Jiahui Yu, Yan Sun, Yanfang Li, Lixiang Xue, Xianlong Li, Jianling Yang, Yan Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Cardiac fibroblasts (CFs) play a central role in myocardial remodeling and fibrosis. Efficient isolation of CFs is a prerequisite for investigating related mechanisms. However, current methods for isolating primary adult mouse CFs suffer from prolonged processing time, low yield, and poor viability. This study aims to establish a rapid, high-yield, and stable isolation protocol for adult mouse CFs by optimizing the synergistic effect of enzymatic digestion and mechanical dissociation parameters.</p><p><strong>Methods: </strong>Using the gentleMACS^® Octo Dissociator with Heaters, we selected different types and concentrations of collagenase, trypsin, and nuclease as the enzymatic digestion system for CFs extraction. We explored the optimal extraction conditions and compared the results with the commercial Multi Tissue Dissociation Kit 2. The cell yield was quantified using a high-content imaging analysis system by counting the number of adherent cells per field after 72 h of culture. The CFs purity was assessed using immunofluorescence staining for vimentin. The trans-differentiation activity of the CFs was evaluated with transforming growth factor β1 (TGF-β1).</p><p><strong>Results: </strong>Omitting any component of the digestion solution (collagenase Ⅱ/Ⅳ, trypsin or DNaseⅠ), significantly prolonged extraction time and reduced cell yield. In contrast, the optimized protocol outperformed the commercial kit, reducing digestion time by 32.2 min and significantly increasing cell yield, and with comparable obtained CFs purity. After TGF-β1 stimulation, CFs exhibited enhanced proliferative capacity and upregulated expression of α-smooth muscle actin (α-SMA), collagen type Ⅰ (ColⅠ), and fibronectin (FN), confirming the differentiation potential of CFs isolated via the optimized method.</p><p><strong>Conclusion: </strong>This study systematically optimized an enzymatic digestion method combining collagenase, trypsin, and nuclease in conjunction with mechanical dissociation using a tissue dissociator, leading to the efficient and stable isolation of adult mouse CFs. By fine-tuning enzyme concentrations and digestion conditions, we successfully reduced processing time, improved cell yield, and enhanced cell viability compared with conventional isolation methods. These findings validate the physiological relevance of the isolated CFs and demonstrate that the optimized protocol provides a reliable and reproducible method for studying myocardial fibrosis and remodeling. This protocol can serve as a valuable tool for researchers investigating CFs biology and its role in cardiovascular diseases.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"658-665"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Association analysis between genetic nurturing effects of <i>CTNNA</i> gene family and ischemic stroke].","authors":"Jingxian Wu, Liuyan Zheng, Huan Yu, Huairong Wang, Shuting Xie, Yalin Chen, Teng Li, Mengying Wang, Xueying Qin, Tao Wu, Dafang Chen, Yiqun Wu, Yonghua Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the genetic nurture effect of parental genotypes on the risk of ischemic stroke (IS) in offspring and to elucidate the parental origin-specific differences in this effect.</p><p><strong>Methods: </strong>This study utilized data from the \"Family Cohort of Common Chronic Non-communicable Diseases in Rural Areas of Northern China\". A total of 530 core families and sibling pairs were selected, comprising 1 005 offspring. Single nucleotide polymorphisms (SNPs) within the <i>CTNNA</i> gene family (<i>CTNNA1</i>, <i>CTNNA2</i> and <i>CTNNA3</i>) were detected. Using offspring as the unit of analysis, parental non-transmitted alleles were inferred based on Mendelian inheritance principles. Rigorous quality control was implemented for genotype imputation, ensuring high reliability of the inferred data. Linear mixed-effects models were constructed to estimate the genetic nurture effect of non-transmitted alleles on offspring IS. These models compared differences between genetic nurture effects and individual genetic effects, distinguished between paternal and maternal effects, and calculated the statistic <i>η</i> to assess the relative magnitude of parental effects.</p><p><strong>Results: </strong>A total of 1 005 offspring from 530 families were included, comprising 308 IS patients (30.6%) with a mean age of 56.3 years. Sixteen independent SNPs associated with IS genetic nurture effects were identified (9 in <i>CTNNA2</i>, 6 in <i>CTNNA3</i>, and 1 in <i>CTNNA1</i>). The effect sizes ranged from -0.282 to 0.480, with rs117741773 (<i>CTNNA2</i>) showing the strongest effect (0.480, 95%<i>CI</i>: 0.278-0.682). Only four of these SNPs exhibited concurrent individual genetic effects, which acted in the opposite direction to the genetic nurture effects. Parent-of-origin specific analysis revealed that 12 SNPs exhibited genetic nurture effects from a single origin: 4 showed exclusively paternal effects (effect size: -0.298 to 0.945; <i>η</i>: 1.21 to 63.83), and 8 showed exclusively maternal effects (effect size: -0.489 to 0.602; <i>η</i>: 0.03 to 0.44).</p><p><strong>Conclusion: </strong>This study provides evidence that multiple IS susceptibility loci within the <i>CTNNA</i> gene family exhibit significant genetic nurture effects. The findings highlight the complex interplay between inherited genetics and the family environment. The heterogeneity of these effects based on parental origin underscores the significant role of parent-specific genetic nurture in the etiology of IS, offering new insights for understanding the missing heritability in stroke genetics.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"528-535"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Spatial accessibility and optimization of pediatric healthcare resources in Beijing].","authors":"Jiawei Zhang, Zheng Zhu, Chao Gong, Runzhi Han, Li Yang","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To assess the spatial accessibility of pediatric healthcare resources in Beijing and to develop an optimization model for resource allocation under a fixed additional resource constraint, with the aim of exploring optimal allocation strategies for 2025 and 2030.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Using communities as the unit of analysis, this study integrated data on Beijing ' s child population in 2020 and pediatric healthcare resources in 2022. An improved two-step floating catchment area (2SFCA) method was applied to measure spatial accessibility. Based on projected child population data for 2025 and 2030, an optimization model was constructed to minimize regional disparities in accessibility. Under the constraint of a fixed total number of additional resources, optimal spatial allocation schemes were derived and compared with a conventional population-based allocation approach.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In 2022, Beijing had 4 704 pediatric beds and 4 011 pediatric physicians. The mean spatial accessibility for pediatric beds and pediatric physicians was 1.17 and 0.97, respectively, with a standard deviation of 2.78 for bed accessibility, exhibiting a clear spatial pattern of higher accessibility in central districts and lower accessibility in suburban districts. In the same year, the number of pediatric physicians per 1 000 children in Beijing reached 1.52, already exceeding the targets for 2025 and 2030; therefore, no additional increase in total physician numbers was required. Under the 2025 optimization scenario, the mean accessibility of pediatric beds increased to 1.68, with the standard deviation declining to 2.45, indicating a reduction in regional disparities. Under the 2030 scenario, the mean accessibility further increased to 2. 31, with a standard deviation of 2.56, reflecting continued improvement in accessibility. The optimization model identified Daxing District, Tongzhou District, and Mentougou District as priority districts for additional bed allocation, whereas the conventional population-based approach allocated more resources to Daxing District, Haidian District, and Tongzhou District. While the two approaches showed general consistency in overall spatial allocation, the optimization model more effectively addressed inter-district disparities in accessibility.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Significant spatial disparities were identified in the distribution of pediatric healthcare resources in Beijing. The accessibility-oriented optimization approach, under a fixed resource constraint, improved the alignment between supply and demand and reduced regional inequities. It served as a useful complement to conventional population-based allocation methods and provided quantitative evidence to support refined planning and dynamic adjustment of pediatric healthcare resources. Given that the total number of pediatric physicians has already met national targets, leveraging integrated medical con","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"472-478"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical and imaging characteristics and etiology of 544 cases with chronic sialadenitis].","authors":"Jing Yang, Xiaoyun Xu, Danni Zheng, Xiaotong Ling, Liuyang Qu, Denggao Liu","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the etiology of chronic sialadenitis, and to analyze their clinical and imaging characteristics.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This retrospective analysis reviewed the clinical and imaging data of patients with chronic sialadenitis who underwent sialendoscopy at the Peking University Hospital of Stomatology between January 2021 and August 2023.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Inclusion criteria: &lt;/strong&gt;(1) with a history of recurrent swelling of major salivary glands; (2) complete medical records with detailed information about potential causes; (3) sialography images were available; and (4) patients had undergone interventional endoscopy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Exclusion criteria: &lt;/strong&gt;(1) salivary stones; (2) juvenile recurrent parotitis; (3) IgG4-related sialadenitis; (4) Sjögren syndrome; and (5) neoplastic diseases. Based on the latest research results and clinical data, chronic sialadenitis was classified into radioactive iodine-induced sialadenitis (RAIS), allergy-related sialodochitis (ARS), adult chronic recurrent parotitis (ACRP), sialadenosis with sialodochitis, and idiopathic sialadenitis. Idiopathic sialadenitis was defined as a type of chronic sialadenitis with duct stenosis of unknown etiology (allergic causes, autoimmune disorders, radioactive iodine exposure, history of \"parotitis\" in childhood, &lt;i&gt;etc&lt;/i&gt;.). The proportions of five types of sialadenitis were calculated, and their relationships with age, gender, type of affected glands, number of affected glands, duration of symptoms, and imaging characteristics were statistically analyzed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 544 consecutive patients diagnosed with chronic sialadenitis were enrolled in this study. They consisted of 192 males and 352 females, and their ages ranged from 14 to 83 years [mean age: (47.44±13.52) years]. Idiopathic sialadenitis accounted for the largest proportion (71.7%, 390 cases), followed by ARS (12.5%, 68 cases), RAIS (6.4%, 35 cases), ACRP (4.8%, 26 cases), and sialadenosis with sialodochitis (4.6%, 25 cases). Among the 2 176 available glands of the 544 patients, 1 120 (51.5%) glands were affected, including 880 (78.6%) parotid glands and 240 (21.4%) submandibular glands. These five types of sialadenitis exhibited significant differences in gender, age, type and number of affected glands, and duration of symptoms (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). Among them, RAIS patients showed the lowest male to female ratio (male ∶ female=1 ∶ 4.83), ARCP patients were the youngest [(32.50±8.60) years], and RAIS and ARS patients had relatively higher number of affected glands. Sialography showed ductal atresia in 23.2% of affected glands with ARIS, \"snowflake\" pattern in 46.5% of affected glands with ARS, \"punctate and globular\" ectasia of terminal ducts in 80.4% of affected glands with ARCP, and clustered branch ducts in 71.4% of affected glands with sialadenosis with sialodochitis. Moreover, stenosis and/or dilatation of the main and branch","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"58 3","pages":"650-657"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}